Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,500,376	Δ1 bp	5.8%	coding (74/981 nt)	ydcK ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,500,376	0	A	.	5.8%	216.0 / 6.5	69	coding (74/981 nt)	ydcK	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base . (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-02

CTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGC  >  NC_000913/1500076‑1500661                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                              cTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGtaata                                                                                                                                                                                                                                                                                               >  2:387471/1‑301 (MQ=255)          ccGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATCGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTACCCAACCGTTAAAATCCCCAATGCCCATAATCTGCCGTAATAACCCAtttt                                                                                                                                                                                                                                                                                      >  1:322257/1‑301 (MQ=255)                tccgatctgTCATTAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCGCCCCACAGCACGCTGGTGCCGGGGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAAGGGCGATAATCTGCCGTAATAACACATTTTTTTTAg                                                                                                                                                                                                                                                                                <  1:391965/293‑1 (MQ=255)                tcTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTACGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGACGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAg                                                                                                                                                                                                                                                                                <  2:454834/300‑1 (MQ=255)                   aaTGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTACTAACACATTTTTTTTAgaga                                                                                                                                                                                                                                                                             >  1:454270/1‑298 (MQ=255)                       tgTCATGAATTGTGACGCTGTCACTTATATATGCACCCTGGCTTATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATc                                                                                                                                                                                                                                                                         <  2:179853/300‑1 (MQ=255)                        gTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAgagatcgga                                                                                                                                                                                                                                                                        >  2:392479/1‑293 (MQ=255)                             cccgactttcttcctatcTTTTATATGCACCCTGGCTGATTTCCCTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTg                                                                                                                                                                                                                                                                   <  1:338319/284‑1 (MQ=255)                                   ggctgcttttacgtctttatGCTACCTGGTTTCTTGCACTAGTATCGATCTGGAAGTTATCCATTGCGTAAACGCTACCCCCA‑AGCACGCTGGTGTGGGTGATACGCGGATTACCGGAAATCACCGAGCCGCCGAAGGCATTAGCATGCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTGCGCGATCGATCCAGACGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACt                                                                                                                                                                                                                                                             <  1:485349/284‑1 (MQ=255)                                               ggagttcagtcgtgtgctcttccgatcTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTg                                                                                                                                                                                                                                                 <  2:329089/275‑1 (MQ=255)                                                tatataTGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCAACTTCCTGagatcggaagagcacacga                                                                                                                                                                                                                                                >  2:338782/1‑283 (MQ=255)                                                 atatatGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTCATGGCGATAATCTGCCGTAATAAACCATTTTTTTTCGTGCCATCTTCCTGAAAACTAAAGCCCCGCCGtt                                                                                                                                                                                                                                               >  1:245688/1‑301 (MQ=255)                                                       gCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCATAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAACATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTTCCATCTTCCTGATAACTAAAGGCACGCTGttcttccc                                                                                                                                                                                                                                         >  1:422435/1‑299 (MQ=255)                                                                 tGATTTCACTATTATCGATCCAGACGTTATCCGTTGCATAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGata                                                                                                                                                                                                                               <  2:422998/301‑1 (MQ=255)                                                                       cACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTgcg                                                                                                                                                                                                                         >  2:581984/1‑301 (MQ=255)                                                                           attatCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTgagatcggaaaagcgtcgtgtagggaaag                                                                                                                                                                                                                     >  1:328685/1‑273 (MQ=255)                                                                                    ccAGTCTTTATCCTTTGCGTAAAC‑CTCTCCCCACATCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACTCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTcc                                                                                                                                                                                                            <  1:355096/301‑1 (MQ=255)                                                                                    ccAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTACGCATAGCGTTTTcc                                                                                                                                                                                                            >  2:418517/1‑301 (MQ=255)                                                                                          tttATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAgg                                                                                                                                                                                                      <  2:322661/300‑1 (MQ=255)                                                                                            tttCCGTTGCGTAAAC‑CCCTCCCCACCGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTg                                                                                                                                                                                                    <  1:581260/299‑1 (MQ=255)                                                                                             aTCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGa                                                                                                                                                                                                   >  2:625986/1‑301 (MQ=255)                                                                                               ccGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATACTCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACt                                                                                                                                                                                                 >  2:386695/1‑301 (MQ=255)                                                                                                    gCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTNCACTTAGACGATATTTGCGCATANNNNNTNCCAACAGGTGACTTACta                                                                                                                                                                                            >  2:607818/1‑301 (MQ=255)                                                                                                                tcCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTACACGATATTTGCGCATAGCTTTTTCCCACCAGAGACTTACTAAAACCGTAACa                                                                                                                                                                                 >  1:373870/1‑301 (MQ=255)                                                                                                                              gatttccGGTAATACGCGTATCACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGCTATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGAAAATTCTGCGGcact                                                                                                                                                                   >  1:338688/6‑301 (MQ=255)                                                                                                                                  gCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTAGCt                                                                                                                                                               >  2:378670/1‑301 (MQ=255)                                                                                                                                   ccGGTTGTACTCGTATTACCGGAATTCACCGCTTCGGCTAACGCAATAGCATTCTGGTCGTAATTCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCATCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTa                                                                                                                                                              <  1:417954/301‑1 (MQ=255)                                                                                                                                     ggTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCCCTGGCTAtt                                                                                                                                                            >  1:142912/1‑300 (MQ=255)                                                                                                                                                   tACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTACAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGCCTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTcc                                                                                                                                              >  1:481330/1‑301 (MQ=255)                                                                                                                                                    aCCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATACAGCAATTTCCTGCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCa                                                                                                                                             <  1:532314/301‑1 (MQ=255)                                                                                                                                                     ccGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCaa                                                                                                                                            >  2:115851/1‑300 (MQ=255)                                                                                                                                                                 gCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTCCTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCAtttt                                                                                                                                >  1:300989/1‑300 (MQ=255)                                                                                                                                                                     accagAATGCTATTGCGTTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAAAAAATTCCATAAAATGCATTTCAAat                                                                                                                            >  1:243248/5‑301 (MQ=255)                                                                                                                                                                      tccgAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAAT‑ACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCACATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAActat                                                                                                                          >  1:479097/2‑301 (MQ=255)                                                                                                                                                                      gccgAACGCAATAGTATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAAT‑ACACATTTTTTTTAGTGCCATCTTCCTGATAACTAACGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCCCAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAAAAAATTCCAAAAAATGCATTTCAAatat                                                                                                                          >  1:461602/1‑301 (MQ=255)                                                                                                                                                                      gccgAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAata                                                                                                                           <  2:339151/301‑1 (MQ=255)                                                                                                                                                                                   tcATTTTTGTCGTAAATCCAGTATTTTCCTTCTTGTGCTAACACCTTTTCGCGATCGATCCAGCCGCCAGCGTTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACA‑TTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTa                                                                                                             <  2:90426/300‑1 (MQ=255)                                                                                                                                                                                   gCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAAtt                                                                                                              <  2:481931/301‑1 (MQ=255)                                                                                                                                                                                    cATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCTAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTATGCGGCTCTGGCGATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACATAATAAATTa                                                                                                             >  2:368218/1‑301 (MQ=255)                                                                                                                                                                                       ccTGGGCTTAAATCCGGCTATTTCCTTCTTGTGATAACACCTGTTCGCGGTCGATGCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCAGCTGCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAAc                                                                                                          <  1:386181/300‑1 (MQ=255)                                                                                                                                                                                               tAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAg                                                                                                  <  2:186481/301‑1 (MQ=255)                                                                                                                                                                                                 aaTCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAACTTCAATAAAATGCATTTCAAATATACTTTATTAATTAAAAAAAATGATTa                                                                                                >  1:330560/1‑301 (MQ=255)                                                                                                                                                                                                           tttCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCa                                                                                      <  2:330965/301‑1 (MQ=255)                                                                                                                                                                                                            ttCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAACGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAATAAAAAGATGCaa                                                                                     >  1:370048/1‑300 (MQ=255)                                                                                                                                                                                                             tCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAAT‑ACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCagag                                                                                   <  2:462166/301‑1 (MQ=255)                                                                                                                                                                                                             tCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAAT‑ACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCagag                                                                                   <  2:479698/301‑1 (MQ=255)                                                                                                                                                                                                               cttcttGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGc                                                                                  <  2:509218/301‑1 (MQ=255)                                                                                                                                                                                                                      tgCTACCACGGTTTCGCGTGTGATCCAGCCGCCTGCTGTTCCTGCTATCACATCGTTAAATTGGCTAATGGCGTTAATCTGCCGTAATAAGACATTTTTTTTAGTGCCATCGTCCTGATAACTAAAGGCTCGCTGGTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAg                                                                           <  1:386957/301‑1 (MQ=255)                                                                                                                                                                                                                       gCTAATCCCGTTTCGCGATCGTTCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGt                                                                          <  1:115727/301‑1 (MQ=255)                                                                                                                                                                                                                                 tttCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTg                                                                <  2:374366/301‑1 (MQ=255)                                                                                                                                                                                                                                   tggggttcgttcTAGCCGCGAGCGGTTCTTGCTATCACTTCGTTAAAAGCGCTAATGTCGATAATCTTCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGcc                                                              <  1:418111/295‑1 (MQ=255)                                                                                                                                                                                                                                   tCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAATGCTAAATTTGcc                                                              >  2:418674/1‑301 (MQ=255)                                                                                                                                                                                                                                    cgcgATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTACACAATTTGACg                                                             >  1:93058/1‑301 (MQ=255)                                                                                                                                                                                                                                       gatcgatcCAGCCTCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCa                                                          <  2:143037/301‑1 (MQ=255)                                                                                                                                                                                                                                       gatcgatcCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAACATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTCAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGcc                                                          >  1:621814/1‑300 (MQ=255)                                                                                                                                                                                                                                           gatcCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAAGca                                                      >  2:359372/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gccAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAAAGCAATAAAGTGCTCAATTTGCCGGAAGGCAACTTTggat                                             >  1:474080/1‑299 (MQ=255)                                                                                                                                                                                                                                                             tcttGCTTTCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAAGAACAC‑GTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTggg                                   <  1:64096/298‑1 (MQ=255)                                                                                                                                                                                                                                                               ttgttATCTCATCTTTAATATTGCGTATGGCGATTATCTGTCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGTTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTgggg                                  <  1:378174/297‑1 (MQ=255)                                                                                                                                                                                                                                                               cTGCTATCACATCGTTAAAATTGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTgggg                                  <  1:358888/301‑1 (MQ=255)                                                                                                                                                                                                                                                                         aTCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGCGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAggga                        >  2:100848/1‑301 (MQ=255)                                                                                                                                                                                                                                                                          tCGTTTAAATCGCTAATGGCGATAATCGTCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAgggat                       <  1:100724/301‑1 (MQ=255)                                                                                                                                                                                                                                                                           cGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCCTAAACTGCATTTCAAATATCCTTTATAAATTAAACAAAATGAGTCAGAAGATGCAGAACGATAAAGTGCTCAATTTGACGGAAGGCTACTTTGGTATTGGGTTGGGGCCGATAgggagg                      >  1:372911/1‑299 (MQ=255)                                                                                                                                                                                                                                                                              gattaTCGCCATTAGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAgggatggga                   >  2:149711/5‑301 (MQ=255)                                                                                                                                                                                                                                                                               gccggtcttAATGGGGGTAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGAGAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAgggatgggat                  <  1:232504/293‑1 (MQ=255)                                                                                                                                                                                                                                                                                aaaTCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGAACGGGAGGGGAtt                 >  1:27271/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                    tgCTAATGGCGATAATTTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGcc             <  2:474681/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       tAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAgggatgggatagatcgga          >  2:232747/1‑293 (MQ=255)                                                                                                                                                                                                                                                                                             cGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAACTATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGATACTTTGGAATTGTGTTGGGGAAGATAGGGATGGGATTTGCATgactgcat    >  1:627508/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                               ttAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATgc  <  2:248922/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                              CTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAC‑CTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAATAGCATTCTGGTCGTAAATCCAGCAATTTCCTTCTTGTGCTAACACCGTTTCGCGATCGATCCAGCCGCCAGCGGTTCCTGCTATCACATCGTTAAAATCGCTAATGGCGATAATCTGCCGTAATAACACATTTTTTTTAGTGCCATCTTCCTGATAACTAAAGGCTCGCTGTTCTTCACTTAGACGATATTTGCGCATAGCGTTTTCCCACAGGTGACTTACTATAACCGTAGCAAATTCTGCGGCTCTGGCTATGCTCGAGAAAATTCCATAAAATGCATTTCAAATATACTTTATAAATTAAACAAAATGAGTAAGAAGATGCAGAGCGATAAAGTGCTCAATTTGCCGGCAGGCTACTTTGGTATTGTGTTGGGGACGATAGGGATGGGATTTGCCTGGCGCTATGC  >  NC_000913/1500076‑1500661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑