Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,956,978 Δ1 bp 7.1% coding (843/894 nt) ilvY ← transcriptional activator of ilvC; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,956,9780T. 7.1% 166.9 / 7.2 56coding (843/894 nt)ilvYtranscriptional activator of ilvC; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (29/22);  new base . (2/2);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

TCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGACCAGTCCGGCTTCTCTACCG  >  NC_000913/3956682‑3957276
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                          
acAACACGTTGGGTACGTACTGGAACATTTCTTTGTTCCACTAGCGCAGCCATGGCCCCGACGACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGcc                                                                                                                                                                                                                                                                                                        <  1:166384/300‑1 (MQ=255)
        ttGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaattg                                                                                                                                                                                                                                                                                                <  2:121617/300‑6 (MQ=255)
        gctctgccgttcgtgAACATTTTTTTGTGCTACTAACGCAGCAATGGCACCGACGACGGGCGCTGACTGGCGGCGTTCGAGCTTGGCGACCATGAACCGTGTGTCGACACCCGGCGGAATGAGCTGGGCGACGGGTGCCACGACGAAACGAATGACCCGGCGTTCAGGTTCTGTGTGGCGGGTGAGGGAGAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAGCGACATTTGTGGTTCGGCAAAATCGTCCAGAATGCCTCAATGAGCGGCTCATGTAGCCTCTTTTTc                                                                                                                                                                                                                                                                                                <  1:370810/287‑1 (MQ=255)
                     ggcaCATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGAGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGGAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGAAGCCGCTTGTTcagaacggaagaga                                                                                                                                                                                                                                                                                   >  2:371306/4‑288 (MQ=255)
                     ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGCTTGCCACGACGAAACCAATAACCCGGCGTTCACGTTCTTTTTGGCGGGATAGGGAAAAATGCCTGATAGCGCTTCGCTTCTCAGGCCTACCCGCGCGACAACGCCATTTGTGGTTCGGCAAACTCTTCCAGACTGCCTCAATTAGCGACTCAACTAGCCCCTTTTTCTGCCCacacaccc                                                                                                                                                                                                                                                                                   >  1:361887/1‑299 (MQ=255)
                        aCATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTAACCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTACATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCa                                                                                                                                                                                                                                                                                >  2:634452/1‑301 (MQ=255)
                           tttCTTTGTTCCACTGTCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGcc                                                                                                                                                                                                                                                                             >  2:8891/1‑300 (MQ=255)
                              ttttGTTTCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGTCGACCATGAACCGGATTTCGAACCCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaattgaggcattctggaagattttgcc                                                                                                                                                                                                                                                                          <  2:348455/300‑28 (MQ=255)
                                     ccACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGATTCGCTTATCAGGCCTACCCGCGCGCCAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACCAACGCCCAGCTCAAACGGCgc                                                                                                                                                                                                                                                                   >  1:34665/1‑300 (MQ=255)
                                         tATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTtc                                                                                                                                                                                                                                                               <  2:535957/301‑1 (MQ=255)
                                            cGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCa                                                                                                                                                                                                                                                            <  2:604820/301‑1 (MQ=255)
                                                     ggCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTgcgc                                                                                                                                                                                                                                                   >  2:281924/1‑301 (MQ=255)
                                                         ccGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTa                                                                                                                                                                                                                                               <  2:577083/301‑1 (MQ=255)
                                                                  ggggcgTACTGGCGGCGTTGGTACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTGTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAAtcatca                                                                                                                                                                                                                                      <  1:106590/298‑1 (MQ=255)
                                                                        tACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCAGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCATTTTCTCATCGCTGAGCTCTAAAATCATCACGCGGt                                                                                                                                                                                                                                >  2:248546/1‑301 (MQ=255)
                                                                         aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGtt                                                                                                                                                                                                                               <  1:255252/301‑1 (MQ=255)
                                                                         aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGAGGtt                                                                                                                                                                                                                               >  2:408219/1‑301 (MQ=255)
                                                                                     cGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCCCCgaacc                                                                                                                                                                                                                   <  2:34716/301‑5 (MQ=255)
                                                                                       aatttGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTTGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCgg                                                                                                                                                                                                                 <  1:633575/298‑1 (MQ=255)
                                                                                                         cGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGACAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGACTTTTCTGCGCACACACGCCAAGCCCAAACGGCGCTTTCTCATCGCTGCCCACTAAAATCATCACGCGGCTACCCACCAGTCAGGCGCCGTTTTccaccaacc                                                                                                                                                                                               >  1:23360/1‑298 (MQ=255)
                                                                                                                cGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGTCGCTTTTTCTGCGCCCACACCCCCAGCTCAACCGGCGTTTTCTCATACCTGCGCTCTAAAACAACCACGCAGTTACGCCCCGGTTCGGGGCTGTTTTCCAGCACCCCTTCCgg                                                                                                                                                                                        >  1:344880/1‑301 (MQ=255)
                                                                                                                     cccGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGcaaca                                                                                                                                                                                   <  2:345343/301‑1 (MQ=255)
                                                                                                                        ggCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACCACGACATGTGTGCTTCGGCAAAATCTTCCAGACTACATCAATTAGCGGCTCATGTAGCCGCTTTTTATGCGCACACACGCCCAGCTCAAACAGCGATTTCTCATCGCTGCGCTCTAAAACAATCACGCAGAGACGCCCCTGTTCAGGTCTGTTTTACAGcaccacccaccgccaacaac                                                                                                                                                                                >  1:434567/1‑287 (MQ=255)
                                                                                                                                           ctaTTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGc                                                                                                                                                             <  2:169402/299‑1 (MQ=255)
                                                                                                                                                gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTAccacca                                                                                                                                                        >  2:307146/1‑301 (MQ=255)
                                                                                                                                                 ccACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGccaccaa                                                                                                                                                       >  2:52512/1‑300 (MQ=255)
                                                                                                                                                  cACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGACACCCACCATc                                                                                                                                                      >  2:538825/1‑301 (MQ=255)
                                                                                                                                                    cgacgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACCCCACAGCCGACTGCCACCACCgc                                                                                                                                                    >  1:76479/1‑300 (MQ=255)
                                                                                                                                                      acgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATACCACGCCACAGCCGACTGccaccaccacat                                                                                                                                                  >  1:548402/1‑295 (MQ=255)
                                                                                                                                                         aaaCCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCCCCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACACTGCCACGCCACAGCCGAGTaccacccacccccacc                                                                                                                                               >  1:421319/1‑291 (MQ=255)
                                                                                                                                                          aaCCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACACCTTCCGGCAACAAAGCCACGCCACAGCCGAGTGCCACCACCGAAACCac                                                                                                                                              >  1:269780/1‑300 (MQ=255)
                                                                                                                                                                     gggggtgaggtttgttgtgggCGGGTTAGGAAAAAAGTCGTGGTTGCGGTTCGTTTATGTGTCTTACCCGCGCGACAACGTCATTGGTGGTTCGGCAAAATCGTCCCGAATGCCGCAGTTAGCGGCTCATGTGGCCGCGTTTGCTGCGCACACACGCCCAGCTCAAACGGCGTTTGCGCATCGCTGCGCTCTAAGATCAGCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACATTGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGcc                                                                                                                                   <  1:334631/282‑1 (MQ=255)
                                                                                                                                                                              ggTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCAACAGCCGAGTGCCACCATCGAAACCATCGCTTAATGCCCACCAAccc                                                                                                                          >  1:203694/1‑300 (MQ=255)
                                                                                                                                                                                      ttGGGGGGTTAGGGAAAAATGCCTGATAGCGCTGCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTaa                                                                                                                  <  1:281621/301‑1 (MQ=255)
                                                                                                                                                                                        ggCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTaaaa                                                                                                                >  2:463161/1‑300 (MQ=255)
                                                                                                                                                                                               tAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTACTGCCCGCCAAACGAGGCGTAAATCAACggg                                                                                                         >  1:274582/1‑301 (MQ=255)
                                                                                                                                                                                                 gggAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGAAACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATACACGGGtt                                                                                                       >  2:304601/1‑301 (MQ=255)
                                                                                                                                                                                                   gAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTAc                                                                                                     >  2:495479/1‑301 (MQ=255)
                                                                                                                                                                                                              taTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTg                                                                                          <  2:203937/300‑1 (MQ=255)
                                                                                                                                                                                                                          tttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACcac                                                                              <  2:549075/300‑1 (MQ=255)
                                                                                                                                                                                                                                 ggCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCACACGGCGTTTTCTCATCGCTGCGCTCTAAACTCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACCGCCGAGTGCCACCATCGCAACCCTCGCCTCATGCCCGCCAAACGCGGCGTACAACATCCGGTTAACTAATTTACTCCCTCAACACCACAGATCaa                                                                       >  1:560265/1‑301 (MQ=255)
                                                                                                                                                                                                                                   tctctctgcgcgACATCGTCTTTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATg                                                                     <  2:560979/294‑1 (MQ=255)
                                                                                                                                                                                                                                       cccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCCCCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAAACATCGGGTTACTGATTTTATTACGTCGAACACACAGTTCAATgcggg                                                                 >  1:453461/1‑300 (MQ=255)
                                                                                                                                                                                                                                          gcgtgACAACGTCATTTGTGTTTCGTCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATgcggcggc                                                              <  2:362371/301‑1 (MQ=255)
                                                                                                                                                                                                                                             ctaCAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTa                                                           <  2:454025/299‑1 (MQ=255)
                                                                                                                                                                                                                                                       aTTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTTCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTg                                                 >  2:406162/1‑301 (MQ=255)
                                                                                                                                                                                                                                                             ggTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAACTCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCCCTTCCGGCACCAATGCAACGCCACAGCCGAGTGCCCACATCGATACCATCCATTCATGCCCCCCAACCGTAGCGTAAATCACCGAGTTACTGACTTTACTGCATCGAAAACACACTACAAAGAGGCTGCCTAACGGccacggctcgcc                                           >  1:102860/1‑291 (MQ=255)
                                                                                                                                                                                                                                                                tCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCCCCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCAAAACCACAGTTCAATGCGGCGGCTGACCGGCCCCttaaccgcccc                                        >  1:335014/1‑291 (MQ=255)
                                                                                                                                                                                                                                                                 cGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCCCCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCCTAAATCAACGGGTTACTGATTTTATTGCGTCGAAACCACCGTTCAAATGGGCGGCGTCCCGGccccccaaccgcccca                                       >  1:334705/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 cGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCata                                       <  2:274885/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                        ttCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACg                                <  2:270078/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAAAAAACGGc                              >  2:376124/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                   ggCCTCAATGATCGGGTCTGGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGAc                     <  1:306742/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      cagaaaaaGCGGCTACATGAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCATTGACCaa                  >  2:580845/8‑300 (MQ=255)
                                                                                                                                                                                                                                                                                           aaaGCGGCTACATGAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGTGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCAAGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAAGGCGGAGGCGTACCGGCCCCTGATCGGCCATAAAAACCGGCACCCGTAACCAGccccg             >  1:266363/3‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                       ggTAGCCGTTTTTTGTGTTCACGCACGTGCAGTTCTGACGGCGTTTTGTCGTGTCTGCGATGTAGAAGCAGCAGGCGGTTAGGGACGGGTTCGGGGCTGTTTTCCAGAACAACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGACCAGTCCGGCTTCTCTACCg  <  1:462597/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                          
TCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGACCAGTCCGGCTTCTCTACCG  >  NC_000913/3956682‑3957276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: