| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 465,185 | Δ1 bp | 6.2% | coding (363/696 nt) | queC ← | 7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 465,185 | 0 | A | . | 6.2% | 206.4 / 6.4 | 65 | coding (363/696 nt) | queC | 7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis |
| Reads supporting (aligned to +/- strand): ref base A (28/33); new base . (2/2); total (30/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
GTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTG > NC_000913/464892‑465481 | ttCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTa < 2:510899/300‑1 (MQ=255) ttATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTAGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCAGTAATAATATGCCAGCGCCCCGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGACTATCTTTCGCCACTCCCAAACTGACGGCATGGTTTAGTGCTTTAACAAACTCATCGCGGCAATACAGGTAGACGGAAAAAACCGTTTAGCAGACGACAATAATTAcaacta > 2:44736/1‑296 (MQ=255) tATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGCCTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGACTATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACACACCCATCGCGGCAATCCGGGTAGCCGGAGAAAACCGTTTCGCAGACGCCAGTAATTAC‑GGCTTc > 1:332006/1‑301 (MQ=255) tCGTCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGGTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTg < 1:597222/301‑1 (MQ=255) cGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTGCATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGc > 2:474538/1‑301 (MQ=255) cGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGAACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAAGCC‑GTAATTACTGGCGTCTGc < 2:618505/301‑1 (MQ=255) ttttcgatcttGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAAGCC‑GTAATTACTGGCGTCTGcg < 2:11552/291‑2 (MQ=255) ggCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAgatcggaaaagaacacgc > 2:636237/1‑284 (MQ=255) aTAATGATTCATACCTTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTAcc < 1:109512/301‑1 (MQ=255) aaaTGTTTCTAACCGTGTGGTCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTGATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCt < 1:404934/300‑1 (MQ=255) tGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCCTGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGCAGCC‑GTAATTACTGGCGTCTGcgaggtcggaag > 1:11552/1‑290 (MQ=255) gATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATa > 2:352838/1‑301 (MQ=255) cAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCa > 2:147230/1‑301 (MQ=255) aaCCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTGATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCata < 2:334713/301‑1 (MQ=255) ggCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTgcc > 2:210342/1‑301 (MQ=255) aCATGCCGAACAATGACCGGAACCGTCGCCTTTAAGGCCGTTTTAGACGGTCAACGTTTCGTTACGGACTAAATCCATTTTGCCTTAATAATCTGCCAGCGCCCAGG‑TGTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTGTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAgg < 1:352375/301‑1 (MQ=255) cGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGccc > 2:38481/1‑301 (MQ=255) aaaCCGTCGCCTTTAAAGTCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTg < 2:233128/300‑1 (MQ=255) aaCCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTgg < 2:491930/301‑1 (MQ=255) aCCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTggg < 2:540645/301‑1 (MQ=255) gatctgCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGa < 2:302643/296‑1 (MQ=255) gCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGaagacc > 1:302239/1‑296 (MQ=255) ggACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGAAACCATCGGCTTCAGGTTCATAACCAgg > 1:125109/1‑301 (MQ=255) aTCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACAGGa > 2:531769/1‑301 (MQ=255) gCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCa > 2:302442/1‑301 (MQ=255) cggtagtatgtggcgagggccAGGTTTTCGGCTTTATCAATCCACATTAGGTGCGTTGCAAAACGAAGTTTTTGTGCCTTGCCCAAACTGACGGCATGGTGTAGTGCTTTCACAAACTCAGCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCAc < 1:433856/282‑1 (MQ=255) taataaTCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCAcgcg < 2:125233/301‑1 (MQ=255) cccAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAgtagcctgacgcg < 2:456586/301‑13 (MQ=255) cccgg‑TTTCCTCTTTATCTATCCACAGCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGAATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGcc < 1:531096/298‑1 (MQ=255) ccAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGcc < 1:38429/301‑1 (MQ=255) ggtgtcggcttgtggatgccACTTCAGTGGCGTTTCAAATCGAATCTCTGTCGCCATGCCCAAACGGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGc < 1:215713/283‑1 (MQ=255) cGCTTTATCAATTCACATCATCTGCGTTGCAAAACGAATATCTTTCGCCATGCCCAAACTGTCGGCATGGTTTAGTGCTTTCACAAACGCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAAGTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATAGTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTg < 1:504206/301‑1 (MQ=255) cGCTTTATCAATCCACTTCGGCTGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTg < 1:224510/301‑1 (MQ=255) cGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAAGCACCGGAATGCTGTCCAGCGTCAGGCCAATGACCGCCAGCTCCttt > 1:294870/1‑300 (MQ=255) gCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGa < 1:210099/301‑1 (MQ=255) aaTTCACAGCAGCGTCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTg < 1:302038/301‑1 (MQ=255) cacaTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACaa > 2:233959/1‑300 (MQ=255) caTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATcc < 1:614616/301‑1 (MQ=255) caTCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACAAcc > 2:615474/1‑301 (MQ=255) gggcggggTTTTAAAGCGAATATCTTTCGCCATGCCCAAAGTTCCGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGGTGCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGc < 1:583552/299‑1 (MQ=255) tttCAAAACGAATAGCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGGGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAAGCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATg < 1:300893/301‑1 (MQ=255) ttCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATgc > 2:451473/1‑301 (MQ=255) tCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCtaaagcc > 1:211495/1‑295 (MQ=255) cAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATgcgc > 2:9002/1‑301 (MQ=255) cAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTATCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCAACTTATgcgc > 2:636826/1‑301 (MQ=255) aaaaCGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCCCCGGAATGCTGTCCCCCGTCAGGCTACTTACCCCCAGCTCGTTGACCCGGGTCACATCCAACACCttcaacacc > 1:67704/1‑294 (MQ=255) aaCGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGACTACTTACCGCCAGCCCGTTGCCCAAGGTGACATCCAGCCCCTTAAgagcgcc > 1:280150/1‑300 (MQ=255) ctaaaccatgccgtcagtttgggCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGCTCATAATCAGGCACCGGAATGCTGTAACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCACCCGTCCCCCCAGt > 1:373614/22‑301 (MQ=255) aggCCCAAATGGACGGGATGGTTTTGTGCTTTCACAAACTCATCGAGGCAATCCGGGTAGCCGGAGAAATCCGTTGCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCGGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAgcg < 1:9002/299‑1 (MQ=255) aTGCCCAAATTGTCGGCTTGGTTTAGTGCTTTCACAAACTCATCGTGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTATCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAgcg < 1:635949/301‑1 (MQ=255) gCCTAAACTGACGGCATTGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGcc < 2:332411/301‑1 (MQ=255) acatgacgcgcttccgattgcgcgGCAATCCGGGTAGCCGGTGAAATCCGTTTCGCAGACGCCAGTAATTAC‑TGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGAtt < 1:576123/281‑1 (MQ=255) gCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTg > 2:371439/1‑301 (MQ=255) cTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTCTGCGCGCGTGCCCCCAGTTTCCGCGCCAGTTCGCGTTCCACGTCGAATTCTgc > 1:198545/1‑301 (MQ=255) cgcgGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGAttagatcggaagagcacacgtc > 2:576838/1‑281 (MQ=255) gcgGCAAGCCGGGTAGCCGGTGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACTCATAGATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCg < 1:370943/301‑1 (MQ=255) ttttgctcttccgatctCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAAc < 2:233194/285‑1 (MQ=255) gtggaGCCGGAGAATTCCGTTTCGAAGACGCCAGTAATTTC‑GTCTTCTGCTTGTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATAGTACGCCCTGGGACAAACGTATTCGGGAGACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCGACGGGCCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGGAATCGAACg < 1:497775/297‑1 (MQ=255) tAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCa < 2:374110/301‑1 (MQ=255) ctgCCGGAGATATCCGGTTTGCTGTCTCCAGTAATTAC‑GGTGTCTTCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCa < 1:48095/299‑1 (MQ=255) gCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAAAGTCaaa > 2:48148/1‑299 (MQ=255) ccGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCCGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAAcagaacggaaacgcggcg > 1:232951/1‑284 (MQ=255) aaaagCAGAAGCC‑GTAATTACTGGCGTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCAGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGACGCTGACCGTAATCGAACGTCACGCAATGGAATtcctc > 1:110750/5‑301 (MQ=255) gACGCCAGTAATTTC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATAttgt < 2:211738/301‑1 (MQ=255) cGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCCCGTCCATTTCCGAGCGATACCCCCGCCCGTAACCGAACGCCACCCAATGGAccctccccttttgttt > 1:7268/1‑286 (MQ=255) | GTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGG‑TTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTAC‑GGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTTCATAATCAGGCACCGGAATGCTGTCACGCGTCAGGCTACTGACCGCCAGCTCGTTGAGCAGGGTGACATCCAGCACCTTATGCGCGCGTGCCCCCAGTTTCAGCGCCAGTTCGCGTGCCACGTCGATTTCTGCGCGATGCCGCTGACCGTAATCGAACGTCACGCAATGGACTTCATCATATTGTTG > NC_000913/464892‑465481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |