Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,679,434 +C 8.8% intergenic (‑67/+30) yphF ← / ← yphG putative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,679,4341.C 8.8% 211.3 / 17.1 57intergenic (‑67/+30)yphF/yphGputative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base . (25/27);  new base C (2/3);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

GGTACTTTCTTTCGAAATATCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAGAAATCCGCTTCGATACTGGTTTTCGCCATCTCTTGCGC  >  NC_000913/2679134‑2679721
                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                               
ggTACTTTCTTTCGAAATATCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATCAAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTACTTATTTTGTGATGAGCGGCcgcgcg                                                                                                                                                                                                                                                                                                  >  1:263715/1‑301 (MQ=255)
    ctttctttCGAAATATCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGcc                                                                                                                                                                                                                                                                                             >  1:570485/1‑301 (MQ=255)
         tttcGAAATATCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGCCAAATAGTGCGCTACCTAATAGCGTAGCATTTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTCGGGTTTTTATTAACACCGGCTTATTCTAATTATTTTTTGATGAGCGGCAGCGCGTGCCGccaa                                                                                                                                                                                                                                                                                        >  1:436253/1‑298 (MQ=255)
          ttcGAAATATCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCat                                                                                                                                                                                                                                                                                       <  2:157096/301‑2 (MQ=255)
             gAAATATCACCCTGGGCGTTTGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAata                                                                                                                                                                                                                                                                                    <  2:431203/301‑1 (MQ=255)
                 tatCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTATAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTAc                                                                                                                                                                                                                                                                                >  1:538231/1‑301 (MQ=255)
                               ttAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGttt                                                                                                                                                                                                                                                                  <  2:571199/301‑1 (MQ=255)
                                 aGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAg                                                                                                                                                                                                                                                                <  2:263983/301‑1 (MQ=255)
                                            aCTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGtt                                                                                                                                                                                                                                                     <  2:538904/301‑1 (MQ=255)
                                                         ggAGGAATCTTTTGCCGCATCCTGACCGTCCTGACGCACGCCTGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCGAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGc                                                                                                                                                                                                                                        <  1:614837/300‑1 (MQ=255)
                                                          gAGGATTCTTTTTCCGCATCCTGAACGCTCTGACGCATTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTGCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCa                                                                                                                                                                                                                                       <  1:421511/301‑1 (MQ=255)
                                                          gAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAACTATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCa                                                                                                                                                                                                                                       >  1:148044/1‑301 (MQ=255)
                                                                 cTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAAGCATCACggt                                                                                                                                                                                                                                >  2:45650/1‑301 (MQ=255)
                                                                  ttttGCCGCATCCTGAACGCCCTTACGCACTCCGGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                                                                                                                                                                                                                               <  1:203686/301‑1 (MQ=255)
                                                                      gCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATAACGGTGGTGa                                                                                                                                                                                                                           >  2:131400/1‑301 (MQ=255)
                                                                          cATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGAATCACGGTGGTaaaaaa                                                                                                                                                                                                                       >  1:103800/1‑301 (MQ=255)
                                                                               tGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCg                                                                                                                                                                                                                  <  2:118115/301‑1 (MQ=255)
                                                                                    ttcagacgtgtgctcttccgatcttCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTc                                                                                                                                                                                                             <  2:541468/277‑1 (MQ=255)
                                                                                      ccTTACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg                                                                                                                                                                                                           <  2:436817/301‑1 (MQ=255)
                                                                                           cGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGATTTCGGCCaaa                                                                                                                                                                                                      >  2:349042/1‑300 (MQ=255)
                                                                                                tCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGa                                                                                                                                                                                                 <  2:112670/301‑1 (MQ=255)
                                                                                                 ccAGCGTAGTATCCCTGAGTATCAAAGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGAt                                                                                                                                                                                                <  2:264813/301‑1 (MQ=255)
                                                                                                            tCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTcagatcggaagagcgtcgggtaggg                                                                                                                                                                                     >  1:540795/1‑277 (MQ=255)
                                                                                                                tcttccgatctgTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAAtt                                                                                                                                                                                 <  2:569639/290‑1 (MQ=255)
                                                                                                                           gTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATCGGGTTTAGTGAACTCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAAttagatcggaaga                                                                                                                                                                      >  1:568925/1‑290 (MQ=255)
                                                                                                                                 tGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCa                                                                                                                                                                >  2:129527/1‑301 (MQ=255)
                                                                                                                                        aTGTTCTTTTCTTTTTCCTAAGCCCTTGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTATGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcg                                                                                                                                                         <  1:131275/301‑1 (MQ=255)
                                                                                                                                         ttgTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcgc                                                                                                                                                        <  2:578068/299‑1 (MQ=255)
                                                                                                                                                  cTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATAGTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGAATTAATCGCCGTCAATTCAGCGCGAGCAGACACATATTGCACAACcccccc                                                                                                                                               >  2:359907/1‑301 (MQ=255)
                                                                                                                                                          gCAGCCCTGTCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcg                                                                                                                                       <  2:148170/301‑1 (MQ=255)
                                                                                                                                                                          gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTAATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGc                                                                                                                       >  2:551868/1‑301 (MQ=255)
                                                                                                                                                                                        ggCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCTTCCTGTAGGGTTTTTATTACCAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAtt                                                                                                         <  1:129403/300‑1 (MQ=255)
                                                                                                                                                                                          tgTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAtttt                                                                                                       <  2:499511/300‑1 (MQ=255)
                                                                                                                                                                                             tgctattattattAGTTTACGTGTTTTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCt                                                                                                    <  2:396585/297‑1 (MQ=255)
                                                                                                                                                                                             ctggagttcagacgtgtgctcttccgatctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCt                                                                                                    <  2:466410/274‑1 (MQ=255)
                                                                                                                                                                                              ggttctaattaTTGTTTTCGGGTTGTTTTTATTTTTGTTGGCATAGTGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTTATTATTTTGGGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCtt                                                                                                   <  1:391831/296‑1 (MQ=255)
                                                                                                                                                                                               ccATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGAGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCCCCACCACCAGGCATTTTTCttt                                                                                                  >  1:393444/1‑301 (MQ=255)
                                                                                                                                                                                                tgtgcttttccgatctcGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTa                                                                                                 <  2:46727/285‑1 (MQ=255)
                                                                                                                                                                                                    aataatAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCAAACATCAGGCATTTTTCttagatcg                                                                                             >  2:392345/1‑295 (MQ=255)
                                                                                                                                                                                                            tttACGGTTTTGTCTCATTTTTGTTGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCtgttgt                                                                                     <  1:551195/300‑1 (MQ=255)
                                                                                                                                                                                                               aCGGGTTGTTCTGATTTTTTTAGGCATTGAGCCTCCTGTAGGGTTTTTATTAAAAACGGCGTATTCTAAGTATTTTTGTAGGGGCGGCAGCGCGTGCCGCTCAAATATTACAGGTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTaaa                                                                                  <  1:348579/301‑1 (MQ=255)
                                                                                                                                                                                                                cGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGCCTCATATTGCGCAACCTCCCCGAGTCCCGCGGACCCCAGCCTCGCCACCAACAGGCATTTTTCTTTaagatcggaacaccgtc                                                                                 >  1:46674/1‑285 (MQ=255)
                                                                                                                                                                                                                           cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCCTTTTTCtcgaacggaagagcgtcgtgtagggaaagcg                                                                      >  1:465846/1‑271 (MQ=255)
                                                                                                                                                                                                                                 tGTAGGCTTTGTGCTTACTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAAGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGacaac                                                                <  1:45597/301‑1 (MQ=255)
                                                                                                                                                                                                                                     ggCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCCATTCAGCGCGAGCAGACTCATATTGAGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCAACAATCAGGCACATTAATTTAAGCTCCTGTTATAACACACCATACCGCCACAACAAgc                                                            >  1:606670/1‑300 (MQ=255)
                                                                                                                                                                                                                                        aTAGAGTCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGACTAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCCAGTCCCGCCGCCGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCAACCAACTACAACAAGTCAg                                                         >  1:107610/1‑301 (MQ=255)
                                                                                                                                                                                                                                        aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAAAAAGTCaa                                                         >  2:190976/1‑300 (MQ=255)
                                                                                                                                                                                                                                        aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCAATACAGCGAcaaaaaataaa                                                         >  2:427485/1‑296 (MQ=255)
                                                                                                                                                                                                                                         tAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAgg                                                        >  1:252590/1‑301 (MQ=255)
                                                                                                                                                                                                                                          agagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGt                                                       <  2:103924/301‑1 (MQ=255)
                                                                                                                                                                                                                                             gCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATCCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTCCTTACTGCTGCTGCTGTAAATCGCCATACAGCAacacaaaacaaggttgc                                                    >  1:291698/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                  tggattttagacgtgttctcttccgatcttgaGCGGCACGCGCTGCCGCTTAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACGCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAGGAATCCGCt                               <  2:373838/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ccgatttaccctattaactttaatatttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAGAAATCCGCTTc                             <  2:485741/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aTTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAGAAATCCGCTTCGATAATGGt                    >  2:304776/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                            atttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAGAAATCCGCTTCGATACTGGTTTTCGCCATCTCttt     >  2:256956/4‑300 (MQ=255)
                                                                                                                                                                                                                                                                                             gatgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAGAAATCCGCTTCGATACTGGTTTTCGCAATCTCTTgc    >  2:401502/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                              ttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCAGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGACACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATTGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAGAAATCAGCTTCGATACTGATTTTCGCCATCTATTgcg   >  2:305690/2‑301 (MQ=255)
                                                                                                                                                                                                                                                                                               ggaGCGGCAGCGCGTGCCGATTAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAGAAATCCGCTTCGATACTGGTTTTCGCCATCTCTTgcgc  <  1:304372/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                               
GGTACTTTCTTTCGAAATATCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAGAAATCCGCTTCGATACTGGTTTTCGCCATCTCTTGCGC  >  NC_000913/2679134‑2679721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: