Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,169,265 +T 8.5% intergenic (‑34/+19) ygiV ← / ← ygiW transcriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,169,2651.T 8.5% 167.4 / 12.1 47intergenic (‑34/+19)ygiV/ygiWtranscriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein
Reads supporting (aligned to +/- strand):  ref base . (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGC  >  NC_000913/3168965‑3169554
                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                 
cacaCCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAAcgcg                                                                                                                                                                                                                                                                                                    >  2:377892/1‑301 (MQ=255)
            aTTTTCGTGGATCGGTTCTCTAACGCTGCCGCAGATATGAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGGCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGACGCGTTCCCGGGaataa                                                                                                                                                                                                                                                                                       <  1:26727/301‑5 (MQ=255)
                          tttCGCTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGAt                                                                                                                                                                                                                                                                         <  2:210689/300‑1 (MQ=255)
                               cTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACCGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCCCAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGAAGCGGTCACAATTACGGATTTACt                                                                                                                                                                                                                                                                    >  2:459859/1‑301 (MQ=255)
                                   cGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCCACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCATGCGAAATTATTCCCGGAAACGCGTCCCGGGAGCGGTAACAATTAAGAATTTAcgtaag                                                                                                                                                                                                                                                                >  1:338606/1‑296 (MQ=255)
                                          gccgATATCAAAGTGAAACGCTTCCGGTGCGGTGGGGTCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTGGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGGGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTg                                                                                                                                                                                                                                                         <  1:339361/298‑1 (MQ=255)
                                            aGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATCAATTTCGCCACGCTGTAGTTGAGCAATTCACGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCCTTTTGCCTCCCTGCGAACTCATTCCCAGGAACGCGTCACGGGAGCGGTAACAATACCGGACATACTTAGCGGacacact                                                                                                                                                                                                                                                       >  1:129863/1‑295 (MQ=255)
                                             gttatCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGttt                                                                                                                                                                                                                                                      <  2:242278/299‑1 (MQ=255)
                                                atCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGaa                                                                                                                                                                                                                                                   >  2:111376/1‑300 (MQ=255)
                                                  aaaaGTGAATCGCTTCCGGTGGGGTGGTGGCAGGGTCGTCCCAGGCGACTCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGt                                                                                                                                                                                                                                                 <  1:377396/300‑1 (MQ=255)
                                                        gAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGCTCTGTTTGACGTCAAttt                                                                                                                                                                                                                                           >  1:616432/1‑301 (MQ=255)
                                                           aCGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCaa                                                                                                                                                                                                                                        <  2:347770/301‑1 (MQ=255)
                                                                    gttgggtggtGGGAGGGTCGTCCCAGGCGGCGCCAAAAGTTTGGCTTTGGTTAACAGGAGAATGCCCCGTCGCTGTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCGACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTGTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTcc                                                                                                                                                                                                                               <  1:459295/297‑1 (MQ=255)
                                                                                cAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCTCAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGa                                                                                                                                                                                                                   >  2:492130/1‑301 (MQ=255)
                                                                                         cccAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCTCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGCTCAGCAATTCAGGCCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTATTTATCGACTTCACCCt                                                                                                                                                                                                          >  2:408280/1‑301 (MQ=255)
                                                                                               tgACGCTAAAAGTTTGGCTTTGGGTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGTTAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACATGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACCTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCAGGAtcg                                                                                                                                                                                                    <  1:407717/300‑2 (MQ=255)
                                                                                               gggagcgaaaagttgggtgtgggTTTACATGAGAAATCCCCGGTTGTTTTCGCCACGTGTTAAATTTCGCCACGCTGTAGTTGAGCAATTCTGGGCTACAGCGGTTCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACTATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAAtct                                                                                                                                                                                                    <  1:335433/280‑1 (MQ=255)
                                                                                                 aCGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCa                                                                                                                                                                                                  <  2:360963/301‑1 (MQ=255)
                                                                                                    ccAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAAAAATTACGGATTTACTTTGCGGATCTTTTTGACGTCCATTTCAACAGAATTCCAGTCTTTCACACCTTCACCCTGAATCCCAAcc                                                                                                                                                                                               >  1:51896/1‑301 (MQ=255)
                                                                                                               gCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCgg                                                                                                                                                                                    <  2:107741/301‑1 (MQ=255)
                                                                                                                                                        tGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCaa                                                                                                                                           >  2:143419/1‑301 (MQ=255)
                                                                                                                                                         gATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGACATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGTACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGAGGATCTGTTTGACGTCAATTTCACCAGAATTCCAGTCTTTACCGACTTCACCCTGAATCGCAACCGTAACTTTCGGCTTCACCGTCACGCCGTTcaacccatcgtgcacgatctcaac                                                                                                                                          >  1:68829/1‑278 (MQ=255)
                                                                                                                                                               tttCGCCACGTTGTAGTTGGGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGAGGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACGTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGAt                                                                                                                                    <  1:530087/301‑1 (MQ=255)
                                                                                                                                                                ttgtccACGGTGTAGTTGTGCAATTCAGGGCTACTGCGGTGCGGCAACAGCGCCTCAGGGATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATa                                                                                                                                   <  1:491484/297‑1 (MQ=255)
                                                                                                                                                                    caccgctgtagccctgaattgctcaacTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGACCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGGGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTAc                                                                                                                               >  2:448581/27‑301 (MQ=255)
                                                                                                                                                                     caggttGTAGTTGGGCAATTCAGGGCTAGAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTAcc                                                                                                                              <  1:111252/296‑1 (MQ=255)
                                                                                                                                                                           gTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCTTCACCCCCTTCCAACCTTTGTGGTCCATAACAACATTTATTGTTCCGCTTGc                                                                                                                        >  1:11683/1‑301 (MQ=255)
                                                                                                                                                                                tGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCtt                                                                                                                   <  2:11683/301‑1 (MQ=255)
                                                                                                                                                                                 gggCAATTTAGGGCTTCAGGGGTGCGGCAACATCGCCACAGGTAGTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCttt                                                                                                                  <  1:143293/299‑1 (MQ=255)
                                                                                                                                                                                            ggCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACATCAATTTCAACAGCATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTAACGCTGGCATCTTTGAACACGTaga                                                                                                       >  1:212678/1‑301 (MQ=255)
                                                                                                                                                                                              ttACAGCTGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAgaga                                                                                                     <  2:617290/300‑1 (MQ=255)
                                                                                                                                                                                                     ggTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCag                                                                                              <  2:296285/301‑1 (MQ=255)
                                                                                                                                                                                                      gTGCGTCAACATCGCCACAGGTATTGATGGGAAATCGATTATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCaga                                                                                             <  2:339069/301‑1 (MQ=255)
                                                                                                                                                                                                                   gCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACg                                                                                >  2:364257/1‑301 (MQ=255)
                                                                                                                                                                                                                    ccACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAAAGa                                                                               >  2:568591/1‑301 (MQ=255)
                                                                                                                                                                                                                               gATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAACTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGACCACGTAGAGATCGTCAGAGATGCTTTCAAAGATATTGCCgcgc                                                                    >  1:626186/1‑301 (MQ=255)
                                                                                                                                                                                                                                         cGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTAccc                                                          >  2:486242/1‑301 (MQ=255)
                                                                                                                                                                                                                                            cgcttttccgttctaGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAgg                                                       <  1:546185/287‑1 (MQ=255)
                                                                                                                                                                                                                                                          aGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAggagatcggaagagca                                         >  2:546858/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                    ttttCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTgcgc                               <  2:51964/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ttGTTATTGTTCGTCCCGGTGTAATTGTTCGCGGGAAGGTGTTCGGGTATCTGTAAAAATTATGGTTTTAGTTTGAGGATCTGGTGGAAGTCAATGTAAACAGAATTCCAGTCTTTATCGACTTCAACCTGAATCGAAACCGTATCTTTCGGCGTTACCGTCACGCCGTTCCAGCGTTTGTGGTCGTTATCAACATTGATAGTACCGCTGGCATCGATGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTAACGCAGGGATTTTgcgc                               <  1:549709/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                         cacacgacgctcttccgatctgctCCCGGGACGCGTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCt                          <  1:307790/278‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tttGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATCTCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATCTTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTCCTAc                        >  1:28949/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                       cGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAACGATCGTAAGAGATGCGTTCAACGATATTGCCGACAAGGGTTACCCAGGTGTCGTCACGCAGGAATTTTGCGCTTTCTACAGTCGTTAAGCt            >  1:184092/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                              gctCCCGGGACGCGGTCTCGGGAGCGGTAACAATTAAGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTAACTTTCGGCGTCACCGACACTCCGTTCCAGCGTTTGTGTTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGACGGGTTACCCAGGCGACGTCACGAAGGGAATTTGCACTATCTAAAGACGTTACGCTGCCGTTc     >  1:388512/3‑301 (MQ=255)
                                                                                                                                                                                                                                                                                              gctCCCGGGACGCGTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTagatcggaagagcacacatct     >  2:308194/3‑281 (MQ=255)
                                                                                                                                                                                                                                                                                                 cccGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTTCCGTTCGGc  >  2:144054/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                 cccGGGACGCGTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTAAAGTCGTTACACGGCCGTTCGGc  >  2:493684/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                 
CACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGC  >  NC_000913/3168965‑3169554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: