Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,956,973 +A 7.1% coding (848/894 nt) ilvY ← transcriptional activator of ilvC; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,956,9731.A 7.1% 208.0 / 12.2 56coding (848/894 nt)ilvYtranscriptional activator of ilvC; autorepressor
Reads supporting (aligned to +/- strand):  ref base . (30/22);  new base A (2/2);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

CGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGACCAGTCCGG  >  NC_000913/3956675‑3957265
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                    
cgcTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCa                                                                                                                                                                                                                                                                                                    <  2:248465/301‑1 (MQ=255)
   ttCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCtacatg                                                                                                                                                                                                                                                                                                  <  2:228171/301‑6 (MQ=255)
       acAACACGTTGGGTACGTACTGGAACATTTCTTTGTTCCACTAGCGCAGCCATGGCCCCGACGACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGcc                                                                                                                                                                                                                                                                                             <  1:166384/300‑1 (MQ=255)
               ttGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaattg                                                                                                                                                                                                                                                                                     <  2:121617/300‑6 (MQ=255)
               gctctgccgttcgtgAACATTTTTTTGTGCTACTAACGCAGCAATGGCACCGACGACGGGCGCTGACTGGCGGCGTTCGAGCTTGGCGACCATGAACCGTGTGTCGACACCCGGCGGAATGAGCTGGGCGACGGGTGCCACGACGAAACGAATGACCCGGCGTTCAGGTTCTGTGTGGCGGGTGAGGGAGAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAGCGACATTTGTGGTTCGGCAAAATCGTCCAGAATGCCTCAATGAGCGGCTCATGTAGCCTCTTTTTc                                                                                                                                                                                                                                                                                     <  1:370810/287‑1 (MQ=255)
                            ggcaCATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGAGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGGAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGAAGCCGCTTGTTcagaacggaagaga                                                                                                                                                                                                                                                                        >  2:371306/4‑288 (MQ=255)
                            ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGCTTGCCACGACGAAACCAATAACCCGGCGTTCACGTTCTTTTTGGCGGGATAGGGAAAAATGCCTGATAGCGCTTCGCTTCTCAGGCCTACCCGCGCGACAACGCCATTTGTGGTTCGGCAAACTCTTCCAGACTGCCTCAATTAGCGACTCAACTAGCCCCTTTTTCTGCCCacacaccc                                                                                                                                                                                                                                                                        >  1:361887/1‑299 (MQ=255)
                               aCATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTAACCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTACATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCa                                                                                                                                                                                                                                                                     >  2:634452/1‑301 (MQ=255)
                                  tttCTTTGTTCCACTGTCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGcc                                                                                                                                                                                                                                                                  >  2:8891/1‑300 (MQ=255)
                                     ttttGTTTCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGTCGACCATGAACCGGATTTCGAACCCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATGAGCCGCtaattgaggcattctggaagattttgcc                                                                                                                                                                                                                                                               <  2:348455/300‑28 (MQ=255)
                                            ccACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGATTCGCTTATCAGGCCTACCCGCGCGCCAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACCAACGCCCAGCTCAAACGGCgc                                                                                                                                                                                                                                                        >  1:34665/1‑300 (MQ=255)
                                                tATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTtc                                                                                                                                                                                                                                                    <  2:535957/301‑1 (MQ=255)
                                                   cGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCa                                                                                                                                                                                                                                                 <  2:604820/301‑1 (MQ=255)
                                                            ggCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTgcgc                                                                                                                                                                                                                                        >  2:281924/1‑301 (MQ=255)
                                                                ccGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTa                                                                                                                                                                                                                                    <  2:577083/301‑1 (MQ=255)
                                                                         ggggcgTACTGGCGGCGTTGGTACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTGTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAAtcatca                                                                                                                                                                                                                           <  1:106590/298‑1 (MQ=255)
                                                                               tACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCAGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCATTTTCTCATCGCTGAGCTCTAAAATCATCACGCGGt                                                                                                                                                                                                                     >  2:248546/1‑301 (MQ=255)
                                                                                aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGtt                                                                                                                                                                                                                    <  1:255252/301‑1 (MQ=255)
                                                                                aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGAGGtt                                                                                                                                                                                                                    >  2:408219/1‑301 (MQ=255)
                                                                                            cGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCCCCgaacc                                                                                                                                                                                                        <  2:34716/301‑5 (MQ=255)
                                                                                              aatttGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTTGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCgg                                                                                                                                                                                                      <  1:633575/298‑1 (MQ=255)
                                                                                                                cGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGACAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGACTTTTCTGCGCACACACGCCAAGCCCAAACGGCGCTTTCTCATCGCTGCCCACTAAAATCATCACGCGGCTACCCACCAGTCAGGCGCCGTTTTccaccaacc                                                                                                                                                                                    >  1:23360/1‑298 (MQ=255)
                                                                                                                       cGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGTCGCTTTTTCTGCGCCCACACCCCCAGCTCAACCGGCGTTTTCTCATACCTGCGCTCTAAAACAACCACGCAGTTACGCCCCGGTTCGGGGCTGTTTTCCAGCACCCCTTCCgg                                                                                                                                                                             >  1:344880/1‑301 (MQ=255)
                                                                                                                            cccGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGcaaca                                                                                                                                                                        <  2:345343/301‑1 (MQ=255)
                                                                                                                               ggCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACCACGACATGTGTGCTTCGGCAAAATCTTCCAGACTACATCAATTAGCGGCTCATGTAGCCGCTTTTTATGCGCACACACGCCCAGCTCAAACAGCGATTTCTCATCGCTGCGCTCTAAAACAATCACGCAGAGACGCCCCTGTTCAGGTCTGTTTTACAGcaccacccaccgccaacaac                                                                                                                                                                     >  1:434567/1‑287 (MQ=255)
                                                                                                                                                  ctaTTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGc                                                                                                                                                  <  2:169402/299‑1 (MQ=255)
                                                                                                                                                       gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTAccacca                                                                                                                                             >  2:307146/1‑301 (MQ=255)
                                                                                                                                                        ccACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGccaccaa                                                                                                                                            >  2:52512/1‑300 (MQ=255)
                                                                                                                                                         cACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGACACCCACCATc                                                                                                                                           >  2:538825/1‑301 (MQ=255)
                                                                                                                                                           cgacgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACCCCACAGCCGACTGCCACCACCgc                                                                                                                                         >  1:76479/1‑300 (MQ=255)
                                                                                                                                                             acgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATACCACGCCACAGCCGACTGccaccaccacat                                                                                                                                       >  1:548402/1‑295 (MQ=255)
                                                                                                                                                                aaaCCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCCCCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACACTGCCACGCCACAGCCGAGTaccacccacccccacc                                                                                                                                    >  1:421319/1‑291 (MQ=255)
                                                                                                                                                                 aaCCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACACCTTCCGGCAACAAAGCCACGCCACAGCCGAGTGCCACCACCGAAACCac                                                                                                                                   >  1:269780/1‑300 (MQ=255)
                                                                                                                                                                            gggggtgaggtttgttgtgggCGGGTTAGGAAAAAAGTCGTGGTTGCGGTTCGTTTATGTGTCTTACCCGCGCGACAACGTCATTGGTGGTTCGGCAAAATCGTCCCGAATGCCGCAGTTAGCGGCTCATGTGGCCGCGTTTGCTGCGCACACACGCCCAGCTCAAACGGCGTTTGCGCATCGCTGCGCTCTAAGATCAGCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACATTGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGcc                                                                                                                        <  1:334631/282‑1 (MQ=255)
                                                                                                                                                                                     ggTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCAACAGCCGAGTGCCACCATCGAAACCATCGCTTAATGCCCACCAAccc                                                                                                               >  1:203694/1‑300 (MQ=255)
                                                                                                                                                                                             ttGGGGGGTTAGGGAAAAATGCCTGATAGCGCTGCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTaa                                                                                                       <  1:281621/301‑1 (MQ=255)
                                                                                                                                                                                               ggCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTaaaa                                                                                                     >  2:463161/1‑300 (MQ=255)
                                                                                                                                                                                                      tAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTACTGCCCGCCAAACGAGGCGTAAATCAACggg                                                                                              >  1:274582/1‑301 (MQ=255)
                                                                                                                                                                                                        gggAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGAAACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATACACGGGtt                                                                                            >  2:304601/1‑301 (MQ=255)
                                                                                                                                                                                                          gAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTAc                                                                                          >  2:495479/1‑301 (MQ=255)
                                                                                                                                                                                                                     taTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTg                                                                               <  2:203937/300‑1 (MQ=255)
                                                                                                                                                                                                                                 tttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACcac                                                                   <  2:549075/300‑1 (MQ=255)
                                                                                                                                                                                                                                        ggCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCACACGGCGTTTTCTCATCGCTGCGCTCTAAACTCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACCGCCGAGTGCCACCATCGCAACCCTCGCCTCATGCCCGCCAAACGCGGCGTACAACATCCGGTTAACTAATTTACTCCCTCAACACCACAGATCaa                                                            >  1:560265/1‑301 (MQ=255)
                                                                                                                                                                                                                                          tctctctgcgcgACATCGTCTTTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATg                                                          <  2:560979/294‑1 (MQ=255)
                                                                                                                                                                                                                                              cccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCCCCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAAACATCGGGTTACTGATTTTATTACGTCGAACACACAGTTCAATgcggg                                                      >  1:453461/1‑300 (MQ=255)
                                                                                                                                                                                                                                                 gcgtgACAACGTCATTTGTGTTTCGTCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATgcggcggc                                                   <  2:362371/301‑1 (MQ=255)
                                                                                                                                                                                                                                                    ctaCAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTa                                                <  2:454025/299‑1 (MQ=255)
                                                                                                                                                                                                                                                              aTTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTTCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTg                                      >  2:406162/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                    ggTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAACTCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCCCTTCCGGCACCAATGCAACGCCACAGCCGAGTGCCCACATCGATACCATCCATTCATGCCCCCCAACCGTAGCGTAAATCACCGAGTTACTGACTTTACTGCATCGAAAACACACTACAAAGAGGCTGCCTAACGGccacggctcgcc                                >  1:102860/1‑291 (MQ=255)
                                                                                                                                                                                                                                                                       tCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCCCCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCAAAACCACAGTTCAATGCGGCGGCTGACCGGCCCCttaaccgcccc                             >  1:335014/1‑291 (MQ=255)
                                                                                                                                                                                                                                                                        cGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCCCCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCCTAAATCAACGGGTTACTGATTTTATTGCGTCGAAACCACCGTTCAAATGGGCGGCGTCCCGGccccccaaccgcccca                            >  1:334705/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        cGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCata                            <  2:274885/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                               ttCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACg                     <  2:270078/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAAAAAACGGc                   >  2:376124/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                          ggCCTCAATGATCGGGTCTGGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGAc          <  1:306742/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             cagaaaaaGCGGCTACATGAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCATTGACCaa       >  2:580845/8‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  aaaGCGGCTACATGAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGTGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCAAGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAAGGCGGAGGCGTACCGGCCCCTGATCGGCCATAAAAACCGGCACCCGTAACCAGccccg  >  1:266363/3‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                    
CGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGACCAGTCCGG  >  NC_000913/3956675‑3957265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: