Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913699,4330CA12.7% 103.0 / 6.1 42intergenic (‑256/+141)asnB/umpHasparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base C (19/17);  new base A (5/1);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01
Rejected: Not supported by required number of reads on both strands.

ATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCGGCCAGAATATCGGTACGCAGGTTATCGCCGA  >  NC_000913/699151‑699731
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                            
tttccgttcttcAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                                                                                                                                                            <  2:113217/290‑1 (MQ=255)
          ccAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGAGGCGTGAAGGCCTTaccaaaatcggaag                                                                                                                                                                                                                                                                                  >  1:113093/1‑288 (MQ=255)
           cAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACaaaa                                                                                                                                                                                                                                                                               >  2:177496/1‑301 (MQ=255)
                      cacaTAACCTTCTCCGTTAACTTGGTATTTGTTGCTTGTTGTTTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAAtt                                                                                                                                                                                                                                                                    <  1:544374/301‑1 (MQ=255)
                      cacaTAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCACAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGCTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGATGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCAGGCCT‑‑ACAAAAGAAAGAAAAtt                                                                                                                                                                                                                                                                    >  1:592522/1‑301 (MQ=255)
                                                        cTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATACTTTTTAGCGGGTTTTATTGAATGTTTATCTTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGCCGTTAACACCTTATCCGGCCT‑‑ACAAACGAATGAAAATCAAATATATATCAGGACCTGCGTAGGCCtaataaa                                                                                                                                                                                                                                  >  1:364983/1‑300 (MQ=255)
                                                                   ggtttGTTTAAAAAAAGTCCGCAAAGCATGATTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATGGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATc                                                                                                                                                                                                                       <  1:607106/298‑1 (MQ=255)
                                                                       tttttAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCAGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                                                                                                                                                                   <  2:593283/299‑1 (MQ=255)
                                                                           tAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTTGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGAGTAGGCCTGATAAGCGTACCGCACCAGGCCGtt                                                                                                                                                                                                               >  1:68978/1‑301 (MQ=255)
                                                                                aaaTGCCGCAAAGCAGCACTGTGCGCAAGTGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCg                                                                                                                                                                                                          <  1:619398/301‑1 (MQ=255)
                                                                                    gCCGTAAAGCAGGACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCTATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTg                                                                                                                                                                                                      <  1:308937/301‑1 (MQ=255)
                                                                                     ccGCAAAGCAGCACTGTGTGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGt                                                                                                                                                                                                     >  2:312431/1‑301 (MQ=255)
                                                                                                         cAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTcc                                                                                                                                                                                 >  2:593871/1‑301 (MQ=255)
                                                                                                                      ggTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTg                                                                                                                                                                    >  2:417485/1‑301 (MQ=255)
                                                                                                                                  aaaaaCGTAATGGTGATTGTCGTTTGGTGAAAAAAGGTTTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATaa                                                                                                                                                        <  1:495642/301‑1 (MQ=255)
                                                                                                                                               tGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATCTTTTACGGGGGCCAAATTGCTGACCAAGTGCGATTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCCATATATTGCCGGCGCTGCGTCGGCCTGATAAGCGTAGCGCATCAAGCCGTTTGGCGTTTGTCCTCACAGCCAACCACGTCCGCAAACGTGGTTGCTCTTCAGATAACCTCACTTTCAGc                                                                                                                                           >  1:35581/1‑301 (MQ=255)
                                                                                                                                                    gTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAACTGCGTAGGCCAGATAAGCGTAGCGCAACAGGCAGCTTGACGTTTGTCATCAGCACACACCACGACCGCAGAAGTGGTTGCTATTAAGAAAAcctacataacagcaactg                                                                                                                                      >  1:31554/1‑284 (MQ=37)
                                                                                                                                                         ttGGTTAAAAAAGGTCTGGTGTTAGGATATATTTATTGATGAATCGATAAGTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACggg                                                                                                                                 <  1:177299/301‑1 (MQ=255)
                                                                                                                                                           ggTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGACTCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGCTTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCT‑‑ACAAAAGCATGAACATTCACTATATTGCAGGAGCTGCGTCGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCCGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTAAGCGACTGACgggaa                                                                                                                               >  1:339506/1‑299 (MQ=255)
                                                                                                                                                           ggTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTa                                                                                                                               >  2:228867/1‑301 (MQ=255)
                                                                                                                                                              gAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTaaaa                                                                                                                            >  2:588484/1‑300 (MQ=255)
                                                                                                                                                                     ggTTTGGTGTGAGTATTTTTTTATTGATTATTCGTTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTg                                                                                                                     <  1:416922/301‑1 (MQ=255)
                                                                                                                                                                         tGGTGTGAGGATATATTTATTGTTGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGc                                                                                                                 <  2:339969/301‑1 (MQ=255)
                                                                                                                                                                                      tatttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACt                                                                                                    >  2:438690/1‑301 (MQ=255)
                                                                                                                                                                                              gatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGaaaa                                                                                            >  2:253234/1‑298 (MQ=255)
                                                                                                                                                                                                tgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGCAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAACCAACCACGTCCGCAGACGTGGTTGCTATTCCGATAACGTCGATTACAGCGACTGACCGGTAAATCCAGCTGGAACCAAAAAGCATACAGTccaaaccc                                                                                          >  1:109937/1‑294 (MQ=37)
                                                                                                                                                                                                tgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATAtcg                                                                                          >  2:329769/1‑301 (MQ=255)
                                                                                                                                                                                                      taTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCAGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGc                                                                                    <  2:35632/300‑1 (MQ=255)
                                                                                                                                                                                                                       tttATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAg                                                                   <  1:593110/301‑1 (MQ=255)
                                                                                                                                                                                                                             gAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCaa                                                             >  2:249697/1‑300 (MQ=255)
                                                                                                                                                                                                                                      atgttgaCGGGGGCCAAATTGCTGACAAATTGCGATTTGTTCAGGCCGGGTGCGGCGTGAACGCCTTAGCCGGCCT‑‑ACAAAAGCATGAAAATTCAATAGATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGtctc                                                    <  1:228624/295‑1 (MQ=255)
                                                                                                                                                                                                                                       tttttACGGGGGCCAATGTGCGGACAAAGTGCGATTTGTTCATGCCGGAGGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTcc                                                   <  1:587760/300‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGCAAGCACCAGAACCGCACCCAGACCTGCCCgg                                       >  1:20347/1‑301 (MQ=255)
                                                                                                                                                                                                                                                      aagtgtttaCAAAGGGTGTTTTGTTCATGCCGGATGAGGCGTTAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAg                                    <  1:438126/293‑1 (MQ=255)
                                                                                                                                                                                                                                                          gCTGATAAAGTGCTGTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCTGACCTGCCTGGAAGCCgg                                <  1:252966/301‑1 (MQ=255)
                                                                                                                                                                                                                                                           gtgacaattggtgATTTTTTAATGCCGGATGCGGCGTGAACGCATTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCGGc                               <  1:141050/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCGCCCAGaaaa                        >  1:17144/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                        gcttttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCGGCCAGAAAAACgg                    >  2:142940/11‑301 (MQ=255)
                                                                                                                                                                                                                                                                              gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACCCCAAAAAGCACCAGAAACGACACCAACCCTGCCTGGAAGCCGGCCAAAATAACGGTAcacacg            >  1:166854/5‑299 (MQ=38)
                                                                                                                                                                                                                                                                                aggCCGGATAAGGCGTTCACGCCGCATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGAACAAGAATCGACTCCAGACCTGCCTGGAAACCGACCAGAATATCggaaaccaagct          >  1:60026/3‑291 (MQ=38)
                                                                                                                                                                                                                                                                                  gCCGGATAAGGCGTTCACGCCGCATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCGGCCAGAATAACGGTACGCAGGTTaa        >  2:278073/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                        tGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCGGCCAGAATATCGGTACGCAGGTTATCGCCGa  <  2:17195/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                            
ATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCT‑‑ACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCGGCCAGAATATCGGTACGCAGGTTATCGCCGA  >  NC_000913/699151‑699731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑