Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,153,219 G→A 6.5% intergenic (+39/‑81) fabF → / → pabC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,153,2190GA 6.5% 251.0 / 3.3 85intergenic (+39/‑81)fabF/pabC3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (37/41);  new base A (4/2);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAA  >  NC_000913/1152926‑1153518
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                           
ttgtTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCttttt                                                                                                                                                                                                                                                                                                      <  2:412948/300‑1 (MQ=255)
    tGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCtt                                                                                                                                                                                                                                                                                                  <  2:325549/301‑1 (MQ=255)
        cgatctcACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCGGACCttttataagggtg                                                                                                                                                                                                                                                                                              <  1:228159/295‑10 (MQ=255)
              cACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCGGACCttttataagggtgagatcg                                                                                                                                                                                                                                                                                        >  2:228402/1‑286 (MQ=255)
                       tATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCCACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGACGCGCGTCCGGTTTGCGGAATTGAATCCCCTCTGTTTAACTCCTTCGGCTTCCGTGGACCTACTGGTTCTTTTATCTTTTAAAAGCTCTACATTCACATTTTCCACCCTTATAAAAGCTCCGCTTTCCGACCTTTTATCTTAGCTTTTATTCCCACTTGt                                                                                                                                                                                                                                                                               >  1:288444/1‑301 (MQ=255)
                              ggTCACCTGTTAGGGGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAg                                                                                                                                                                                                                                                                        <  1:20663/301‑1 (MQ=255)
                               gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACCCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCCAGAAGCGCGGCCGGTTAGCGGAAAGGAATACACTCTGGGTAAACCCTTCGGCTTCGGGGGCACTAATGGTTCTTTGATCTTTCAAAAGATCTAAATTGTCATTTTCCACCCTTATAACAAGTACGCTTGCGGGCCTTTTTTCTTAGCCTTTATTCCTACTTGTTCCGTAGt                                                                                                                                                                                                                                                                       >  1:642644/1‑301 (MQ=255)
                                gcACGTGTGAGGGGGGTCGGGTGCAGGATAATTGTTCTATTCCATCTGGGCGTGGGGCGATCCGGCTGTTCCGCCAACCATTAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGGACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTg                                                                                                                                                                                                                                                                      <  1:332190/300‑1 (MQ=255)
                                 cagacgtgtgctcttccgatctcAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGa                                                                                                                                                                                                                                                                     <  2:25999/279‑1 (MQ=255)
                                       gtAGGTGCGGCGTGGGGAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGAGTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACAtgc                                                                                                                                                                                                                                                               <  1:105969/300‑1 (MQ=255)
                                           ggggggcggGTGAGTTAGAATGTTTTTTCTCCTTCTGGGGTCTGCGCGTTCAGGCTGTTGTGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACGCCTTCGGCTTCGGTGGCACTAATGGTTCTGTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCGTTTATTCCGACTTGTTCCGTAGTGAACATGCTGcc                                                                                                                                                                                                                                                           <  1:5762/297‑1 (MQ=255)
                                           gTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGcc                                                                                                                                                                                                                                                           <  2:105807/301‑1 (MQ=255)
                                                gcgtGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGCCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACt                                                                                                                                                                                                                                                      <  2:148824/301‑1 (MQ=255)
                                                    gTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACa                                                                                                                                                                                                                                                  >  2:162340/1‑301 (MQ=255)
                                                       cAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAAGAtcggaagagcgtcgtgtag                                                                                                                                                                                                                                               >  1:25948/1‑283 (MQ=255)
                                                         gTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTACTGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtct                                                                                                                                                                                                                                             >  2:428652/1‑301 (MQ=255)
                                                         gTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACAATAACAATtct                                                                                                                                                                                                                                             >  1:304598/1‑301 (MQ=255)
                                                           aGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCTTAGTGAACATGCTGCCACACTAACAATTctct                                                                                                                                                                                                                                           >  1:236882/1‑301 (MQ=255)
                                                             aaTCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCAACACTAACAATTCTCTGa                                                                                                                                                                                                                                         >  2:549669/1‑301 (MQ=255)
                                                              tagattctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGCATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGAt                                                                                                                                                                                                                                        >  1:175099/6‑301 (MQ=255)
                                                                 tatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACAAACAATTCTCTGATaaa                                                                                                                                                                                                                                     >  2:31052/1‑300 (MQ=255)
                                                                  atctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAgg                                                                                                                                                                                                                                    >  1:389969/1‑301 (MQ=255)
                                                                   tctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGa                                                                                                                                                                                                                                   <  2:390483/301‑1 (MQ=255)
                                                                    ttaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAg                                                                                                                                                                                                                                  <  2:160910/300‑1 (MQ=255)
                                                                       cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAACAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGCCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCACTGATAAgaaaccc                                                                                                                                                                                                                               >  1:220981/1‑295 (MQ=255)
                                                                             ccTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGCGCCGGTATGt                                                                                                                                                                                                                         >  1:547238/1‑301 (MQ=255)
                                                                                        gcgATCAGGCTGTTCCGCCAACCATCCACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAAc                                                                                                                                                                                                              <  2:643521/301‑1 (MQ=255)
                                                                                           tcttccgatctCCGCCATCCTTCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGt                                                                                                                                                                                                           <  2:3659/291‑1 (MQ=255)
                                                                                             cAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCa                                                                                                                                                                                                         <  2:410302/301‑1 (MQ=255)
                                                                                              aGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACAGTCAt                                                                                                                                                                                                        >  2:179336/1‑301 (MQ=255)
                                                                                                      ccGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTCGCTTTTCTTCCGACTTGTTCCGTCGTTAACATGCTGCCACACCAACAATTCTCTGCAAAGGCGCCGGTATGTTCTTAATTTACGGtaagtcggaagg                                                                                                                                                                                                >  1:3659/1‑290 (MQ=255)
                                                                                                      ccGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGa                                                                                                                                                                                                >  2:629160/1‑301 (MQ=255)
                                                                                                      ccGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGa                                                                                                                                                                                                >  2:522311/1‑301 (MQ=255)
                                                                                                        gCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGaaa                                                                                                                                                                                              >  2:471287/1‑300 (MQ=255)
                                                                                                         ccAACCATCAACCTGGATAACCCGGATGAAGGTTGCGCTCTGGCTTTCGTACCGCACGAAGCGCGTCCGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTCCGGCTTCGGTGGCACTAACGGTTCTTTGATCCTTAAAAAGATCTAAGTTGTCACTATCCACCCTTATGAAAGGTCCGCGTGCAGGCCTTTTTTCTTGGCTCTGATTCACACTTGTTCCATAGTCAACTTACTGCCACACTAACAATACACTAATAACAAACACGTATGTTActaattacccatcaacaaaagacata                                                                                                                                                                                             >  1:268145/1‑282 (MQ=255)
                                                                                                         ccAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATc                                                                                                                                                                                             <  2:547911/301‑1 (MQ=255)
                                                                                                               aTCAACCTGGGTAACCCGGATGAATGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGc                                                                                                                                                                                       <  1:162189/301‑1 (MQ=255)
                                                                                                                                tgtgccgatctGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCaa                                                                                                                                                                      <  1:567523/291‑1 (MQ=255)
                                                                                                                                ggATGAATGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCaa                                                                                                                                                                      <  2:175296/301‑1 (MQ=255)
                                                                                                                                           gCGTTCTGGTGTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGt                                                                                                                                                           <  1:470686/301‑1 (MQ=255)
                                                                                                                                           gCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCaaagataggaaga                                                                                                                                                           >  2:568237/1‑290 (MQ=255)
                                                                                                                                            cGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAATTTGGTg                                                                                                                                                          >  2:299311/1‑301 (MQ=255)
                                                                                                                                             ggtCTGGTTTTCGTGCCGGACGAGTTGCGTCAGTTTAGCTGAATGGAATTCACTCTGTGTAACTCCTTCTGCTGCGGTGGCACTAATGGTTCTTTGATCTATATAAAGATCTAAGTTGTCAGTTTCCACCCTTATTACAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGa                                                                                                                                                         <  1:434294/299‑1 (MQ=255)
                                                                                                                                                 gtgATTTCGTACCGCACGAAGCGTGTCAGGTTAGCGGAATGGAATACACTCTGCGTAACTCCTTCGGCTGCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGgt                                                                                                                                                     <  1:253797/299‑1 (MQ=255)
                                                                                                                                                  ggATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTTAACATGCTGCCACACTAACAATTCTCTGATACGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGgtt                                                                                                                                                    >  1:295894/1‑301 (MQ=255)
                                                                                                                                                          aaCCGCACGAAGCGCGTCAGGTTTGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTacc                                                                                                                                            <  1:31001/300‑1 (MQ=255)
                                                                                                                                                           aCCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAACCAGGAATCGCTGGCAGTAAGCGAACGGGCAACGCCTTTGGGTGTTGTGTGTTTTtccc                                                                                                                                           >  1:333661/1‑300 (MQ=255)
                                                                                                                                                             ctttccctacacgacgttcttccgatttggaatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTaccacc                                                                                                                                         <  1:247619/274‑1 (MQ=255)
                                                                                                                                                                  ggagtgggTCATGTTAGCGGAATGGAATACACTCTGTGTAACTCTTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCGTAGCTTTTAGTCCGACTTGTTCCGTAGTGAACATGCGGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCag                                                                                                                                    <  1:454816/294‑1 (MQ=255)
                                                                                                                                                                         gTCAGGTTAGCGGAATTGAATACACTCTGTGTAACTCTTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATc                                                                                                                             <  2:111467/301‑1 (MQ=255)
                                                                                                                                                                           cAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCgc                                                                                                                           >  1:59404/1‑300 (MQ=255)
                                                                                                                                                                             ggTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGTTATGTTCTTAATTAACGGTCATAACCAGGAATCGCTGGCAGTAAGAGATCGGCCACCGCAGTTTGGTGCTGGTTGTTTTACCACCGCCAGAGTTATCGAcc                                                                                                                         >  1:412173/1‑300 (MQ=255)
                                                                                                                                                                                tAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTa                                                                                                                      <  2:237148/301‑1 (MQ=255)
                                                                                                                                                                                   gggaatggattACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAg                                                                                                                   <  2:305002/300‑1 (MQ=255)
                                                                                                                                                                                         ggaatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTaccaccagatcggaagagcacacgtctgaactcc                                                                                                             >  2:247886/1‑273 (MQ=255)
                                                                                                                                                                                             tacatttgttgtTACTTCTTCGGCTTCGGTTGGACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGtt                                                                                                         <  1:313316/292‑1 (MQ=255)
                                                                                                                                                                                                acTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGAAGGAATCGCTGGCAGTAAGCGATCGGGCAACGAAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATAGACGGTAAAGTAAGTTTGTTAta                                                                                                      >  2:473741/1‑300 (MQ=255)
                                                                                                                                                                                                   ttgtgTAATTCTTTCGGTTTCGGTGTCACTAATGGTTCTTTGATCTTTAAAAAGATCGAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTGATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGgc                                                                                                   <  2:65578/300‑1 (MQ=255)
                                                                                                                                                                                                             tcttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATTTAAGGTGTCTGTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAg                                                                                         <  1:144189/300‑1 (MQ=255)
                                                                                                                                                                                                             ccTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCaa                                                                                         >  2:215727/1‑300 (MQ=255)
                                                                                                                                                                                                              cttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGATGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCAAGAGTTATCGAAGGTAAAGTCAGTTTGTTATCGGCGCATATCCAga                                                                                        >  2:144315/1‑300 (MQ=255)
                                                                                                                                                                                                                 cggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGAc                                                                                     >  2:130167/1‑301 (MQ=255)
                                                                                                                                                                                                                            tggagttcagacgtgtgctcttccgatctaTCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCt                                                                          <  2:6459/272‑1 (MQ=255)
                                                                                                                                                                                                                              aCTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAATGTCCGCTTGCGGGCCGTTTTTCTTAGCTTTGATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTGCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTg                                                                        <  1:130043/301‑1 (MQ=255)
                                                                                                                                                                                                                                        ttttGTTCTTTAAAAAGATTTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTTCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTtg                                                              <  1:215484/300‑1 (MQ=255)
                                                                                                                                                                                                                                           tGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTtgatg                                                           <  2:296298/301‑1 (MQ=255)
                                                                                                                                                                                                                                            gATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGCACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAAGTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCCATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTAAGTTTGTTAACGGCACATATCCAGCAACTACAGGATGCTGGTCAGCGGtgggtgg                                                          >  1:519015/1‑295 (MQ=255)
                                                                                                                                                                                                                                               cTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGAatt                                                       >  1:565394/1‑301 (MQ=255)
                                                                                                                                                                                                                                                         aTCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCtcgatcggaagagggtcggggagggaaaga                                             >  1:6459/1‑272 (MQ=255)
                                                                                                                                                                                                                                                            tagttttttaTTTTCTACCTTTATAAATGGTCTCGTTCCGTGCCTTGTTTCTTAGCTTTTCTTCCGCCTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCGCTGATAAGGGTCCGGTATGTTCTGAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTg                                          <  1:150353/292‑1 (MQ=255)
                                                                                                                                                                                                                                                                ttttCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCt                                      <  2:519687/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ttttccgttttcccTTATACAAGGTCCGCTTGCGGGCTTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCa                                    <  1:562980/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tttgtcgttttgtaagaaaaaaTGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTTTTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCGGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCa                                    <  1:231611/283‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTAAAGGAAGCTTGTCAGCAGTAGATGATTTCCTGTAACTTCTGGCCTCAg                                   >  1:107644/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                        ttCCTCCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAAAATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGa                              <  2:221224/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cctcccTTTTAAAAGGTCCGCTTGCGGGCTTTTTTTCTTCGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGTTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAAc                            <  1:179139/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                             ctaagaaaaaaGGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCtaaagatagaaaga                         >  2:231854/8‑288 (MQ=255)
                                                                                                                                                                                                                                                                             cccTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCaagatcggaaga                         >  2:563694/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                              ccTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCATCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCCGCTTAAACAaga                        >  2:341194/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                ttATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAgaga                      >  2:542594/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                 gaaaaaaGGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTAAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAgagaa                     >  1:445675/4‑300 (MQ=255)
                                                                                                                                                                                                                                                                                  ttaAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATg                    <  2:59472/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   taAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGa                   <  2:566108/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     aaaGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTAAGCTTGAACAAGAGATGaaa                 >  2:417453/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                        ggtctgttttggggCCTTTTTTTTTTGTTTTTATTCCGACTTGGTCCGTAGTGACAATGCTGCCACACTAACAATTTGCTGAGAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACg              <  1:521639/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                 gCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGgcagca     >  2:577301/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                    ggCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGaa  >  2:587304/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                           
GTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGATGGTTGTTTTACCACCGCCAGAGTTATCGACGGTAAAGTCAGTTTGTTATCGGCGCATATCCAGCGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAA  >  NC_000913/1152926‑1153518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: