Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,272,538 A→T 7.7% D11V (GAT→GTT)  chaC → cation transport regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,272,5380AT 7.7% 143.7 / 3.2 52D11V (GAT→GTT) chaCcation transport regulator
Reads supporting (aligned to +/- strand):  ref base A (26/21);  new base T (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

TCGGCGTGATGACGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTA  >  NC_000913/1272241‑1272832
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      
gcggggggatgaCGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTg                                                                                                                                                                                                                                                                                                     <  1:363117/294‑1 (MQ=255)
  ggCGTGATGACGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAACGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGCTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGGAAGGTGATAAAGAGAGAACTCTTAATGAATGCCGATTGTa                                                                                                                                                                                                                                                                                                   >  1:101706/1‑301 (MQ=255)
       gatttcGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACg                                                                                                                                                                                                                                                                                              <  2:515567/296‑1 (MQ=255)
           aCGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGTTTTACAATCGGCAt                                                                                                                                                                                                                                                                                          <  2:539181/301‑1 (MQ=255)
           aCGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCaa                                                                                                                                                                                                                                                                                          >  2:322748/1‑300 (MQ=255)
            cGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTTATGAATGCGGATTTGAAAACgataac                                                                                                                                                                                                                                                                                         >  1:514895/1‑296 (MQ=255)
            cGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCAtt                                                                                                                                                                                                                                                                                         >  2:319127/1‑301 (MQ=255)
             cccAGGCGGGAAGCAACCGTTGCTATAGTGGCCTGGGCTGCGTTGTAGCATGAATATGCCAACGGGTATCATGATTAATGGCATCAAAACTCGTCACACCGGTCGCTTAGTTAAAGCTATTCGTGCTGTGTTGCCTTGCAAGTCGTCCGTGGATTGCATATTGTCCCGTTACTGGTTTCCAAATGAGCAGTAACAATGTACGGACGACAGCAACAAGTTGTCTCAGGGAAGTACTCAGTTGCGTAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGCTTGTAAAACGGCAttt                                                                                                                                                                                                                                                                                        <  1:227385/300‑1 (MQ=255)
                 gTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGGGAAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTAGGtt                                                                                                                                                                                                                                                                                    >  1:483237/1‑300 (MQ=255)
                         aaaaCCGTGCTTAAAGTGGCCTTGGCTGCTGTGAAGCATGAATATGCCAAATGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTgaa                                                                                                                                                                                                                                                                            <  1:501744/300‑1 (MQ=255)
                             ccGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTgaagaaa                                                                                                                                                                                                                                                                        >  2:574839/1‑300 (MQ=255)
                                          ggcgtggtgtgttgtgAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTc                                                                                                                                                                                                                                                           <  1:568787/289‑1 (MQ=255)
                                            tggagttcagacgtgtgctcttccgatctaaaGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTcag                                                                                                                                                                                                                                                         <  2:90276/272‑1 (MQ=255)
                                                               tGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCTGAAGTGTCAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGACTTTGGTGCCATTTAAGACTCACACTTATGGTCCGCAGAACACCGGGCGGCtac                                                                                                                                                                                                                                      >  1:25647/1‑299 (MQ=255)
                                                                        cAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCAATCTTATGGTCAGACTAACAACGGGCGGCTTCGCTggcggc                                                                                                                                                                                                                             >  2:301881/1‑301 (MQ=255)
                                                                         aaaGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAACACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTcagagatcggaagagcgtcgtgtagggaaaga                                                                                                                                                                                                                            >  1:90152/1‑272 (MQ=255)
                                                                                       tAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGCCGCTGGCTTGTCg                                                                                                                                                                                                              >  1:511054/1‑301 (MQ=255)
                                                                                                             aaCCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGAACGGTGTgg                                                                                                                                                                                        >  1:279350/1‑301 (MQ=255)
                                                                                                                        tAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTa                                                                                                                                                                             >  2:68337/1‑301 (MQ=255)
                                                                                                                         aGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTaa                                                                                                                                                                            >  2:547874/1‑300 (MQ=255)
                                                                                                                          tttAAAGCTATTCGTGCGGTGTTGCCTTGCAATTGGTCGGTGGATTGCATATTGTACCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATg                                                                                                                                                                           <  1:318723/300‑1 (MQ=255)
                                                                                                                                        ttcGGTTTTGCCTTGCAATTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTgg                                                                                                                                                             <  2:483838/299‑1 (MQ=255)
                                                                                                                                        tGCGGTGTTGCCTTGCCAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTCTGCCGTGGCGGAAGTGTAAGGTGATAACGCGTGCTTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACATGCTGAGGGACCGGTGTGGATCTTCAGCTCTGGCTGGTTgtggttg                                                                                                                                                             >  1:324604/1‑295 (MQ=255)
                                                                                                                                              gtTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCCGGCGGCGCCGCTGGCTTGTCGACCTGATGAGGGCCCGGTGAGGATCTTCGGCTATGGATCATTAAtgttacatccg                                                                                                                                                       >  1:238711/1‑293 (MQ=255)
                                                                                                                                                tGCCTTGCAAGTTGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGTCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGc                                                                                                                                                     <  2:282964/301‑1 (MQ=255)
                                                                                                                                                        aaGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGTCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGCTCTTCGGCTATTGATCGTTGATGTGGAATCCGGCaatcgaat                                                                                                                                             >  2:471409/1‑294 (MQ=255)
                                                                                                                                                               ccGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTAGGCGCAGCATCGTTTATGTTGAATACAGCACTGGacgtacccgc                                                                                                                                      >  1:349467/1‑292 (MQ=255)
                                                                                                                                                                        gCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCaa                                                                                                                             >  2:411018/1‑300 (MQ=255)
                                                                                                                                                                                tCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGCACGGTGTGGATCTTCGGCTATGGACAGTGGATGTGGAATCCGGCACTGGAGATTACACAATCGTGCAACGCTacac                                                                                                                     >  1:459554/1‑301 (MQ=255)
                                                                                                                                                                                    tttAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGt                                                                                                                 <  2:279653/300‑1 (MQ=255)
                                                                                                                                                                                       aGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCAATGGAGTTTACCGAATAGTGCACCGGTACACAGGTTgg                                                                                                              >  2:114197/1‑301 (MQ=255)
                                                                                                                                                                                        gttgTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGa                                                                                                             <  2:25698/298‑1 (MQ=255)
                                                                                                                                                                                          ggTTTCAAAATTGGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTTTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATg                                                                                                           <  1:68247/301‑1 (MQ=255)
                                                                                                                                                                                                         aGTGAAAATGTGCGGAAGAGACCGAAAAGTGGTCGGAGGGAAGTATGCGGGGGCGGAAGTGGAAGGTGATAACGCGTGATTTCTTGATGAAGGGCGATGGTAAAACGGCATTTGGTGCCATTGAAGAATCACTGTTATGGTCAGGAGAACAACGGGCGGCTGCGCGGGCGGCGACGCTGGCGTGGCGACCTGATGAGGGAGCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTg                                                                                            <  1:470808/301‑1 (MQ=255)
                                                                                                                                                                                                                  ttCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCt                                                                                   <  2:355935/300‑1 (MQ=255)
                                                                                                                                                                                                                    ctggAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGTTAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGa                                                                                 <  1:511644/299‑1 (MQ=255)
                                                                                                                                                                                                                      ggAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGaaa                                                                               >  2:512316/1‑299 (MQ=255)
                                                                                                                                                                                                                              ccAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCCCTGGAGTTTACCGAATCGTGCACCGGTAACCTAGTTGGATAGCATCGCGAATTTTACCTGCCCCTGACCGCCgcgcc                                                                       >  1:244244/1‑300 (MQ=255)
                                                                                                                                                                                                                                  aaaGTTGTTGCAGGGAAGTGTGAAGTGGCGGAAGGGTAAGGTGATAACGCGTGATTTCTGGATGAATGCCGATGGTAAAACGGCATTTGGTGCCATTGAAGAATCACGCTGATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCggg                                                                   <  1:574124/301‑1 (MQ=255)
                                                                                                                                                                                                                                  aaaGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCggg                                                                   <  2:511726/301‑1 (MQ=255)
                                                                                                                                                                                                                                         tCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAAGGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTgcgc                                                            <  2:101830/301‑1 (MQ=255)
                                                                                                                                                                                                                                                     aTGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGaa                                                >  2:158366/1‑300 (MQ=255)
                                                                                                                                                                                                                                                        cAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGa                                             <  2:419959/301‑1 (MQ=255)
                                                                                                                                                                                                                                                               ggaggttgatggtggtAACTTGTGATTTTTTGTTGTATGCCGATTGTAAAACGGCGTTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACTCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGc                                      <  1:114073/291‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     tGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGACTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGAGGCTAGCACTGACAGAGGGCGGACGcacc                >  1:597362/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                       aTTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTGCGCTGGCGGCGACACTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCGTCGGCTATGGAGCGGTGATGTGGAATCCGGAACGGGAGTTTACCGAATCGTGCACCGGTACACGGGTTGGAGGGCATCGCGCATGTTGCCGGAGCCGGAACGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGACAGAGGACGGACGaaccac              >  1:449591/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                         tctgcaccaaaTGCCGTTTTACAATCGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAg            <  2:117303/292‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          tCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGcctgtggtg           >  2:54663/1‑293 (MQ=255)
                                                                                                                                                                                                                                                                                            gcaccaaaTGCCGTTTTACAATCGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGACAGAGGGCGGACGACCCACAgaag         >  1:117179/7‑298 (MQ=255)
                                                                                                                                                                                                                                                                                               ccaaaTGCCGTTTTACAATCGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCg      >  2:447244/4‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                  aaTGCGGATTGTAAAACGGCAGTGGGTGCCATGGAAGAATGACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGGCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCt   <  1:158215/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                   aTGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGcctgtggtgcgtccgccc  <  1:54595/301‑18 (MQ=255)
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      
TCGGCGTGATGACGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTA  >  NC_000913/1272241‑1272832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑