Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,272,542 T→A 7.7% C12* (TGT→TGA chaC → cation transport regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,272,5420TA 7.7% 149.0 / 4.0 52C12* (TGT→TGAchaCcation transport regulator
Reads supporting (aligned to +/- strand):  ref base T (26/21);  new base A (2/2);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GGCGTGATGACGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCC  >  NC_000913/1272243‑1272841
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                           
ggCGTGATGACGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAACGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGCTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGGAAGGTGATAAAGAGAGAACTCTTAATGAATGCCGATTGTa                                                                                                                                                                                                                                                                                                            >  1:101706/1‑301 (MQ=255)
     gatttcGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACg                                                                                                                                                                                                                                                                                                       <  2:515567/296‑1 (MQ=255)
         aCGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGTTTTACAATCGGCAt                                                                                                                                                                                                                                                                                                   <  2:539181/301‑1 (MQ=255)
         aCGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCaa                                                                                                                                                                                                                                                                                                   >  2:322748/1‑300 (MQ=255)
          cGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTTATGAATGCGGATTTGAAAACgataac                                                                                                                                                                                                                                                                                                  >  1:514895/1‑296 (MQ=255)
          cGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCAtt                                                                                                                                                                                                                                                                                                  >  2:319127/1‑301 (MQ=255)
           cccAGGCGGGAAGCAACCGTTGCTATAGTGGCCTGGGCTGCGTTGTAGCATGAATATGCCAACGGGTATCATGATTAATGGCATCAAAACTCGTCACACCGGTCGCTTAGTTAAAGCTATTCGTGCTGTGTTGCCTTGCAAGTCGTCCGTGGATTGCATATTGTCCCGTTACTGGTTTCCAAATGAGCAGTAACAATGTACGGACGACAGCAACAAGTTGTCTCAGGGAAGTACTCAGTTGCGTAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGCTTGTAAAACGGCAttt                                                                                                                                                                                                                                                                                                 <  1:227385/300‑1 (MQ=255)
               gTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGGGAAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTAGGtt                                                                                                                                                                                                                                                                                             >  1:483237/1‑300 (MQ=255)
                       aaaaCCGTGCTTAAAGTGGCCTTGGCTGCTGTGAAGCATGAATATGCCAAATGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTgaa                                                                                                                                                                                                                                                                                     <  1:501744/300‑1 (MQ=255)
                           ccGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTgaagaaa                                                                                                                                                                                                                                                                                 >  2:574839/1‑300 (MQ=255)
                                        ggcgtggtgtgttgtgAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTc                                                                                                                                                                                                                                                                    <  1:568787/289‑1 (MQ=255)
                                          tggagttcagacgtgtgctcttccgatctaaaGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTcag                                                                                                                                                                                                                                                                  <  2:90276/272‑1 (MQ=255)
                                                             tGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCTGAAGTGTCAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGACTTTGGTGCCATTTAAGACTCACACTTATGGTCCGCAGAACACCGGGCGGCtac                                                                                                                                                                                                                                               >  1:25647/1‑299 (MQ=255)
                                                                      cAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCAATCTTATGGTCAGACTAACAACGGGCGGCTTCGCTggcggc                                                                                                                                                                                                                                      >  2:301881/1‑301 (MQ=255)
                                                                       aaaGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAACACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTcagagatcggaagagcgtcgtgtagggaaaga                                                                                                                                                                                                                                     >  1:90152/1‑272 (MQ=255)
                                                                                     tAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGCCGCTGGCTTGTCg                                                                                                                                                                                                                       >  1:511054/1‑301 (MQ=255)
                                                                                                           aaCCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGAACGGTGTgg                                                                                                                                                                                                 >  1:279350/1‑301 (MQ=255)
                                                                                                                      tAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTa                                                                                                                                                                                      >  2:68337/1‑301 (MQ=255)
                                                                                                                       aGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTaa                                                                                                                                                                                     >  2:547874/1‑300 (MQ=255)
                                                                                                                        tttAAAGCTATTCGTGCGGTGTTGCCTTGCAATTGGTCGGTGGATTGCATATTGTACCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATg                                                                                                                                                                                    <  1:318723/300‑1 (MQ=255)
                                                                                                                                      ttcGGTTTTGCCTTGCAATTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTgg                                                                                                                                                                      <  2:483838/299‑1 (MQ=255)
                                                                                                                                      tGCGGTGTTGCCTTGCCAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTCTGCCGTGGCGGAAGTGTAAGGTGATAACGCGTGCTTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACATGCTGAGGGACCGGTGTGGATCTTCAGCTCTGGCTGGTTgtggttg                                                                                                                                                                      >  1:324604/1‑295 (MQ=255)
                                                                                                                                            gtTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCCGGCGGCGCCGCTGGCTTGTCGACCTGATGAGGGCCCGGTGAGGATCTTCGGCTATGGATCATTAAtgttacatccg                                                                                                                                                                >  1:238711/1‑293 (MQ=255)
                                                                                                                                              tGCCTTGCAAGTTGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGTCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGc                                                                                                                                                              <  2:282964/301‑1 (MQ=255)
                                                                                                                                                      aaGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGTCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGCTCTTCGGCTATTGATCGTTGATGTGGAATCCGGCaatcgaat                                                                                                                                                      >  2:471409/1‑294 (MQ=255)
                                                                                                                                                             ccGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTAGGCGCAGCATCGTTTATGTTGAATACAGCACTGGacgtacccgc                                                                                                                                               >  1:349467/1‑292 (MQ=255)
                                                                                                                                                                      gCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCaa                                                                                                                                      >  2:411018/1‑300 (MQ=255)
                                                                                                                                                                              tCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGCACGGTGTGGATCTTCGGCTATGGACAGTGGATGTGGAATCCGGCACTGGAGATTACACAATCGTGCAACGCTacac                                                                                                                              >  1:459554/1‑301 (MQ=255)
                                                                                                                                                                                  tttAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGt                                                                                                                          <  2:279653/300‑1 (MQ=255)
                                                                                                                                                                                     aGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCAATGGAGTTTACCGAATAGTGCACCGGTACACAGGTTgg                                                                                                                       >  2:114197/1‑301 (MQ=255)
                                                                                                                                                                                      gttgTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGa                                                                                                                      <  2:25698/298‑1 (MQ=255)
                                                                                                                                                                                        ggTTTCAAAATTGGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTTTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATg                                                                                                                    <  1:68247/301‑1 (MQ=255)
                                                                                                                                                                                                       aGTGAAAATGTGCGGAAGAGACCGAAAAGTGGTCGGAGGGAAGTATGCGGGGGCGGAAGTGGAAGGTGATAACGCGTGATTTCTTGATGAAGGGCGATGGTAAAACGGCATTTGGTGCCATTGAAGAATCACTGTTATGGTCAGGAGAACAACGGGCGGCTGCGCGGGCGGCGACGCTGGCGTGGCGACCTGATGAGGGAGCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTg                                                                                                     <  1:470808/301‑1 (MQ=255)
                                                                                                                                                                                                                ttCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCt                                                                                            <  2:355935/300‑1 (MQ=255)
                                                                                                                                                                                                                  ctggAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGTTAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGa                                                                                          <  1:511644/299‑1 (MQ=255)
                                                                                                                                                                                                                    ggAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGaaa                                                                                        >  2:512316/1‑299 (MQ=255)
                                                                                                                                                                                                                            ccAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCCCTGGAGTTTACCGAATCGTGCACCGGTAACCTAGTTGGATAGCATCGCGAATTTTACCTGCCCCTGACCGCCgcgcc                                                                                >  1:244244/1‑300 (MQ=255)
                                                                                                                                                                                                                                aaaGTTGTTGCAGGGAAGTGTGAAGTGGCGGAAGGGTAAGGTGATAACGCGTGATTTCTGGATGAATGCCGATGGTAAAACGGCATTTGGTGCCATTGAAGAATCACGCTGATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCggg                                                                            <  1:574124/301‑1 (MQ=255)
                                                                                                                                                                                                                                aaaGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCggg                                                                            <  2:511726/301‑1 (MQ=255)
                                                                                                                                                                                                                                       tCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAAGGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTgcgc                                                                     <  2:101830/301‑1 (MQ=255)
                                                                                                                                                                                                                                                   aTGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGaa                                                         >  2:158366/1‑300 (MQ=255)
                                                                                                                                                                                                                                                      cAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGa                                                      <  2:419959/301‑1 (MQ=255)
                                                                                                                                                                                                                                                             ggaggttgatggtggtAACTTGTGATTTTTTGTTGTATGCCGATTGTAAAACGGCGTTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACTCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGc                                               <  1:114073/291‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   tGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGACTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGAGGCTAGCACTGACAGAGGGCGGACGcacc                         >  1:597362/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                     aTTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTGCGCTGGCGGCGACACTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCGTCGGCTATGGAGCGGTGATGTGGAATCCGGAACGGGAGTTTACCGAATCGTGCACCGGTACACGGGTTGGAGGGCATCGCGCATGTTGCCGGAGCCGGAACGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGACAGAGGACGGACGaaccac                       >  1:449591/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                       tctgcaccaaaTGCCGTTTTACAATCGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAg                     <  2:117303/292‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        tCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGcctgtggtg                    >  2:54663/1‑293 (MQ=255)
                                                                                                                                                                                                                                                                                          gcaccaaaTGCCGTTTTACAATCGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGACAGAGGGCGGACGACCCACAgaag                  >  1:117179/7‑298 (MQ=255)
                                                                                                                                                                                                                                                                                             ccaaaTGCCGTTTTACAATCGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCg               >  2:447244/4‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                aaTGCGGATTGTAAAACGGCAGTGGGTGCCATGGAAGAATGACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGGCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCt            <  1:158215/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                 aTGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGcctgtggtgcgtccgccc           <  1:54595/301‑18 (MQ=255)
                                                                                                                                                                                                                                                                                                          gTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTCGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCCCTGAAAGAGGGCGGACGCACCACAGGCATCGCCTATCGACTGcc  >  2:269830/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                           
GGCGTGATGACGCCAGTCGCGAAGAAACCGCGCATAAAGTGGCCTGGGCTGCTGTGAAGCATGAATATGCCAAAGGGGATGATGATAAATGGCATAAAAAATCGTAAAACCGGTCGCTTAGTTAAAGCTATTCGTGCGGTGTTGCCTTGCAAGTGGTCCGTGGATTGCATATTGTCCCGTTAGTGGTTTCAAAATGAGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCGCATTTTGCCTGCGCCTGACCGCCGGGCGCGGGACTGCGCACCAGCCGGGACGGATGCTTGCACTGAAAGAGGGCGGACGCACCACAGGCGTCGCCTATCGACTGCC  >  NC_000913/1272243‑1272841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: