Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,303,658 T→A 5.9% Y254N (TAT→AAT)  oppB → oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,303,6580TA 5.9% 212.7 / 2.6 68Y254N (TAT→AAT) oppBoligopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (29/35);  new base A (2/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

TGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAG  >  NC_000913/1303360‑1303958
                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                            
tGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTCTGCGGCGGATCATTTTACGTCACGCATTCAAAACTGCTCCGTTACCCGTGCCCCCTTATCAGGGCCCCGCATTTATCGGCCTTATTACCGGTTCTCTGGTTATCCACACCATTTAt                                                                                                                                                                                                                                                                                                            >  1:418924/1‑301 (MQ=255)
      taTTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTg                                                                                                                                                                                                                                                                                                      <  2:412212/300‑1 (MQ=255)
        ttttGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATAAATGGtttcga                                                                                                                                                                                                                                                                                                    <  2:522157/301‑4 (MQ=255)
           tcagacgtgtgctcttccgatcttGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATAAATGGtttcgataa                                                                                                                                                                                                                                                                                                 <  2:50104/278‑7 (MQ=255)
            gCATTGGCGGCCGGTCGGTGGCTGGAATGTGGTGTCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATAGCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATGTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCgggg                                                                                                                                                                                                                                                                                                <  1:382814/301‑1 (MQ=255)
               ttGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGAtt                                                                                                                                                                                                                                                                                             >  2:476480/1‑301 (MQ=255)
                gggttggcGGGCGGTGGTTGGATTTGTGTGGCGCTTAAATTCATGATATTGCCGATGGTTGCGTTGTCACTCGCTTAGATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTGCACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAGACCTGCTCTGTTACCCGTGCTCGCTGAGATGGGCCCCGCATTTGTCGGCATTATTACCGGTGCTATGGTTATCGAAGCCATTTATGGTTTGCCGGGGAtgg                                                                                                                                                                                                                                                                                            <  1:476016/294‑3 (MQ=255)
                gggCTGCCGGGCGGTGGCTGGTATGGTGGGGCGTTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTg                                                                                                                                                                                                                                                                                            <  1:475879/300‑1 (MQ=255)
                 ggtggtggggCGGTGGGTGGATTGGTGGGGCGTTTTAATTCATGATTTTGCTGTTGGTGGTGTTGTCACTCTCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTNAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTgg                                                                                                                                                                                                                                                                                           <  1:32135/294‑1 (MQ=255)
                 ggCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCCTTTGTCGGCATTATTACCGGTCCTATGGTTATCGAAACCATTTATGGTTTGACGGGGAGTgg                                                                                                                                                                                                                                                                                           >  1:418736/1‑301 (MQ=255)
                              tGGCTGTAATGTTGGGGTGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGtt                                                                                                                                                                                                                                                                              <  2:419487/301‑1 (MQ=255)
                               ggCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTa                                                                                                                                                                                                                                                                             >  2:9851/1‑301 (MQ=255)
                                gCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTat                                                                                                                                                                                                                                                                            >  1:512844/1‑300 (MQ=255)
                                 ttGGAATTGTGGGGTGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAAt                                                                                                                                                                                                                                                                           <  2:303410/300‑1 (MQ=255)
                                 ctggaatggttttttgcTTAAATTCATTCTATTGCCGATGGTTTCGTTGTCACTCGCTTCTATCGCCCTTCTTTCTCGTATTACCCGTGGCTCTATGATGTAAGTATTACAATCCCCTGTTATTCTTTCTGCCCGGGCGAAAGGGTTACCTATGCTGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAAt                                                                                                                                                                                                                                                                           >  2:388233/16‑301 (MQ=255)
                                  tGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATAAATGGtttcgataaaaaacggaaaaccgtcaggtagg                                                                                                                                                                                                                                                                          >  1:50036/1‑272 (MQ=255)
                                      ttggtggGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGgcgc                                                                                                                                                                                                                                                                      <  2:419299/300‑1 (MQ=255)
                                      aTGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCTGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGgcgc                                                                                                                                                                                                                                                                      >  2:207242/1‑301 (MQ=255)
                                             ggCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACATATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCAAAACCATTTATGGTTTGCCGAGGATTGGAAAAATGTTCGTTAATGGCGCAATGaaa                                                                                                                                                                                                                                                               >  2:333504/1‑300 (MQ=255)
                                                 tttAAATTCATGTTTTTGCCGATGGTGGCGTTGTCATTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTg                                                                                                                                                                                                                                                           <  1:577615/300‑1 (MQ=255)
                                                 tttAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTg                                                                                                                                                                                                                                                           <  2:297980/300‑1 (MQ=255)
                                                           ttatatTGTCGTTTGTGGGGTTGTCACTCGCTTATATTGCCTGTATTGCGCGTATTACCCGTGGTTCGATGATTGAAGTAGTACACTCCAATTGTATTCGGACGGCGCGGGCGAAAGGGGTACCTATGCGGCGGATCATGTTACTGCACGCATGAAGACCTGCTCTGTTACCCGTGCTCTCTGATATGGGCCCCGCATTTGTCGGCATTGTTACCGGTTCTAGGGGTATCGAAACCATTTATGGTTTGCCGGGGATTGGACGACTGTTCGTTAATGGCGCATTGAACCGGGACTATTCCtt                                                                                                                                                                                                                                                 <  1:600796/299‑1 (MQ=255)
                                                           tGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTCTCGAAACCCTTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCTCATTGAACCGTTACTATTccct                                                                                                                                                                                                                                                 >  1:13333/1‑299 (MQ=255)
                                                                      tctctttcGTTCTCACTCGTTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGcc                                                                                                                                                                                                                                      <  2:114493/294‑1 (MQ=255)
                                                                       cggtggCCTCGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCACTTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCCGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATCTGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATCAGACAACTGTTCTTTAATGACGCATTGACACGTTACTATTCCTTAATGTTaacctt                                                                                                                                                                                                                                     >  1:592766/2‑297 (MQ=255)
                                                                                   cACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCATAGTGTTAAGccaaatcggaaaagc                                                                                                                                                                                                                         >  1:114369/1‑288 (MQ=255)
                                                                                                cGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACACGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGCCAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTACTTAGTATTAAGCCTGACCATCCTGATTGGTACTTTAAcc                                                                                                                                                                                                            >  1:229084/1‑301 (MQ=255)
                                                                                                 gccgcttttGCGTGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCGTGCATAAAGGGTTCCCTTTTGGGCGCGTCCTTGTACGTCACGCATGTCCACCTGGTTTCTTCCCCGTGCGCTTTTGTCTCGGCCCCGCATTTGTCGGCATTATGTCCTGTTCTAGGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCa                                                                                                                                                                                                           <  1:387719/294‑1 (MQ=255)
                                                                                                           cgcgTATTACCTGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTaa                                                                                                                                                                                                 <  1:634293/301‑1 (MQ=255)
                                                                                                                      cGTTGCTCTTTGTTTGATGTTTTACCCTCCATTTTTTTTTGTACGGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCGGTTACCCGTGCTCTCTTAGATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCg                                                                                                                                                                                      <  1:9851/301‑1 (MQ=255)
                                                                                                                      cGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCTTTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTct                                                                                                                                                                                      >  1:425911/1‑300 (MQ=255)
                                                                                                                         cactctttccctccacgacgctcttCCGATCTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGAtg                                                                                                                                                                                   <  1:141507/277‑1 (MQ=255)
                                                                                                                                tGATTGAAGTATTACACTCCGATTTTATTCGTACTGCCCGGGCGAAATGGTTACCTATTCTGCGGATCATTTTACGTCACGCATTAAAACCTTCTCTTTGACCCGTGCTCGCTTAGATGGGCCCCGCATTTGTCGGCAGTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCtata                                                                                                                                                                            <  1:333099/301‑1 (MQ=255)
                                                                                                                                  aTTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAAACGTGACTATTCCTTAGTGTTAAGCATGCACATCCTGGTTGGTGCTTTAACCATTTTGTTTAATTCCATTGTCGAAGTGCtataat                                                                                                                                                                          >  1:414082/1‑299 (MQ=255)
                                                                                                                                                cTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGCTGTGCTATATGCGGTTATCGACCCg                                                                                                                                                            >  1:132658/1‑301 (MQ=255)
                                                                                                                                                         tttccgatcTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGt                                                                                                                                                   <  2:163118/293‑1 (MQ=255)
                                                                                                                                                          tttCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGtt                                                                                                                                                  <  2:13333/300‑1 (MQ=255)
                                                                                                                                                                 cTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATGAAAACCTGCTCTGTTACCCGTGATCTCTTATATGGGCCCCGCATTTGTCGGCACTATTACCGGTTCTCTGGTTATCTACACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATACTGGTTGGTGCTTTAACCCTTTTGTTTCATGCCATTGTCGATGTGCTATATGAGGTTATCAACCCGACAATCCGTTACTGATa                                                                                                                                           >  1:360859/1‑301 (MQ=255)
                                                                                                                                                                  tGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATAc                                                                                                                                          <  2:440294/301‑1 (MQ=255)
                                                                                                                                                                   gCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGtaaaacggaag                                                                                                                                         >  1:162966/1‑291 (MQ=255)
                                                                                                                                                                     ccgtgtgtgtgggTTACTTTTGCTGCGGTTCATTTTACGTGACGGTTTAAAGCCTGTGCTGGTACCCGTGCTCTCGTATATGGGCCCCGCATTTGTCTGCATTAGTGCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACGACTGTTCGTTAATGGCGCAGTGAACCGTGACTATTCCTTAGTGTGAAGCCTGACGAGCCTGGTTGGTGCTTTAACCATTTTGTTTATTGCCATTGTCGATGTGCTAGATGCGGTTATCGACCCGAAAATCCGTTACTGATACTgg                                                                                                                                       <  1:572245/291‑1 (MQ=255)
                                                                                                                                                                               gggTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGCTGTGCTATATGCGGTTATCGACCCGAAAATACGTTACTGATACTGGAGCCCGCgat                                                                                                                             >  1:505850/1‑301 (MQ=255)
                                                                                                                                                                                        ttGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTtaag                                                                                                                    <  2:262394/300‑1 (MQ=255)
                                                                                                                                                                                                       agtcGTCTCGCATTAAAAGCTGTTTTGTTACCCGTGCTCTTTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTGATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCga                                                                                                     <  1:206999/298‑1 (MQ=255)
                                                                                                                                                                                                             cACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAACTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCt                                                                                               >  2:569421/1‑301 (MQ=255)
                                                                                                                                                                                                                 cATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGaaa                                                                                           >  2:248566/1‑301 (MQ=255)
                                                                                                                                                                                                                    ttaatccTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGTACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAAt                                                                                        <  2:361343/296‑1 (MQ=255)
                                                                                                                                                                                                                        aCCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCTCTTTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGATCTAGAGCTGATGTTAAGTAAGCACAACAGCGAGAAGCTGGAAAAATTCa                                                                                    >  2:610190/1‑301 (MQ=255)
                                                                                                                                                                                                                         ccTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAg                                                                                   <  2:414645/301‑1 (MQ=255)
                                                                                                                                                                                                                         ccTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCAGTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTGGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAg                                                                                   <  2:506522/301‑1 (MQ=255)
                                                                                                                                                                                                                                     cccGTGTTTTTTTATATGGGCCCCGCATTTGTTGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTGGGTGCTTTAACCATTTTGTGTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGa                                                                       <  1:568707/301‑1 (MQ=255)
                                                                                                                                                                                                                                             ctctTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCCCGCGATGATGTTAAGTAAGAAAAACAGCAAAACGCTGGAAAAATTAAGTGAAAAGCTGAACGTCAAAg                                                               >  1:543282/1‑301 (MQ=255)
                                                                                                                                                                                                                                                cctctttcccttcccgtcgctcttctgatctaCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTGAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGgc                                                            <  1:341295/271‑1 (MQ=255)
                                                                                                                                                                                                                                                 tataTGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAAACGCTGGAAAATTTCAGTGAAAAGCTGGAGGACGAAGGgcg                                                           >  1:173863/1‑301 (MQ=255)
                                                                                                                                                                                                                                                   aagtggCTCCGTAGTTGTTGGGTTTATTATCGGGTTTTGGGTTATAGGAACCATTTATGGTTTGCCGGTGAGTGGACAACTGTTCGTGAATGTGGAATTGAACCGTGACTAGTACTTAGTGTTAAGCCTGACCATCCTGATTGGGGCTTTAACCATTTTGGGTAATGCGATTTTCGATGTTCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCTCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAAATTCAGTGAAAAGCTGGAGGTCGAAGGGCGCa                                                         <  1:509104/297‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAg                                                        <  2:132783/300‑1 (MQ=255)
                                                                                                                                                                                                                                                     tGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGGTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTTAAAAGCTGGAGGTCGAAGGGCGCAga                                                       >  2:319683/1‑300 (MQ=255)
                                                                                                                                                                                                                                                      gggCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGACAATCCGTTACTGATACTGGCGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGCAGAGCGCAGca                                                      >  2:385593/1‑300 (MQ=255)
                                                                                                                                                                                                                                                          cccGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAAACGCTGGAAAATTTCAGTGAAAAGCAGGAAGTCGAAGGGCGCAGCAtgtg                                                  >  1:170563/1‑301 (MQ=255)
                                                                                                                                                                                                                                                             gttcttttCGGCATTTTTTCCGGTTTTATGGTTTTCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCa                                               <  1:599149/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAAGatc                                          >  2:599910/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                               ttcGGTTTTATGGTTATCGAATCCATTTATGGTTTGCCGGGGATTTGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGttt                             <  2:234234/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   tttCTATGGTTATCGAATCCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATg                         <  2:426475/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     tCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCa                       <  2:513516/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           gTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGAGCAGCTTATGACAGGACGCACGTCGACGTTTTATGCATAAccc                 >  1:600798/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                            cccggcaaaCCATAAATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGt                >  2:351944/7‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                tgAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTgcgg            <  2:170760/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                cGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTgcgg            >  2:507123/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                          tATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAg  <  2:601559/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                            
TGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTGCCGATGGTGGCGTTGTCACTCGCTTATATCGCCAGTATTGCGCGTATTACCCGTGGCTCTATGATTGAAGTATTACACTCCAATTTTATTCGTACTGCCCGGGCGAAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCCGAAAATCCGTTACTGATACTGGAGCTCGCGATGATGTTAAGTAAGAAAAACAGCGAGACGCTGGAAAATTTCAGTGAAAAGCTGGAGGTCGAAGGGCGCAGCTTGTGGCAGGACGCACGTCGACGTTTTATGCATAACCGTGCGGCGGTTGCCAG  >  NC_000913/1303360‑1303958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: