| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,355,064 | A→C | 46.6% | L136R (CTC→CGC) | sapB ← | antimicrobial peptide transport ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,355,064 | 0 | A | C | 46.6% | ‑6.2 / 58.1 | 41 | L136R (CTC→CGC) | sapB | antimicrobial peptide transport ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (12/10); new base C (8/11); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01 | |||||||||||
CGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACG > NC_000913/1354774‑1355358 | cGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGACATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACaaaa < 2:317335/301‑1 (MQ=255) ttGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAATGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAAc < 2:534098/300‑1 (MQ=255) ggACGAAAAATGGTGAAGTGCGACAAACTGCGGGTTGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCGGATACGCATCAGGCGGATCACTTCAGTTGTGGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCg < 1:247307/300‑1 (MQ=255) gACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCga > 2:302865/1‑300 (MQ=255) aatatgagtgtgtggcAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCGTGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACGTCAGTTGTTGGCGCAACCGACAGGGTGATAACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCAGGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACGT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGa < 1:492152/286‑1 (MQ=255) ggACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCAGTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGccagccag < 1:137709/300‑1 (MQ=255) ggTCGCCGCCGCTTTTAAATAGTTCTGGTCATACACTTCGATAGTTCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGAtt < 2:623568/301‑1 (MQ=255) cgccgcTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAg < 2:450929/301‑1 (MQ=255) gTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGACCAGTAAGGCGATGGCGTTg > 2:399337/1‑301 (MQ=255) cacTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGAATAAAAAGCCCAATAAGGCGATGGcataaaccaaa > 1:24274/1‑291 (MQ=255) gATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCAAGCCAGAACACCGGGATTGAAAAACACAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCAACttt > 1:487616/1‑300 (MQ=255) aCGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAACACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAATTATCCTCCCACCTATgg > 1:420546/1‑301 (MQ=255) gATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAACCGGGCAACCAACATAGCGTGAGTGAACAAAACAGCGCCAACAGAAACGCCAGCCAGACCACCGGGCTTGAAAAACCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCAACtactgccccgtacacacc > 1:639875/1‑284 (MQ=255) taGTTGTTGGCGCAACCGACAGGGTGATCACTGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAatcat < 2:521858/300‑1 (MQ=255) cGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCca > 2:51303/1‑301 (MQ=255) gACTGGGTGATGACGTGTAATAGCATGTGGGGGTTTGCGATCATGTTTATGTCATCCCGCCATGGGTAGTACGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGGCACTTATGGCGCGTAATCCCGGCAATCATCCCCGTGGGGATgattg < 1:575318/301‑5 (MQ=255) ttCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAgcg < 2:24325/300‑1 (MQ=255) ccgatttgCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAgcg < 2:292729/294‑1 (MQ=255) gCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGAAGCCAACAATccaccaaaacgg > 1:292325/1‑290 (MQ=255) cATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTCATCGGTTTCACTT‑CGTAGAGCAGATCGAACCGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGCACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCACAGAACGGCGATGGCGATGATCAAGTTATACTGCCACTTATGGCGCGTAATCACGGACATCATCCCCACGGGGATGCCAACATTCAGAGCAAAACCCAAGGCg > 1:230287/1‑301 (MQ=255) gcgcTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATACACAGtt > 2:451253/1‑301 (MQ=255) tggtgtttcagcgtttgctttttcgatctgTCCCAAAGCCCGGCATCAATTAACGCCAAACCCGTAATCGGGTTCCCTTCCGTAG‑GCAGATCCAAACCCCCTGAAACGGGCAACCCACCTAGCGTGCGTGAACAAAACCGCGTCAACCGAAGCGCCAGGCAGAACACCGGGATTTAAAAGCCCAGGAAGGCGATGGCGTTGATCAAGTTATCCCGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCa < 2:247091/272‑1 (MQ=255) tGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaaagatcggaagagcacacgtctgaactccagt > 2:301076/1‑271 (MQ=255) atgtCCGGCCTTGTTGTGTCCTAAATCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCGGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGa < 1:302461/298‑1 (MQ=255) aaGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCa < 2:488262/301‑1 (MQ=255) ccttccgatctcaaCGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCg < 1:517094/291‑1 (MQ=255) cAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAAACg > 2:599925/1‑301 (MQ=255) cAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGAATGCACAGTTCCATCATCGCCGGGAAGACCTCTTTTAACTGTTCGCCAATCg > 2:444693/1‑301 (MQ=255) ggCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGt > 2:139831/1‑301 (MQ=255) aatcaatcaACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCGAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTg < 1:599164/300‑1 (MQ=255) aatcaatcaACGCAAAACCGGTAAGCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTg < 1:51235/300‑1 (MQ=255) atcaatcaACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGa < 2:421109/301‑1 (MQ=255) tcaaCGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGtt < 2:460684/301‑1 (MQ=255) caaCGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGACAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTAGGCAATCGGTTGACCggtt > 1:621102/1‑298 (MQ=255) caaCGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGCGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCgagatcggaaga > 2:517766/1‑290 (MQ=255) gtgaaaCCGATTACCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATACACAGATCCATCGTCGCCGAGAAGACCTCTTTTAACTGTTCGGCAAACGGTTCACCGTTAATgt > 1:400571/4‑300 (MQ=255) aGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCAAGTGGATCAGGCCGt > 2:445657/1‑301 (MQ=255) cccTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCaaaaa > 2:376914/1‑300 (MQ=255) caatccTAGCGTGAAGTCACAAAACAGCGTAAACAGAAGTGCCAGCCAGAAAACCGGGAGTGAAAAGCCAAGTAAGGTGATGGCGTTGATCAAGTTAGCCGGCCACTTATGGCGCGTAATCCCGGCAATCATCCCAAAGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGAGCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCcac < 1:444129/297‑1 (MQ=255) accTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAacggaagagcacacgtctga > 2:495698/1‑283 (MQ=255) aGCGTGAGTGAACAAAACAGCGGCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGAcg < 1:376418/301‑1 (MQ=255) | CGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGATCATTTCATCCCGCCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTT‑CGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAACAGAAGCGCCAGCCAGAACACCGGGATTGAAAAGCCCAGTAAGGCGATGGCGTTGATCAAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCGGTTGACCGTTAATGCTGGAAACACCAAAATCCCAGTGGATCAGGCCGTTAAACCAGAACACCCAGGCATTCCACAGTGACG > NC_000913/1354774‑1355358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |