Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,560,192 G→C 7.0% G246G (GGC→GGG)  ddpB ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,560,1920GC 7.0% 175.0 / 3.0 55G246G (GGC→GGG) ddpBD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base C (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

ATTCACCACCTCCACGTCCGATACGCGGATCAATCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACGCCCAGCCCAAGCC  >  NC_000913/1559909‑1560487
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       
tttCACCACCTCCACGTCCGATACGCGGATCAATCCACAAATAGAGCAAAGCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGc                                                                                                                                                                                                                                                                                        <  2:313860/300‑1 (MQ=255)
atTCACCACCTCCACGTCCGATACGCGGATCAATCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGAACAACGCATTGGGTAGCGCATAACATAAAACGAAAAACCCCCCGGGccaccggccggc                                                                                                                                                                                                                                                                                        >  1:460056/1‑290 (MQ=255)
    accactTCCACGTCCGATACGCGGATCAATCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCgg                                                                                                                                                                                                                                                                                    <  2:251/301‑1 (MQ=255)
              cGTCCGATACGCGGATCAATCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGaa                                                                                                                                                                                                                                                                          >  2:374682/1‑301 (MQ=255)
               gccgctcttccgatctaaTCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCGCCCGGGCCAGCGGCTTGCCCGGCTGGTatatcg                                                                                                                                                                                                                                                                         <  1:507195/285‑6 (MQ=255)
               gTCCGATACGCGGATCAATCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGCTCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACaaaa                                                                                                                                                                                                                                                                         >  1:405928/1‑300 (MQ=255)
               gTCCGATACGCGGATCAACCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCGCCCGGGCCAGCGGCTTGCCCGGCTGGTatatcg                                                                                                                                                                                                                                                                         <  2:460620/301‑6 (MQ=255)
                             tCAATCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGAAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCa                                                                                                                                                                                                                                                           <  2:607474/301‑1 (MQ=255)
                               aaTCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCGCCCGGGCCAGCGGCTTGCCCGGCTGGTatatcgagatcggaagagcaca                                                                                                                                                                                                                                                         >  2:507867/1‑280 (MQ=255)
                                     caAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATCTAGTCATCACTCAATTGTTcc                                                                                                                                                                                                                                                   >  1:514589/1‑301 (MQ=255)
                                       aaTAGAGCAAATCCACCTCCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAg                                                                                                                                                                                                                                                 <  2:130391/301‑1 (MQ=255)
                                                    ctccctgccgtctttccggtctgaaGCAAAGGAAACCCCGACGGCAAAGCCCAGCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCgg                                                                                                                                                                                                                                    <  1:485299/278‑1 (MQ=255)
                                                          caGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTgg                                                                                                                                                                                                                              <  2:90510/301‑1 (MQ=255)
                                                               tGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGgcgagc                                                                                                                                                                                                                         <  2:171830/301‑1 (MQ=255)
                                                                          tAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTATTCACTCAATTGTTCCAGCATCGCTGAACGGagatcggaagagcacacgtctg                                                                                                                                                                                                              >  2:485945/1‑280 (MQ=255)
                                                                          aaaGCAAATGAAACCAGGCCGGCAAAGTCCATCTCTGCCGGGAAGTCGAGCGCCTGTATTTATGTTACTACCCATGCCCCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCa                                                                                                                                                                                                              <  1:95732/300‑1 (MQ=255)
                                                                                aaTGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCCAGTgaa                                                                                                                                                                                                        >  1:299947/1‑301 (MQ=255)
                                                                                     aaCCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTgaacgaac                                                                                                                                                                                                   <  2:406491/301‑1 (MQ=255)
                                                                                           gacgGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGc                                                                                                                                                                                             <  2:300351/301‑1 (MQ=255)
                                                                                               gCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACCATTCCCAAGTGAACGAACGCCAGCGttt                                                                                                                                                                                         >  1:124996/1‑300 (MQ=255)
                                                                                               gCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAACGTTa                                                                                                                                                                                         >  2:230496/1‑301 (MQ=255)
                                                                                                      ccATCACTGCCGGGAAGTCGAGCGCCTTTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCgg                                                                                                                                                                                  <  2:325866/301‑1 (MQ=255)
                                                                                                               cggggAGGTGGAGGGGCTGGTTTGATGTTACTACCCATGCACCCATTCCTGTCCAGGCAAAAACGGTTTCGTTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAagatgag                                                                                                                                                                         <  1:606010/299‑1 (MQ=255)
                                                                                                                                             ctacCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCGCGGGCGGGACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCTGGTAAGATGAGAAGTTACAACGCATAGCAGAAGACATCAca                                                                                                                                           >  2:267476/1‑300 (MQ=255)
                                                                                                                                                      cACCTATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGAACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTc                                                                                                                                  <  2:222301/301‑1 (MQ=255)
                                                                                                                                                                 ggCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTACGATGAGATGTTGCAACGCATTGAAGAAAACTTCACCGTTGCCTTCAAGCAACGCac                                                                                                                       >  1:617005/1‑300 (MQ=255)
                                                                                                                                                                 ggCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGACCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGAGACAATTACCAGGTGAACGAACGCCAGCGATAATGCAGGAAAGATGAGAGATTGACACGCACAGAACAAGAAGTAAACGATGCCTTCAAGCAgcgccc                                                                                                                       >  1:484429/1‑299 (MQ=255)
                                                                                                                                                                      ggCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAga                                                                                                                  >  2:119903/1‑300 (MQ=255)
                                                                                                                                                                       gCAAATACGGTTTCGTTTAGCACTGCGCCATACAACAATTCGCCCAGCGCCAAACCCAAGACGGTAATCGATGGGAGCAACGCTGTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATGTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCa                                                                                                                 <  1:374186/301‑1 (MQ=255)
                                                                                                                                                                       gCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCa                                                                                                                 >  2:370462/1‑301 (MQ=255)
                                                                                                                                                                           aaaCGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATa                                                                                                             >  2:579383/1‑301 (MQ=255)
                                                                                                                                                                                        aaGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGAGGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACg                                                                                                <  1:230253/300‑1 (MQ=255)
                                                                                                                                                                                          gCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAACAGCGCATAGAGCAGAAAAAAACCGGTAACGTg                                                                                              >  1:224546/1‑301 (MQ=255)
                                                                                                                                                                                                  ccATACAACAAATCGCCCAGCGCCAAACCCAATACTGTAATCGATGGGATCAACGCATTGGTTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTtggtg                                                                                      <  2:485030/301‑1 (MQ=255)
                                                                                                                                                                                                         acaaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATccag                                                                               >  2:247204/1‑301 (MQ=255)
                                                                                                                                                                                                                       gcCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACAAACGCCAGCGTTAATGACGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTAAAGCAGCGCATCAAGCAGATAAAATACTGTAACGTACGTTAGTTGATCCAGCCAATCATCAAAcc                                                                 >  2:532395/1‑301 (MQ=255)
                                                                                                                                                                                                                         cAAACACACTACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGACTGTAGTCTTCAATCAATTGTTCCAGCATAGCTGAGCGGATATGGCGCGCGACAATTCCCCGGTGAACGAACGCCAGCGTTAATGCAGGAAAGATGAGATGTTGCAACGCATTGAAGAAGCCTTCACAGGTGACTTCAAGCAGCGACTAGAGCAGCTAAAAACCGGTAACATGAGTGTGTGGACACAgacagacacaccaccat                                                               >  1:110655/1‑292 (MQ=255)
                                                                                                                                                                                                                          aaaCCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGAAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGAACCAGCCAGTCATCAAGCCTTc                                                              >  2:64450/1‑301 (MQ=255)
                                                                                                                                                                                                                          aaaCCAAATACGGTAATCGATGGGATCAACGCATTGGGTTGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTc                                                              <  1:119779/301‑1 (MQ=255)
                                                                                                                                                                                                                            aCCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTccg                                                            <  2:125120/301‑1 (MQ=255)
                                                                                                                                                                                                                               cAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGAACCAACCAGTCAACAAGCCTTccgccc                                                         >  1:10173/1‑300 (MQ=255)
                                                                                                                                                                                                                                    ggggaaTCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCggg                                                    <  1:246937/297‑1 (MQ=255)
                                                                                                                                                                                                                                    cGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCggg                                                    <  2:10173/301‑1 (MQ=255)
                                                                                                                                                                                                                                    cGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATAGACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCggg                                                    <  2:251077/301‑1 (MQ=255)
                                                                                                                                                                                                                                             ggggtgtcactgctttggtgagtgtataacataaACCGATATCCCTGCCGGGCAAGCCGTTGGCCCGTGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTg                                           <  1:369966/276‑1 (MQ=255)
                                                                                                                                                                                                                                              tGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGc                                          <  2:617863/301‑1 (MQ=255)
                                                                                                                                                                                                                                                 gATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTGGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGAGCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGCATTTGAAGa                                       >  1:256343/1‑301 (MQ=255)
                                                                                                                                                                                                                                                   tCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTCCCTTCAAGCAGCGCATCGAGCAGAGAAAAGCCGGTAACGTGCGTTGGTGGATCCCGCCAGGCCTCAAGCCTCCCCCCCCCGGGAAGAATTGGCAGaca                                     >  1:352159/1‑299 (MQ=255)
                                                                                                                                                                                                                                                      gctcttccgatctcgcATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGAcc                                  <  1:72031/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                   cgcATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCagatcgaaagagc                     >  2:72121/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         aaaaaaaaggtgttaCCCGCCGGGCAAGCCGCGGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACg               <  1:578667/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGACCGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCCGCGCCTCGAGCAGATAAAAGACGGTAACGTGCGTTGGTGGCACAAGCCAGTCAACCAGCCTTCAGCCGCCGGGAAGAATTTGCAGATGCACACAAAACAACCCCATAACgcc             >  1:328724/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                           ataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACgcc             <  2:224789/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCAAAATGAAgcc             >  2:42551/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                      taCCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACGCCCAGCCCAAGca  >  2:95490/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       
ATTCACCACCTCCACGTCCGATACGCGGATCAATCCACAAATAGAGCAAATCCACCACCAGGTTGACCAGCACATAAGCAAATGAAACCACGACGGCAAAGCCCATCACTGCCGGGAAGTCGAGCGCCTGTATTGATGTTACTACCCATGCACCCATTCCGGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACGCCCAGCCCAAGCC  >  NC_000913/1559909‑1560487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑