| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,681,622 | T→A | 13.9% | L216Q (CTG→CAG) | folM → | dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,681,622 | 0 | T | A | 13.9% | 109.0 / 9.4 | 43 | L216Q (CTG→CAG) | folM | dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base T (20/17); new base A (2/4); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
ATGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGT > NC_000913/1681328‑1681922 | aTGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGa < 1:472796/301‑1 (MQ=255) cTGGTAAGATTACTGCTTGGTCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTAATCGACCAGGTCGAtcact < 1:167679/301‑1 (MQ=255) tttcagacgtgtgctcttccgatctcgccgcCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTAATCGACCAGGTCGAtcacttctt < 2:26240/276‑4 (MQ=255) aaaTATTACTGCGTGGGCACTGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTAATCGACCAGGTCGAtcacttctt < 2:530539/301‑4 (MQ=255) aaGATTACTGCGTGGGCACGGTCACGCCGCCTGCGATATCAGTCACTTTACCGATTATGTGGGGGAGTGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTAATCGACCAGGTCGAtcacttcttt < 1:407001/301‑5 (MQ=255) gaCGGACACGCCGCCAGGGATATCATTCACTTTACGGAGTATGTGGGGGAGCGCGGTAGCGAAAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCa < 1:396339/300‑1 (MQ=255) cgccgcCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTAATCGACCAGGTAGAtcacttcttaaatcggaaaagcgtcggggaaggg > 1:26189/1‑273 (MQ=255) aggCGATATCATTCACTTTACCGATGATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTAGGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTc < 1:105410/299‑1 (MQ=255) tttattatTCACTTTACCGATTGTGTGGTGGGGCGCGGTAGCGACAAACATTTTGCGTATGCTGCAAGCAAAGCGGCACTGGTTAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCAc < 1:339968/299‑1 (MQ=255) tatCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGATTTGTCACAGGACGCAGTTTCCCaaaa > 2:340431/1‑298 (MQ=255) tctcTTTACTGATTGTGTGGTGGGGGGCGGTAGCGACAAACATTTTGCTTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGc < 1:112846/298‑1 (MQ=255) aCCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCGAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtcgtc > 2:299075/1‑301 (MQ=255) gtggtgGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTCATGAACTTGATGATGCCGCATCTCTACCACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGCCCTGGTCGATTACTTACTTACCAGTTGCTTTGTCCCCGGACGCAGTTTCCCCCTTTATGGCGATCGTCCTCTCCGtt > 1:556552/1‑301 (MQ=255) gggggtgggcggGGTAGCGACAAACATATTGTGTATGCTGAAAGCAAAGCGGCACTGGATTATATGACCCGCTCGTTTGCCCGCATGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGtt < 1:165435/293‑1 (MQ=255) tggtggAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTa > 2:165587/1‑301 (MQ=255) aGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGTCTGTCGTCATCTGCGTTAATACAGCTTTAtac > 1:106498/1‑299 (MQ=255) aCAAACATATTGCGTATGTTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑Ca < 2:541294/301‑1 (MQ=255) aaCATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATATGCGTTAATGCAACTTTAACCAA‑Caaat > 1:514219/1‑301 (MQ=255) cACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAGGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAaca < 2:148028/301‑1 (MQ=255) taataTGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACa < 2:123707/301‑1 (MQ=255) gCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGTATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCAAG‑CATATAATCAACACCAATGCGCTTATCCCCCAACACGAAACAAAAACaaaaaa > 1:49440/1‑301 (MQ=255) gCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATACGCTTATCCCCAAACACGCAACAGAACCAACAAAAAcggcgg > 1:477272/1‑301 (MQ=255) gCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAACGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAG‑ACCAACAAAAGCGGCggg > 2:636534/1‑301 (MQ=255) cTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGANAGAAGTGATCGANNNNNNNNATTACNNNNNNNNCAGTTGCTTNNNCACCGGACGCANNNNNNNACTTGATGGAGNNNNNNNTCTACNNNNNNACA‑CATTATNCNNACATATAATACAAACCCAACCGCATATCCCACAACACACCACAAAACCAACaaaaacccacgcacaacgac > 1:131616/1‑285 (MQ=255) cACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGcatcat > 2:236562/1‑301 (MQ=255) tgaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGCAAAGCGCATCATTCCGGTGaa > 2:517912/1‑301 (MQ=255) tctttccctacactacgctcttccgttctAATGAACATGATGATGCCGAAATTCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAg < 1:209159/273‑1 (MQ=255) ttCTATTGAGCCATGGCGTATCCTGGTTAATGAACATGATGATGCAGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGGGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTGCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGc < 1:236296/301‑1 (MQ=255) tATTGCGCCTTCGCTGTTCCTTTTTAATGAACATGTTGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGGGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGac < 1:298671/301‑1 (MQ=255) aTCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTaa > 2:468237/1‑301 (MQ=255) cGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATAACACAGCGAAACCAGGTaaaa > 2:171567/1‑300 (MQ=255) aaTGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCAAAgagatcggaaaagcacacgtctgaactcca > 2:209402/1‑275 (MQ=255) aaTGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGCTGAAAACCCACAGCGACACCAAGAAAACAAAATAGGAaataa > 1:52404/1‑301 (MQ=255) gAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACGTATGAAAACCGCGCCGGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCg < 1:517240/301‑1 (MQ=255) aaCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTAGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTCCTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGATAATGCAGCCTTATCCAG‑CATATAATCACCACCCATGCGCTTATCCCTCAACACGCAACAGACCCAACAAAAGCGGCGGGCACCACCACCATTCCGTTGCAATACCACAGCAACACCACGTAAACAAAAACGGGacaaaccgc > 1:488363/1‑293 (MQ=255) gAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGCAAACAAAAAAGGGAATAATCAACAAAAAACTaaaaa > 1:123327/1‑301 (MQ=255) aatgtggACAACAGGTGCTGAATAAATCTCTGATGAAATCCGCGTCTGGCGGGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAat < 1:467673/295‑1 (MQ=255) acaGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTgcg < 2:514891/301‑1 (MQ=255) tAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAACGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATAGTTGCGCGTAAGACTTCa > 1:426402/1‑301 (MQ=255) cgcCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCCCTGGcaac > 1:414612/1‑301 (MQ=255) aaaagaaGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATgc < 2:49508/300‑1 (MQ=255) gtaagTAATCGACCAGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATgcgca > 2:392724/3‑300 (MQ=255) gATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGCTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCAATAAgc > 2:592961/1‑301 (MQ=255) gtccTGGTCTATTACTTACTTACCATTTGCTTTGTCACCGGACGCAGTTTCCCACTGGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGCTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCaa < 1:592200/299‑1 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATgcgcttatcgcgcattata < 2:477873/301‑16 (MQ=255) | ATGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAG‑CATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGT > NC_000913/1681328‑1681922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |