Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,870,253 T→C 12.7% intergenic (+15/‑132) yeaH → / → yeaI UPF0229 family protein/putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,870,2530TC12.7% 91.5 / 4.2 37intergenic (+15/‑132)yeaH/yeaIUPF0229 family protein/putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (12/20);  new base C (2/3);  total (14/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCTGCCATCAATAAAATCCAGCACTCACATTGCTCTCCTTTTTATGGTTTCTATGGGTACACAAAAATTAAAAGCT  >  NC_000913/1869953‑1870549
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        
cccGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGt                                                                                                                                                                                                                                                                                                          <  2:581405/301‑1 (MQ=255)
              ttatTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGc                                                                                                                                                                                                                                                                                            <  2:421973/300‑1 (MQ=255)
              atatTTGGGCTGCACAGTCAGCGGATGGCGTGAACTGGGCCTATGACTTTCCGCTTTGCCATGAAATCCTGGCGAAAAAATGATGACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACAGTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAA‑‑ATCAGCCAGGCGATAATGACCTGGCTg                                                                                                                                                                                                                                                                                          <  1:285679/301‑1 (MQ=255)
               aatTTGCGCAGAACAAGCATCGGGCGGCGATAAGTGTGCCGATGACTCGTCGCTTTTCCATGAAATACTTGCGAAAAAATTTGTACCTGTTGTTCGTTATTAAAGCTATATCGAAATTACCCGTCGTGACCATCGAACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTGGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACGGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGCGATAATGACCTGGCt                                                                                                                                                                                                                                                                                           <  1:328185/300‑1 (MQ=255)
                    aCGCTGCACTAGCATCGGTCTGCGATAACTGGGCCGATGACTCTCCGCTTGGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAttt                                                                                                                                                                                                                                                                                      <  2:548939/301‑1 (MQ=255)
                     cGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAtttt                                                                                                                                                                                                                                                                                     >  2:296900/1‑301 (MQ=255)
                        tGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAg                                                                                                                                                                                                                                                                                  <  1:68233/301‑1 (MQ=255)
                            caAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACCGCTAAAGGCTAAAACTTACAGACAGGCCAATATCCCCTGGCTGATTTTTACCttt                                                                                                                                                                                                                                                                              >  1:753/1‑300 (MQ=255)
                              agcagcGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAA‑‑ATCAGCCAGGCGATAATGACCTGGCTGAtagttttagccttta                                                                                                                                                                                                                                                                          <  1:151739/301‑15 (MQ=255)
                                            aTAACTGGTCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCAt                                                                                                                                                                                                                                                              <  2:644274/301‑1 (MQ=255)
                                                     ccGATGCCTTTCCGTTTTGCCATGAATTCCTGGCGAAAAATTTTTTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGAACATCCGCGACCAGGATGATATTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATtacata                                                                                                                                                                                                                                                    <  2:281324/301‑1 (MQ=255)
                                                     ccGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATtacaa                                                                                                                                                                                                                                                     >  2:382809/1‑300 (MQ=255)
                                                      cGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATtacata                                                                                                                                                                                                                                                    <  2:753/301‑1 (MQ=255)
                                                      cGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATtacata                                                                                                                                                                                                                                                    >  1:378835/1‑301 (MQ=255)
                                                        ttGATTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATtacataca                                                                                                                                                                                                                                                  <  2:602653/300‑1 (MQ=255)
                                                             tctcCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGc                                                                                                                                                                                                                                             <  1:382313/301‑1 (MQ=255)
                                                                                      cGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAga                                                                                                                                                                                                                    >  2:313167/1‑300 (MQ=255)
                                                                                            tattattACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAAcc                                                                                                                                                                                                              <  2:616518/300‑1 (MQ=255)
                                                                                                    ccTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGAAACAGCTCAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCCCATTTATTACATACATTAGCTTACAATTCTTTTAAATATGACAGCATAACCttttattt                                                                                                                                                                                                      >  1:325909/1‑296 (MQ=255)
                                                                                                                   taCAGCTATATCGAAATTACCCGTCGTGCACAGCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGAGAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAACATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGaac                                                                                                                                                                                       <  2:262897/301‑2 (MQ=255)
                                                                                                                                  tttACCCGTCTTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaa                                                                                                                                                                        <  2:379331/300‑1 (MQ=255)
                                                                                                                                     ccccggtgtGCACATCAGACATTGGTGCGGGAAGATGAGCATCTGCAATCTACTGTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAAt                                                                                                                                                                     <  1:395672/294‑1 (MQ=255)
                                                                                                                                                      aaCATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTa                                                                                                                                                    <  2:326313/300‑1 (MQ=255)
                                                                                                                                                         aTTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAttt                                                                                                                                                 >  1:577512/1‑301 (MQ=255)
                                                                                                                                                               gCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATgg                                                                                                                                           <  2:142143/301‑1 (MQ=255)
                                                                                                                                                                                      cTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAttttttt                                                                                                                    >  1:178513/1‑300 (MQ=255)
                                                                                                                                                                                         cgtttgACAACTTTGCGAGGCAGCACATACGCGACCAGGAGGATATTGATTCGGGGTTCCGTGAACGGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAAGCGCGTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCg                                                                                                                 <  1:609294/296‑1 (MQ=255)
                                                                                                                                                                                           ttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGtt                                                                                                               >  2:38651/1‑301 (MQ=255)
                                                                                                                                                                                                              agaacaTCCGCGATCAGGATGATATTTTTCCGGTGTTTCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGt                                                                                            <  1:296496/298‑1 (MQ=255)
                                                                                                                                                                                                               agacttCCGGGACCAGGATGATATTTATCCGGTGTTCCGTTAACTTTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTc                                                                                           <  1:54395/296‑1 (MQ=255)
                                                                                                                                                                                                                              ggATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATAtctc                                                                            >  1:241483/1‑301 (MQ=255)
                                                                                                                                                                                                                               aatgttATTTTTCCGGTGTTCCGTGAACTTTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATctct                                                                           <  1:38599/300‑1 (MQ=255)
                                                                                                                                                                                                                                ttgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCTg                                                                          <  2:241750/300‑1 (MQ=255)
                                                                                                                                                                                                                                ttgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCTg                                                                          <  2:178710/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        ggCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCTGCCATCAATAAAATCCAGCACTCACATTGCTCTCCTTTATATGGTTTCTATGGGTaa                  >  2:408297/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  aTCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCTGCCATCAATAAAATCCAGCACTCAAATTGCTCTCCTTTTTATGGTTTCTATGGGTACACAAAAATTa        >  1:513886/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                  aTCAGCCAGGCGATAATGACCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCTGCCATCAATAAAATCCAGCACTCACATTGCTCTCCTTTTTATGGTTTCTATGGGTACACAAAAATTa        >  2:256365/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                        cAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCTGCCATCAATAAAATCCAGCACTCACATTGCTCTCCTTTTTATGGTTTCTATGGGTACACAAAAATTAAAAGCt  >  1:514810/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        
CCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGATTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCTGCCATCAATAAAATCCAGCACTCACATTGCTCTCCTTTTTATGGTTTCTATGGGTACACAAAAATTAAAAGCT  >  NC_000913/1869953‑1870549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: