Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,133,824 C→T 6.3% G610D (GGT→GAT)  wzc ← colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,133,8240CT 6.3% 193.3 / 2.4 64G610D (GGT→GAT) wzccolanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (34/25);  new base T (2/2);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

TAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGA  >  NC_000913/2133524‑2134124
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                            
tAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTGCGGCAAAGCGTACGCTCATCAAAAGATCAGAAGGATTTGGCGGAACCTGAc                                                                                                                                                                                                                                                                                                              >  2:282068/1‑301 (MQ=255)
         tCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGCCAAAGCGTTCGCTCATCAACATTTCAAAAGGATTTGGCGGCACCTCACCCCGCgggc                                                                                                                                                                                                                                                                                                     >  1:556846/1‑300 (MQ=255)
                  gggcTGGCGGGGCGGAAGATGGAGTTCAGAATCACCCCTTTCCCCGGAATACCGTTTTGCGCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGATCCCGCGCGGTCAGGtgcc                                                                                                                                                                                                                                                                                            <  1:427101/299‑4 (MQ=255)
                       ggCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTg                                                                                                                                                                                                                                                                                       >  2:203456/1‑301 (MQ=255)
                                        aGTTCAGAATCACCCCTTTCACCGGATTACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGttt                                                                                                                                                                                                                                                                      <  1:203213/301‑1 (MQ=255)
                                        aGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCAACATTAACGTGGTTACGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTAACCAGTTCGGCAAAGCGATCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGAACGAGCGGGACCAGGTCAAATTAGACAATAGAGATCGAttt                                                                                                                                                                                                                                                                      >  2:587962/1‑301 (MQ=255)
                                           tCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGCCGACCAACAATTGCCGCATCGGTCACTGCAAGAATCGGCGGCGTATCCATCAACACCAGGTCATAGTTTTTACTCGCCAAGTTCACCAGTTCGGCAAAGCGTTCGCTCAACAACAGTTCCGACGGATTTGGCGGCACCTGACAGCGCGGGATCAGGTCAAATATGGCAATAGAGGTCGGTTGaga                                                                                                                                                                                                                                                                   >  2:114601/1‑300 (MQ=255)
                                                    ccccTTTCACCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGgtagt                                                                                                                                                                                                                                                          >  2:505498/1‑301 (MQ=255)
                                                     cccTTTCTCCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGgtagta                                                                                                                                                                                                                                                         <  2:125992/301‑1 (MQ=255)
                                                       ccttcacgccgctcttccgatcttCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAat                                                                                                                                                                                                                                                       <  1:428584/278‑1 (MQ=255)
                                                        tttCTCCGGAATTCCTTTTTGTTCACTGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCTCCCCCATTAACGTGTGTCCGACATGACGACCCACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCATGTCGGCAAAGCGTTCGCTCATCAGCAGTTCAGAAGGATTTGGCTGCACCTTACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAata                                                                                                                                                                                                                                                      <  1:114477/301‑1 (MQ=255)
                                                           cACCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATACCg                                                                                                                                                                                                                                                   >  2:27336/1‑301 (MQ=255)
                                                              gtgtgctcttccgatcTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAAGGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGccc                                                                                                                                                                                                                                                <  2:550779/286‑1 (MQ=255)
                                                                              tCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAatagatcggaagagcacacgtctga                                                                                                                                                                                                                                >  2:429148/1‑279 (MQ=255)
                                                                              tCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCACTGACCAATCAGAAttt                                                                                                                                                                                                                                >  2:152146/1‑301 (MQ=255)
                                                                               cAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTACTATCGaccagcacggacgagcgtcg                                                                                                                                                                                                                               >  1:550106/1‑284 (MQ=255)
                                                                                   gCGGCTCAGGTGTGTTTCCAGTTCTTTTAATGGTTTGACCTCTTAACGCGCGACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTGCACCAGTGCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGAc                                                                                                                                                                                                                           <  1:356397/301‑1 (MQ=255)
                                                                                        tCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATGGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGAAAGGcc                                                                                                                                                                                                                      >  2:220499/1‑301 (MQ=255)
                                                                                                      aCTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGttattaa                                                                                                                                                                                                        >  2:9824/1‑300 (MQ=255)
                                                                                                                 tgtgTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGc                                                                                                                                                                                             >  2:342608/1‑301 (MQ=255)
                                                                                                                   tgtTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAACTc                                                                                                                                                                                           >  2:602112/1‑301 (MQ=255)
                                                                                                                         ccGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCGGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAACTTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAACTCATgggg                                                                                                                                                                                     >  1:305728/1‑300 (MQ=255)
                                                                                                                          cttccgatctgcCACCATTAACGTGGTTCCGACAGGACGACCAACAATTGCCGCATCGGTAACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGgtg                                                                                                                                                                                    <  1:613463/291‑1 (MQ=255)
                                                                                                                                  cggcCACCATTAATGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATACCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCttt                                                                                                                                                                            <  1:27285/299‑1 (MQ=255)
                                                                                                                                    gcCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGgtgagatcggaaa                                                                                                                                                                          >  2:614321/1‑291 (MQ=255)
                                                                                                                                          aTTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCataa                                                                                                                                                                    >  2:157736/1‑300 (MQ=255)
                                                                                                                                            tAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCg                                                                                                                                                                  <  2:205649/301‑1 (MQ=255)
                                                                                                                                             aaCGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCATGGGTGTAGCCTTTACGCATATCga                                                                                                                                                                 >  1:205406/1‑300 (MQ=255)
                                                                                                                                               cGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTAATTAGTGCCCAACAGCCCGTGGGTGTAGCCTTTCCACATAACGCAg                                                                                                                                                               >  1:278775/1‑301 (MQ=255)
                                                                                                                                                     tCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGCAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCCCGTGGGTGTAGCCTTGGCGCATAGCGCAGTCGAta                                                                                                                                                         >  1:433347/1‑300 (MQ=255)
                                                                                                                                                           ccgctttccctccacgctgctcttccgatcTGCCAGACTCGGCGGCGTATCAATCAAAACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGGTCGCTCATCAACAGTGCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCaacaac                                                                                                                                                   <  1:381510/272‑1 (MQ=255)
                                                                                                                                                             gacgacCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAacaacaa                                                                                                                                                 >  2:398392/1‑300 (MQ=255)
                                                                                                                                                              acgacCATCAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACAcg                                                                                                                                                <  1:157585/301‑1 (MQ=255)
                                                                                                                                                                   caacaaTTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCTATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCCCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATcaaaaaaaagaggta                                                                                                                                           >  2:286750/1‑292 (MQ=255)
                                                                                                                                                                           gCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATGAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCt                                                                                                                                   >  2:129758/1‑301 (MQ=255)
                                                                                                                                                                               cATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACACGTTTATTGGTCTGGCt                                                                                                                               <  1:397878/301‑1 (MQ=255)
                                                                                                                                                                                         tGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACACCACGCGTTTATTGGTCTGGCTGATCAccgcc                                                                                                                     >  2:202413/1‑301 (MQ=255)
                                                                                                                                                                                          gCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAacaacagatcggaagagcacacgtctgaactccagt                                                                                                                    >  2:382006/1‑271 (MQ=255)
                                                                                                                                                                                          cccAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTAACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCAccgccg                                                                                                                    <  2:600928/300‑1 (MQ=255)
                                                                                                                                                                                                   ggcgtcGTATCATTCAACTCCAGGTCTTAGTTTTTACGCGCCCAGTTCTCCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTgg                                                                                                           <  1:601351/301‑1 (MQ=255)
                                                                                                                                                                                                        cgTATCAATCAACTCCGGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAg                                                                                                      <  2:306132/301‑1 (MQ=255)
                                                                                                                                                                                                          gagttcagacgtgtgctcttccgatcttACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGAc                                                                                                    <  2:239850/275‑1 (MQ=255)
                                                                                                                                                                                                                      ccAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTAcc                                                                                        >  2:577145/1‑301 (MQ=255)
                                                                                                                                                                                                                        aGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTCATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGACCGTTGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAACAAAAGGGTTTACaaa                                                                                      >  2:579473/1‑301 (MQ=255)
                                                                                                                                                                                                                               aGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTCATTGGTCTGGCTGATCACCACCGCCAGGTTGGCGCAGACAAAGGCTTTACCAATTCACgg                                                                               >  1:545675/1‑301 (MQ=255)
                                                                                                                                                                                                                                 tttttACTCGCCAAGTTCTCCTGTTCGGCAAAGCGTTTGCTCCTCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGGCGGTTTAGCAGCGGGAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGc                                                                             <  1:504826/301‑1 (MQ=255)
                                                                                                                                                                                                                                     tACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACagatcggaagagcgtcgagtagggaaa                                                                         >  1:239583/1‑275 (MQ=255)
                                                                                                                                                                                                                                                   aCCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCCAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTCGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCACACGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCACACAAAGGTTTTACCAATTGACGGGCTAACCCCGGtcatcatac                                                           >  1:298634/1‑299 (MQ=255)
                                                                                                                                                                                                                                                     cAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGACAACAAAGGTTTTACCAATTTACGGGCATACCCCGGCCATCATAAac                                                         >  1:334194/1‑301 (MQ=255)
                                                                                                                                                                                                                                                      atttCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAaca                                                        <  2:433911/299‑1 (MQ=255)
                                                                                                                                                                                                                                                      aGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAAACCCGGTCATCATCAaca                                                        >  1:261089/1‑301 (MQ=255)
                                                                                                                                                                                                                                                         tCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGACTTTACCAATTGACGGGATAACCCCGGTCATCATCAAAACAt                                                     >  2:441233/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                  ggtggggtcgtgaacggtgcggAAGGGTTGGGCGGGACTGGACCGCGGGGGTTCAGGTCAAATTTGGCAATGTAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGGGCCCAACAGCTCGTGGGTGTAGCCTGTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCtgtg                                            <  1:9824/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                   gTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGc                                           >  2:169960/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                      cGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTAATAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCCGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCtc                                        >  1:357261/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                       gCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGc                                       >  2:572379/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                        ttcatcaACAGTTCAGAAGGATTTGGCGGAACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGca                                      <  2:279078/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                          gctaaaccgacctctattgccaaatttgaCCTGATCCCGCGCGGTCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGcaac                                    >  1:101324/29‑299 (MQ=255)
                                                                                                                                                                                                                                                                               aCAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTAATAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATcgc                               <  2:357745/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   ttatgagggATTTGGGGGTACCTGACCGGGCGGGTTCAGGTTAAATTTGGCAATAGAGGTCGGTTTAGCATCGGTAGGAATATCGCCCTGATCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAg                           <  1:164081/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     ctctattgccaaatttgaCCTGATCCCGCGCGGTCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTg                         <  2:101448/284‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          ggATTTGGCGTCACCTGACTGCGCGGGATCAGGTTAAATTTGGCAAGAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAAc                    <  2:320688/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                gggggggccTGACCTCGCGGTATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAGGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTAc              <  1:151995/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                 gcggcACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACg             <  1:220256/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                    tgaCCTGATCCCGCGCGGTCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCaa          >  2:142129/3‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                            ccGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGa  <  1:202170/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                            
TAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTCAAAGCGGCTCAGACTGGTTTCCACTTCTTTCAATGTGTTGACCGCATAACGCGCCACCATTAACGTGGTTCCGACATGACGACCAACAATTGCCGCATCGGTCACTGCCAGAATCGGCGGCGTATCAATCAACACCAGGTCATAGTTTTTACTCGCCCAGTTCACCAGTTCGGCAAAGCGTTCGCTCATCAACAGTTCAGAAGGATTTGGCGGCACCTGACCGCGCGGGATCAGGTCAAATTTGGCAATAGAGGTCGGTTTAGCAGCGGTAGTAATATCGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGA  >  NC_000913/2133524‑2134124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: