Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,248,737	T→A	8.3%	*294Y (TAA→TAT)	yeiE ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,248,737	0	T	A	8.3%	136.1 / 3.6	48	*294Y (TAA→TAT)	yeiE	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (25/19);  new base A (2/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGTAAATGTGACAGCAACAGATAATCGACAATCTCCCGCGTGCC  >  NC_000913/2248438‑2249033                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                         ccGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGTAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGCAGGAGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACtt                                                                                                                                                                                                                                                                                                         >  1:176730/1‑301 (MQ=255)       gAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAACATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATCCACGGTTCTAAGACACCTTCATGATCGCCCAGCGATTATAAGTACAGCAGCCAATCTCTTTTCTGGCGAATGAATGCTGAAGTCGCTGCGTAACGAAATCGAAACAGCACCAGCATATTCATGTACAGCCACTCAAcgcc                                                                                                                                                                                                                                                                                                   >  1:480375/1‑300 (MQ=255)                    cAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCggg                                                                                                                                                                                                                                                                                      >  2:481992/1‑301 (MQ=255)                    cAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCggg                                                                                                                                                                                                                                                                                      <  2:615093/301‑1 (MQ=255)                            gTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCCCGAAATCGACACAGCCCCAGCATGTTCTTTTACAGCAAATTAACGCCGCAAATTTCCGGGATCCCAAt                                                                                                                                                                                                                                                                              >  1:580053/1‑301 (MQ=255)                            gTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCaaa                                                                                                                                                                                                                                                                              >  2:546936/1‑300 (MQ=255)                                  ggACGCGCCTTTAATTCACGGCGTAAGCTCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCa                                                                                                                                                                                                                                                                        <  1:481391/300‑1 (MQ=255)                                                       cGTAAGCCCGGCTCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTgg                                                                                                                                                                                                                                                   <  2:480976/301‑1 (MQ=255)                                                          aaGCCCGGGGTTTTTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGaaa                                                                                                                                                                                                                                                <  1:634656/301‑1 (MQ=255)                                                              ccGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAACGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACACGGCACATTTGCGGGATCGCAATAGTCCAAAAAGCGCCGTAGCCCATTAGAAAAgtg                                                                                                                                                                                                                                            >  1:513932/1‑301 (MQ=255)                                                                ggCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCCCATTTACGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGAGTTGGAAAGGTGtt                                                                                                                                                                                                                                          >  1:592996/1‑301 (MQ=255)                                                                                        aaaaCCATCCAAAAATGGGTTCCATGTTAAAAATGAGGGGCGATTGTAGCAGACGATTGGCAATCCGTCCGGCAGAAATACACGGTTATAAGACACCTTCAGGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcaca                                                                                                                                                                                                                  <  1:590183/300‑1 (MQ=255)                                                                                                 ccgctcttcctatcttCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCa                                                                                                                                                                                                         <  1:538682/286‑1 (MQ=255)                                                                                                 cAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCa                                                                                                                                                                                                         >  2:576057/1‑301 (MQ=255)                                                                                                                tCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACgaaagatcggaagagcaa                                                                                                                                                                                          >  2:539355/1‑284 (MQ=255)                                                                                                                  ttAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGACACAACGTACGCATCAGGCGCGGCAGAggg                                                                                                                                                                                        >  2:512739/1‑301 (MQ=255)                                                                                                                     aaaaTGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGCTCGCCCAGGGATTCTAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAACGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGACAGCGCCGCAGCGCGCTGGAAAGGTGTTTTTGCCGATGATGTATACCCCACCACGTACGCACCAGGCCCGCCACAGGGccc                                                                                                                                                                                     >  1:117773/1‑301 (MQ=255)                                                                                                                      aaaTGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACATAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCg                                                                                                                                                                                    >  2:577582/1‑301 (MQ=255)                                                                                                                      aaaTGAGGCGCGATTGTAGCAAACGATTAGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTAATAATCGCACAGGGATTATAAGTAAAGCAGCAAATCTCTTTTCTGGCGAATGCATTCTGAATGCGCTGCGTCACGAAATCGAAACAGCACCCACATGTTCTTGTACAGCAACATAAAGCGGCACATTTGCGAGATCACAATAGTCCAGAAAGCACCGTAGCGCGTTGAAACAGTGTTTTTGCCGAAGATGTATACGCCACCACTTACGAATAATGCACAGAAGAGGGAcca                                                                                                                                                                                    >  2:577590/1‑300 (MQ=255)                                                                                                                                                      agccTTCCGGCAGAAATACAGGTTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAAGGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGa                                                                                                                                                    <  1:214526/299‑1 (MQ=255)                                                                                                                                                               gCAGAAATACACGGTTATAAGACACCTTCATGTTCGCCCAGGGAGTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACATAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATc                                                                                                                                           <  1:576867/301‑1 (MQ=255)                                                                                                                                                               atggtatttcacGGGTGTAATATACCTTGCTGATAGCTCAGTGTTTAGAAGTATACCAGCACATATATTGTCGTGCGAATGTAGGATTATGGAGTTGCGTCACGAAATCGACCCATGTACAGAAGTGTCTTGTACTGCAACATAACGCTGCATATTTTGTGGATCGCAATAGGCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAAAGTACGCATCAGGCGTGTCAGAGGGATCGCAACGTCACTTAATGTGCCTGCATGCAATTGCTCTTCAATc                                                                                                                                           <  1:576875/292‑1 (MQ=255)                                                                                                                                                                              tataAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCACGACAga                                                                                                                            >  2:518861/1‑300 (MQ=255)                                                                                                                                                                                       ccTTCATGATCGCCCAGGGCTTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGCAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCACTAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACCACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTACCTGCCTGCAATTCAACTTCACACACACGTAGCGACAGGCAACTAAtt                                                                                                                   >  1:372747/1‑301 (MQ=255)                                                                                                                                                                                          tCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTTTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTccc                                                                                                                <  1:256284/301‑1 (MQ=255)                                                                                                                                                                                             tGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTACTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTTGCGACAGGCAACTAATTCCCAAc                                                                                                             <  2:223331/301‑1 (MQ=255)                                                                                                                                                                                                tCGCCCAGGGATGATAAGTAAAGCAGTCAATCTCTTTGCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCa                                                                                                          <  1:546263/301‑1 (MQ=255)                                                                                                                                                                                                   cccAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGa                                                                                                       >  2:72986/1‑301 (MQ=255)                                                                                                                                                                                                         gATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCAcc                                                                                                 >  2:38455/1‑301 (MQ=255)                                                                                                                                                                                                                     agcagcCAATTTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGTCCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGAtt                                                                                     <  2:593757/301‑1 (MQ=255)                                                                                                                                                                                                                      gcagcTAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTg                                                                                    <  2:580777/301‑1 (MQ=255)                                                                                                                                                                                                                              tttgttttttgtggAATTCATGTTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAg                                                                            <  2:337051/288‑1 (MQ=255)                                                                                                                                                                                                                                  ttttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTAc                                                                        <  2:365393/301‑1 (MQ=255)                                                                                                                                                                                                                                     tCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCAAAAGGCAACTAATTCCCAACCCACGACGCCCCCCATGTTTAATTCCCCCGGCGTTCCCtt                                                                     >  1:14175/1‑300 (MQ=255)                                                                                                                                                                                                                                                 ccacgtcgatcttccgatttcGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCa                                                         <  1:70812/281‑1 (MQ=255)                                                                                                                                                                                                                                                 atgcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACATAATTCCATAGCCa                                                         >  2:552813/1‑301 (MQ=255)                                                                                                                                                                                                                                                            gcTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCCCCATCCCAAACTTc                                              >  1:635222/1‑301 (MQ=255)                                                                                                                                                                                                                                                               gCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGt                                           <  2:514604/301‑1 (MQ=255)                                                                                                                                                                                                                                                                cccgaggcttttccgttctcACCTGCATTTTTTTGTACAGAAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGTa                                          <  1:215134/282‑1 (MQ=255)                                                                                                                                                                                                                                                                   cACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCCACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCCCCCTCTCAAACTTCGGTAAAt                                       >  1:397507/1‑301 (MQ=255)                                                                                                                                                                                                                                                                   cACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCACTCTAAAACATCGGTAAAt                                       >  2:571440/1‑301 (MQ=255)                                                                                                                                                                                                                                                                     cGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCaagatcggaagagcacacgtc                                     >  2:70902/1‑281 (MQ=255)                                                                                                                                                                                                                                                                                caGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGTAAATGTGACAGCAACaa                          >  2:243187/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   cACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGTaagatcggaagagcacacgt                       >  2:215377/1‑283 (MQ=255)                                                                                                                                                                                                                                                                                           tGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGTAAATGTGACAGCAAAAGAAAATCGACaa               >  2:424441/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                              tgtttgtcAGCAACTTAACGCGGCAGATTTTCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACTGACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACGTAATGTGCCTGCCTGCAATTGATCTTCAAGCACACGTCGCGACAGGCAACTAATTCCCAACCCATGGCGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGTAAATGTGACAGCAACAGATAATCGACAAtct            <  1:518189/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  gTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCACAAACTTCGGTAAATGTGACAGCAACAGAAAATCCACAAtaccccc        >  1:251515/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                       gCAACTTACCGGGGCTCATTTGCGGGTTTGGAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGGTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTGCACTTAATGTGCCTGCCTGCAATGGATCTTCAAGCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGTAAATGTGACAGCAACAGATAATCGACAATCTCCCGCGTGc   <  1:512067/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGTAAATGTGACAGCAACAGATAATCAACAATCCCCCGCGTgaa  >  1:366860/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                         CCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGGCAACTAATTCCCAACCCATGACGCACCGCATGTTTGATTGCCTCGGAGTTACCTAATTCCATCGCCATCTCAAACTTCGGTAAATGTGACAGCAACAGATAATCGACAATCTCCCGCGTGCC  >  NC_000913/2248438‑2249033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑