Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 248,313 | A→C | 7.3% | intergenic (+179/+45) | rayT → / ← lfhA | RAYT REP element‑mobilizing transposase; TnpA(REP)/pseudogene, flagellar system protein, promoterless fragment; flagellar biosynthesis |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 248,313 | 0 | A | C | 7.3% | 188.0 / 3.6 | 64 | intergenic (+179/+45) | rayT/lfhA | RAYT REP element‑mobilizing transposase; TnpA(REP)/pseudogene, flagellar system protein, promoterless fragment; flagellar biosynthesis |
Reads supporting (aligned to +/- strand): ref base A (30/29); new base C (2/3); total (32/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 |
AAGCAAGTGAGTGATTGGCCATTCTCAACGTTCCATCGCGATGTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACTTTCCCGCCCT > NC_000913/248015‑248605 | aaGCAAGTGAGTGATTGGCCATTCTCAACGTTCCATCGCGATGTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAgg > 2:181417/1‑301 (MQ=255) cAAGTGAGTGATTGGCCATTCTCAACGTTCCATAGCGATGTCGCGCGAGGGTTATATCCCAGCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGGCTTATGATGCCTACAAACTTGTGCAGGATCGGTATGCAGGATAAGGCGTTTAGGCCGCAAACGGACGTAATGCGACACTGCCTGATGATACGACGGCGCCTCTTATCCAACCTAAAAAC‑TTTTACC‑GAACAGCTAGGACGGATAAGACGt > 1:30255/1‑301 (MQ=255) ggtgaTTGGCCATTCTCAACGTTCCATCGCGATGTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACAGATAAGGCGTTCAcgc < 2:630672/300‑1 (MQ=255) gtgaTTGGCCATTCTCAACGTTCCATCGCGATGTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACATTGT‑CCTGAAC‑GGTAGGCCGGATAAGGAGTTCAcgcc > 1:616161/1‑301 (MQ=255) tgaTTGTCTCTTCTCAACGTTCCATCGCGATGTCGCGCGAGGGTTATATGCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCTTTTCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCAcgccg < 2:30306/301‑1 (MQ=255) gatttcagacgtgtgctcttccgttcttCGCGCGTGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATGCGGCGTGAACGCCTTATcc < 2:299564/274‑1 (MQ=38) tcATTCTCAACGTTCCATCGCGATGTCGCGCGATGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGCCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATGCGGCGTGAACGCCTTATCCgt < 2:121157/300‑2 (MQ=255) tttgacggtttcatcgggtgtgtgcgcgTGGTTGATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACGTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGTCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATGCGGCGTGAACGCCTTATCCgtccta < 1:385527/278‑6 (MQ=38) cAACGTTCCATCGCGATGTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATCAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTAACATGCCTCCAACA‑TTGTGCCAGAAC‑GGTAGGACGGATAAGGCGATCACCCCGCACCCCGCAGCtgt > 1:52452/1‑301 (MQ=255) ttCCATCGCGATGTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGAAGTAGTGCAca > 2:591526/1‑300 (MQ=255) tCGCGATGTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGATCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGc < 2:32165/301‑1 (MQ=255) cgATGTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACACAC‑TTGTTCCTGAAC‑GGCAGGACGGATAAGGCGTTCACACCCAAACCGGCAGACATGCACCAACCCTgg > 1:349195/1‑301 (MQ=38) tCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTAACATTTCATAATTGTGCGCAGATGCCTGCTGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTCGGCCGGTGAAGGCGTCTATGCCGCATCCGGCAGTTAAGCGCAGACGCCTGATGCGGCGCTTGCGCGGCTTATCAATCCTACAAAC‑TTGTGCCTCAAC‑GGTAGGACGGACACGGCGTGAACGCCTTATccagatcggaaacacctcgtatcgagaca > 1:299160/1‑274 (MQ=37) ggcgcgTGGGGTATGTGCCATCGGTTTGGCGGGGGTCGTAACTGATTTTAGGGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCGTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACtt < 1:96381/300‑1 (MQ=255) gcgcgAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTa < 2:617019/301‑1 (MQ=255) gggTTTTATCCCTGTGATTGGGCGGGGGACGTAACGGATTTTGGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCaa < 1:632517/301‑1 (MQ=255) tttatgtccctgctgttGGGCGGGGGAGTGAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGGGCGCAGGTGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACg < 1:181220/286‑1 (MQ=255) cccATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTACCATTTCATAATTGTGCGCAGATGCCTGACGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCCGACCCGTAGGCCGGATAAGGCGTTTATGCCGCATTCGGCAGTTATGCGCACACTCCTGCTGCGATGCTGCCGCCTCCTATCATGCCTACAAAC‑TTCTGCCTGCAC‑GGTAGGACGGATAAGGCGTTCACGCCGCCTACGGCATTTGTGCACACCAGCAGTATACTTACATCAACCCCCCCac > 1:398100/1‑300 (MQ=255) gATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCtt > 2:59309/1‑301 (MQ=255) aTTGGGCGGGGGTCTTAACTGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTc < 1:59241/301‑1 (MQ=255) tGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTTTCATTTCGTAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCaa < 2:252268/301‑1 (MQ=255) gCGGGGGACGTAACGTATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTc < 2:627123/301‑1 (MQ=255) gCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGCTCACGCCGCATCCGGCAGTTGTGCACCCATGCTGGATACTTACATCACCACCCCCATAATCCCCAAccc > 1:62973/1‑299 (MQ=255) aCGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCCTGCCTACCAAC‑TTGTGCCTGAAC‑GGTAGGACGGATCAGGCGTTCCAGCCGCATACGGCAGTTAGGCACCAATGCTGGATACTTACATAAACGCCACCATAAACTTCAACGACAGCTcc > 1:552332/1‑300 (MQ=255) aaCGGTTTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCg < 2:383147/301‑1 (MQ=255) cGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCAAAATCTTCAACTCCAGCTCATCCGGc > 2:249102/1‑301 (MQ=255) tttGCCGGGGAGCTAATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTCATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGtt < 2:391710/299‑1 (MQ=255) ttcattttttaatttggggaggATTCCTGATGCGACGCTTGCGCGTCTTATCATGCCTTAAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGAGGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATg < 1:248835/280‑1 (MQ=38) acggtgccagtggCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCCACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGgcgc < 2:282334/289‑1 (MQ=255) gtgCGCAGATGCCTGATGTGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAACCCCGGCGCaa > 2:8195/1‑301 (MQ=255) gtcgcggatggtggatggtaggCTAGCGCGTCTTTTCATGCCTACAAACTTGTGCCTGTTCGGTAGTGCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCaaa < 1:242396/280‑1 (MQ=38) cgcAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCCCGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAACACATGAAGCCCCGGCCCAaacc > 1:116401/1‑300 (MQ=255) cAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAaacaac < 2:349658/301‑1 (MQ=255) aGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAAcc < 2:63041/301‑1 (MQ=255) ttccggtttcGACGCTATCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCATACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTg < 1:305301/292‑1 (MQ=255) cGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCATACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAAAAACCTTgagatcggaa > 2:305705/1‑292 (MQ=255) cTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCaaa > 2:502913/1‑299 (MQ=255) gcgTCTTATCATGCCTACAATCTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAgg < 2:225884/301‑1 (MQ=255) tCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGgcg > 2:134521/1‑301 (MQ=255) ttCATGCCTACAAACTTGTGCCTGTTCTGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACACAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGc < 2:553005/300‑1 (MQ=255) cATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGACAGCAAAGGGCGCAGCTg > 2:372088/1‑301 (MQ=255) ttACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTg < 2:95469/300‑1 (MQ=255) cAAACTTGTGTCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCa < 2:398614/301‑1 (MQ=255) aagttGTGCCGTATCGGGTTGCCGGATAAGGGGTTTATGCCGCATCCGGCATGTATGCGCAGATGCCTGATGCGACGCTGTCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCAcc < 1:590765/298‑1 (MQ=255) tgtgCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACcagca < 2:52520/301‑1 (MQ=255) cGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGACCAGCTGCGGTGGCACCAgcagcaac > 2:234155/1‑299 (MQ=255) ggATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCg > 2:82854/1‑301 (MQ=255) ccGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTcccc > 2:597728/1‑301 (MQ=255) gCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCCATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGGTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCACAACCGGGACTCTCCCacccgcctccacccgccc > 1:124153/1‑289 (MQ=39) ggccgggaggtgtggggggatgtcttgtgtgACGCTGGGGCTTCTTATCATGCCTACAAAC‑TTTGGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCa < 1:8195/271‑1 (MQ=255) gtttattgggaggtgcgttaTGCGAGTCTGGCGTGTTTTATCATGCTTACAAAC‑TTGTGCCGGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCACCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACGTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCGGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTgg < 1:233889/282‑1 (MQ=255) tATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTAATCGGCTCTTTCACCAGTGGCaa > 2:563983/1‑300 (MQ=255) tgataagacgcgccagcgTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTCTGGCATCGTAATCTGGAAca > 2:82038/16‑300 (MQ=255) tGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGCTACTTACATCAACGCCCCCATAATCTTCAACTCCCCCTCATCCGGCACTTCGTTATACGACAGCACATGCATCCCCCGCGCCAACACCCTTGCATACCGCGCCAGCAAAGGGCCCAGATGCGGTGCCACCAGCAGCACCGGGTCTTTCCCCGCCGCTGTCCTCTGCTATTTCACCTGTGCCATCGtcccccggcact > 1:528918/1‑290 (MQ=255) aTGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTgg > 2:624483/1‑301 (MQ=255) tCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGCTACGGCGTTCACGCCGCATCCCGCACTTGTGCACCACTGCTCGATACGTACACCAACGCCCCCATAATCTTCCACTCCAGCTCATCCGGCACTTCTTTATCCACCCGCACACTCATCCCCGGACCAAACAACCTTGCATACCGCACCACCCAAACGCACAGCTGAGGTGGCCCCCGCAGCCCCGGCTCTTTCCCCGCCGCTTTCCTCTCCTCTTCAACCTATGGCATCGTCACATGGCACTGGTTGTCAATATTCggct > 1:551049/1‑299 (MQ=255) gCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCGTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCa < 1:371592/301‑1 (MQ=255) ccTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGccc > 1:543287/1‑299 (MQ=255) ccTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCACCGGCCCTTTCACCTGTGGCATCGTACTCCGGAACTGGTTCAGCATCTTAGGACCGACCGCCACCCAGTccacccccc > 1:232882/1‑293 (MQ=255) tGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACt > 2:141402/1‑301 (MQ=255) tGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACt > 2:592112/1‑301 (MQ=255) ggAAC‑GGTAGGACGGGTAAGGCGTTTCCGCCGCATCCGGCAGTTGGGCACCAATGCTGTATACTTACATCAACGCCCCCATAATCTTCAACGCCAGCTCATCCGGCACTTCGTTATACGACAGAACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACt < 1:82730/300‑1 (MQ=255) gggTTGGACGGATAAGGGTTTCACGCCGCTTCCGGCATGTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACTTTcc < 1:596967/300‑1 (MQ=255) gACGGCTAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCCTAATCTTCAGCTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGCACATATTCGGATCGACCGGCACCCTGTCGAACAACACTTTCccccccc > 1:546009/1‑300 (MQ=255) | AAGCAAGTGAGTGATTGGCCATTCTCAACGTTCCATCGCGATGTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGGGAGCGTATCATTTCATAATTGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAC‑TTGTGCCTGAAC‑GGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAGTTGTGCACCAATGCTGGATACTTACATCAACGCCCCCATAATCTTCAACTCCAGCTCATCCGGCACTTCGTTATACGACAGCACATGCAGCCCCGGCGCAAACAACCTTGCATAACGCGCCAGCAAAGGGCGCAGCTGCGGTGGCACCAGCAGCACCGGGTCTTTCCCCGCCGCTTTCATCTGCTCTTTCACCTGTGGCATCGTACTCTGGAACTGGTTGAGCATATTCGGATCGACCGGCACGCTGTCGAGCATCACTTTCCCGCCCT > NC_000913/248015‑248605 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |