| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,549,616 | C→A | 7.5% | intergenic (+210/+30) | yfeW → / ← yfeX | penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,549,616 | 0 | C | A | 7.5% | 196.4 / 4.7 | 64 | intergenic (+210/+30) | yfeW/yfeX | penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic |
| Reads supporting (aligned to +/- strand): ref base C (30/28); new base A (3/2); total (33/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
AATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGG > NC_000913/2549317‑2549915 | aaTCCCTATGTGTTCGAATGCGGTAGGTTGCCGATTGAAACTTATGGTTTGGGTGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGcc < 1:402356/301‑1 (MQ=255) cgttcttGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTGACTGGc < 2:310779/295‑1 (MQ=255) cAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGGTGAGCAGGTGTATGGGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTGACTGGcc < 2:569296/301‑1 (MQ=255) tGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTAAGGCCAAGCAGTAACATTACTGACTGGcagaacg > 1:310375/1‑295 (MQ=255) cGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa > 2:406901/1‑301 (MQ=255) aaaGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATg < 2:107158/301‑1 (MQ=255) ggTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCa < 2:147324/301‑1 (MQ=255) aaGTTGCCGATTGCAATTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGT‑ATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGaaa < 1:96887/300‑1 (MQ=255) gCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCt > 1:456782/1‑301 (MQ=255) cAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCAATTGGTCAACAATATGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTAACAgcgc > 1:340626/1‑301 (MQ=255) atttttGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCa < 2:639551/296‑1 (MQ=255) tATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCa > 2:171283/1‑301 (MQ=255) gattGGGTAGTTGGTCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACt < 1:55501/299‑1 (MQ=255) gggTAGTTGTTCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTc < 1:171086/301‑1 (MQ=255) ggTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACtagatc > 2:55569/1‑296 (MQ=255) ggTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGGGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATAAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCg > 2:642197/1‑301 (MQ=255) gtgtATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGATTACAGCGCCATCAACTTGTCCAGCAACGGTGCgc > 1:288102/1‑300 (MQ=255) gcggcgTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAaataaa > 2:168090/1‑300 (MQ=255) aaGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCgg < 2:457346/301‑1 (MQ=255) aGTAATACAAAGGGCATTCAGTAATTTTATGGGCTGGTCTGAGTGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACTTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTTCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCgg < 1:535276/301‑1 (MQ=255) gCCATTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACg < 2:341089/301‑1 (MQ=255) cagtAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACaa > 2:138050/1‑300 (MQ=255) ggtgggtggagaGTGCTGGCAAACGCAAAACGTCCTGTGTCGCTACGCTTTTCAGGCCTACGTGGAGCCCACAATTTATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTAc < 1:406338/293‑1 (MQ=255) ctggTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTTCCAAATAATAGCCGCCAGATACCGGTTTGGTGAAACCCAACATCGCATCACGCTTAcc > 1:414180/1‑301 (MQ=255) ttGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCgg < 2:639247/300‑1 (MQ=255) cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACAt > 1:120480/1‑301 (MQ=255) cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCACCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACaa > 2:472302/1‑300 (MQ=255) cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACaa > 2:251791/1‑300 (MQ=255) aaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGc < 2:288504/301‑1 (MQ=255) aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGcc > 2:544810/1‑300 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCACCGGTAACGCCAAACATGCCCAGCAGTtcctgcc > 1:72721/1‑300 (MQ=255) ggttACGCTTATCAGTCCTACGTGGTGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAAt < 1:251524/298‑1 (MQ=255) cgcTATGCTTATCAGGCCTACGTGGAGCCCACAAGATATTGAATTAGCACGATTTCGTAGGCCGGAGAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGAACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTGGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAAt < 1:544137/301‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATAGCATCACGCTTACCATCGGTATCGCCAAACATGCTAAGCAGTTGCTGCTCaaa > 2:76514/1‑300 (MQ=255) aCGCTTATCAGGCTTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTa < 2:120604/301‑1 (MQ=255) cGCTTATCAGGCCTACGTGGTGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTAt < 2:414743/301‑1 (MQ=255) ggggtgCCCACAATATTTTGCATTATCACGGTTTTGTAGGCCGGATAAGGCGTGCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTa < 1:137925/296‑1 (MQ=255) ggAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAgg > 2:57002/1‑301 (MQ=255) tcccgtccctgcgcttttccgatctttttCGTAGGCCGGATAATGCTTTCACGCCGCATCCGGCGTGTACAACTCGCACTTTGTCAACAATCTGAGGCCATTCAGTAATGTTACTGCCTGTCCTTTAATCAATGAAGCAGAAACGCTTACAGCGCCATCAACTTTTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGgcgc < 1:468781/275‑1 (MQ=255) tctcttccgatctaTTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGa < 2:156116/288‑1 (MQ=255) cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGa < 2:72811/301‑1 (MQ=255) tatttGAATTTTCACGGTTTCGTTGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACa < 1:641320/298‑1 (MQ=255) aaTTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCCATGAATCAGAACCGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCCCGCTTACCATCGGTATCGCCAACCATGCTCAGCAGTTGCTGCTCAATGTTCAGCAGACGCGCACAGTAAGCGCCGAAGTACAGAccag > 1:79385/1‑299 (MQ=255) aTTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCGGCTCAATGTTATGCAGACCCGCGCAGTAGGCGCAGAAGAACGGaagagg > 1:155965/1‑296 (MQ=255) ttAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCCCAATGTTATGCAGACGCCCGCAGGAGGCGCCGAAGAACAACCCGTgg > 1:221384/1‑300 (MQ=255) gcccgttttCTTAGGCCGGTTAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTAATGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTg < 1:167893/295‑1 (MQ=255) aTTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGatcggaagagaacacgactgaaa > 2:469382/1‑279 (MQ=255) cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAAACCACGAGTGCACCTGGcc > 1:411400/1‑300 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACTGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTACCACTGGCAGTGcc > 2:446939/1‑301 (MQ=255) tAAGGCGTTCAAGCCTCTTCCGGCGTGTACAACGTGAACTTGGTCAACAATATGAGGCAGGTCAGTAAGGTTACTGCCTGGCCTGTAATCAATGAATCAGAAACGCTTACAGCGCCCTCAACTTGTGCAGGGACGGTGCGAAATAAGAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACgg < 1:622479/301‑1 (MQ=255) aGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCa > 2:68845/1‑301 (MQ=255) cgccgcTTCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGc < 1:68755/301‑1 (MQ=255) tCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATTTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAAACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCaaaaaaa > 2:372299/1‑300 (MQ=255) gCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTc < 2:221627/301‑1 (MQ=255) tACAACGCGCTCTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCTCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGccc > 2:88613/1‑301 (MQ=255) ctctcTTGGTCAACAATCTGAGTCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCACTGACTCAGAACCGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGGCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCa < 2:501126/297‑1 (MQ=255) acaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCACATGGCAGTTCCGTACGGCAAGCTCTGGCGAACAAACTTCAGCCCTTTGCCCTCTTCtttacga > 1:243228/1‑297 (MQ=255) aTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCa > 2:55161/1‑301 (MQ=255) aaGGCCAGGCAGTAACATTACTGACTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAAcgc > 2:421506/2‑301 (MQ=255) gCCAGGCAGTAACATTACTGACTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCggg > 2:156800/1‑301 (MQ=255) cagtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtgaggg > 2:565805/1‑300 (MQ=255) agtAATGTTACTGTCTGGCGTTTTATCAATGAATCAGAAACGGTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACTGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGggtgaggtg < 1:446375/301‑1 (MQ=255) aCTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTc < 2:79475/301‑1 (MQ=255) tGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCAACAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCg > 2:38021/1‑301 (MQ=255) gCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCgg < 2:63143/301‑1 (MQ=255) | AATCCCAATATGTTCGAAAGCGGTCAGTTGCCGATTGCAACTTATGGTTGGGTAGTTGATCAGGTGTATGCGGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCAGCAGTTGCTGCTCAATGTTATGCAGACGCGCGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGG > NC_000913/2549317‑2549915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |