Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,215,696 A→T 6.5% intergenic (+23/+31) ileX → / ← yqjH tRNA‑Ile/putative siderophore interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,215,6960AT 6.5% 177.4 / 3.5 62intergenic (+23/+31)ileX/yqjHtRNA‑Ile/putative siderophore interacting protein
Reads supporting (aligned to +/- strand):  ref base A (27/30);  new base T (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

GATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGT  >  NC_000913/3215406‑3215991
                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       
aaggAAAGCGCAGGGTTGGTATCGTGAAACACGATCCGTAACCGTGTGGCCAAGATGTTCTCAACTATGGTTACTCCGTTCTAAGCTCACCGTGAAAAGTATAACGGATTGAAAATACATGGTTGATAAAGACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAAGCCGCTACCATGGCCCCGTAGCGCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGcc                                                                                                                                                                                                                                                                                               <  1:117386/298‑1 (MQ=255)
 aTGAAAGCTCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGCct                                                                                                                                                                                                                                                                                              <  2:625743/301‑1 (MQ=255)
      aGCTCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGCctttgct                                                                                                                                                                                                                                                                                         <  1:506123/301‑4 (MQ=255)
      aGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttc                                                                                                                                                                                                                                                                                         <  1:227640/301‑1 (MQ=255)
          cAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGCctttgctagaa                                                                                                                                                                                                                                                                                     >  2:506795/1‑294 (MQ=255)
           aGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttcttttt                                                                                                                                                                                                                                                                                    <  2:136971/301‑1 (MQ=255)
                           aaCACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTACGACACGGAGTTACTTTATAATCCGCTACCATGCCCCCTTAGCTCAGTGGTTAGCGCAGGCGACTCATAATCGCTTGATCGCTGGTTCACGTCCAGCAGGGGCCACCAGATATAGCAAACGCTGACGAGAAAGCGTAAGCCTTTTTCTTTTTATATCCCAGTACCttt                                                                                                                                                                                                                                                                    >  1:430379/1‑301 (MQ=255)
                               cGACCCTTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGt                                                                                                                                                                                                                                                                <  2:136604/301‑1 (MQ=255)
                                gACCCGTAACCCTGGGGCCAAAATTTTCTCAACCATATTTACTCCTTTCTAAAATCACCCGGAAAAGTATAACGGATTGAAAATTCATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTg                                                                                                                                                                                                                                                               <  1:147390/301‑1 (MQ=255)
                                        aCCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTaaa                                                                                                                                                                                                                                                       >  2:498517/1‑300 (MQ=255)
                                               aGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGACTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGc                                                                                                                                                                                                                                                >  2:580068/1‑301 (MQ=255)
                                                  aaaaaTATCCTCAACCATTTTTATTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACg                                                                                                                                                                                                                                             <  1:601069/300‑1 (MQ=255)
                                                          ccTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGcc                                                                                                                                                                                                                                     >  1:297001/1‑300 (MQ=255)
                                                           cTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCtg                                                                                                                                                                                                                                    >  2:184089/1‑301 (MQ=255)
                                                             cAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCtgtg                                                                                                                                                                                                                                  <  2:271024/301‑1 (MQ=255)
                                                              ttcagacgtgtgctcttccgatcttCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTgg                                                                                                                                                                                                                                 <  2:186686/277‑1 (MQ=255)
                                                               aCCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCATGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCACTGCACGTACCCCCTGTggg                                                                                                                                                                                                                                >  1:348837/1‑301 (MQ=255)
                                                                           ccTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAACACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATCTAACAGTTACTTTGCGTGCCCGTAAGCCGCTGCACGTACCCGCCGTGGGTCATAATGTTcc                                                                                                                                                                                                                    >  1:489790/1‑301 (MQ=255)
                                                                           ccTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATAGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAACCTTTTTCTTTTTATATAACAGTTAATTTGCGTTACAGTAAGCCACAGCACGTACCCGCAGTAGGCCATAATATTcc                                                                                                                                                                                                                    >  2:235570/1‑301 (MQ=39)
                                                                                  aaCATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACCGCAGGCGCGCGGTAATGGATGGATTGCGACACGGAGTTACTTTATACTCCGCTACCATGGCCCCTTACCTCAGCGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACAAGATATAGCAAAGGCAGACGAGAAATCGTACCCATTTTTCTTATTATATATCATTAACTTTGCGTGCCAGTACTCCCCAGCAAGACCCCGCTGTGGACCAACCTGTCCCGCTGCac                                                                                                                                                                                                             >  1:338649/1‑300 (MQ=255)
                                                                                  aaCATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCACTGCACGTACCCGCTGTGGGTCATACTATTCCGCTTCaa                                                                                                                                                                                                             >  1:16694/1‑301 (MQ=255)
                                                                                      tCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTggcaatcggaagagcgtcgtgtaggg                                                                                                                                                                                                         >  1:186444/1‑277 (MQ=255)
                                                                                          cAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGCCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCCGTTACTTTGCGTGCCAGTAAGCCGCTGCCCGTACCCGCTGTGGGTCATCCTGTTCCGCTTCAAAGcggcgg                                                                                                                                                                                                     >  1:283491/1‑301 (MQ=255)
                                                                                              aaaaGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCCGTGGGTCATACTGGTCCGCTTCAAAGCCGCCGCCTa                                                                                                                                                                                                 >  1:226609/1‑301 (MQ=255)
                                                                                                     taACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGACATCGTCAGCCTTTTTCTTTTTATATATCCGTTACTTTCCGTGCCAGTAACCCGCCCCACGTACCAGCTGTGGGTCATACTATACCGCCTCaacccgcccacttaactcctt                                                                                                                                                                                          >  1:314982/1‑282 (MQ=38)
                                                                                                        cGGATTGAAAATACATTGTTTATAAATACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAAc                                                                                                                                                                                       <  2:226852/301‑1 (MQ=255)
                                                                                                        cGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAAc                                                                                                                                                                                       <  2:396976/301‑1 (MQ=255)
                                                                                                         gatctGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACg                                                                                                                                                                                      <  1:67946/297‑1 (MQ=255)
                                                                                                              gAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGagatc                                                                                                                                                                                 >  2:68036/1‑297 (MQ=255)
                                                                                                                     aaTTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCg                                                                                                                                                                          <  2:16745/300‑1 (MQ=255)
                                                                                                                         ttttATAAAAACAGCAGGCGCGCTGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCgg                                                                                                                                                                      <  2:601906/300‑1 (MQ=255)
                                                                                                                            tataAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTa                                                                                                                                                                   >  2:380370/1‑301 (MQ=255)
                                                                                                                              taAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGCGCAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCCGTGGGGCCTAATGTACCGCTTCAAAGCGGCGGCTTAAATTCTTAACACCTTTACCTTCGCCGATTATc                                                                                                                                                                 >  1:65998/1‑301 (MQ=255)
                                                                                                                               aaaaaCAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATcc                                                                                                                                                                <  2:297405/301‑1 (MQ=255)
                                                                                                                                        gggtgggtggTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGTTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGt                                                                                                                                                       <  1:294232/293‑1 (MQ=255)
                                                                                                                                            gcgcgGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGTCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAatc                                                                                                                                                   <  2:45103/301‑1 (MQ=255)
                                                                                                                                                             tgtgctcttccgatctcTTTATAATCCGCTTCCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCa                                                                                                                                  <  2:263250/285‑1 (MQ=255)
                                                                                                                                                                  acacGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTgc                                                                                                                             >  2:382144/1‑301 (MQ=255)
                                                                                                                                                                            aCTTTTTAGTCCTCTACAAGGGGCCCTTATGTCAGTGGTTAGAGCAGGGGACTCATATTGGCTTGGTCGCTGGTTCAAGGCCAGCAGGGGCGACCAGATATAGCAAAGGCTGAGGAGAAATCGTCAGCCTTTTTCTGTTTATATATCAGTTACTTTGCGTGCCAGTAAGACGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGACTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTGGCATCTGCGCCAGAcgcg                                                                                                                   <  1:579344/301‑1 (MQ=255)
                                                                                                                                                                             cTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGccagaacggaagagcgtc                                                                                                                  >  1:262982/1‑284 (MQ=255)
                                                                                                                                                                                    aTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAACTTCTTACCGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCAACGGCAGGGATTTGCATCTGCCCCaacccaccacccccc                                                                                                           >  1:53184/1‑287 (MQ=255)
                                                                                                                                                                                           ctcATGGCCCTTTATTTAAGTGGTTAGAGCAGTTGATTTATATTTGGTTTGTCGGTGGTTCAAGTCCAGCAGGGGCCACCAGTTATAGCAAAGGCTGACGATAATTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTGTCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCt                                                                                                    <  1:235304/299‑1 (MQ=255)
                                                                                                                                                                                              gtGGCCCCTTAGCTCAGGGGTTAGAGGAGGGGACTCATTATGTCTTGGTGGGTGGTTCAAGGCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCtcat                                                                                                 <  1:405013/300‑1 (MQ=255)
                                                                                                                                                                                                  tccccgactctcttccgatctgCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCAtg                                                                                             <  1:429440/280‑1 (MQ=255)
                                                                                                                                                                                                        ggCTCAGGGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTgccag                                                                                       <  1:497870/300‑1 (MQ=255)
                                                                                                                                                                                                         gCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTgccagc                                                                                      <  1:381648/301‑1 (MQ=255)
                                                                                                                                                                                                         cccgacgctcttccgatctcGACTCATGATCGCTTGGTCGCTGGTTAAAGTCCATCAGGGGCCACCAGATATGGCAAAGGCGGACGAGAAATCGTCAGCCTTTTGCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTgccagc                                                                                      <  1:385315/282‑1 (MQ=255)
                                                                                                                                                                                                              tttccctacacgacgctgtgccgatcttttGGGCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTc                                                                                 <  1:568409/273‑1 (MQ=255)
                                                                                                                                                                                                                ggTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTAACGCCTGCTCAACAAGTGCCAACCATTCGa                                                                               >  2:15994/1‑301 (MQ=255)
                                                                                                                                                                                                                  ttAGCGCAGGTGACTCATTTTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTGGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCATCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGata                                                                             <  2:471562/301‑1 (MQ=255)
                                                                                                                                                                                                                       gCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCAtgagatcggaagagcacacgtct                                                                        >  2:430004/1‑280 (MQ=255)
                                                                                                                                                                                                                          ggCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTACCCATCACGTGCCAGCCATTCGACATTAAAAcc                                                                     >  1:210000/1‑301 (MQ=255)
                                                                                                                                                                                                                            cGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTgccagcagatcggaagagcacaaga                                                                   >  2:385811/1‑282 (MQ=255)
                                                                                                                                                                                                                                         cTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTcagatcggaagagcaaacgtctaaactc                                                      >  2:569123/1‑274 (MQ=255)
                                                                                                                                                                                                                                           tGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCACgaaaa                                                    >  2:297520/1‑298 (MQ=255)
                                                                                                                                                                                                                                             gTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCCGACGCCCATCCACCGCCTGCTCATCATGTTCCAGCCATTCACTAATCACAACATAAAGGTCCACgagaact                                                  >  1:233835/1‑298 (MQ=255)
                                                                                                                                                                                                                                                tcttgTTCAAGTCCAGCAGGGGCCACCAGATATAGCAATGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTGTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATcc                                               <  2:339112/297‑1 (MQ=255)
                                                                                                                                                                                                                                                                        accaGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAAATAAACCTGAAAGGCGTTATCCCGCACCCTa                       >  1:137603/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                              aataGCAAAGGCTTATGTGAAATCGTTAGCCTTTTTTTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAgc                 <  1:297116/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aaagaaaaaGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGTGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCCCCTATTCGATATTACAACCATCAAGGTGCGCCAGAAAATCCTGACAAGCCTTATCCCCCACACAAACCAgc                 >  1:133308/7‑301 (MQ=255)
                                                                                                                                                                                                                                                                                    aaaGGCTGGTGAGAATTCGTCAGCCTTTTTCTTTTTGTATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTa           <  1:183847/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             cGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGt  <  2:210243/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       
GATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAATCATCGGCAGGGATTTGCATCTGCGCCAGACGCGCATCTACCGCCTGCTCATCATGTGCCAGCCATTCGATATTAAAACCATCAAGGTGCGCGAGATAATCCTGACAGGCGTTATCCCGCACGCTAACCAGCGCACTAACTTGCGGT  >  NC_000913/3215406‑3215991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: