Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,263,500	G→A	9.1%	P54L (CCG→CTG)	tdcD ←	propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,500	0	G	A	9.1%	152.3 / 5.1	55	P54L (CCG→CTG)	tdcD	propionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (28/22);  new base A (2/3);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

GGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATA  >  NC_000913/3263206‑3263799                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                            ggATCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGGGCCAGTGGAGAAACGTGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGGGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTg                                                                                                                                                                                                                                                                                                       <  2:3150/301‑1 (MQ=255)        ttgTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGCTCAcc                                                                                                                                                                                                                                                                                                <  2:429198/299‑1 (MQ=255)         cGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGAGAGCCAGCACCGCTGGCTCAcca                                                                                                                                                                                                                                                                                               <  2:392309/301‑2 (MQ=255)          agacgtgtgctcttccgaTCTAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc                                                                                                                                                                                                                                                                                              <  2:355159/284‑1 (MQ=255)                         gTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAACACTGCCGCCGTGAGCGATGCGATTGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATACAATTGCCTTCAATGCACCTTCGTACCTTGAGTGCGCCAGCGATTCTGGCTCTCCCCCATTTACGTATAAGa                                                                                                                                                                                                                                                                               >  1:558157/1‑301 (MQ=255)                          tATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGaa                                                                                                                                                                                                                                                                              <  1:161842/301‑1 (MQ=255)                               aaTACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTccccagatcggaaacgcagcgtggt                                                                                                                                                                                                                                                                         >  1:354675/1‑280 (MQ=255)                                 tACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCAGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCAACTTCGTAGCTGTAGTGAGCCAGCGGTGCTGGCTCTCACCCATTTACGGATAAGAATGCAttt                                                                                                                                                                                                                                                                       >  1:207367/1‑301 (MQ=255)                                 aaCCGCCACCTGAGTTTCGCCTGGAAATAATTGCTGCGCCGATTTAATACCACTTAAATTGGCGTAATTATGGAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACGCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAttt                                                                                                                                                                                                                                                                       <  1:140121/300‑1 (MQ=255)                                       gtgtgctcttcctatctgAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAg                                                                                                                                                                                                                                                                 <  2:552340/284‑1 (MQ=255)                                             ggTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATa                                                                                                                                                                                                                                                           <  2:84764/300‑1 (MQ=255)                                                    cccGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTCAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGAACCTTCGTATCTGTTGTTAGCCAGCGGTGCTGGCTCTCCCCCATTTACGAATACGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                                                                                                                                                                                    >  1:344044/1‑301 (MQ=255)                                                        gAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCACTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAgagatcggaagagcgtcc                                                                                                                                                                                                                                                >  1:551667/1‑284 (MQ=255)                                                                  cgtttgctcttccgatctaCTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACAtt                                                                                                                                                                                                                                      <  2:584208/283‑1 (MQ=255)                                                                   tgcGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATTCACCTTCGTCGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTa                                                                                                                                                                                                                                     >  2:426369/1‑301 (MQ=255)                                                                     tgtgctcttccgatctcTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt                                                                                                                                                                                                                                   <  2:93119/285‑1 (MQ=255)                                                                            tCAATTCCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcac                                                                                                                                                                                                                            <  1:55886/301‑1 (MQ=255)                                                                            tCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAAGAAGGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcac                                                                                                                                                                                                                            <  1:217673/301‑1 (MQ=255)                                                                                  acACTTAAATTTGCGTAATTATGCAGGGGGGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGc                                                                                                                                                                                                                      <  1:498597/300‑1 (MQ=255)                                                                                    aCTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTagaacggaagagcgtcgt                                                                                                                                                                                                                    >  1:583484/1‑284 (MQ=255)                                                                                     cTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATagatcggaaaagcgtc                                                                                                                                                                                                                   >  1:92995/1‑286 (MQ=255)                                                                                               cGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCaa                                                                                                                                                                                                         >  2:516400/1‑300 (MQ=255)                                                                                                 atcttATGCAGGGGTGCCAGTTGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACgg                                                                                                                                                                                                       <  1:631148/298‑1 (MQ=255)                                                                                                     tATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGagaa                                                                                                                                                                                                   >  2:632025/1‑301 (MQ=255)                                                                                                                    gTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGa                                                                                                                                                                                    >  2:366951/1‑301 (MQ=255)                                                                                                                              gcgTCGGATGTTATCAATGACTTCTGTGTTAATAATGGCGGACGCGGTAAAAATACGTCCGCCGTGAGCGATGCGGTGGCCAAGTCAGGCCACACTGTCTGTTAAATTCCGTTTTTCCCGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAGTGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCAGGCATCGAGCACGGAAAACGTAATCGAAGGCGAACCACAGTta                                                                                                                                                                          <  1:535460/301‑1 (MQ=255)                                                                                                                                             aaTGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGAATCGAGCACGGAAAACTTAATCGAAGACGAACAACAGTTAAAAACCAAaaaaaca                                                                                                                                                           >  2:428593/1‑300 (MQ=255)                                                                                                                                              aTGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAACAAAACAAccc                                                                                                                                                          >  1:257458/1‑300 (MQ=255)                                                                                                                                                          tcgctcacggcggcagtatttttaccgagtccgccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAAttcattca                                                                                                                                              >  2:313774/31‑301 (MQ=255)                                                                                                                                                                atGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTCGCCAATTAAGGCCACACTGTCATTTAAATTCAGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAAGACCTGACATTAATCCTTCACAGTCGCTGGCATCGCACCAGGAAAACTTAATCGAAGACACACCACAGTTCATAACCAAAACAACCGGAAATTCATTCAACCCtt                                                                                                                                        >  1:547897/1‑301 (MQ=255)                                                                                                                                                                        cTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCAAGCACGGAAAACTTAAACGAAGACGAACCACAGTTAATAACCAAAACAACAGGAAATTCATTCAACCCTTAtccaatc                                                                                                                                >  2:153306/1‑296 (MQ=255)                                                                                                                                                                         tCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATcc                                                                                                                               <  2:237302/301‑1 (MQ=255)                                                                                                                                                                                       gccgccGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAAAAACCGGAAAATACATCATCTCTTTTCCCATCCTGACTTACGGAttt                                                                                                                 >  1:211240/1‑300 (MQ=255)                                                                                                                                                                                        ccgccgttggggagtcGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGGTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTaa                                                                                                                <  1:425805/286‑1 (MQ=255)                                                                                                                                                                                                aatgacagtgtggccttaattggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGCTAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTACTCATCCTGAGTTACAGAATAAAACAGttt                                                                                                        >  1:530655/22‑301 (MQ=255)                                                                                                                                                                                                 gCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTg                                                                                                       <  2:257726/301‑1 (MQ=255)                                                                                                                                                                                                       gtgtggccttaattggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTCATAACCAAACCAACAGGACATTCATTCATCCCTTTTCCCATCCTGAGTTACGGAATAAAACCGtctcaaaacc                                                                                                 >  1:299084/15‑292 (MQ=255)                                                                                                                                                                                                           ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGt                                                                                             >  2:394152/1‑301 (MQ=255)                                                                                                                                                                                                            gCCAATGAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCGTCGTAGCGGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATGTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAAGACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAATACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGtt                                                                                            <  1:515728/301‑1 (MQ=255)                                                                                                                                                                                                                                aaTTTAAATTCTGTTTTTCCTGTTCAAGTGCAATTGCCTTCAAGGCACCTGCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAGGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCaa                                                                        <  1:177868/300‑1 (MQ=255)                                                                                                                                                                                                                                        ttCCGTTTTTCCAGTTCTAATGTGATTGCCTTCGATGCACCTTCGTAGCTGTGGTGAGCTAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCTAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTa                                                                <  1:153155/301‑1 (MQ=255)                                                                                                                                                                                                                                           cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTaaca                                                             >  2:234195/1‑301 (MQ=255)                                                                                                                                                                                                                                                                   gCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGACGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGAATGGTCAGCAGACCAATCACAGTAACAAACACGTTATCCAAACGACCACgg                                     >  2:489777/1‑301 (MQ=255)                                                                                                                                                                                                                                                                       tCAATGCACCTTCTTAGCTGTGGTGAGCCAGCGGTGCTGTCTCTCCCCCATTTACGGATAAGAATGCATTTTCCTAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTAtt                                 <  2:207610/301‑1 (MQ=255)                                                                                                                                                                                                                                                                            gCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAAAAAACACGTTATCCAACAGACCACGGTATTTCGcc                            >  1:550774/1‑301 (MQ=255)                                                                                                                                                                                                                                                                               tgtgctcttccgatctgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCaga                         <  2:552111/286‑1 (MQ=255)                                                                                                                                                                                                                                                                               ccTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCACTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAACACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACAAACACGGTATTTCGCCaga                         >  1:403540/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                     tAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTACTACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAAACGACCACGGTATTTAGCCAAAGACggg                   >  1:50792/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              agAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAAAACGTTATCCAGACGACCAAGGTATTTCGACAGAGACGGCGCTTTACgg          >  2:219756/2‑301 (MQ=255)                                                                                                                                                                                                                                                                                               gAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGACaaaagaaccgaaaagcggt         >  1:551438/1‑283 (MQ=255)                                                                                                                                                                                                                                                                                               gAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACAACGGTATTTCGCCAGAGACGACGCTATACGGa         >  1:230213/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                aGCCAGCACCGCTGGCTCTCCCCCATTTACGTATAAGAATTCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGAt        <  1:219513/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATg       >  2:593410/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                    aGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCAAAACGACCACGGTATTTCGCCAGAGACGGCGATTAACGGATGGCa    >  1:211072/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                    aGCGGTGCTGGCTCTCCCCCACTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCa    <  2:404103/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      tggTGTTGGCTCTCCCTCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATa  <  2:558871/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                            GGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATA  >  NC_000913/3263206‑3263799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑