Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,263,501 G→C 9.4% P54A (CCG→GCG)  tdcD ← propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,263,5010GC 9.4% 161.5 / 8.0 55P54A (CCG→GCG) tdcDpropionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (27/22);  new base C (2/3);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATA  >  NC_000913/3263206‑3263799
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          
ggATCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGGGCCAGTGGAGAAACGTGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGGGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTg                                                                                                                                                                                                                                                                                                       <  2:3150/301‑1 (MQ=255)
       ttgTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGCTCAcc                                                                                                                                                                                                                                                                                                <  2:429198/299‑1 (MQ=255)
        cGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGAGAGCCAGCACCGCTGGCTCAcca                                                                                                                                                                                                                                                                                               <  2:392309/301‑2 (MQ=255)
         agacgtgtgctcttccgaTCTAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc                                                                                                                                                                                                                                                                                              <  2:355159/284‑1 (MQ=255)
                        gTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAACACTGCCGCCGTGAGCGATGCGATTGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATACAATTGCCTTCAATGCACCTTCGTACCTTGAGTGCGCCAGCGATTCTGGCTCTCCCCCATTTACGTATAAGa                                                                                                                                                                                                                                                                               >  1:558157/1‑301 (MQ=255)
                         tATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGaa                                                                                                                                                                                                                                                                              <  1:161842/301‑1 (MQ=255)
                              aaTACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTccccagatcggaaacgcagcgtggt                                                                                                                                                                                                                                                                         >  1:354675/1‑280 (MQ=255)
                                tACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCAGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCAACTTCGTAGCTGTAGTGAGCCAGCGGTGCTGGCTCTCACCCATTTACGGATAAGAATGCAttt                                                                                                                                                                                                                                                                       >  1:207367/1‑301 (MQ=255)
                                aaCCGCCACCTGAGTTTCGCCTGGAAATAATTGCTGCGCCGATTTAATACCACTTAAATTGGCGTAATTATGGAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACGCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAttt                                                                                                                                                                                                                                                                       <  1:140121/300‑1 (MQ=255)
                                      gtgtgctcttcctatctgAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAg                                                                                                                                                                                                                                                                 <  2:552340/284‑1 (MQ=255)
                                            ggTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATa                                                                                                                                                                                                                                                           <  2:84764/300‑1 (MQ=255)
                                                   cccGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTCAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGAACCTTCGTATCTGTTGTTAGCCAGCGGTGCTGGCTCTCCCCCATTTACGAATACGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                                                                                                                                                                                    >  1:344044/1‑301 (MQ=255)
                                                       gAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCACTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAgagatcggaagagcgtcc                                                                                                                                                                                                                                                >  1:551667/1‑284 (MQ=255)
                                                                 cgtttgctcttccgatctaCTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACAtt                                                                                                                                                                                                                                      <  2:584208/283‑1 (MQ=255)
                                                                  tgcGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATTCACCTTCGTCGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTa                                                                                                                                                                                                                                     >  2:426369/1‑301 (MQ=255)
                                                                    tgtgctcttccgatctcTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt                                                                                                                                                                                                                                   <  2:93119/285‑1 (MQ=255)
                                                                           tCAATTCCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcac                                                                                                                                                                                                                            <  1:55886/301‑1 (MQ=255)
                                                                           tCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAAGAAGGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcac                                                                                                                                                                                                                            <  1:217673/301‑1 (MQ=255)
                                                                                 acACTTAAATTTGCGTAATTATGCAGGGGGGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGc                                                                                                                                                                                                                      <  1:498597/300‑1 (MQ=255)
                                                                                   aCTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTagaacggaagagcgtcgt                                                                                                                                                                                                                    >  1:583484/1‑284 (MQ=255)
                                                                                    cTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATagatcggaaaagcgtc                                                                                                                                                                                                                   >  1:92995/1‑286 (MQ=255)
                                                                                              cGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCaa                                                                                                                                                                                                         >  2:516400/1‑300 (MQ=255)
                                                                                                atcttATGCAGGGGTGCCAGTTGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACgg                                                                                                                                                                                                       <  1:631148/298‑1 (MQ=255)
                                                                                                    tATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGagaa                                                                                                                                                                                                   >  2:632025/1‑301 (MQ=255)
                                                                                                                   gTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGa                                                                                                                                                                                    >  2:366951/1‑301 (MQ=255)
                                                                                                                             gcgTCGGATGTTATCAATGACTTCTGTGTTAATAATGGCGGACGCGGTAAAAATACGTCCGCCGTGAGCGATGCGGTGGCCAAGTCAGGCCACACTGTCTGTTAAATTCCGTTTTTCCCGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAGTGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCAGGCATCGAGCACGGAAAACGTAATCGAAGGCGAACCACAGTta                                                                                                                                                                          <  1:535460/301‑1 (MQ=255)
                                                                                                                                            aaTGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGAATCGAGCACGGAAAACTTAATCGAAGACGAACAACAGTTAAAAACCAAaaaaaca                                                                                                                                                           >  2:428593/1‑300 (MQ=255)
                                                                                                                                             aTGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAACAAAACAAccc                                                                                                                                                          >  1:257458/1‑300 (MQ=255)
                                                                                                                                                         tcgctcacggcggcagtatttttaccgagtccgccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAAttcattca                                                                                                                                              >  2:313774/31‑301 (MQ=255)
                                                                                                                                                               atGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTCGCCAATTAAGGCCACACTGTCATTTAAATTCAGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAAGACCTGACATTAATCCTTCACAGTCGCTGGCATCGCACCAGGAAAACTTAATCGAAGACACACCACAGTTCATAACCAAAACAACCGGAAATTCATTCAACCCtt                                                                                                                                        >  1:547897/1‑301 (MQ=255)
                                                                                                                                                                       cTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCAAGCACGGAAAACTTAAACGAAGACGAACCACAGTTAATAACCAAAACAACAGGAAATTCATTCAACCCTTAtccaatc                                                                                                                                >  2:153306/1‑296 (MQ=255)
                                                                                                                                                                        tCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATcc                                                                                                                               <  2:237302/301‑1 (MQ=255)
                                                                                                                                                                                      gccgccGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAAAAACCGGAAAATACATCATCTCTTTTCCCATCCTGACTTACGGAttt                                                                                                                 >  1:211240/1‑300 (MQ=255)
                                                                                                                                                                                       ccgccgttggggagtcGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGGTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTaa                                                                                                                <  1:425805/286‑1 (MQ=255)
                                                                                                                                                                                               aatgacagtgtggccttaattggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGCTAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTACTCATCCTGAGTTACAGAATAAAACAGttt                                                                                                        >  1:530655/22‑301 (MQ=255)
                                                                                                                                                                                                gCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTg                                                                                                       <  2:257726/301‑1 (MQ=255)
                                                                                                                                                                                                      gtgtggccttaattggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTCATAACCAAACCAACAGGACATTCATTCATCCCTTTTCCCATCCTGAGTTACGGAATAAAACCGtctcaaaacc                                                                                                 >  1:299084/15‑292 (MQ=255)
                                                                                                                                                                                                          ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGt                                                                                             >  2:394152/1‑301 (MQ=255)
                                                                                                                                                                                                           gCCAATGAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCGTCGTAGCGGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATGTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAAGACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAATACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGtt                                                                                            <  1:515728/301‑1 (MQ=255)
                                                                                                                                                                                                                               aaTTTAAATTCTGTTTTTCCTGTTCAAGTGCAATTGCCTTCAAGGCACCTGCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAGGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCaa                                                                        <  1:177868/300‑1 (MQ=255)
                                                                                                                                                                                                                                       ttCCGTTTTTCCAGTTCTAATGTGATTGCCTTCGATGCACCTTCGTAGCTGTGGTGAGCTAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCTAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTa                                                                <  1:153155/301‑1 (MQ=255)
                                                                                                                                                                                                                                          cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTaaca                                                             >  2:234195/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                  gCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGACGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGAATGGTCAGCAGACCAATCACAGTAACAAACACGTTATCCAAACGACCACgg                                     >  2:489777/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                      tCAATGCACCTTCTTAGCTGTGGTGAGCCAGCGGTGCTGTCTCTCCCCCATTTACGGATAAGAATGCATTTTCCTAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTAtt                                 <  2:207610/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAAAAAACACGTTATCCAACAGACCACGGTATTTCGcc                            >  1:550774/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                              tgtgctcttccgatctgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCaga                         <  2:552111/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ccTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCACTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAACACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACAAACACGGTATTTCGCCaga                         >  1:403540/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                    tAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTACTACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAAACGACCACGGTATTTAGCCAAAGACggg                   >  1:50792/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                             agAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAAAACGTTATCCAGACGACCAAGGTATTTCGACAGAGACGGCGCTTTACgg          >  2:219756/2‑301 (MQ=255)
                                                                                                                                                                                                                                                                                              gAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGACaaaagaaccgaaaagcggt         >  1:551438/1‑283 (MQ=255)
                                                                                                                                                                                                                                                                                              gAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACAACGGTATTTCGCCAGAGACGACGCTATACGGa         >  1:230213/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                               aGCCAGCACCGCTGGCTCTCCCCCATTTACGTATAAGAATTCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGAt        <  1:219513/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATg       >  2:593410/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                   aGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCAAAACGACCACGGTATTTCGCCAGAGACGGCGATTAACGGATGGCa    >  1:211072/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                   aGCGGTGCTGGCTCTCCCCCACTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCa    <  2:404103/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     tggTGTTGGCTCTCCCTCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATa  <  2:558871/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          
GGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATA  >  NC_000913/3263206‑3263799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: