Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,335,655 T→A 10.4% intergenic (‑65/+39) eptA ← / ← adiC lipid A phosphoethanolamine transferase/arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,335,6550TA10.4% 134.5 / 5.9 51intergenic (‑65/+39)eptA/adiClipid A phosphoethanolamine transferase/arginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base A (3/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

GCCAGTGGTCGATTAAGCCATAAGAAAGAGCTTAGTGTCAGGACAATATTAATCACGCTGAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGCACAGGTGTAAAGATATGGCACCAGT  >  NC_000913/4335355‑4335949
                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                      
gcCAGTGGTCGATTAAGCCATAAGAAAGAGCTTAGTGTCAGGACAATATTAATCACGCTGAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAACGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAAACTTAAGtt                                                                                                                                                                                                                                                                                                        >  2:135135/1‑301 (MQ=255)
      ggTCGATTAATCCATAAGAAATAGCTTAGTGTCAGTACAATATTAATCACGCTGAAAGCGACGACCGGCAGCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAg                                                                                                                                                                                                                                                                                                  <  1:396490/301‑1 (MQ=255)
           tttAAGCCATAAGAAAGAGCTTAGTGTCAGGACAATATTAATCACGCTGAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGAACTTAAGGTTgg                                                                                                                                                                                                                                                                                             <  2:618019/300‑1 (MQ=255)
             tttcagacgtgtgctcttccgatctCAGGACAATATTAATCACGCTGAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAACATAAAAGCCAACCTTAAGAACTTAAGGTTgg                                                                                                                                                                                                                                                                                             <  2:538669/277‑1 (MQ=255)
                   aTAAGAAAGAGCTTAGTGTCAGGAAAATATTAATCACGCTGAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATg                                                                                                                                                                                                                                                                                     <  1:211974/301‑1 (MQ=255)
                         aatagCTTGGTGTCAGGACAATATTAATCACGCTGAAAGCGACGACCGGCATCGACAATAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCt                                                                                                                                                                                                                                                                               <  1:370579/298‑1 (MQ=255)
                            agCTTAGTGTCAGGACAATATTAATCACGCTGAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATATTTGCTGGa                                                                                                                                                                                                                                                                            >  2:508584/1‑301 (MQ=255)
                                    agttcagacgtttgctcttccgatctaGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAgggg                                                                                                                                                                                                                                                                    <  2:480152/275‑1 (MQ=255)
                                      cAGGACAATATTAATCACGCTGAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAACATAAAAGCCAACCTTAAGAACTTAAGGTTggaaaacggaagagcgtcgggaaggag                                                                                                                                                                                                                                                                    >  1:537996/1‑276 (MQ=255)
                                                 tAATCACGCTGAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCCAAGCAAAATTAAGCCAAACTTAAGTTCTTAAGGTTGGCTTTAATGTTTACTGGATACAAGGGAAATAAACTTTgt                                                                                                                                                                                                                                                       >  1:206173/1‑300 (MQ=255)
                                                           gAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGc                                                                                                                                                                                                                                             <  2:519458/301‑1 (MQ=255)
                                                              aGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGaaatcggaagagcgtcgggtagggaa                                                                                                                                                                                                                                          >  1:479551/1‑277 (MQ=255)
                                                                  acgacCGGGATAGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAAAACCTGTTTAAAAAAGGAAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAg                                                                                                                                                                                                                                      <  1:248658/301‑1 (MQ=255)
                                                                           aTCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCAAAGGGATACggg                                                                                                                                                                                                                             <  2:510890/301‑1 (MQ=255)
                                                                                   gAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTAtt                                                                                                                                                                                                                     >  2:556651/1‑300 (MQ=255)
                                                                                               ggtATGCAGCTAATCCAGCGGCAGCGCCTGTAACACTTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTa                                                                                                                                                                                                         <  1:555937/299‑1 (MQ=255)
                                                                                                    gCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAACGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTCAGGGGAATTAATATTTGCTTATTGGTGAATATAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTct                                                                                                                                                                                                    >  1:26123/1‑300 (MQ=255)
                                                                                                     cAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAg                                                                                                                                                                                                   <  2:390552/301‑1 (MQ=255)
                                                                                                             ttcagacgtgtgctcttccgatctttttAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACa                                                                                                                                                                                           <  2:533066/276‑1 (MQ=255)
                                                                                                                        gcCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatg                                                                                                                                                                                <  2:26174/301‑1 (MQ=255)
                                                                                                                                     gTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCAAACaagaccggaaaagcgtcgtggaggg                                                                                                                                                                   >  1:532393/1‑277 (MQ=255)
                                                                                                                                        tAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTACGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACaa                                                                                                                                                                >  1:461129/1‑301 (MQ=255)
                                                                                                                                                    tatTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATaa                                                                                                                                                    >  1:28111/1‑301 (MQ=255)
                                                                                                                                                     atTCAGGCAGATAGAGATAAAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTAGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTAATGACCAACAGGGAGACAAATGACCACATAAc                                                                                                                                                   >  1:154182/1‑301 (MQ=255)
                                                                                                                                                            ttgATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTc                                                                                                                                            <  2:38393/299‑1 (MQ=255)
                                                                                                                                                              tccgatcttataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAGGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGc                                                                                                                                          <  2:7511/293‑1 (MQ=255)
                                                                                                                                                              gATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTcag                                                                                                                                          >  1:38341/1‑299 (MQ=255)
                                                                                                                                                                      ttctaaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGAcc                                                                                                                                  <  2:28784/297‑1 (MQ=255)
                                                                                                                                                                      tataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAGGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATAAGGGTGACAAATGACCACAAAACCCCTTTCGcagatcgga                                                                                                                                  >  1:7511/1‑293 (MQ=255)
                                                                                                                                                                          aaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACAAAACCTCTTTCGCTCCGGAccagaa                                                                                                                              >  1:28733/1‑297 (MQ=255)
                                                                                                                                                                                        aTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGc                                                                                                                <  2:461693/301‑1 (MQ=255)
                                                                                                                                                                                             cAGGCGAGTAAATTCAAATAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTag                                                                                                           <  2:154333/301‑1 (MQ=255)
                                                                                                                                                                                                        aTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCaaa                                                                                                >  2:316642/1‑300 (MQ=255)
                                                                                                                                                                                                         ttCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGAAGAAGAGGAAGGCAATg                                                                                               >  2:149820/1‑301 (MQ=255)
                                                                                                                                                                                                          tCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCACTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGAAGAGGAAGGCAAAgg                                                                                              >  1:39847/1‑301 (MQ=255)
                                                                                                                                                                                                             aaGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATCCTCGCAAAGCAAAATTAAGCCAACCTTAAGTCCTTAAGGTTGGCTTTAACGTTTGCTGGATTAAGGGGAATTCATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCACATACATGGCGGTGATGACCATGAGGGTGACAAATGACCACATAACCTCATTCGCTCCGGACCCCAACACGGCCCACAAGCAGTAGAGGAAGGCAATGgaag                                                                                           >  1:18250/1‑301 (MQ=255)
                                                                                                                                                                                                                               ggCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACAACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAAATGCAAGATATGACg                                                                         >  2:285449/1‑301 (MQ=255)
                                                                                                                                                                                                                                   cgtCAACAGCTCGGTTTTTCCATCGAACAGAGTGCGAATATGATCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTTGCTTTTATGTGTGCTGGATTAAGGTGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGAGAATCAGGGTGACAAATGACCACATAACCTCTTACGCTCCGGACCACACCACGGCCCGGATGCAGTAGAGGAAGGCAAGGGGAGTAACTGCCAGATATggcgggcg                                                                     <  1:95258/299‑1 (MQ=255)
                                                                                                                                                                                                                                        aCATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCtt                                                                >  2:611220/1‑301 (MQ=255)
                                                                                                                                                                                                                                                    ttCCATCGAACAAAGTGCGCATATGCTCTCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGAc                                                    <  2:141360/301‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTCTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACAGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGAc                                                    >  2:349357/1‑301 (MQ=255)
                                                                                                                                                                                                                                                     tCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGAcc                                                   >  2:532838/1‑301 (MQ=255)
                                                                                                                                                                                                                                                       cATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTCGAGGAAGGCAATGGTAGAAACTGCCAGAAATGCCGGGCGTGATTTACCAAAGTAACCgg                                                 >  1:93486/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                   gctcttccgattttgCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAAGCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGCACAg                       <  1:533601/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         aaTTAAGTGTACCTTAATTTCTTAAGGTTGGCTTTTATGGTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTGTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGCACAGGTGTAAAg               <  2:18301/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         aaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCACGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGCACAGGTGTaaaa               >  2:161907/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                aGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGCACAgagatcggaagaga          >  2:534274/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                                 gCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGACAAGGAGTAAAGAAATgg         >  1:630166/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                  ccAACCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGCACAGGAGTAAAGATATgga        >  2:93840/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                   cAACCTTAAGAACTTAAGGTTGGCTTAATTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCCGGGTGACAACTGACCACCTAACCTCTTTCGCTCCGGACCCCACCCCGGCCCAGATGCCGTAGAGGAAGGCAATGGTAGTAACTGCCAGACATACCGGGCGTGCTTTACAAAAGTAACCACGTCCGAGCAACAATAACGCAGCACAACTGTAAAAAAATgacccc     >  1:512077/1‑296 (MQ=255)
                                                                                                                                                                                                                                                                                                       tttAAGTTCTTAAGGTTGGTTTTTATGTTTGCTGGATTTAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTGTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTGTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGCACAGGTGTAAAGATATGGCACCAg   <  1:532165/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                        ttAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGCACAGGTGTAAAGATATGGCACCAgt  <  2:93610/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                      
GCCAGTGGTCGATTAAGCCATAAGAAAGAGCTTAGTGTCAGGACAATATTAATCACGCTGAAAGCGACGACCGGCATCGACAAGAAAACCAGTACGTTATGCAGCGAATCCAGCGGCAGCGCCTGCAACACCTGTTTAAAAAAGGCAATATTCAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAACCTCTTTCGCTCCGGACCCCACCACGGCCCAGATGCAGTAGAGGAAGGCAATGGTAGTAACTGCCAGATATGCCGGGCGTGCTTTACCAAAGTGACCGTGTCCGAGCAGCAGTAACGCCGCACAGGTGTAAAGATATGGCACCAGT  >  NC_000913/4335355‑4335949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: