Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,367,702	A→C	5.7%	I209S (ATC→AGC)	aspA ←	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,367,702	0	A	C	5.7%	224.6 / 3.2	73	I209S (ATC→AGC)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (30/38);  new base C (3/2);  total (33/40)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

GGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTT  >  NC_000913/4367403‑4367997                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        ggCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGAAt                                                                                                                                                                                                                                                                                                        >  2:258711/1‑301 (MQ=255)         cGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAg                                                                                                                                                                                                                                                                                                <  2:291558/301‑1 (MQ=255)          ttGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAga                                                                                                                                                                                                                                                                                               <  2:225478/300‑2 (MQ=255)           tGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAgcg                                                                                                                                                                                                                                                                                              >  2:432432/1‑301 (MQ=255)                           aaGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTCACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATCCTGCAAGCGCGGAATTCCTGACCgc                                                                                                                                                                                                                                                                              >  1:267008/1‑300 (MQ=255)                              gtgaGGTGGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTAAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGGTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAggg                                                                                                                                                                                                                                                                           <  1:426541/298‑1 (MQ=255)                                  ggTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTATTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTcct                                                                                                                                                                                                                                                                       >  1:361580/1‑299 (MQ=255)                                    gtgTTACAGATGTTGGGCATCGTGCCAGACAGGAGTTTTAGATCGCCGGGAACAATCACATTAGGGCAGCAGTAATAGGTAGCTTCGTGTATGTCGTCGTCCGGTACGCATGGGAAGCTGGTAACGTAAGCTAGTTTTTTCACTGCCAGCGGAGAGTACTCGGTCGGCGTGTACAGACCAGGACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTAGTTCAGCAGGATGCGGAAAGCGCGGAATTCCTGAccctcggtcagga                                                                                                                                                                                                                                                                     <  1:450561/299‑12 (MQ=255)                                                   gACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCCGTTCAGCGGTACGGTGGATGTTTTTAACATCTTCTTTCAGCCGGCAGCTGAAACCGCGGAATTCCTGCCCGAAGGTCATAGGGAATGCGTCATGcc                                                                                                                                                                                                                                                      >  1:606424/1‑300 (MQ=255)                                                       tCTTCACAGCCAGGCGTTTCAGCGCGACGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCGTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTg                                                                                                                                                                                                                                                  <  2:267276/301‑1 (MQ=255)                                                        tttCACAGCCAGTCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTTAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTgg                                                                                                                                                                                                                                                 <  2:209300/300‑1 (MQ=255)                                                        cTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTgg                                                                                                                                                                                                                                                 >  1:306640/1‑301 (MQ=255)                                                             caGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACg                                                                                                                                                                                                                                            >  2:246201/1‑301 (MQ=255)                                                                  aGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCCCTTCTTCTTTCAGCCGGATGCTGAAACAGCGGAATTCCTAACCCAGGGTCATCGGTACTCCTTCCTCCAGCTGGTTACCAcccc                                                                                                                                                                                                                                       >  1:432750/1‑300 (MQ=255)                                                                   cacccgacgcttttccgctctccATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCAt                                                                                                                                                                                                                                      <  1:642149/280‑1 (MQ=255)                                                                    gCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCAtt                                                                                                                                                                                                                                     <  2:463907/301‑1 (MQ=255)                                                                       tttCGGGGCGCCGTGAACCATAACGTAAGCGCCGCAGTCAGAGGTCGCTTCGTTCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCGTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTc                                                                                                                                                                                                                                  <  1:431868/301‑1 (MQ=255)                                                                       tttCAGAGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTc                                                                                                                                                                                                                                  <  1:239451/301‑1 (MQ=255)                                                                          cAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCGTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAgg                                                                                                                                                                                                                               <  1:95166/301‑1 (MQ=255)                                                                           cgctcgcCGTTAACCATATCATAAGCGCCGCTGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGa                                                                                                                                                                                                                              <  2:347585/297‑1 (MQ=255)                                                                               cgcCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTc                                                                                                                                                                                                                          >  1:209001/1‑301 (MQ=255)                                                                                      agccATGACATAAGCGCCGCAGTCAGAGGTCGTTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTGTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAAt                                                                                                                                                                                                                   <  1:494148/299‑1 (MQ=255)                                                                                        ccATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCAtagatcggaagagcacacgtct                                                                                                                                                                                                                 >  2:643026/1‑280 (MQ=255)                                                                                         cataacataaGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTACAACTGGGTACGACCCATTTTCAGGATGTCCTGGAAATCg                                                                                                                                                                                                                >  1:88751/1‑301 (MQ=255)                                                                                           gacgcttttccgatctgTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGAc                                                                                                                                                                                                              <  1:44717/285‑1 (MQ=255)                                                                                             acataaGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGAACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTCCAGGATGTCCTGGAATTCGACAg                                                                                                                                                                                                            >  1:182099/1‑301 (MQ=255)                                                                                             aacgtagggccgcTGTGAGTGGTCGCTTTGATTAGGTCTTCTGCCGGTACGCATGGTAAGCCAGTAACTTCAGCCAGTTTTGTCACTGCCAGCGGAGAGTACTCTGTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACGTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAg                                                                                                                                                                                                            <  1:641762/293‑1 (MQ=255)                                                                                                gaagcgacctctgactgcggcGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTACTGGAATTCGACAGAtt                                                                                                                                                                                                         >  2:504788/21‑301 (MQ=255)                                                                                                    cgccgcAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTATGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACg                                                                                                                                                                                                     <  2:302956/301‑1 (MQ=255)                                                                                                    cgccgcAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACg                                                                                                                                                                                                     <  2:227864/301‑1 (MQ=255)                                                                                                          aGTAAGAGGTGGTTTCGAGTAGGTCTGCTGGCGTGACGCATGGTAGGCCAGTAACTTCAGCCAGTTGTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGAGCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTGCACTTCTTCGTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCaaa                                                                                                                                                                                               <  1:325039/301‑1 (MQ=255)                                                                                                           gTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAgatcggaagagcaca                                                                                                                                                                                              >  2:44770/1‑287 (MQ=255)                                                                                                           gTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAg                                                                                                                                                                                              >  1:265443/1‑301 (MQ=255)                                                                                                                    cGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTAACGc                                                                                                                                                                                     >  2:241660/1‑301 (MQ=255)                                                                                                                      cTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCaa                                                                                                                                                                                   >  2:463120/1‑300 (MQ=255)                                                                                                                           ttCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGt                                                                                                                                                                              <  2:265711/300‑1 (MQ=255)                                                                                                                               ggTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAAt                                                                                                                                                                          <  2:362064/301‑1 (MQ=255)                                                                                                                                 tCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGCATTCGACAGCTTTACGTTCAACGCCTTCACACAGTTGTTTAATcc                                                                                                                                                                        >  1:168219/1‑300 (MQ=255)                                                                                                                                      aGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCCGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGCATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGCCCCATTTTCAGTATGTCCTGGAATTCGACAGCTTTACGTTCAAAACCTTccagcaactgcataatcacacct                                                                                                                                                                   >  1:542461/1‑279 (MQ=255)                                                                                                                                        ccGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTAc                                                                                                                                                                 <  2:433314/301‑1 (MQ=255)                                                                                                                                           gttcGCATGGGAAGCCAGTAACTTCAGCCATTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAg                                                                                                                                                              <  1:108302/298‑1 (MQ=255)                                                                                                                                                 aTGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTaaa                                                                                                                                                        >  2:36783/1‑300 (MQ=255)                                                                                                                                                  gtggAAGCCTGTAACTTTATCCAGTTTTTTCAGTGCCAGCGGATAGTACTCGTTCGGCGTGTTCAGACCAGGACCGAGTTCTGGTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATc                                                                                                                                                       <  1:444496/299‑1 (MQ=255)                                                                                                                                                    ggATGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAg                                                                                                                                                     <  1:245934/301‑1 (MQ=255)                                                                                                                                                      aaGCCAGTAACTTCGTCTAGTTTTGTCACTGCCAGCGGGGAGTACTCTTTCGGGGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCGAGCAGCAGTTCAGCGGTACGTTGGATGTTGTTCACTTCTTCGTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAggg                                                                                                                                                   <  1:490148/301‑1 (MQ=255)                                                                                                                                                                cTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGAAAGAGAAAAc                                                                                                                                         >  1:208340/1‑301 (MQ=255)                                                                                                                                                                   cAGCCAGTTGTTTCTCTGCCAGCTGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGc                                                                                                                                      <  2:607186/301‑1 (MQ=255)                                                                                                                                                                             tttCCCTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGaaa                                                                                                                            <  1:36732/301‑1 (MQ=255)                                                                                                                                                                                cACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTAAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTAACGCAGTTGGTTAATCGCATCAACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCg                                                                                                                         >  1:284009/1‑301 (MQ=255)                                                                                                                                                                                     ccAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCgg                                                                                                                    >  2:215603/1‑301 (MQ=255)                                                                                                                                                                                                      tttgtggtgtTCAGGCCAGTACTGGTTGCTGTTGAACCAATGTTAACTTCCGGCAGCAGTTCAGGTGTACTTTGGATGTTTTTCTCTTCTTCTGTCGGCAGGATGCTGAAAGCGCGGAATTCCTGACCTACGGTCATCGGTAATGCGTCGTGCCGCTGGGTTCGACCCATGTTCAGGATGGTCTGGAAGTCGACAGCTTTACGTTCAAAGCCGTCGCGCAGTTGGTTAATAGCGTCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGGTAGTgg                                                                                                   <  1:460607/295‑1 (MQ=255)                                                                                                                                                                                                      ttCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTgg                                                                                                   <  2:307044/301‑1 (MQ=255)                                                                                                                                                                                                      ttCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTgg                                                                                                   <  2:209244/301‑1 (MQ=255)                                                                                                                                                                                                      ttCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACGGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTgg                                                                                                   <  1:559834/301‑1 (MQ=255)                                                                                                                                                                                                          cccctacagatatctcttccgatcttGTTTCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGTCTTCGTTAGTGGACtt                                                                                               <  1:113851/276‑2 (MQ=255)                                                                                                                                                                                                                     ccggTACCGTTTTCTGTTGCACCAATGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTaa                                                                                    <  1:504116/298‑1 (MQ=255)                                                                                                                                                                                                                         tACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATg                                                                                <  2:182296/301‑1 (MQ=255)                                                                                                                                                                                                                                  cTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCgg                                                                       >  1:496652/1‑301 (MQ=255)                                                                                                                                                                                                                                         aCCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAgg                                                                >  2:170055/1‑301 (MQ=255)                                                                                                                                                                                                                                                      tCCAGCAGCAGTTCAGCGTTACGTTTGATGTTTTTCACTTCTTCTTTCAGCAGTATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCAc                                                   <  2:284312/301‑1 (MQ=255)                                                                                                                                                                                                                                                               agttcagacttgtgctcttccgagttCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCGTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGt                                          <  2:350383/277‑1 (MQ=255)                                                                                                                                                                                                                                                                ttttaGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTg                                         <  2:88875/297‑1 (MQ=255)                                                                                                                                                                                                                                                                       ggTACTTTGGTTGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATc                                  <  1:169858/301‑1 (MQ=255)                                                                                                                                                                                                                                                                             ttGGTTTTTTTTCATTTCTTCTTGTAGCAGGATGGTGAAAGCGTGGAAGTGCTGACCGGGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTGCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTGcc                            <  1:69514/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ttGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTAACAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGAc                        <  2:497299/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         cACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCACAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTACTCAGGGACGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGAAATTTGTTAACATGGTCGTACGTGTTCAGGTACTGATATTCACCTTTTTggaagatcggaagagcgccgtgtagggaa                >  1:349920/1‑274 (MQ=255)                                                                                                                                                                                                                                                                                             tcttcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCa            >  2:630773/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                              gcgcttTCAGCATCCTGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGGCGTTAGTGGACTGACCTTTGTTAACATGGTCGTTCGGGTTCCGGTACCGCAATTCACCTTTTTGGTGACCCACACGTTCCaatccgcaatttgcccc           >  1:8231/4‑285 (MQ=255)                                                                                                                                                                                                                                                                                                 cttTCAGCATCCTGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGACTTCGACAGCTTTACGTTCAAAGCCTTCCCGCAGTTGGTCAATCGCATCTACCACCTTAATCAGGCACGAGTAAACTGCGCTACGTAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACCTGGTCTTTCGGCTTCAGGTACTGATATTCACCTTTTTGCTGACCCATCAGTTCAAACCCGATATAGTCCAGCAc        >  1:445743/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                    tCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCAATTc     >  2:538095/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                      agcagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTTACCCATCAGTTCCAGACCGATATGGGCCAGCACTTCGt   >  1:521712/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                       gcagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGTATTCGACAGCTTTACGTTCAAAGCCTTCACGCCGTTGGTTAATCGCATCTACCATCTTAATCAGGGCAGCGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGCCGTTAGTGGACTGACATTTGTTAACATGGTCTTTCGGGTTCCGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACTAATATTGCCCAGCACTTCGtt  >  1:115310/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                       gcagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTTTTTAATCGCATCTACCCTCTGAATCAGGGACGCTTAAACTGCTCTCCTCAAACCGGTCCTTTAGGCGTCGTTAGTGTCCTGACATTTGTTACCATGGTCGTTCGGGTTCCGGTACTGCTATTCACCTTTTTGGTGACCCCTCCATTCCAAACCCATATTGCCCAGcccctcctt  >  1:363418/1‑293 (MQ=255)                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        GGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGGGAAGAGTAAACTGCGATACGGAAACCGGTCGGGTAGGCGTCGTTAGTGGACTGACATTTGTTAACATGGTCGTTCGGGTTCAGGTACTGATATTCACCTTTTTGGTGACCCATCAGTTCCAGACCGATATTGGCCAGCACTTCGTT  >  NC_000913/4367403‑4367997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑