Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,464,327 A→T 8.0% I128N (ATC→AAC)  treC ← trehalose‑6‑P hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,464,3270AT 8.0% 206.4 / 2.9 73I128N (ATC→AAC) treCtrehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand):  ref base A (37/27);  new base T (2/5);  total (41/32)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCG  >  NC_000913/4464041‑4464620
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                     
ttAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGTTTTATATCTGGCgc                                                                                                                                                                                                                                                                                         <  2:545039/300‑2 (MQ=255)
  aGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTa                                                                                                                                                                                                                                                                                       >  2:541354/1‑301 (MQ=255)
   gAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAAATGGCGGTaa                                                                                                                                                                                                                                                                                      >  2:227914/1‑301 (MQ=255)
     aGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑AGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAACAAGATGAAACTAGTACTGTTCGATTTCCGCATGCCAGCGCCAAGCACTACCGCACAATTTTGAACGCAAATTGTTCGGTGGCGTTTCAGTTTCACCACCACACCAGATATAAAACTGGcaatacgg                                                                                                                                                                                                                                                                                    >  1:352655/1‑294 (MQ=255)
             ccGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGctttctt                                                                                                                                                                                                                                                                            >  2:213863/1‑301 (MQ=255)
             ccGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGctttctt                                                                                                                                                                                                                                                                            <  2:529588/301‑1 (MQ=255)
              cGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAACTAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATAGTGCACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATAAAAAAATAGACGAAAGGGcttacttt                                                                                                                                                                                                                                                                           >  1:579325/1‑301 (MQ=255)
                  gCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTAACGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATTGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCCGTTGATCGGTGGCGTTTCTGGTTCTCCATCGCGCAAAGAATAAAACTGGAGGTAAGGGcctactatgatc                                                                                                                                                                                                                                                                       >  1:132934/1‑290 (MQ=255)
                   ccGTCTAGGTCGTCATGGAAACGCGTGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCa                                                                                                                                                                                                                                                                      <  2:353118/301‑1 (MQ=255)
                       ccAGGTCTTCAGGGAAACGCGTGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAgcgc                                                                                                                                                                                                                                                                  <  2:258315/301‑1 (MQ=255)
                        cAGGTCTTCAGGGAAACACGGCTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GCCCGGCCCAGAACTCACACACTTTTTTCCGCTCTCCGCGTACCGC‑TCGCTTCTCCCAGTTGAGCTACGCCTGTCCTGGTGCCAAGAGATGCACATAGTACTCTCCGCCTTCCGCATGCCAGCGCCACGCCCTCCCGCAAAACTTTGCACCCCTGTCGTTCGATGGCGTTTCTGGTCAACCATCACACCATATATACAACTGGCGCTAAGGCCCTTCATTGCTCAGCGcc                                                                                                                                                                                                                                                                 >  1:113757/1‑301 (MQ=255)
                               tCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGaa                                                                                                                                                                                                                                                          <  2:393132/301‑1 (MQ=255)
                                            ggTCTTTGGAGATCAGATTCACCACCTCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTg                                                                                                                                                                                                                                             <  1:227671/301‑1 (MQ=255)
                                             gTCTTTGGTGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTgg                                                                                                                                                                                                                                            >  1:494007/1‑301 (MQ=255)
                                             gTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAACGCCTCGCGAAACCAGGCAGGTTgg                                                                                                                                                                                                                                            >  1:526326/1‑301 (MQ=255)
                                               ttttcccgtcttggtttaacacaACCAAGCTCAAACCGTTGACCCCACGGACCGGCCCAG‑ACTTAAAGACTTTTTTTAGGTCTGGGCGTTCCGCTTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTggg                                                                                                                                                                                                                                           <  2:515502/282‑1 (MQ=255)
                                                   ggAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCCAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGCTATAAAACTGGCGGGAAGGGCTTTCTTTGTTCAACGCCTCGCCAAACCAGGCATGTTGGGTcgag                                                                                                                                                                                                                                      >  1:636229/1‑301 (MQ=255)
                                                            aTTCCCCCCATCCAGGCGCAACCCGTCGACCCCAC‑GTTCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTa                                                                                                                                                                                                                             <  1:306513/301‑1 (MQ=255)
                                                               caccacATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCTCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTaaaa                                                                                                                                                                                                                          >  2:20145/1‑300 (MQ=255)
                                                                   acaTCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCa                                                                                                                                                                                                                      <  2:189399/301‑1 (MQ=255)
                                                                    caTCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGATAAACACCaa                                                                                                                                                                                                                     >  2:572524/1‑300 (MQ=255)
                                                                         aGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCga                                                                                                                                                                                                                >  2:264671/1‑301 (MQ=255)
                                                                             gcAACCCGTCGTCCCCAC‑GATCGGCCCAGAACTCACAGGCGTTTTTCAGCTCTGCGCGTACCGC‑TGGATCTTCCCAGTTGAGATCCGCCTGTTCTGGGGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAAt                                                                                                                                                                                                            <  1:205978/301‑1 (MQ=255)
                                                                                              cctaTCTGCCTAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTCTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCcgcgc                                                                                                                                                                                            <  2:133059/298‑1 (MQ=255)
                                                                                                          gAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGAGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCAAAACGAGAATAAAACAAATCCCGCACGATTTTGCCTGc                                                                                                                                                                                >  1:115430/1‑301 (MQ=255)
                                                                                                               cacaGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAACTTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCCGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATCCGAATCCCCCGCACATTTGCCTGCGCCAc                                                                                                                                                                           >  1:456627/1‑301 (MQ=255)
                                                                                                               cacaGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGCAACGAATCCCCCGCGATTTTGCCTGCATCAc                                                                                                                                                                           >  1:373518/1‑301 (MQ=255)
                                                                                                                acaGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCAcc                                                                                                                                                                          <  2:526998/301‑1 (MQ=255)
                                                                                                                     cTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTc                                                                                                                                                                     >  2:437805/1‑301 (MQ=255)
                                                                                                                      ttttttCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCg                                                                                                                                                                    <  2:115554/301‑1 (MQ=255)
                                                                                                                         tttCAGCTCTGGGGGTACCGC‑TGGATTCTCCCGGTTGAGATCGTTCTTTTCTTGGGAAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCGAATGTAGAACGCCAGTCGTTCGGGGGTGTGTCTGGTGCGTCAGCGGGGCAGAAATAACACTGGCGGTAAGGGATTTCTTTGTTCAGCACCAGGAGAAACCAGGCATGTTGGGTAGAGGTAAGGTTAAACACCATATCGAGAAGGATACGAATCCCGCGCGATTGTGCCTGCGTCACCAGTTCGtga                                                                                                                                                                 <  1:429657/301‑3 (MQ=255)
                                                                                                                            cAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAACCCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTCCCTGCGTCCCCAGTTCGTCaaaa                                                                                                                                                              >  1:173558/1‑301 (MQ=255)
                                                                                                                                        tACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTTTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACGAGTTCGTCAAAATCGTCCAGCGTg                                                                                                                                                  <  1:264403/301‑1 (MQ=255)
                                                                                                                                                      tcCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGAt                                                                                                                                     <  2:494653/301‑1 (MQ=255)
                                                                                                                                                       cccAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATc                                                                                                                                    >  1:283010/1‑301 (MQ=255)
                                                                                                                                                               agaTCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCg                                                                                                                            <  2:457191/301‑1 (MQ=255)
                                                                                                                                                                 aTCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGta                                                                                                                          >  2:570729/1‑301 (MQ=255)
                                                                                                                                                                              ggTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCCCCAGTTCGTCCCAATCGTCCAGCGTGCCGTAGGAGGGATCACACGCCGTATAGTTCGCtcctc                                                                                                             >  1:84733/1‑297 (MQ=255)
                                                                                                                                                                              ggTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGACACAATCGCCgacaaattcaccacgt                                                                                                             >  1:66440/1‑286 (MQ=255)
                                                                                                                                                                                ggaaaaaggattgtaaTTGTACTGTTCGCTTTCCGTATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTAcgtcg                                                                                                           <  1:540681/287‑1 (MQ=255)
                                                                                                                                                                                                     cTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGga                                                                                      >  2:72406/1‑301 (MQ=255)
                                                                                                                                                                                                          cGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCATTATCGACCTGGGgagagaa                                                                                 >  2:312223/1‑300 (MQ=255)
                                                                                                                                                                                                             tttCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATa                                                                              >  2:213930/1‑301 (MQ=255)
                                                                                                                                                                                                                 cGCATGCCTGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCTCGATTTTGCCTGCGTCACCCGTTCGTCAAAATAGTCCCGCGTGCCGTAGGTGGGAACAATCGCCGTATCGTTCGCTACCGCCACACCGTTCTCGCCCGTGGGAGAGCCATaacaa                                                                          >  1:1130/1‑298 (MQ=255)
                                                                                                                                                                                                                      gCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGAAATAAAAGGGGGt                                                                     >  2:208057/1‑301 (MQ=255)
                                                                                                                                                                                                                      gCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATAACGAGAATGATACGAATCCCGCGCGAATTTGCCTGCGTCACCAGTTCGTCAAAATCGTACAGCGTGCCGTAGGAGGGATCAATCGACGTATAGTTAGCTACGTAGTAACCGTAATAGAACTGGGGAGAACCAAAACAGAGGGt                                                                     >  1:125302/1‑301 (MQ=255)
                                                                                                                                                                                                                       ccAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGtt                                                                    >  2:161723/1‑301 (MQ=255)
                                                                                                                                                                                                                        tttcagacgtttgctcttccgttctttGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGA‑TTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAg                                                                  <  2:183978/277‑1 (MQ=255)
                                                                                                                                                                                                                                   aCTACCTCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCa                                                        <  2:84857/301‑1 (MQ=255)
                                                                                                                                                                                                                                    ttACCGCTAAATTTTGAACGCTAGTTGTTCGGTGTCGTTTCTGGTTCTCCATCGCGCCAGATATAGTACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCAt                                                       <  2:125426/300‑1 (MQ=255)
                                                                                                                                                                                                                                                tttGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTCAAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAAAGACATAAAAGGGGGTTAGCCACAAGACAACAACGCACAgat                                           >  1:134409/1‑299 (MQ=255)
                                                                                                                                                                                                                                                 ttGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGA‑TTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAgagaacggaagagcgacgtgtagggg                                         >  1:183737/1‑276 (MQ=255)
                                                                                                                                                                                                                                                  tGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTa                                         <  2:66530/301‑1 (MQ=255)
                                                                                                                                                                                                                                                    attgCCGGTGGTTCTGTGGCGTTTCTGGTTTTCCATCGTGCCAGATATAAAACTTGCGGTAAGGGCCTTCTTGGTTCTGGGCCTCGCGAAACCATGCATGTTGGGGAGAGGTATGGTTAAAGTCCATATCGAGAATGTTGCGAAGCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTAGAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGATATAAAAGGGGGTTAGGCAGATGGCATCAACGCCCAGTGTaag                                       <  1:20094/298‑3 (MQ=255)
                                                                                                                                                                                                                                                    aaCGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCACAGTTTATg                                       >  2:256894/1‑301 (MQ=255)
                                                                                                                                                                                                                                                     acgcatgttgttCGGTGGTTTTTTTTGTTTTCCATCTCGCCAGTTATAAAACTGGCGGTAAGGGCTTTCGTTGTTCGTCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGc                                      <  1:256626/295‑1 (MQ=255)
                                                                                                                                                                                                                                                      cGCCAGTTGTTCTGTGGCGTTTCTGTTTCTCCTTCGCGCCAGTTATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCATTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCa                                     <  1:311819/301‑1 (MQ=255)
                                                                                                                                                                                                                                                        ccaccgaacaactGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCNTCACCAGNNNNNNAAAATCGTCCAGNGTGCCGTAGGTGGGATCANACGCCGTATAGTTNNCTACGTCGTAACCGTAATCAACCTGGGGAGCAACATAAAACGGAGGACACCAGAAGGCAACAACCCACAgacgatgcaaa                                   >  1:268444/13‑291 (MQ=255)
                                                                                                                                                                                                                                                               ttCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAAAttgaccc                            >  1:108222/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                     ggCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAATCCAGGAGtt                      >  2:358743/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                      gCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTg                     <  2:173755/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ggTTTCTGGTTTGTCATCGCGCCAGATATAAAACTGGCGTTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTgg                    <  2:402281/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                        ttttCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGa                   <  2:637106/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                            tttgTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAAc               <  2:134534/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATaaa               >  2:507646/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                             ccttcgggtttaccgcCAGTTTTATATCTGGCGGTAAGGGCTTTCTGTGTTCAGCGCCTCGCGAAACCAGGCATTTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCTGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACg              <  1:399064/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                               gTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGAATAACGcc            >  2:604098/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                ttCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCa           <  2:580049/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 tttcCATCGCGCCAGTTATAAAGCTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCAc          <  1:161572/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    ccATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTa       >  1:224343/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                     cttaccgcCAGTTTTATATCTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCAAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTaa      >  2:270732/6‑301 (MQ=255)
                                                                                                                                                                                                                                                                                        accgcCAGTTTTATATCTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCTGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACgagatcggaaga   >  2:399578/3‑290 (MQ=255)
                                                                                                                                                                                                                                                                                         gcgcCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCAACCTGGGGAGAGACCTAAACAGGGGTTAGCCAAATGCCATCAACGCACAGTTAATGCAGATCGTCCAGGTGCAGGCTAACGcaccgcaacccc  >  1:373942/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                     
GTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCG  >  NC_000913/4464041‑4464620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑