Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,470,958 G→T 8.8% intergenic (‑45/+28) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,470,9580GT 8.8% 176.8 / 5.7 60intergenic (‑45/+28)ridA/pyrIenamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base G (30/24);  new base T (3/3);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

AAGTCGTTCAGATCTTTTACAAACACGGTAGTTTTAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGG  >  NC_000913/4470663‑4471257
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                           
aaGTCGTTCAGATCTTTTACAAACACGGTAGTTTTAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGcc                                                                                                                                                                                                                                                                                                        <  2:84658/301‑1 (MQ=255)
      ttccctacacgtcgctcttccgatcttttAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACtt                                                                                                                                                                                                                                                                                                  <  1:453100/276‑1 (MQ=255)
         aGATCTTTTACAAACACGGTAGTTTTAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGAATTtta                                                                                                                                                                                                                                                                                               >  2:424358/1‑301 (MQ=255)
                              gTTTTAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTgg                                                                                                                                                                                                                                                                          >  2:383782/1‑301 (MQ=255)
                                tttAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACttagatcggaagagcacacgtctgaacc                                                                                                                                                                                                                                                                        >  2:453664/1‑275 (MQ=255)
                                     gtaTGTCGCCCACTGTCAGTCCTGCGGTTTCGACGTTGGCTTTTACGTTGTCCAGCGATTGACGTGCCTGTGCAGCGACGTTTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGTACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcac                                                                                                                                                                                                                                                                   <  1:268261/299‑1 (MQ=255)
                                          tCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCacaacaa                                                                                                                                                                                                                                                              >  2:560476/1‑300 (MQ=255)
                                           cGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACAtt                                                                                                                                                                                                                                                             >  2:119304/1‑301 (MQ=255)
                                            gCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTa                                                                                                                                                                                                                                                            >  2:628013/1‑301 (MQ=255)
                                               cACTTTTAGTCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATgg                                                                                                                                                                                                                                                         <  2:296007/301‑1 (MQ=255)
                                                            gCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAACCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCAACACATTATGGAAAAAATCttttt                                                                                                                                                                                                                                            >  2:378243/1‑301 (MQ=255)
                                                               tcTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                                                                                                                                                                                                                         <  2:190636/300‑1 (MQ=255)
                                                                      cgaTCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACCCTTTTTCACAtttttt                                                                                                                                                                                                                                  >  1:5659/1‑301 (MQ=255)
                                                                       taTCGCTTTTACGTTATCCAGCTACTGACGTGCCTGTGCAGCGACGTCTGCCTGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTACCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTg                                                                                                                                                                                                                                 <  2:62907/300‑1 (MQ=255)
                                                                             ttttTCGTTGTCCAGCGACTGACGTGCCTGGGCGGCGACGTCTGCCGGTACTTCGCCCGTGTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTg                                                                                                                                                                                                                           <  1:478049/301‑1 (MQ=255)
                                                                                        ccAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc                                                                                                                                                                                                                <  2:5659/301‑1 (MQ=255)
                                                                                                 tcgtgtgctcttccgatctgTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGcgcg                                                                                                                                                                                                       <  2:391867/282‑1 (MQ=255)
                                                                                                    gTGCTTGTGCAGCGTCGTCTGCCGGTACTTCTCCCGTTTTCGGATTTACCTGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGttt                                                                                                                                                                                                    <  1:423795/301‑1 (MQ=255)
                                                                                                      gCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGAGttttt                                                                                                                                                                                                  >  2:483065/1‑300 (MQ=255)
                                                                                                       tctttccctacacgactctcttccgatctcccGTTGTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg                                                                                                                                                                                                 <  1:304624/272‑1 (MQ=255)
                                                                                                             cAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGc                                                                                                                                                                                           >  2:179934/1‑301 (MQ=255)
                                                                                                                    gTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGTCCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGACAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCCATGGcgcgagatcggaagagcgtcgtg                                                                                                                                                                                    >  1:391353/1‑282 (MQ=255)
                                                                                                                     tCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTgg                                                                                                                                                                                   >  2:211162/1‑301 (MQ=255)
                                                                                                                         ccGGTATTTCTGTCGTTTTCGGATTTACCGGGTTCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCGCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGa                                                                                                                                                                               <  1:482464/301‑1 (MQ=255)
                                                                                                                            gTACTTTGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAc                                                                                                                                                                            <  1:119180/301‑1 (MQ=255)
                                                                                                                                   gCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCg                                                                                                                                                                     >  2:488715/1‑301 (MQ=255)
                                                                                                                                    cccGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCgagatcggaagagcacacgtctgaactcca                                                                                                                                                                    >  2:305028/1‑272 (MQ=255)
                                                                                                                                    cccGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg                                                                                                                                                                    >  1:85623/1‑301 (MQ=255)
                                                                                                                                                       ttaTCTGATCGGAGGTGATTATCATATTTCCCAGTTCAACGCCCTGTACGTAAGTACCGATAGCTGCCGGTGCATTTTCCGTCGCGATTGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCt                                                                                                                                                 <  2:123112/299‑1 (MQ=255)
                                                                                                                                                           cttaCCGGTGGTGTTGATCATATTGCCCAGATTAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGGCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATAGCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTc                                                                                                                                             <  1:511203/298‑1 (MQ=255)
                                                                                                                                                                ggggggTGATTATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTGTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCa                                                                                                                                        <  1:383286/297‑1 (MQ=255)
                                                                                                                                                                 ggAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGCTAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTACTGTTCGGGCAg                                                                                                                                       >  1:539587/1‑301 (MQ=255)
                                                                                                                                                                         tgatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGAAGACCAgaaa                                                                                                                               >  2:396916/1‑298 (MQ=255)
                                                                                                                                                                           ttCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACAt                                                                                                                             <  2:85747/300‑1 (MQ=255)
                                                                                                                                                                           atCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATAAAGTTGCTGTTCGGGCAGACCAGcacaa                                                                                                                             >  2:237278/1‑300 (MQ=255)
                                                                                                                                                                              atatTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTACCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACCTTGt                                                                                                                          >  1:90814/1‑301 (MQ=255)
                                                                                                                                                                               tatTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTACCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTc                                                                                                                         <  2:90938/301‑1 (MQ=255)
                                                                                                                                                                                           taaCGCCCTTTACGTAAGGACCGATAGCTGCCGTTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGTGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCgg                                                                                                             <  2:273215/300‑1 (MQ=255)
                                                                                                                                                                                           cAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCgg                                                                                                             >  2:175869/1‑301 (MQ=255)
                                                                                                                                                                                           aaaCGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCgg                                                                                                             <  1:210919/300‑1 (MQ=255)
                                                                                                                                                                                                                           ggTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCaa                                                                             >  2:533621/1‑300 (MQ=255)
                                                                                                                                                                                                                               cATTTTCCGTCGCGATAGTTTTGCTCATGATTTCCCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGtt                                                                         >  2:299076/1‑301 (MQ=255)
                                                                                                                                                                                                                                  tttCCGTCTCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTc                                                                      <  1:532948/301‑1 (MQ=255)
                                                                                                                                                                                                                                         cgcgATAGTTTTGCTCAAGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAAACTTGGGCGCGATTTACCCAACACTTCATAGTTGTCGATACgg                                                               >  1:457338/1‑301 (MQ=255)
                                                                                                                                                                                                                                           ggttAGTTTTTCTCATGATTTCTCCGTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTAGTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGtt                                                             <  1:559762/298‑1 (MQ=255)
                                                                                                                                                                                                                                                      cTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACATTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCtt                                                  >  2:275039/1‑301 (MQ=255)
                                                                                                                                                                                                                                                      cTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCtt                                                  >  2:569977/1‑301 (MQ=255)
                                                                                                                                                                                                                                                        cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTATCGATACGGTTAACCGTGGCTTgc                                                >  1:378759/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                       ccgtttgttaataaaaGTTTGGCTTCCTATATTGTGCCAGATTTTTATTACCAACCGCAATTAATTGGACAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGcc                                 <  1:612746/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aGCGGTAAAAAGTCTGGTTCATTATAGGGGTCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACAGTATGTGAAAACTCTTTTTCACATTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTg                            <  1:274736/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gtaataaaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAgatcgg                          >  2:613604/6‑296 (MQ=255)
                                                                                                                                                                                                                                                                                   aaaaGTCTGGCTCTTTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGGGGCGCATACAATGCCAGTTGATCTACt                     <  2:308313/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    aaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTAGCTTGCGGCCCATACAATACCAGttcagaacatt                    >  1:360243/1‑292 (MQ=255)
                                                                                                                                                                                                                                                                                    aaaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCCGCACATTGTCGATGCGCTCCGGCCGCCTTGGGCACGATTTACCCACCACTTCACAGGTGTCGATACGGTTAAACGTGGCTTGCGGCCCATACCATCCCCGTTGATCTCCtt                    >  1:117316/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                     aaGTTTGGGTCATTATAGGGGGCCAGACTTTTATTACCAATCGCAATTAATTGGCCAGCACCACATTATGGGAATACTCTGTTTCACAGTAGTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCGGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTg                   <  1:569263/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           ggttCATTTAAGGGAGTCAGACTTTTATTACCAACAGAAATTAATTGGCCAGAACCACATTATGGGAAAACGCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTGCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTc             <  1:175672/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           ggCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATATACTTGATCtta             >  2:590269/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                            gCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCACATACAATGCCAGTTAAACTACTTAATCTTct            >  1:446223/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                               cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTc         >  2:383164/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                 ttATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCaa       >  2:414083/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                      gggAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAATTGAACTACTTGCACTTCACTCAAAAAgg  >  1:63456/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                           
AAGTCGTTCAGATCTTTTACAAACACGGTAGTTTTAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGG  >  NC_000913/4470663‑4471257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑