Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,523,472	A→C	5.5%	F208C (TTC→TGC)	yjhG ←	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,523,472	0	A	C	5.5%	235.0 / 4.3	78	F208C (TTC→TGC)	yjhG	putative dehydratase
Reads supporting (aligned to +/- strand):  ref base A (43/29);  new base C (2/3);  total (45/33)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

CGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGCCCGTCACAAGGATCGGAGACGTACACGGCATAG  >  NC_000913/4523176‑4523765                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      cGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCCCCTGAGATGTCCCGGCAGTGCCCAACAATTGACAGCCACCGCCGGAAGAGGCCCCGGCTTTACAGCCCGCACGGCGCCCGACCCGCACAGATAACACCCCATTGGCGacgac                                                                                                                                                                                                                                                                                                   >  1:130548/1‑297 (MQ=255)     cgcgGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTgcg                                                                                                                                                                                                                                                                                               <  2:407790/301‑1 (MQ=255)           ttGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGAAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATg                                                                                                                                                                                                                                                                                         <  2:431013/300‑1 (MQ=255)             ggACCGTTGTCGCTGTTTCTTTCGTTTTATCGGTGGGAATTTCCCGGGTGGTGATGCCTTTGTGACTCAGGTTCGGGGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATggc                                                                                                                                                                                                                                                                                       <  1:68460/301‑2 (MQ=255)             ggACCGTCATCGCATTTTCTTTCGCTTTATCGGTGGGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGGGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATggc                                                                                                                                                                                                                                                                                       <  1:146239/301‑2 (MQ=255)             ggACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATggc                                                                                                                                                                                                                                                                                       <  2:487119/301‑2 (MQ=255)              gACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGtt                                                                                                                                                                                                                                                                                      >  2:315262/1‑301 (MQ=255)                ccGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGCCAGCCGCCGCCGGAAGAGGCCCAGGCTTTACAGCCCGCCCGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCATGCGCCAATGGtttt                                                                                                                                                                                                                                                                                    >  1:103639/1‑300 (MQ=255)                  gTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCa                                                                                                                                                                                                                                                                                  >  2:501897/1‑301 (MQ=255)                    cctttaccgacgctcttccgttctggTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAcc                                                                                                                                                                                                                                                                                <  1:413516/277‑1 (MQ=255)                        gCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAACTTGc                                                                                                                                                                                                                                                                            >  2:69152/1‑301 (MQ=255)                            tctcTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGtt                                                                                                                                                                                                                                                                        >  2:97227/1‑301 (MQ=255)                             ctctTTCGCTTTGTCGGTGTGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGTCGATCTCCCGCCACCCAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTg                                                                                                                                                                                                                                                                       <  1:97103/301‑1 (MQ=255)                                    gCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTTCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATAGTTTGCACCTTGCCATTGTCTtctc                                                                                                                                                                                                                                                                >  2:286529/1‑301 (MQ=255)                                            ggTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAccagatcggaagagcacaagactgaa                                                                                                                                                                                                                                                        >  2:414079/1‑277 (MQ=255)                                                 gaATTTCCCGGGTGTTGATGCCTTTTTTACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCggg                                                                                                                                                                                                                                                   <  2:357996/301‑1 (MQ=255)                                                                tgATGCCTTTTTGACTCAGGTTCAGCGCAGCCCGCGCGGAAGCTCTGGCGATCTCCCGTCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCCAAAATTGACAGCCGCCGCCGGCAGAGGCACAGCATTTACAGCCCGCCCGGCGTGCGTCCTGTCACGCTAACTCGCCATTGGCGAAGCGTGCGCCACTGGTCTGCACCTTGCCGTTGTCTTCTCCATCCTTTTCCGGCCACGTCGAcccgcc                                                                                                                                                                                                                                    >  1:592878/1‑301 (MQ=255)                                                                    gCCTTTTTGAATCTGTTTCTGTGTGGCTGGCGCGGAAGTTCTGGCGAGCTCCCGCCACACAGGCTCACCGGAAGGGGCGAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCgggg                                                                                                                                                                                                                                <  1:197466/301‑1 (MQ=255)                                                                       ccgatctcTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTATGCGGGCAGCGTCGCGCCGCCGGGGAcc                                                                                                                                                                                                                             <  2:8296/294‑1 (MQ=255)                                                                            aaCTCAGATTCAGCGCAGCTCGCGCGGAATCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAACAATTGACAGCCGCCGCCGCAAGAGGCACAGGCTTTCCAGCCCGCACGGCGTGCGCCCTGTAGCGAAAATTCGCCATTGGCGACACGTCCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCAACCTTTGCAGGCAACGTCGcccccacggatcacagccc                                                                                                                                                                                                                        >  1:242270/2‑286 (MQ=255)                                                                              cTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCCACCTATGCGGGCAGCGTCCACCCCCCGGGGGCCAggtcgg                                                                                                                                                                                                                      >  1:8296/1‑296 (MQ=255)                                                                                cAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCCCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCCGCCACCGTCCCGCCGCCGGGGCCCAGCACGGtt                                                                                                                                                                                                                    >  1:499851/1‑301 (MQ=255)                                                                                         ggcAGCTCGGGCGGAAGCGCGGGCGATCTCCCGCAGCCCAGGTTCACCGGAAGGGGCCAGGGCTTAATGTGGGATTGCCAGTCCCACTCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGAtgttg                                                                                                                                                                                                           <  1:501250/300‑1 (MQ=255)                                                                                                       aaGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAg                                                                                                                                                                                             <  2:130672/301‑1 (MQ=255)                                                                                                               gggtgctcCTGCCACTCTGGTTCACCGGAAGTGGCCAGGGCTTAATGTGGGATGGCCAGTCCCATGCCTGCGGCCACCACCTGGGAGGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCcatca                                                                                                                                                                                     <  1:22057/296‑1 (MQ=255)                                                                                                                  aTCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGTGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCAtgg                                                                                                                                                                                  >  2:638958/1‑301 (MQ=255)                                                                                                                      cccGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCCGCGTCGCGCCGCCGGGGACCAGCACGGTTGCCATGTTGAGCTTCGCGGCGAAGGCCATCACGGTGGc                                                                                                                                                                              >  1:22598/1‑301 (MQ=255)                                                                                                                            ccacaGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAg                                                                                                                                                                        <  2:103763/300‑1 (MQ=255)                                                                                                                                gggttCACCGGAGGGGGCAAGGGCTGAATGTGGGATGGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGcccc                                                                                                                                                                    <  1:69062/297‑1 (MQ=255)                                                                                                                                  gCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCtt                                                                                                                                                                  >  2:475110/1‑301 (MQ=255)                                                                                                                                  cccctacacgacgctcttccgatctaTGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCtt                                                                                                                                                                  <  1:599710/276‑1 (MQ=255)                                                                                                                                   ttCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCGTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTGGCCGTTGTCTTCTCCATCCTTGGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTa                                                                                                                                                                 <  2:593639/300‑1 (MQ=255)                                                                                                                                    acACCGGAGGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGGGAGGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTAt                                                                                                                                                                <  1:474509/300‑1 (MQ=255)                                                                                                                                       ccGGAAGGGGCCAGGGCTGAATTTGGGATTTCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGc                                                                                                                                                             <  2:215924/301‑1 (MQ=255)                                                                                                                                                        tGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCCCGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGTTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATa                                                                                                                                            >  2:306290/1‑301 (MQ=255)                                                                                                                                                           aTGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTagatcggaagagcacacgtctgaaa                                                                                                                                         >  2:600471/1‑277 (MQ=255)                                                                                                                                                                    tGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCCGCGTCGCGCCCCCGGGGCCCAGCCCGGTTCCGATGTTGTGCTGCGCGGCGCGTGCCCTCATGGTGCCCGGACGCCCCTTATCCACACTCCACACACCAAACACCGCCTTcgcc                                                                                                                                >  1:586104/1‑300 (MQ=255)                                                                                                                                                                     gCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGt                                                                                                                               >  2:426735/1‑301 (MQ=255)                                                                                                                                                                     gCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGACACACCAATAACTGCTTTCGCGt                                                                                                                               >  2:643590/1‑301 (MQ=255)                                                                                                                                                                                 ccTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCagagagg                                                                                                                   >  2:473216/1‑300 (MQ=255)                                                                                                                                                                                  ctggacgttcttccgattttGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCg                                                                                                                  <  1:62188/282‑1 (MQ=255)                                                                                                                                                                                       gCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATaa                                                                                                             >  2:555963/1‑301 (MQ=255)                                                                                                                                                                                            caccTGGGATGTCCGGGCAGTGGCCAAAAATTGACAGCCGCGGCCGGAAGAGGCACAGGCTTTACAGCCCGCAGGGCGGGCGGCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGGGCCAATGGTTTGCACCTGGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAggcgg                                                                                                        <  1:314858/301‑1 (MQ=255)                                                                                                                                                                                                     tGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGagatcggaagagcacacga                                                                                               >  2:62256/1‑283 (MQ=255)                                                                                                                                                                                                      gTCCCGGCAGTGCCCACAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCCCGGCGTGCGTCCTGCAGCGATAATTCGCCATTGGCGACGCGTGCGCCAATGGCTTGCACCTTGCCGTTGTCTTCCCCATCCTTTGCGGGCAGCGTCGCCCCGCCGTGGACCAGCACGGTTGCGTTCTTTTGCTTCGCGGCGAGTGCCATCATGGTGGACGGCAGCCCCTTCTCGCCACTGGCCACCCCCATCAATGCTTACGCGTCGGGACGAGCCAGAAAACGGAGGCGCATCAccc                                                                                              >  1:508794/1‑300 (MQ=255)                                                                                                                                                                                                       tCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCCGCGTCGCGCCGCCGGGGACCAGCACGGTTTCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGCAGCCCCTTATCGCAACTCGCCCACCCACTAACTTCTTTCACGTCGGCCACCGAGCCACACAGGCCGCACCTTACCAt                                                                                             >  1:129473/1‑301 (MQ=255)                                                                                                                                                                                                        cccGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCCTCCTGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCACTAACTGCTTTCCCGTCGGGCAGAGAGCGAATAAGGCGGCACATGAcccac                                                                                            >  1:406913/1‑298 (MQ=255)                                                                                                                                                                                                         ccGGGAGGGCCCAAAAAGTGCCAGCCGCCGTCGGGAGAGGCACAGGCTTTACAGCCCGCACGGCGTGTGTCTGGTGGAGTTAATTCGCCATTGGCGAAGCGGGGGCCAATGGTTTGCACCTTGCGGTTGTCTGCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCg                                                                                           <  1:638081/301‑1 (MQ=255)                                                                                                                                                                                                          cGGCAGTGCCCAAAAATTTACAGCCGCCGCCGGAAGAGGCACAGGCTTTCCAGCCCGCACGGTGTGCGTCCTGTAGAGATAATTCGCCAGTGGCGAAGCGTGCGCCAATGGTTTGCACCTGGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCTGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGa                                                                                          <  2:509466/301‑1 (MQ=255)                                                                                                                                                                                                          cGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCAACGa                                                                                          >  2:11798/1‑301 (MQ=255)                                                                                                                                                                                                                   ccAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCAtt                                                                                 >  2:202223/1‑301 (MQ=255)                                                                                                                                                                                                                     gtgtgctcttccgatctgccgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTc                                                                               <  2:328157/284‑1 (MQ=255)                                                                                                                                                                                                                       aaaTTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCgg                                                                             <  2:423522/301‑1 (MQ=255)                                                                                                                                                                                                                        agttgacggcggtgtgcggaggggggagaGGTTTTACAGCCCGCAGGGCGTGGGTTCGGTAGAGAGAATTCGCCATTGGCGAAGCGGGGGCCAATGGTTTGCACCTTGCCGTGGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGt                                                                            <  1:426171/273‑1 (MQ=255)                                                                                                                                                                                                                         atgtctaggcgccgccgGAAGGGGGATGGGCTTTACGGCCGGTAGGGCGTTGGTTCTGGTGTGATGTTTGGTCGTGGGCTGAGCGTGGGGCAAGGGTTTGGACGTGGCCGTTGGCTTCTCCATGCTTGGCGGGCAGCGTCGCGGCGTGGGGGGCCAGCACGGTTGCGATGTTGTGCTGCGAGGCGAGTGCCATCATGGTGGGCGGAAGCCCCTTATCGGATCTCGCGACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTa                                                                           <  1:366959/292‑1 (MQ=255)                                                                                                                                                                                                                                      gccgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTCTCGCAACCCGCCACACCAATAACTCCTTTCCCGTCGGGCCGAGAGCGAAAAAACCGGCGCATTCCCATCGTGGCGTCACTTccgatccgaagggccgcc                                                              >  1:327753/1‑284 (MQ=255)                                                                                                                                                                                                                                         ggAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAAc                                                           <  2:407476/301‑1 (MQ=255)                                                                                                                                                                                                                                             gagGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGCGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGAAGCGAACCAAACATcc                                                       >  1:38201/1‑301 (MQ=255)                                                                                                                                                                                                                                              gggCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATccc                                                      <  2:500498/300‑1 (MQ=255)                                                                                                                                                                                                                                               ggCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGAATTACCATAGAAGCGTCATTTCGGTATGGTAGCGAATCAAACaaaccc                                                     >  2:386593/1‑296 (MQ=255)                                                                                                                                                                                                                                                 cacaGGCTTTACAGCCCGCACGGCGTGCGTTCTGTAGAGATAATTCGCCATTGGCGAAGCGTTCGCCAATGGTTTGCTCCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGt                                                   <  2:601550/301‑1 (MQ=255)                                                                                                                                                                                                                                                 cacaGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATCATTCGCCCTTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGCGCCCAGCCCGGTTGCGATGTTATGCTGCGCGGCGAGTGCCCTCATGCTGGCCCGCACCCCCTTCTCGCACCTCGCCACACCACTAACTGCTTTCCCGTCCGGCCGACACCAACTCACGCCGCGCCTTACCATCGAACCGTCATTTCGGTATgtccgcgcacccaacccccccgc                                                   >  1:361324/1‑279 (MQ=255)                                                                                                                                                                                                                                                 cacaGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGACGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCGCGGTTGCGATGTTGTGCTGCTCTGCGAGTGCCATCATGGTACCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTACTTTCGCGTCCGCCAGACACCGAATAAGGCGGCCCATTCCCATCCATTCATACTTTCGGTATGATACCGACCAAAACATcccctt                                                   >  1:600789/1‑299 (MQ=255)                                                                                                                                                                                                                                                        tttACAGCCCGAACGGCGTGCGTCCTGTAGAGATAATTCGCAATTGGCGAAGCGTGCGCAAATGGTTTGCACCTGGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTAccc                                            <  2:129597/301‑1 (MQ=255)                                                                                                                                                                                                                                                          tACAGGACGCACGCCGTGCGGGCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTg                                          >  2:267979/1‑301 (MQ=255)                                                                                                                                                                                                                                                                ccGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGCTGTCGTGCTGCGCGGCGACAGCCATCATGGTGGCCGGACGCCCCTTCTCGCAACTCGCAACACCAATAACTGATTTCACGTCGGGCCGAGAGCGAATAAGGCAGCGCATTACCATCAATGCGTCATTTCAGTATGGTACCGAATCAAACATCCCCGTTGTACCCTGacaccc                                    >  1:21810/1‑296 (MQ=255)                                                                                                                                                                                                                                                                  gCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCCTTGACCCCTGAGTACGcc                                  >  1:433858/1‑301 (MQ=255)                                                                                                                                                                                                                                                                   cACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGccc                                 >  2:361055/1‑301 (MQ=255)                                                                                                                                                                                                                                                                      ggtgcaaaccattggcgcacgcttCGCCAATGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGCCCGta                              >  2:357900/23‑300 (MQ=255)                                                                                                                                                                                                                                                                         gtgcgtCCTGTAGATATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGCCCGTcaca                           <  2:434422/301‑1 (MQ=255)                                                                                                                                                                                                                                                                         gtgcgtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCCCACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATAGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCAGTTGTACCATAAATACACCCGTaaca                           >  2:321814/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                 tGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGCTGCGTCATTTCGGTATGGTAGCGAATCAAACAACCCCGTTGTCCCCTCAGACCGCCCCTCCCAAgggacgg                   >  1:6121/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                 tGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGCCCGTCACAAGGATCgg                   >  2:562541/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                   tAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGCCCGTCACAAGGATCGgag                 <  2:38253/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                   tAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGCCCGTCACAAGGATCGgaa                 >  2:276996/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         tAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGATTACGCCCGTCACAAGGATCGGAGACGTac           >  1:184012/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                           aTTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGCCCGTCACAAGGATCGGAGACGTacaa         >  2:136729/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                ccATTGGCGCACGCTTCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCCTCGATGCGTCAGTTCGGTATGGTAGCGAAACAAAACACCACGTTGTACCCTGAGTACGCCCGGCAAAAGAAACGGCCACGTACACGGCac    >  1:402052/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  aTTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGCCCGTCACAAGGAACGGAGACgagatcggaagaa  >  2:606136/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      CGAACGCGGCATGGACCGTCATCGCATTCTCTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCACACCAATAACTGCTTTCGCGTCGGGCAGAGAGCGAATAAGGCGGCGCATTACCATCGATGCGTCATTTCGGTATGGTAGCGAATCAAACATCCCCGTTGTACCCTGAGTACGCCCGTCACAAGGATCGGAGACGTACACGGCATAG  >  NC_000913/4523176‑4523765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑