BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,356,852	A→T	W87R (TGG→AGG)	sapA ←	antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,356,852	0	A	T	100.0%	240.1 / NA	76	W87R (TGG→AGG)	sapA	antimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (38/38); total (38/38)

ACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACT  >  NC_000913/1356570‑1357147

acaAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGTGATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTTCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCgg                                                                                                                                                                                                                                                                                        <  2:158871/301‑1 (MQ=255)

  aaaaaaGGAGCACGAGGCTGGGCGAGTCTGAAGTCGACGGTATGATTATCCAGTTTGCGGAGGCTTTTAGCGTTATCGGCAAATTGCAGAGTGTCGAAGGAGGGGAAGTGGCTGCCGTTGACGTTATGCCACGGGGTGTTGCGGTCAAAAATTCGCGGGAAGGTAAACCCCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGTCGC‑AGGTGGAATCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCa                                                                                                                                                                                                                                                                                      <  2:492870/301‑1 (MQ=255)

   aaaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCAt                                                                                                                                                                                                                                                                                     >  1:372163/1‑301 (MQ=255)

   aaaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCAt                                                                                                                                                                                                                                                                                     <  1:249101/301‑1 (MQ=255)

   aaaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCAt                                                                                                                                                                                                                                                                                     >  1:526580/1‑301 (MQ=255)

   aaaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCAACGC‑AGGTGGAAGAGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTTCTTTCGGCAAGTTCCGGctt                                                                                                                                                                                                                                                                                     >  2:459794/1‑299 (MQ=255)

     aaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGTAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTCTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTCCCTCCCTGCTTTCGGCAAGTTCCGGCATCa                                                                                                                                                                                                                                                                                   >  2:117541/1‑301 (MQ=255)

                 ggCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGta‑t                                                                                                                                                                                                                                                                      >  1:30756/1‑301 (MQ=255)

                    tGGGGGAGTCTGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAgg                                                                                                                                                                                                                                                                   <  2:617885/301‑1 (MQ=255)

                      ggCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAgggg                                                                                                                                                                                                                                                                 >  1:403971/1‑300 (MQ=255)

                         agttCGGAACTAAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCAGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATc                                                                                                                                                                                                                                                               <  2:372161/297‑1 (MQ=255)

                          cgTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCg                                                                                                                                                                                                                                                              <  2:526574/299‑1 (MQ=255)

                             cGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGAC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATGGGTA‑TAGGGATCGActt                                                                                                                                                                                                                                                          >  1:130155/1‑299 (MQ=255)

                                       cGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCg                                                                                                                                                                                                                                                <  1:459798/301‑1 (MQ=255)

                                         gTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGAt                                                                                                                                                                                                                                              >  1:74131/1‑301 (MQ=255)

                                         cacgacgctcttccgatctggACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGAt                                                                                                                                                                                                                                              <  2:561025/282‑1 (MQ=255)

                                              aTTAGCCAGTTTGCGGACGCGTAGAGCGTTAGCGGCAAATTGCAGGCGGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATGCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑TGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATaa                                                                                                                                                                                                                                         <  2:292864/301‑1 (MQ=255)

                                                 aTCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑CGGTGGAAGCGATAGGTC‑GTGCCGTTGTCGAGTCCTTCCCTGCCTTCGGCCAGGTCCGGCACTAGGTGAT‑GGTACTAGTGATCGACATCCAGCATTTGATGACaaaa                                                                                                                                                                                                                                       >  1:481616/1‑300 (MQ=255)

                                                 aTCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTCGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGAATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATATGTA‑TTGGGCTCGACCTCCAGCAGTCGATCATaaaa                                                                                                                                                                                                                                       >  2:491332/1‑300 (MQ=255)

                                                       acgacgctcgtccgatctgTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGggcg                                                                                                                                                                                                                                <  2:128430/283‑1 (MQ=255)

                                                        ttGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGggcgg                                                                                                                                                                                                                               >  1:448380/1‑301 (MQ=255)

                                                            ggACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGAtagatcggaagagcacacgt                                                                                                                                                                                                                           >  1:561034/1‑282 (MQ=255)

                                                               cGCTTTTCACGTTAACGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGTTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGTAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATCTGTT‑GCGCTGTTGTCGAGTACTTACCTGCTTTCGGCGAGTTCCGCCACCAGCCGATAGGGA‑TAGGGTTCGACATCCAGCAGTCGATCATAAACCTGGTGGGCAAGGGt                                                                                                                                                                                                                        >  2:344784/1‑301 (MQ=255)

                                                                 cTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGAAAGGTC‑CCGCCGTTTTCGATTACTTCCCTGCTTTCGGCAAGTCCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGAGGCAAGGGttt                                                                                                                                                                                                                      >  2:653567/1‑299 (MQ=255)

                                                                  ttttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGGGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGTTATCTGGTGATAGGTT‑TAGGAATCGACATCCATACGTCGATCGTCAAACTGGCCTGCAAGGGtctt                                                                                                                                                                                                                     >  2:530599/1‑298 (MQ=255)

                                                                  ttttCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATc                                                                                                                                                                                                                     <  1:62680/301‑1 (MQ=255)

                                                                         gTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGggcgagatcggaagagcacacg                                                                                                                                                                                                              >  1:128430/1‑283 (MQ=255)

                                                                             tCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGGA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTAGCGACAATTAAc                                                                                                                                                                                                           >  2:196313/1‑300 (MQ=255)

                                                                               ggCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTT‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGAAAGTTCCGGCATCAGGCGCTAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAACTAAcca                                                                                                                                                                                                         >  2:554974/1‑299 (MQ=255)

                                                                                    aTTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTCTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGc                                                                                                                                                                                                    >  1:127960/1‑300 (MQ=255)

                                                                                      tGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACT‑GGCGGCCAGGGTTTTGACAAATTACCCACTGCTCg                                                                                                                                                                                                 >  1:425506/1‑300 (MQ=255)

                                                                                             tGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTggg                                                                                                                                                                                          >  1:565377/1‑300 (MQ=255)

                                                                                              gTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCACGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTGTGGAt                                                                                                                                                                                         >  2:512089/1‑301 (MQ=255)

                                                                                               tCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATg                                                                                                                                                                                        <  1:606788/301‑1 (MQ=255)

                                                                                                  aaGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCTAAAATTCACTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGTAATCCAGGCGGTTTTTAGAAACGGAACATCGCGACGC‑AGTTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATggg                                                                                                                                                                                      <  1:344785/300‑1 (MQ=255)

                                                                                                  aaGTAGGGGAAGTTGCGGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATggg                                                                                                                                                                                      <  1:554983/300‑1 (MQ=255)

                                                                                                     tAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTa                                                                                                                                                                                   <  1:642278/300‑1 (MQ=255)

                                                                                                     tAGGGGAAGTTGCTGCCGTTGACGGTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGtt                                                                                                                                                                                    <  1:54166/299‑1 (MQ=255)

                                                                                                      aGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGGTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTaa                                                                                                                                                                                  <  2:565368/300‑1 (MQ=255)

                                                                                                              ttGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTg                                                                                                                                                                          <  2:74131/300‑1 (MQ=255)

                                                                                                                 ctgcCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACt                                                                                                                                                                       <  1:101933/300‑1 (MQ=255)

                                                                                                                  tgcCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTTGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCACCGC‑AGGTTGAAGCGATAGGTC‑GCGCCGTTGTCGGGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTg                                                                                                                                                                     <  2:448376/301‑1 (MQ=255)

                                                                                                                           cGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTTGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTTACTTGCCCGCTGa                                                                                                                                                             >  2:41051/1‑300 (MQ=255)

                                                                                                                             ttATGGCAGGGTTTGTTGCGGTCAACAGTTGGCTGGAAGGTAAACGCCACGTCATCGGCATTCATTTAACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACAGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTAGTTCCCTGCTTTCGGCAAGTTCCGGCATAAGGCGATAGGTA‑TAGGGAGCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTAGCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGGCCGCTGACg                                                                                                                                                           <  2:457879/300‑1 (MQ=255)

                                                                                                                                 gCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACCCAATa                                                                                                                                                      >  1:572063/1‑301 (MQ=255)

                                                                                                                                       ggTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGCATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTTTTGACTTGCCGGCTGACGCAATAGACaaa                                                                                                                                                >  2:321106/1‑301 (MQ=255)

                                                                                                                                           gttgCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCg                                                                                                                                            >  2:638417/1‑301 (MQ=255)

                                                                                                                                                ggTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCg                                                                                                                                            >  1:639759/1‑296 (MQ=255)

                                                                                                                                                   cAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTTGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTcgcg                                                                                                                                    >  1:185434/1‑301 (MQ=255)

                                                                                                                                                    aaaaaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCgg                                                                                                                                   <  1:512095/301‑1 (MQ=255)

                                                                                                                                                     aaaaTTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGACTGACATCCAGCAGTCAATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCggg                                                                                                                                  >  2:554726/1‑300 (MQ=255)

                                                                                                                                                       atctCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGat                                                                                                                                 <  2:236665/297‑1 (MQ=255)

                                                                                                                                                        aTTCGCTGGAAGGTAAACACCACATCGTCGGAATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGatat                                                                                                                               <  1:491337/301‑1 (MQ=255)

                                                                                                                                                           cGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑ATGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGatagat                                                                                                                             >  1:236666/1‑297 (MQ=255)

                                                                                                                                                           cGCTGGAAGGTAAACACCACATCGACGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCAGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAg                                                                                                                            <  1:260046/301‑1 (MQ=255)

                                                                                                                                                                          cccacATCGTCGGCATTCAGTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGAtt                                                                                                             <  2:659474/300‑1 (MQ=255)

                                                                                                                                                                           ccacATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTCTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTc                                                                                                            <  2:127960/301‑1 (MQ=255)

                                                                                                                                                                            cacaTCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCa                                                                                                           <  1:530607/301‑1 (MQ=255)

                                                                                                                                                                                     ggCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCgcggcg                                                                                                  >  2:546524/1‑301 (MQ=255)

                                                                                                                                                                                      gCATTCATTTTACGAGGGGGAGGAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCAGGGGAGATTCAGGCgcggcg                                                                                                  <  2:572054/300‑1 (MQ=255)

                                                                                                                                                                                         ttcattTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTg                                                                                              <  1:117541/301‑1 (MQ=255)

                                                                                                                                                                                            tgtttACGAGTGGAATGAAACCAGTAGGTTTTTTGAAACGGAACATC‑CGGCGCGAGGTGGAAGAG‑TAAGTCGGCGCCGTTGGCGAGTACTTCCCTGCTTCCGGAAAGTTCCGGAATAAGGCGTTGGGTA‑GAGGGAACGAAATCCGGAAGTCGATCATAGAACT‑GGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTg                                                                                          <  2:425503/299‑1 (MQ=255)

                                                                                                                                                                                             ttttACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTCCTCGCTTTGGATGGGTTACAGGTGTTGACTTGCCCGCTGCCGCAATAGACAACACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCt                                                                                           >  2:470358/1‑300 (MQ=255)

                                                                                                                                                                                                    aGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTCGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACt                                                                                    >  2:24606/1‑300 (MQ=255)

                                                                                                                                                                                                       gggAGTATACCAGTCGGCCTTTCGATACGGACCAACTCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGCTGTCGAGTACTTCCCTGCTTTCGGCAATTTCCGGCATCAGGCGTTAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATTAATCTGGGCGGCAAGGGTATTGATATTTAACTCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCTCTGTCGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCCCGGCGATTGCCTGACCACTcaca                                                                                >  2:248866/1‑301 (MQ=255)

                                                                                                                                                                                                               aCCAGTCTGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTACCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAGACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCAATCACAAGTCCAGc                                                                        <  1:321107/301‑1 (MQ=255)

                                                                                                                                                                                                                aacgctcttccgctgtaaCGGAACGTAGGGAAGC‑AGGTTGAAGCGATAGGTT‑GCGACGTTGTCGAGTCATTCCCTGCTTTCGGCAAGTTCCGGCATAAGGCGATAAGTA‑TAGGGATCTACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCGTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGGCAAAACCGCTGTCGCGGAGATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACTAGTCCAGCa                                                                       <  2:356411/285‑1 (MQ=255)

                                                                                                                                                                                                                        ttttttGAAACGGAACATCGGGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCAAGAAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCa                                                                <  2:30756/300‑1 (MQ=255)

                                                                                                                                                                                                                            ttGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAaga                                                           <  1:653578/301‑1 (MQ=255)

                                                                                                                                                                                                                                aaCGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGTGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCAAGCaagatcggaagagcaca                                                       >  1:356412/1‑286 (MQ=255)

                                                                                                                                                                                                                                      aCATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGACAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGataata                                                 <  1:470363/301‑1 (MQ=255)

                                                                                                                                                                                                                                               cGC‑AGGTGGAAGCGATAGGTC‑GCGCCGCTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATTAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAATTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGTGGATATCAGCAGGCGGGGGAGATTCAGGCCCGGCGTTTGCCTGACCACCTCCCAGTCCAGCAATCAACAACAGACCCGATAATActgtgcgc                                         >  2:425195/1‑293 (MQ=255)

                                                                                                                                                                                                                                                              aTAGGTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGAt                          >  1:214014/1‑301 (MQ=255)

                                                                                                                                                                                                                                                                  gTC‑GCGCCGTTGTCGAGTACTTCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCAAAAGTCCAGCAATCACCAAAAGAGACAATAATACCTGGCGCATACTATTAAGGGAttttt                      >  2:28907/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                            ttggggAGTAAGTCCCTGCGTTCGGAAAATTCCGGCATCAGGAGAGAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACGGCTCGCATTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCGGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAagag             <  2:639748/296‑1 (MQ=255)

                                                                                                                                                                                                                                                                                       ttCCCTGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTCGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACt  <  1:24606/301‑1 (MQ=255)

ACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGC‑AGGTGGAAGCGATAGGTC‑GCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTA‑TAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACT  >  NC_000913/1356570‑1357147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`