| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 92.6% | 77.6 / ‑2.0 | 27 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (13/12); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCC > NC_000913/3560161‑3560746 | cccggtgcgatggatAATTAGCCAGCGTGGAGAGTGATGCGACAATCTGCTTTTACCAGCAACGTGTTGGCAACGTTGGGATTGTTTGTGACGATGCGCAAGTTGCTGTGCTTGAGGGTTGGGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGa < 2:305430/287‑1 (MQ=255) tCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTTCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGACAAACAGCGTCGCGCCATTGGGGACTTGCTCCGCCACTCTGCGGGCGATGCGCTCTTTTTCCTCGGTCTGGGTGGCCTCGCGATCGTGCCACGGCGTGTCAACCGAACCGGATGGCAGCACCGCACCGCCCTGAGG‑GCC‑CAGGACATGGTTTTGCTCCGcc > 2:243313/1‑300 (MQ=255) ccGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGTCATTGGGCATTTGCTCCGCCACTTTGCGGGCGATGCCCTCTTTTTCTTCGGTCTTGGTGGCCTTGCTCTCTTCCCACGGCTTGTTAACCGACCTTGATGGCAGCGCCTCACCCCCCTGATGTG‑C‑CAGGCTTCGGTTTTGCTCCTCCacccctt > 2:33358/1‑295 (MQ=255) atgatgCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGCGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCACGGTGCCGATATCGATAACCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTCCGGGCGATGCGCTCTTTTTCTTCGTTCTGGGTGGCCTTGCGTTATTGCTACTGCGTGTCAACCGAACTTGGCAGCAGACACACAACGCCATGATG‑G‑CGCCGGATCAGGTTCTCCTCCGCCAACTCTTTCTGGTct > 1:253823/1‑299 (MQ=255) aaCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCGCCGCCAGCCCATTGCGGTCGCGGCGAATGGGCTGCGGGCTGAGGgaga > 2:286090/1‑301 (MQ=255) ggtgacatgggggaaggtGCTGTGAGTGAGAGCTCCTTGGGGTACGGCTTCCGGGGGGGGGCCGTTATCGATGAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTGTCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt < 2:191431/283‑1 (MQ=255) ggTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCCCCGCACCGCCATGATG‑G‑CGCAGGATCAGTTTTTGCTCCGACAGCTCATTGAGTTCGCGGAGAATCGTCTGGGGGATGGCGGAGAAATGCTCTACCactccttggttactt > 2:194448/1‑287 (MQ=255) gACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa > 1:198921/1‑301 (MQ=255) cAATGCGCAAATTGCTGGGATTGAGCAGTGCGTGCGCTGCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCCTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa < 1:243324/301‑1 (MQ=255) cAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg > 1:60176/1‑300 (MQ=255) tgatgcggCAGTGCGTGCGCTGTCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCTTCGAGCCTTTGGTGGTTTGCTCCGCCACTTTGCGGACGATGCTCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAGcc < 1:215713/293‑1 (MQ=255) gCAGTGCGTGCGCTACCGCTTCCGGCGTGGCGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATAAGGTTTTGCTCCGCCACCTCATCGAGGTCGCGGCGAATAGTCTCCGGGCTGACGGGGAAATGCCCTCCCAGCTCCTCGCTACGGACCTAACCctgttaaatcggaagagcca > 1:256784/1‑283 (MQ=255) gcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGTGATCGTGCCACGGCGTGTCAACCGAACTGGAAGGCTGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTCTTGCTCTGCCCGCTCCTTGAGGCCCCGGCGAATAGCCTGCGGGCTGACGGAGAAACGCGCTAACAGCCCGTCGGTAGTGCCATAACCCGGCTGTTTAACAAGCTAGATAACACCGTtgg > 2:55528/1‑300 (MQ=255) ccGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCATGTCGATAATACCGTTTTGa > 1:265099/1‑299 (MQ=255) ccGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt < 1:286102/300‑1 (MQ=255) gATATAGAAAAACAGAGTCGAGCCATGGGGGGTTTGCTCCGCCACTTTTCGGGGTGTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTta < 2:198911/300‑1 (MQ=255) gCGTCGGGCCATTGGGGATTTGCTCCGCAACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAat < 1:194458/300‑1 (MQ=255) tggCCATTGTGGGATTGTCCTGCCACTTGGCGGGCGGTGCGCTATTTTTCTTCGGTGTGGGGGGCCTTTGGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGTGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttt < 2:8885/298‑1 (MQ=255) gAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGCTTGTTTCATTTATAAATCCCTGGAATTAtttt > 1:281432/1‑301 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACAAGTTCGCTAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAATTTCCTGGAATTATTTGCGTTTTcgcg > 1:7183/1‑300 (MQ=255) ttgtgcgtgcgttcggcTCTTTTTCGTAGGTCTGGGTGCCCTTGCGTCAGGGCCCCGGCGTTTTACCCGAACTGCAAGGCAGGGCCGCACCGGCATGATA‑G‑CGGAGGGTCAGGTTTTGCTCCGCCGAGTCGATGAGGTGGCGGCGCATAGTTTACGGGCTGACGGAGAGATGCTCTACCAGCTCTTCGGTTCTGACAGAACCCTGCTGTTTAACCAGTTCGATAATACCGTTTGGGCGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa < 2:281420/286‑1 (MQ=255) tGGGTGGCCTTGCGATCGTGCCACGGCGTATTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg < 1:55531/301‑1 (MQ=255) cGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATTGGTGCTCCGTTCGCCATcta > 1:113546/1‑299 (MQ=255) tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAAAAAAAGCCATCGCTAAACCAACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACa > 2:238525/1‑301 (MQ=255) tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCTTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCTTCTCTAAACCCACGGCNAACCCGGCGATCTTTGCTCCGTTCGCCATCGACATc > 2:47236/1‑300 (MQ=255) ggAAGGCAGGGCCGCACCGCCATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAAt < 1:47239/300‑1 (MQ=255) cATGATG‑G‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTTACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATcc < 1:169458/301‑1 (MQ=255) | CCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATG‑G‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCC > NC_000913/3560161‑3560746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |