Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	50 [0]	[0] 50	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	50 (1.530)	50/592	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TTCGCATCATCAAGCGACAGTTCGGCTTCGTGAAAGCCAGATACAAGGGGTTGCTGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCGGGCAACCGCCAGACGATTCTTTATTGGTAATGAGAATAATTAACAATTAAAGAGCGTCGCGAAAGAATAATGTGTCTCG  >  NC_000913/1300402‑1300990                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                     cgttactggtattaaggggattttccccggcctgcttcattaattcctttcaaatgaaacggagctgccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagatgaaagtgctaaataat                                                                                                                                                                                                                                                                                                  >  1:308117‑M2/297‑301 (MQ=255)          tattaaggggattttccccggcctgcttaattaattcctttcaaatgaaacggagctgccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTg                                                                                                                                                                                                                                                                                         <  1:172400‑M2/14‑1 (MQ=255)            ttaaggggattttccccggcctgcttaattaattcatttcaaatgaaacgtagctgccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttagtttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                                                                                                                                                                       <  2:151208‑M2/16‑1 (MQ=255)             taaggggattttccccggcctgtttaattaattccttttaaatgaaacggagttgccatgttccgtttacttcgtcattatttttactttgttctcgcgcagttatcaaaagcaaaaggaataggtaaaattattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatttttttttcgccacatcataatcttgagcttgctgaagcatcattgtaatgatattattaattcaacttccttagatagaggaaagtgctaaTTAATAATCAATTTTTa                                                                                                                                                                                                                                                                                      >  2:176760‑M2/285‑301 (MQ=255)                     ttttccccggcctgcttaattcattcctttcaaatgaaacggagcttccatgttccgtttacttcgtccttatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtg                                                                                                                                                                                                                                                                              <  2:74226‑M2/25‑1 (MQ=255)                          cccggcctgcttaattaattcctttcaaatgaaacggcgctgccacgctccgtttacttcgtcattatttttactttgttccagcgcagtcatcaaactcaataggaataggtaaaaatattcttctcaatttacagtcagttataaggatttaattaactgcttctcctcatcaccatgttattttcgccacatcataatcctgggcctgtggaagaataattgaaatgatactattaattccactgcagttggtagaggaaggtgctaaATAATAATCAATTTTTaaattgttgttaat                                                                                                                                                                                                                                                                         >  2:195885‑M2/272‑297 (MQ=255)                                     taattaattcctttcaaatgaaacggagctgccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                                                                                                                                                                              >  1:228164‑M2/261‑301 (MQ=255)                                          aattcctttcaaatgaaacggagctgccatcctccgtttccttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataagtatttccttaattgcttctcctcaccatcttgttattttcgccacaccatcatcttgggcctgctgaggaataattgaaatgatattattacttccactgcctttggtcgaggaaagtgcgatATACTAATTAATGCTTAAATTATTGTCCATTTCACTACTGGAActt                                                                                                                                                                                                                                                         >  2:18874‑M2/256‑300 (MQ=255)                                              cctttcaaatgaaacggagctgccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaagtacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgcaacatcataatcctgggcttgctgaagaataattgaaatgatattcttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTTTGCATTTCACTACTGGAACTGTAAt                                                                                                                                                                                                                                                     >  1:190674‑M2/252‑301 (MQ=255)                                                  tcaaatgaaacggagctgccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGa                                                                                                                                                                                                                                                 >  1:135632‑M2/248‑301 (MQ=255)                                                   caaatgaaacggagctgccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTANATTATTGTGCTTTTCACTACTGGAACTTTAATCAGaa                                                                                                                                                                                                                                                >  2:43417‑M2/247‑301 (MQ=255)                                                      atgaaacggagctgccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAg                                                                                                                                                                                                                                             >  1:11088‑M2/244‑301 (MQ=255)                                                        gaaacggagctgccatgccccgtttacttcgtcattatttttactttgttcccgcgcagttttcaaaagcaaaatgcatcggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctctccattatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGTAACTGTAATCAGAAAAGAt                                                                                                                                                                                                                                           >  1:123567‑M2/242‑301 (MQ=255)                                                                 ctgccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagttctaaATAATAATCAATTGTTAACTTATTGTGCATTTCACTACTGTAACTGTAATCATAAACGATAGAAatt                                                                                                                                                                                                                                    >  2:2935‑M2/233‑298 (MQ=255)                                                                   gccatgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGATTa                                                                                                                                                                                                                                >  1:217491‑M2/231‑301 (MQ=255)                                                                      atgctccgtttacttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaactgatattattaattcaactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCATTACTGGAACTGTAATAAGAAAAGATAGCAATGCTTAGc                                                                                                                                                                                                                              >  2:280456‑M2/228‑300 (MQ=255)                                                                      atgctccgtttaattcgtcattaattttactttgttcccgcgcagttatcaaaagcaaaatgaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGcc                                                                                                                                                                                                                             <  2:135623‑M2/74‑1 (MQ=255)                                                                           ccttttacttcgtcattatttttactttgttcccgctcagttataaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtct                                                                                                                                                                                                                        <  1:200199‑M2/79‑1 (MQ=255)                                                                                 acttcgtcattatttttactttgttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTTTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGCCATGATTAGCCAATCTCTAttt                                                                                                                                                                                                                  >  2:255310‑M2/217‑301 (MQ=255)                                                                                      tttattatttttactgtaggcccaccaagttatcaaaagcaaaaggaataagtcaaaatatgattctcaaagtacagtaagttataaggatttccttaactgcttctcctaaccagcatgttatgttcgccacctcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttgttagaagaaagtgctaaATAATAATAAATTGTTAAATTATTGTGCATGTCACGACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattg                                                                                                                                                                                                             <  2:314223‑M2/90‑1 (MQ=255)                                                                                                       tttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                                                                                                                                                                            <  1:182750‑M2/107‑1 (MQ=255)                                                                                                        ttcccgcgcagttatcaaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                                                                                                                                                                           <  1:255321‑M2/108‑1 (MQ=255)                                                                                                                       caaaagcaaaaggaataggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                                                                                                                                                                            >  1:95580‑M2/179‑301 (MQ=255)                                                                                                                                   aaataggtaaaaatattcttcacaaattacagttagttataaggatttccttaacttcttctcctcaccatcagattattttcgccaaatcatagtcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttcgtagaggaaagtgataaAGAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGGTGGACATGCTTAGCCAATCTCTATTTTATTGAATTAAAAGAGGGTTGTTAAGGCATGGATGCAAACTATAGATTCt                                                                                                                                                                <  2:123558‑M2/135‑1 (MQ=255)                                                                                                                                     atatgtaaaaatattcttttcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa                                                                                                                                                              <  2:217481‑M2/137‑1 (MQ=255)                                                                                                                                        ggtaaaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgatgaataattgaaatgatattattaattccactgcctttggcagaggaaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGTTGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa                                                                                                                                                            >  1:42170‑M2/162‑300 (MQ=255)                                                                                                                                            aaaatattcttctccaaatacagttatgtataaggatttccttaactgcttctcctcaccatcaggttattttcgccacatcataatcctaggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTc                                                                                                                                                       <  2:7001‑M2/144‑1 (MQ=255)                                                                                                                                            aaaatattcttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt                                                                                                                                                        >  1:178999‑M2/158‑300 (MQ=255)                                                                                                                                                     ttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGAATGTATGCAAGCTATAGATTCTGATACGGTCAATAAAaga                                                                                                                                              >  1:226022‑M2/149‑301 (MQ=255)                                                                                                                                                      ttttaaattagaggtagttagaaggatttacgtaagtgcttggcctcgccatgaggttatgttcgccacatgataatcctgggcttgctgaaggataattgaaatgatagtattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAagag                                                                                                                                             <  2:11087‑M2/154‑1 (MQ=255)                                                                                                                                                       ctcaaattacagttagttataaggattgccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgccttcggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATCCTTAGCCAATCCATATTTGATTGAATTCAAAGTTGTTTGTTAAGTCATGGATGCAATCTATAGATTCTGATACGGTCAATAAAAgaga                                                                                                                                            >  2:291070‑M2/147‑301 (MQ=255)                                                                                                                                                        tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGaa                                                                                                                                           >  1:313723‑M2/146‑301 (MQ=255)                                                                                                                                                             ttacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCt                                                                                                                                      <  2:228154‑M2/161‑1 (MQ=255)                                                                                                                                                                  gttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGCTGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATATGGTCAATAAAAGAGAATTGCTTAACa                                                                                                                                 >  1:316286‑M2/136‑301 (MQ=255)                                                                                                                                                                                                  ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATATCTATTTGATTGAATTGACAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGATATTAAGAAcc                                                                                                  >  2:22418‑M2/104‑300 (MQ=255)                                                                                                                                                                                                     cccatcatgttattttcgacacagcattatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCAt                                                                                              <  2:313710‑M2/201‑1 (MQ=255)                                                                                                                                                                                                       catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCAttt                                                                                            <  2:178989‑M2/203‑1 (MQ=255)                                                                                                                                                                                                                attttcgccacagcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTc                                                                                   <  2:82094‑M2/212‑1 (MQ=255)                                                                                                                                                                                                                 tttgcgccacatcataatcctgggctttctgaataataattaaaatgatattattaattccactgcctttggtagaggaaagttctaaATAATAATCAATTTATAAATTATTGTGCATTTCACTACTGGAACTATAATCATAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTTAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTcc                                                                                  <  2:190664‑M2/213‑1 (MQ=255)                                                                                                                                                                                                                    ctgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCggg                                                                               <  1:199973‑M2/216‑1 (MQ=255)                                                                                                                                                                                                                      gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGAAAAATGTATTGGCGAGTAAGAAACGAATGTGGTACTTTctgggag                                                                             >  2:199963‑M2/84‑299 (MQ=255)                                                                                                                                                                                                                       ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCGGGCa                                                                             <  1:2935‑M2/218‑1 (MQ=255)                                                                                                                                                                                                                                                  aaataatttaaatgatattattaattccacttactttggtagaagaaaattctaaATATTAATCAATTGTTAAATTATTGTTCATTTCACTACTGTAACTGTAATCAGAAAAAATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATAGATGCAAGCTGTAGATTCTGATACGATCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCGGGCAACCGCCAGACGATTCTTTATTGGTAATg                                                 <  1:195895‑M2/246‑1 (MQ=255)                                                                                                                                                                                                                                                       attgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGCCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTACGGGCAACCGCCAGACGATTCTTTCTTGGTAATGAGaat                                            >  1:193768‑M2/51‑301 (MQ=255)                                                                                                                                                                                                                                                                    tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCGGGCAACCGCCAGACGATTCTTTATTGGTAATGAGAATAATTAACAATTaa                               >  1:279954‑M2/38‑301 (MQ=255)                                                                                                                                                                                                                                                                      tgaattacactgactttggtagaggaaagggctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGGATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCGGGCAACCGCCAGACGATTCTTTATTGGTAATGAGAATAATTAACAATTAAag                             <  1:18875‑M2/266‑1 (MQ=255)                                                                                                                                                                                                                                                                                  cctttggtggaggaaagtgctaaATAATAATCAATTGATAAATTATTGTGCATTTCACTACTAGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCACTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACAGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCGTTTGGTACTTTCCGGGCAACCGCCAGACGATTCTTTATTAGTAATGAGAATAATTAACAATTAAAGAGCGTCGCGaa                  <  1:176770‑M2/277‑1 (MQ=255)                                                                                                                                                                                                                                                                                             ggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCGGGCAACCGCCAGACGATTCTTAATTGGTACTGAGAATAATTAACAATTAAAGAGCGTCGCGACACAATAATgtgt      >  2:220796‑M2/13‑301 (MQ=255)                                                                                                                                                                                                                                                                                              gaaaattctaaATAATAATCAATTGTTAAATTATTGTGCAATTTACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATTTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCGGGCAACCGCCAGACGATTCTTTATTGGTAATGAGAATAATTAACAATTAAAGAGCGTCGCGAAAGAATAAtgtg       <  2:95574‑M2/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCGGGCAACCGCCAGACGATTCTTTATTGGTAATGAGAATAATTAACAATTAAAGAGCGTCGCGAAAGAATAATGTGTCTCg  <  1:280468‑M2/293‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                     TTCGCATCATCAAGCGACAGTTCGGCTTCGTGAAAGCCAGATACAAGGGGTTGCTGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAAAGAGAATTGCTTAACAATTTTGCAAAATGTATTGGCGAGTAAGAACCGCATTTGGTACTTTCCGGGCAACCGCCAGACGATTCTTTATTGGTAATGAGAATAATTAACAATTAAAGAGCGTCGCGAAAGAATAATGTGTCTCG  >  NC_000913/1300402‑1300990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑