Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,464,163	(T)6→5	coding (547/1656 nt)	treC ←	trehalose‑6‑P hydrolase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	4,464,158	0	T→.	100.0%	395.8	90	coding (552/1656 nt)	treC	trehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand):  new base (45/44):  ref base (1/0):  total (46/44)

GTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGC  >  NC_000913/4463967‑4464348                                                                                                                                                                                                |                                                                                                                                                                                               gTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACtttttcagc                                                                                                                                                                                        <  1:104516/200‑5 (MQ=255) gTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACtttttcagc                                                                                                                                                                                        >  1:512816/1‑196 (MQ=255) gTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACtttttcagc                                                                                                                                                                                        >  1:216270/1‑196 (MQ=255) gTCATTAACCCGCGTAGCGTAAACACATCGCGGTTCATCGCGTGCAAAAACTCGTGTTCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACtttttcagc                                                                                                                                                                                        <  1:21676/200‑5 (MQ=255)   cATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGCCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGctc                                                                                                                                                                                     >  1:764595/1‑200 (MQ=255)            gcgTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTAc                                                                                                                                                                            <  1:107805/200‑1 (MQ=255)                 gCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTg                                                                                                                                                                       >  1:652132/1‑200 (MQ=255)                 gCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTg                                                                                                                                                                       >  1:277459/1‑200 (MQ=255)                 gCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTg                                                                                                                                                                       >  1:229444/1‑200 (MQ=255)                 gCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGCAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTg                                                                                                                                                                       >  1:746863/1‑200 (MQ=255)                    tAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGAt                                                                                                                                                                    <  1:170254/200‑1 (MQ=255)                         acaTCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTcc                                                                                                                                                               <  1:190267/200‑1 (MQ=255)                          caTCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTccc                                                                                                                                                              >  1:400511/1‑200 (MQ=255)                            tCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGCGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAg                                                                                                                                                            >  1:742865/1‑200 (MQ=255)                               cgGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTg                                                                                                                                                         >  1:87583/1‑200 (MQ=255)                               cgGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCACGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCCCTGCGCGTACCGCTGGATTCTCCCAGTTg                                                                                                                                                         >  1:409102/1‑200 (MQ=255)                                    cATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATc                                                                                                                                                    <  1:815131/200‑1 (MQ=255)                                             cAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTc                                                                                                                                           <  1:253489/200‑1 (MQ=255)                                             cAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTc                                                                                                                                           <  1:82885/200‑1 (MQ=255)                                                aaaCTCGTGTGCTCGGGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCCGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACtttttc                                                                                                                                                                                           <  2:706412/150‑2 (MQ=255)                                                   cTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACtttttcagc                                                                                                                                                                                        <  2:571336/150‑5 (MQ=255)                                                       agtgATCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAag                                                                                                                                 <  1:819505/199‑1 (MQ=255)                                                           cTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGCTCGGCCCAGAACTCACAGACtctttccg                                                                                                                                                                                         >  4:83708/1‑138 (MQ=255)                                                           cTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTT‑AGCTCTGcgcg                                                                                                                                                                               >  2:280538/1‑150 (MQ=255)                                                           cTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGAt                                                                                                                             >  1:202154/1‑200 (MQ=255)                                                            tCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACtttttcagc                                                                                                                                                                                        <  4:405286/141‑5 (MQ=255)                                                                   ccGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTgcgc                                                                                                                                                                                <  4:496658/141‑1 (MQ=255)                                                                   ccGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAg                                                                                                                     <  1:411836/200‑1 (MQ=255)                                                                      tCGGTGTAGAAGCGACGCCCGTCTCCTTCCAGGTCTTCAGGGCGACGCGGGTCTTTGGAGATCAGATTCGCCACAGCCAGGCGCAACCCGTCGACCCCACGATCGTCCCAGAACTCATAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGAt                                                                                                                                                                    <  2:564216/150‑1 (MQ=255)                                                                      tCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGAt                                                                                                                                                                    <  2:77189/150‑1 (MQ=255)                                                                      tCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGAt                                                                                                                                                                    <  2:516718/150‑1 (MQ=255)                                                                      tCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGACCAGAACTCACAGAC‑TTTTTCAGct                                                                                                                                                                                      >  3:384106/1‑132 (MQ=255)                                                                      gcGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGAt                                                                                                                                                                    <  2:559453/149‑1 (MQ=255)                                                                      gcGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGAt                                                                                                                                                                    <  2:17476/149‑1 (MQ=255)                                                                         gtgtAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAATCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCg                                                                                                                                                                          >  4:245590/1‑141 (MQ=255)                                                                         gtgtAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGACCTCACAGAC‑TTTTTCCGCTCTGCGCGTACCGCTGGATtct                                                                                                                                                                 >  2:158451/1‑150 (MQ=255)                                                                         gtgtAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCTCAGAC‑GTTTTCAGCTCGGCGCGTACCGCTGGATtct                                                                                                                                                                 >  2:753084/1‑150 (MQ=255)                                                                         gtgtAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGt                                                                                                               >  1:194985/1‑200 (MQ=255)                                                                         gtgtAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGt                                                                                                               >  1:285350/1‑200 (MQ=255)                                                                         gtgtAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCAGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCCCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGt                                                                                                               >  1:421428/1‑200 (MQ=255)                                                                         gtgtAGAAGCGACGCCCGTCGCCGCCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGt                                                                                                               >  1:761531/1‑200 (MQ=255)                                                                         gtgtAGAAGCGACGCCCGTCGCCGCCCAGGTCTTCAGGGAAACGCGGGTCTCTGGAGAGCAGATTCACCAAATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGTTCTGCCCGTACCGCTGGATtct                                                                                                                                                                 >  2:706354/1‑150 (MQ=255)                                                                             aGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGc                                                                                                           <  1:396116/200‑1 (MQ=255)                                                                               aaGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGAt                                                                                                                                                                    <  4:43713/141‑1 (MQ=255)                                                                                aGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTCTTCAGCTCTGCGCGTACCGCTGGAtt                                                                                                                                                                   >  4:536961/1‑141 (MQ=255)                                                                                    aCGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTgaga                                                                                                                                                      <  2:509378/150‑1 (MQ=255)                                                                                     cGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTccc                                                                                                                                                              <  4:102336/141‑1 (MQ=255)                                                                                      gCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATc                                                                                                                                                    <  2:442997/150‑1 (MQ=255)                                                                                      gCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCAt                                                                                                  >  1:654443/1‑200 (MQ=255)                                                                                      gCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACAAGTCGACCCCACGATCGGCCCAGCACTCCCAGAC‑GTTTTCAGCTCTGCGCGTACCGCTgg                                                                                                                                                                      >  3:1644/1‑132 (MQ=255)                                                                                      gCCCGTCGCCGTCCAGGTCTGCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATc                                                                                                                                                    <  2:554600/150‑1 (MQ=255)                                                                                        ccGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGAt                                                                                                                                                                    <  3:137599/132‑1 (MQ=255)                                                                                           tCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCt                                                                                                                                               >  2:456988/1‑150 (MQ=255)                                                                                               cGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTc                                                                                                                                           <  2:400463/150‑1 (MQ=255)                                                                                                gTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCt                                                                                                                                          <  2:742797/150‑1 (MQ=255)                                                                                                gTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCa                                                                                        >  1:693443/1‑200 (MQ=255)                                                                                                   cAGGTCTGCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTg                                                                                                                                                         <  3:628562/132‑1 (MQ=255)                                                                                                    aGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTg                                                                                                                                      <  2:683454/150‑1 (MQ=255)                                                                                                     ggTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCCCGTACCGCTGGATTGTCCCAGTTGAGATCCGCCTg                                                                                                                                              >  4:510049/1‑141 (MQ=255)                                                                                                        cTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTAc                                                                                >  1:524780/1‑200 (MQ=255)                                                                                                        cTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGAGTACCGCCGGATTCTCCCAGTTGAGATCCGCCGGTTc                                                                                                                                           >  4:153503/1‑141 (MQ=255)                                                                                                             gggAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTg                                                                                                                                      >  4:406300/1‑141 (MQ=255)                                                                                                             gggAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCa                                                                           >  1:391445/1‑200 (MQ=255)                                                                                                                        gTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTAc                                                                                                                  <  2:174279/150‑1 (MQ=255)                                                                                                                          cTTTGGAGATCCGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCaa                                                                                                                         <  4:283409/141‑1 (MQ=255)                                                                                                                          cTTTGGAGATCAGATTCACCACATCCGGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCaa                                                                                                                         <  4:366326/141‑1 (MQ=255)                                                                                                                            ttGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAaga                                                                                                                                >  3:543170/1‑132 (MQ=255)                                                                                                                            ttGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAAt                                                                                                                       >  4:556250/1‑141 (MQ=255)                                                                                                                                agaTCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTa                                                                                                                   >  4:378814/1‑141 (MQ=255)                                                                                                                                 gaTCAGATTCACCACATCCAGGCGCAACACGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTAc                                                                                                                  <  4:277077/141‑1 (MQ=255)                                                                                                                                    cAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGCTTCCCCCAGTTGAGATCTGCCTGTTCTGTTGCAAAGAGATGCAAATAGTACTGt                                                                                                               >  4:150204/1‑141 (MQ=255)                                                                                                                                      gATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTc                                                                                                             <  4:179978/141‑1 (MQ=255)                                                                                                                                      gATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTg                                                  >  1:760036/1‑200 (MQ=255)                                                                                                                                      gATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTg                                                  >  1:785882/1‑200 (MQ=255)                                                                                                                                      gATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTATCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATTCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTATACGCCAGTGGTTCGGTg                                                  >  1:499663/1‑200 (MQ=255)                                                                                                                                          caccacATCCAGTCGCAACCCGTCGACCCCACGATCGGCCCAGACCTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTAc                                                                                                                  <  3:10773/132‑1 (MQ=255)                                                                                                                                          caccacATCCAGGCGCAACCCGTCGACCCTACGAGCGGCCCAGAACGCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTAc                                                                                                                  <  3:153498/132‑1 (MQ=255)                                                                                                                                          caccacATCCAGGCGCAACCCGTCGACCCCACGATCGGCGCAGAACTCACAGAC‑TTTTACAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTAc                                                                                                                  <  3:504022/132‑1 (MQ=255)                                                                                                                                          caccacATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTAc                                                                                                                  <  3:157478/132‑1 (MQ=255)                                                                                                                                            ccacATCCAGGCGCAACCCGTCGACCCCACGATCGTCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTg                                                                                                                <  3:230082/132‑1 (MQ=255)                                                                                                                                               caTCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGc                                                                                                    >  4:298402/1‑141 (MQ=255)                                                                                                                                               caTCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TCTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTCGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGc                                                                                                    >  4:203229/1‑141 (MQ=255)                                                                                                                                                         cAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAgcgc                                                                                          <  4:543202/141‑1 (MQ=255)                                                                                                                                                         cAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAgcgc                                                                                          <  4:450134/141‑1 (MQ=255)                                                                                                                                                          aaCCCGTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTAc                                                                                <  2:538652/150‑1 (MQ=255)                                                                                                                                                               gTCGACCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCa                                                                           <  2:197271/150‑1 (MQ=255)                                                                                                                                                                  gACCCCACGATCGGCCCAGAACTCCCAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGat                      <  1:815150/200‑1 (MQ=255)                                                                                                                                                                   aCCCGACGATCGTCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTAc                                                                                <  4:252893/141‑1 (MQ=255)                                                                                                                                                                   aCCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTAc                                                                                <  4:73721/141‑1 (MQ=255)                                                                                                                                                                   aCCCCACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGata                     >  1:644040/1‑200 (MQ=255)                                                                                                                                                                    ccccACGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTAcc                                                                               >  4:142240/1‑141 (MQ=255)                                                                                                                                                                         cGATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGCTCTCCATCGCGCCAGATATAAAAc               <  1:753152/200‑1 (MQ=255)                                                                                                                                                                          gATCGGCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCaaa                                                                         >  4:506032/1‑141 (MQ=255)                                                                                                                                                                               gCCCAGAACTTACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCgg         >  1:577833/1‑200 (MQ=255)                                                                                                                                                                               gCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTg                                                                    >  4:50442/1‑141 (MQ=255)                                                                                                                                                                               gCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTg                                                                    >  4:538031/1‑141 (MQ=255)                                                                                                                                                                               gCCCAGAACTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCgg         >  1:683612/1‑200 (MQ=255)                                                                                                                                                                                      aCTCACAGAC‑TTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGc  <  1:472422/200‑1 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                               GTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCACGATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGCTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGC  >  NC_000913/4463967‑4464348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.