| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,978,440 | (G)6→7 | intergenic (‑243/+78) | flhD ← / ← insB1 | flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B |
| Read alignment evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | change | freq | score | reads | annotation | genes | product | ||
| * | NC_000913 | 1,978,434 | 1 | .→G | 100.0% | 149.8 | 42 | intergenic (‑237/+84) | flhD/insB1 | flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B |
| Reads supporting (aligned to +/- strand): new base (17/23): ref base (2/0): total (19/23) | ||||||||||
TTCACCGCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGA‑GG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTG > NC_000913/1978237‑1978622 | ttCACCGCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGA‑gg < 1:523523/200‑1 (MQ=255) gCACCCCGTGATGTCGCCGGGAAGCCCCTGTAAAAAATAATTAGCATTAGAATAGTTGCGATCAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGA‑gg‑ggg‑ggc < 1:617845/200‑3 (MQ=255) ccccGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGA‑gg‑ggg‑ggctgc > 1:553056/1‑195 (MQ=255) aaaTAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATcacac < 1:662910/200‑1 (MQ=255) atTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAac < 1:178040/200‑1 (MQ=255) tAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAacac < 1:160997/200‑1 (MQ=255) tAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAacac < 1:444764/200‑1 (MQ=255) gCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTc > 1:171008/1‑200 (MQ=255) ttAGAATAGTTGCGATAAGCGGCAATAAGCAGAACCACCGTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGA‑gg‑gg < 2:304956/150‑1 (MQ=255) tAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGA‑gg‑ggg‑ggctg < 2:747800/150‑5 (MQ=255) cTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCaataa < 2:416094/150‑1 (MQ=255) gCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATg > 2:190797/1‑150 (MQ=255) gCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGA‑GG‑GGGTGCTGCGAttt > 4:558486/1‑141 (MQ=255) aaGCAGAACTACCTTCTTGGTTGAATATGTCCTTACAAATAGACATGGGGCTTTACGCTTATCTAAGATTATTCCTAAATCGACGTAACTGTCCTCGTCACTACACGCACATACAACGGA‑GGTGGG‑GCTGAGATTTTCAATAACCCGTGa > 2:506820/1‑150 (MQ=255) aaGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACaaa > 1:378234/1‑165 (MQ=255) cctccTTTTTTGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATc < 2:546159/147‑1 (MQ=255) accTTTTTGGTTTAATATGTCCTTACAAATAGAAATGTGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGTAGATtac > 2:221358/1‑150 (MQ=255) tttGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACaaa < 2:378193/150‑1 (MQ=255) tGGTTTAATATGTCCTTACAAATAGAAATTGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATc < 4:283371/141‑1 (MQ=255) ttAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTTTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGAGATTTTCAATAATGCGTGATGCAGATCACACAAAACACGCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttc < 1:397215‑M1/200‑33 (MQ=255) aaTATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACaa > 4:556575/1‑141 (MQ=255) cTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATcacaca > 3:611953/1‑132 (MQ=255) aCAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACAT < 2:801842‑M1/150‑1 (MQ=255) gAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc > 1:317424‑M1/1‑148 (MQ=255) tGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataag < 2:240030‑M1/150‑7 (MQ=255) gTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTGCACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgc > 1:748625‑M1/1‑141 (MQ=255) acacTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataag < 4:471323‑M1/141‑7 (MQ=255) tATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGc > 4:467354/1‑94 (MQ=255) tATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGc < 3:467333/94‑1 (MQ=255) aGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtc < 3:178214‑M1/132‑9 (MQ=255) aaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttt < 3:417227‑M1/132‑21 (MQ=255) cAACTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAAATAAATGtataagtcatacttttgttttgggtgta > 3:170999‑M1/1‑104 (MQ=255) aCTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt > 4:372891‑M1/1‑102 (MQ=255) aCTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttc < 2:20510‑M1/134‑33 (MQ=255) aCTGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttc > 1:20511‑M1/1‑102 (MQ=255) tGTACTCGTCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttc < 3:558454‑M1/132‑33 (MQ=255) cTCGTCACTACGCGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatc < 3:434100‑M1/132‑37 (MQ=255) tcatcacTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTGTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgc < 1:478188‑M1/197‑106 (MQ=255) tCACTACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca > 1:314769‑M1/1‑92 (MQ=255) tACACGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctag < 2:552998‑M1/150‑63 (MQ=255) cacGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATG > 1:257694‑M1/1‑86 (MQ=255) cacGCACATACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATG < 2:257674‑M1/86‑1 (MQ=255) caTACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataact > 1:272335‑M1/1‑80 (MQ=255) aTACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttc > 4:59010‑M1/1‑79 (MQ=255) aTACAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttc < 3:59008‑M1/111‑33 (MQ=255) cAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTAc < 2:117345/64‑1 (MQ=255) cAACGGAGGG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTAc > 1:117353/1‑64 (MQ=255) | TTCACCGCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGA‑GG‑GGG‑GCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTG > NC_000913/1978237‑1978622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |