Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2510T.47.6% inf / inf 1420intergenic (+215/‑327)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (384/290);  new base . (464/149);  total (859/561)
Fisher's exact test for biased strand distribution p-value = 1.31e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-05

Only 100 of 1753 total aligned reads displayed.

TAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACT  >  CP016816/165124‑165378
                                                                                                                                   |                                                                                                                               
 aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑tt                                                                                                                                >  1:3748630/1‑128 (MQ=255)
 aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑tt                                                                                                                                >  1:3884529/1‑128 (MQ=255)
  atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑ttt                                                                                                                               >  2:206924/1‑128 (MQ=255)
     tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGGAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑tttttt                                                                                                                            >  2:3717570/1‑128 (MQ=255)
       tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑tttttttt                                                                                                                          >  2:614484/1‑128 (MQ=255)
         gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGA‑t‑tttttttttt                                                                                                                        >  2:1212368/1‑128 (MQ=255)
         gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGA‑t‑tttttttttt                                                                                                                        >  2:1410288/1‑128 (MQ=255)
          tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑ttttttttttt                                                                                                                       >  2:717519/1‑128 (MQ=255)
            tCATTAATTTATCGCACTTGTATGAAGTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑ttatttttttttt                                                                                                                     >  2:505446/1‑128 (MQ=255)
              aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑ttttttttttttttt                                                                                                                   >  2:2980187/1‑128 (MQ=255)
                    ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑ttttttttttttttttatttt                                                                                                             >  1:1805620/1‑123 (MQ=255)
                      aTCTCACATGTATGAATTCGTAAGAAACTAAAAATATTATAGTTTTATTTATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTtataatgg                                                                                                           <  1:2965622/128‑5 (MQ=255)
                      aTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑tttttttttttttttataatgga                                                                                                           >  1:2231254/1‑120 (MQ=255)
                        cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑tttttttttttttttttaaattaaa                                                                                                         >  2:1273380/1‑120 (MQ=255)
                        cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑t‑tttttttttttttatttattggaat                                                                                                         >  1:2549195/1‑120 (MQ=255)
                           tttttttttaaTTATTTAGATACAAAAAATATTATTTTTTTATTTATTTAATTTATATTAAAATTTTTAAATAATTTATTTTAAAATTTAAAAAA‑‑‑‑‑‑‑‑‑TTCATTTTTTTTTTTTTTATAATGGAAATATaa                                                                                                 <  1:756256/119‑1 (MQ=255)
                              tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑TTTTTTTTTTTTTTTTTTATAAttaaataata                                                                                                  >  2:2151848/1‑119 (MQ=255)
                                tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTTTATAATGTAAATATaa                                                                                                 >  1:1419590/1‑128 (MQ=255)
                                 aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTTTTTaaatgtaaatttta                                                                                                >  1:1052627/1‑116 (MQ=255)
                                  tGAATTAGAAACAAACTAAAAATTTTATATTTTTTTATATTTTTTTTATTTTTAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAA‑‑TATC‑‑‑ATTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  2:4138934/128‑1 (MQ=255)
                                    aaTTATTAACAAACTAAAATATTTAACTTTTTATATATTTTTTTTTTAATCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                                                          <  1:1593461/128‑1 (MQ=255)
                                     aTTACTAATAAAAAAAAAATATTTACTTTTTATTTATTTTTTTTATTTTCAAATTTTTAAAAAATAAATTTGAACATTTAAAAAA‑‑AA‑‑‑T‑CTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                                                          <  2:3804830/128‑1 (MQ=255)
                                      ttattaATAAAATAAAAATATTATATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAAATAATTTTAACTTTTAAAAAA‑‑AAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                                                        <  2:4065442/128‑1 (MQ=255)
                                      ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATTAAAATTTTAgt                                                                                         >  1:868441/1‑127 (MQ=255)
                                       ttttaataaAATAAAACTTTTATATTTTTATATATTTTTTGTATATTAAAATTTTTAAAAAATTAAATTTAAATTTTAAAAAA‑‑T‑‑‑‑T‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                                                       <  2:4509332/125‑1 (MQ=255)
                                       tatttattaaataaaaataTTTTGTTTTTATATATTTTTTTTATTTTGAAATTTTTAAAAAATTTATTTTTACTTTTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                                                        <  2:3580012/117‑1 (MQ=255)
                                           aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTAtttt                                                                                   >  1:4147132/1‑128 (MQ=255)
                                             gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTATATAATTGAAATAAAAATTTTATGTAttt                                                                                    >  2:2536486/1‑128 (MQ=255)
                                             gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATTTAAAAAAAAATTTTATTTAtttt                                                                                   >  1:2735577/1‑128 (MQ=255)
                                             gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTGAATTATAAATTTTATTTAttttt                                                                                  >  2:1901847/1‑127 (MQ=255)
                                              aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TATC‑‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTattttttttt                                                                                  >  2:3208048/1‑119 (MQ=255)
                                                  taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAATTTTTAGTTATTTttttgtat                                                                            >  2:1619056/1‑126 (MQ=255)
                                                   aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTT‑‑‑TAATGTAATTATAAATTTTAGTTATttttttttaaa                                                                           >  1:636149/1‑123 (MQ=255)
                                                     aactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTAAATTTAAAATATAAATTTTATTTATtttttttaataa                                                                          >  1:4350676/1‑122 (MQ=255)
                                                      actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATaat                                                                         >  2:3166117/1‑127 (MQ=255)
                                                       ttaatttttttttATATATTTTTTTTATTTGCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAA‑‑TTTC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                                                          <  2:3532265/120‑1 (MQ=255)
                                                       atattttatttTTAGATATTTTTTTTATTTTTAAATTTTTAAAAAAATAATTTTAAATTTTTAAAAA‑‑TA‑‑‑‑‑TCTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                                                       <  2:3306776/127‑1 (MQ=255)
                                                        tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTT‑‑TAATGAAAATTTAAATTTTAGtttttttttttgtatatta                                                                       >  1:3665687/1‑119 (MQ=255)
                                                           tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTATTTTTTTTTTTTTATAAAGTAAATATAAATTTTATTTATTTTTTTTTATAATTAtaa                                                                    >  1:2619824/1‑126 (MQ=255)
                                                              cTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTATTTTTTTTTAAAAAAGAAAATATAAATATTACTTATTTTGTTTAATAAGAATCTaaa                                                                 >  2:934688/1‑128 (MQ=255)
                                                              cTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaa                                                                >  1:125944/1‑128 (MQ=255)
                                                               aaaaatttttttttttttATTTTTAAATTTTTAAAAATTTATTTTTTAATTTTAAAAAA‑‑TA‑‑‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaa                                                               <  2:3971906/118‑1 (MQ=255)
                                                                ttttATATATTTTTTGTATTTTCAAATTTTTAAAAAATTTATTTTAAATTGTTAAAAA‑‑T‑‑T‑T‑CTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTGTTGTCTAAGTATCTaaaaaa                                                              <  2:829173/128‑1 (MQ=255)
                                                                 tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATATTGAAAATAAAAATTTTAGGTATTTTTTTGTATAAGttaaaaaaaaa                                                              >  1:2346440/1‑118 (MQ=255)
                                                                  ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTT‑‑‑‑ATTGAAATATAAATTTTTTGTTTTTTTTTTTATAAATATATTAAAAAATa                                                           >  1:1673276/1‑128 (MQ=255)
                                                                   tAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑‑‑‑TTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                                                        >  2:1035588/1‑128 (MQ=255)
                                                                    ttttatTTTTTTTATATTAAAATTTTTAAAAAAATAATTTTAACTTTTAAAAAA‑‑TA‑‑‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                                                          <  1:1000910/125‑1 (MQ=255)
                                                                    aGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTGTTTTCTAATTATCTAAAAAAATata                                                         >  2:3655262/1‑126 (MQ=255)
                                                                       ttttttttttATATGAAAATTTTTAAAAAATTAAATTTTAATTTTAAAAAA‑‑TTTC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                                                         <  1:4073591/126‑1 (MQ=255)
                                                                            tttttatattataaTTTTTAAAAAATTAATTTTAATTTGTAAAAAA‑‑AATC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                                                    <  1:1159144/116‑1 (MQ=255)
                                                                            ttgttattTGCATTTTTTAAAA‑AATTAATTTTTACTTTTAAAAAA‑‑TA‑‑‑T‑GTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                                                  <  1:4229760/124‑1 (MQ=255)
                                                                             ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTT‑‑TAATGTAAATTTAAATTTTAGGTATTTTGTTGTAAAATTATATAAAAAAATATAAAGTaaa                                                  >  1:4429037/1‑128 (MQ=255)
                                                                               ttataTTCAAAATTTTAAAAAATTAATTTTAAATTGTAAAAAA‑‑TAGC‑‑‑‑ATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa                                                <  2:4274964/127‑1 (MQ=255)
                                                                               ttaaatataaaTTTTTAAAAAAATAATTTGAAATATTAAAAAA‑‑AATC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                                                  <  1:3974604/120‑1 (MQ=255)
                                                                               gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATTTTAATTTTAGGTATTTTTTTTTCTAAGTATCTAAAAAAATAGaatgaataaa                                                >  1:379760/1‑120 (MQ=255)
                                                                                 tattttaaaTTTTTTAAAAATTAATTTTAAATTTTAAAAAA‑‑TA‑‑‑T‑CTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                                              <  2:458804/122‑1 (MQ=255)
                                                                                 atatGCAAATTTTTAAAAAATTTATTTGAAATTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata                                             <  1:3039389/128‑1 (MQ=255)
                                                                                 atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATTTATTTTTTTGTATAAATTAATAAAAAAAAATAAAATAAAAAat                                              >  1:2488678/1‑128 (MQ=255)
                                                                                  ttttataaTTTTTAAAAAATTAATTTTTAATTGTTAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata                                             <  2:3863745/122‑1 (MQ=255)
                                                                                  tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGCTT‑TTTTTTTTTTTTTTTTTTATAATTTAAATTAAATTTTTTGGTATTTTTTATTAAATTTATTTAAAAAAATATAAAGGaaaaa                                                >  1:951777/1‑128 (MQ=255)
                                                                                    tcaaatttttTAAAAAATAAATTTTAA‑TTTTAAAAAA‑‑TA‑‑‑T‑CTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTg                                          <  1:518564/122‑1 (MQ=255)
                                                                                    tGCAAATTTTTAAAAAATTAATTTGAACTTTTAAATAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTg                                          <  1:3606923/128‑1 (MQ=255)
                                                                                      cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTTT‑TAATGGAAAAATAAATTTTAGTTATTTTTTTTTAAAATAATATAAAAAATAAAAAAGTAAAAattattt                                          >  1:2854819/1‑127 (MQ=255)
                                                                                      cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAATTTTTAGTTTTTTTTTTTTATAATTTTATAAAAAAATAAAAATAAAAAAATAtttt                                         >  2:1812032/1‑126 (MQ=255)
                                                                                         aTTTTTAAAAAATTAATTTGAACTCGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTT‑‑TAATGGAAATAAAATTTTTAGGTATTTTGTTGTTTATTTTACAAAAAAAAAACAAAGTTAAAAATTTTGTTaa                                      >  1:2415129/1‑128 (MQ=255)
                                                                                          tttttaataaattaattTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTTTATATTGGAAATATTATTTTTAGGTATTTTTTTTTATAATTTTATAAAAAAATAGAATTGAAAATATTTTTTTa                                       >  2:1990212/10‑128 (MQ=255)
                                                                                             tttaaaaattaattTTAAATTTTAAAAAATTTTTT‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa                                      <  2:4200154/122‑1 (MQ=255)
                                                                                             ttaaaaaaTTTATTTTAATTTTTTAAAAA‑‑TAG‑‑‑‑ATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt                                  <  2:1551745/126‑1 (MQ=255)
                                                                                             ttaaaaaaTTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTAAAATGAAAATTTAAATTTTAGTTTTTTTTTTGTATAATTATATATAAAAATATAATGGTAATAAAATTTTTaaatta                                  >  1:2028557/3‑125 (MQ=255)
                                                                                              taaaaaaTTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGAAATTATAAATTTTAGGTATTTTTTTTTATAATTATATAAAAAAATTAAAATGAAAAATTTTTTTTAAATGTa                                 >  2:3362074/2‑128 (MQ=255)
                                                                                                 aaaattaattTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTTTCTAAGTATCTAAAAAAATATAAAGTAAAAAATATTTTTAAAAGTAATa                              >  2:1706141/3‑128 (MQ=255)
                                                                                                  aaattaattTTTAATTTTAAAAAA‑‑TA‑T‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATa                              <  1:1353500/127‑1 (MQ=255)
                                                                                                  aaattaattTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTTTATAATGAAAATAAAAATTTTTGTTTTTTTTTTTAATATTATTATAAAAAAATATAATATAAAAAATATTTTTAAAATTAAt                               >  2:3440034/2‑128 (MQ=255)
                                                                                                  aaattaattTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTGTTTTATAATTTTTTAAAAAAATATAAATTAAAAAATATTTTTAAAAGTaaaaat                            >  2:2882661/2‑124 (MQ=255)
                                                                                                   aattaattTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTTTATATTGGAAATATAATTTTTAGTTATTTTTTTTTATAATTATATAAAAAAATATAAAGGAAAAAATATTTTTaatattatta                              >  2:1478696/1‑120 (MQ=255)
                                                                                                   aattaattTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGTTATTTTGTTGTGTAATTAAATAAAAAAATATAAAGTAAAAATTATTTTTAAAAGTATTata                            >  2:311314/1‑126 (MQ=255)
                                                                                                   aattaattTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTAAAATTAAAATATAAATTTTAGTTTATTTGTTTTATAATTAAAAAAAAAAAAAAAAAATAAAAAATATTTTTaaaataaaaaaa                            >  1:1900381/1‑120 (MQ=255)
                                                                                                    attaattTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTAATTTTGTTTTATAAGTTTCTAAAAAAATATAAAGGAAAAAATATTTTTAAATTtaataataa                          >  2:561797/1‑128 (MQ=255)
                                                                                                     ttaattTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTTTATAATTATATAAAAAAATAGAATGTAAAAAATATTTTTAAAATTAATAATaaa                         >  2:2680190/1‑128 (MQ=255)
                                                                                                      taattTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTTTTTAATGTAAATAAAAATTTTTGTTTTTTTTTTTTATAATTTAATAAAAAAAAATAATTTAAAAAATATTTTTAAAAataataaaa                           >  2:2449111/1‑125 (MQ=255)
                                                                                                      taattTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTATAAGATTAAAAAAAAATATAAAGTAAAAAAATTTGTTAAAAGTATTAATaaaa                        >  1:1742350/1‑128 (MQ=255)
                                                                                                       aattTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTGTTGTATAATTATATAAAAAAATAGAAAGTAAAAAATATTGTTAAAATTAATAATAAAatt                      >  2:2617463/1‑128 (MQ=255)
                                                                                                        attTTAACTTGTAAAAAA‑‑TA‑‑‑‑‑TGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAatt                      <  1:4295794/128‑1 (MQ=255)
                                                                                                            gAACTTGT‑AAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTTTGTATTTTGTTGTATATGTTTATTAAAAAATAGAAAGGATAATATATTGTTAATAATTATAATAAAattatta                  >  1:3149842/1‑127 (MQ=255)
                                                                                                             aaCTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTTGTATTTTGTTGTATAAGTATATAAAAAAATATAAAGGAAAAAATATTTTTAAAAGTAATACTTAAattaatta                 >  2:2219318/1‑128 (MQ=255)
                                                                                                              aCTTTTAAAAAA‑‑TATC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTAttt                  <  1:3523410/128‑1 (MQ=255)
                                                                                                               cTTGTAAAAAA‑‑TAGC‑‑‑‑‑‑TTTTTTTTTTTTTAAAAATTTTAAATAAAAATTTTAGATATATTGTATAATAAATAAAAAAAAAAATATAAATGAAAAAATTTTTTTAAAAAATATAAAAAAattattaatta              >  1:3299273/1‑123 (MQ=255)
                                                                                                                ttGTAAAAAA‑‑TAGC‑‑‑TTTTTTTTTTTTTTTTTTA‑AATGGAAATATAAATTTTTGGTTTTTTTTTTTAAAATTAAAAAAAAAAATATAAAGGAAAAAATTTTTTTAAATTTAAAAAATAATTTATTTAAt               >  1:4622043/1‑128 (MQ=255)
                                                                                                                 tGTAAAAAA‑‑TAGC‑‑‑TTTTTTTTTTTTTTTTTTAAAATGGAAATATAAATTTTAGGTATTTTTTTTTCTAATTATATAAAAAATTATAATATAAAAAATATTTTTAAAATTAATACTAATATTATTTAAt               >  2:275906/1‑128 (MQ=255)
                                                                                                                  gTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTTGTTATTTTTTTGTATAATTAAATAAAAAAATAAAAAGTAAAAAATATTTTTAAAATTAATAAAAAAATTATTTAAttt             >  1:1510152/1‑126 (MQ=255)
                                                                                                                   tAAAAAA‑‑TAGC‑‑‑TTTTTTTTTTTTTTTTTAATAATGTAAATTAAATTTTTATGTATTTTTTTTTTTAATTTAAAAAAAAAAAATAAAGAAATAAAATTTGTAAAAATTAATAATAAAATTATTAAAtta             >  2:2310311/1‑126 (MQ=255)
                                                                                                                   tAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATATTTGAAATAAAAATTTTAGTTTTTTTGTTGAAAAAGTATATAAAAAAATAAAAAATAAAAAAAATTTTTAAAATTTATAAAAAAATTATTTAAtttt            >  1:3223366/1‑128 (MQ=255)
                                                                                                                    aaaaaa‑‑aaGC‑TATTTTTTTTTTTTTTTTTTTAAATGTAAATAAAAATTTTTGTTATTTTTTTTTATAATTATAAAAAAATAAATAAAGTAAAAAAATTTTTTAAATGTAAAAATAAAATTATTAAAtt              >  1:2163466/1‑127 (MQ=255)
                                                                                                                      aaaa‑‑TAGC‑T‑TTTTTTTTTTTTTTTTT‑‑TAATGTAAATATAATTTTTAGGTATTTTGTTGTATATGTATAAAAAAAAATAAAAAGTAAAAAATATTTTTAAAATTAATAATAAAATTTtttaattttaat         >  2:1345359/1‑128 (MQ=255)
                                                                                                                       tttaaaaatat‑ttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGt             <  2:4350676/118‑1 (MQ=255)
                                                                                                                       aaa‑‑TAGC‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATTTAAATTTTTGGTTTTTTGTTGGATAATTATCTAAAAAAATAGAAAGGAAAAAATATTTTTAAATGTAATAATAAAATTTtttaattttaat         >  2:2373055/1‑128 (MQ=255)
                                                                                                                          tattcttt‑ttttttttttttttttttATAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaa          <  1:4520825/121‑1 (MQ=255)
                                                                                                                            aaattt‑ttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatt        <  2:4444934/123‑1 (MQ=255)
                                                                                                                              aaatattttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta       <  1:3391675/123‑1 (MQ=255)
                                                                                                                                  cttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAATATTTTTAAAATTAATAAAAAAATTATTTAATTTAAATAAAAAc   >  2:1125968/2‑128 (MQ=255)
                                                                                                                                   |                                                                                                                               
TAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑T‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACT  >  CP016816/165124‑165378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑