Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA98.7% 5251.9 / 37.3 1679intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1025/624);  minor base . (3/18);  total (1031/648)
Fisher's exact test for biased strand distribution p-value = 1.02e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1723 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAA  >  CP016816/165097‑165348
                                                                                                                              |                                                                                                                              
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTaaaaaa                                                                                                                              >  2:1078224/1‑128 (MQ=255)
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTaaaaaa                                                                                                                              >  2:3924774/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAaat                                                                                                                             >  1:495101/1‑128 (MQ=255)
   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAaatta                                                                                                                           <  2:4589002/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTg                                                                                                                      >  1:3141826/1‑128 (MQ=255)
         tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                     <  1:3190885/128‑1 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTGTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                               >  1:160314/1‑128 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                             <  2:3653664/128‑1 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                            >  1:2832023/1‑128 (MQ=255)
                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                           >  2:3539809/1‑128 (MQ=255)
                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTAATATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                           <  1:260735/128‑1 (MQ=255)
                    tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                          <  1:3676184/128‑1 (MQ=255)
                    tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                          >  2:1759731/1‑128 (MQ=255)
                    tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                          >  2:3391675/1‑128 (MQ=255)
                      gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTCTAAAAAAt                                                                                                        >  2:3273854/1‑128 (MQ=255)
                        tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATat                                                                                                      >  2:2616594/1‑127 (MQ=255)
                        tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAACAAACAAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                      <  1:1461601/128‑1 (MQ=255)
                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                                   >  2:4448057/1‑128 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                  >  1:3748630/1‑128 (MQ=255)
                             ataaTTAGTTTCATTAATTTATCGCACTTGTATGATTTAGTAAGAAACTAAAACTATTATCTTAATAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATATGAACTTTTAAATAATAGCtttt                                                                                                 >  1:4580508/1‑128 (MQ=255)
                               atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttt                                                                                               >  2:1959544/1‑128 (MQ=255)
                                tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttt                                                                                              >  1:3798979/1‑128 (MQ=255)
                                 ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttt                                                                                             >  2:1162816/1‑128 (MQ=255)
                                  tattttCATTAATTTATGGCATTTGTTTGAATTTATAATTAACTAAAACTATTATTTTTTTAGTTATTTTTTTTA‑TTTGAAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATAGCttttttttt                                                                                            <  1:606322/125‑1 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                            >  1:723539/1‑128 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttat                                                                                            >  2:3184946/1‑126 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATAATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAATATAGCtttttttta                                                                                            >  1:4114739/1‑127 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                          >  1:2196618/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                          >  2:2350317/1‑128 (MQ=255)
                                      ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACCATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttt                                                                                        >  2:4073624/1‑128 (MQ=255)
                                            aaTTTATTGCAATTTTATTTATTATTAATAAAAAAAAACTATTATCTTTTTATATATTTTTTTTT‑TATTCAAATTTTTAAAAAATTAAATTGAACTTTTAAAAAAAAGCattttttttttttttttat                                                                                  <  2:4019286/128‑3 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTATTTTTTTTtataa                                                                                >  2:4357912/1‑127 (MQ=255)
                                               ttttagCACTTGTATGAAATATTAAGAAACTAAAACTTTTATCTTTTTATATATTTTTTTTT‑TATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttttttttttttttttataatg                                                                               <  2:1237429/123‑7 (MQ=255)
                                                tATCACACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttaaaat                                                                              >  2:1353500/1‑123 (MQ=255)
                                                 aTCTCACATGTTTTAATTAGTAACAAACAAAAAAAATTATATTTTTTTATATTTTTTGTT‑TATTCAAATTTTTAAAAAAATAATTTTAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTtataatgg                                                                             <  1:201025/128‑5 (MQ=255)
                                                   cgctattttattaattattaatAAAAAAAAAATATTTATTTTTTAGATTTTTTTTTTT‑AATTCAAATTTTTAAAAAAATTATTTTTACTTTTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGa                                                                          <  1:4385000/120‑1 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAATATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTttatttatttaaa                                                                           >  1:1385672/1‑123 (MQ=255)
                                                      aCTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGTaatt                                                                       >  1:1297178/1‑126 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAata                                                                      >  2:918761/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTGAAatat                                                                     >  1:3857494/1‑128 (MQ=255)
                                                         tGTATGAAATATTAATTAACTAAAAATATTATTTTTTTAGATATTTTTTTTA‑TATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                   <  2:1418509/128‑1 (MQ=255)
                                                          ttattaattattaatAAAATAAAAATTTTATTTTTTTATTTATTTTTTTTA‑TATGTAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAA‑‑‑‑TTGGTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                <  2:1854483/127‑1 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGTAAATATaaa                                                                  >  2:2280530/1‑128 (MQ=255)
                                                                  tatttattaaataaaaataTTTTGTTTTTATATATTTTTTTTA‑TTTTGAAATTTTTAAAAAATTTATTTTTACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                          <  2:3580012/117‑1 (MQ=255)
                                                                  tattaataaACTAAAAATATTATATTTTTATATATTTTTTTTA‑TATTCAAATTTTTAAAAAATTTATTTTAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                         <  2:4279927/125‑1 (MQ=255)
                                                                   ttttataaaataaaaataTTATTTTTTTTTTTATTTTTTTTT‑TTTTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                          <  1:1162816/122‑1 (MQ=255)
                                                                   attaataaAATAAAAATATTATTTTTTTATTTATTTTTTTTT‑TTTTCAAAATTTTTAAAAATTAATTTTTAAATTTAAAAAATATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                          <  1:3916440/126‑1 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTaat                                                       >  1:929225/1‑126 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGGAATTATAAATTTTAGTTAtt                                                       >  1:3306776/1‑128 (MQ=255)
                                                                     tAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATTAAAATATAATTTTTAGTTAttt                                                      >  2:2697940/1‑128 (MQ=255)
                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTAAGTTAtt                                                       >  2:618465/1‑128 (MQ=255)
                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAG‑‑ATTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTAATTAttttt                                                    >  2:3323306/1‑127 (MQ=255)
                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATAGAAATTTAATTTTTAGTTAtttttt                                                   >  2:1576333/1‑126 (MQ=255)
                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTAATTTAtttttt                                                   >  2:2119008/1‑126 (MQ=255)
                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTAGTTATTttgt                                                   >  2:4144217/1‑128 (MQ=255)
                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATtttttt                                                  >  2:1298499/1‑128 (MQ=255)
                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATttttttt                                                 >  1:1053593/1‑127 (MQ=255)
                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGGAAATATAAATTTTAGGAATTTTGTTGTCt                                              >  1:2113264/1‑128 (MQ=255)
                                                                               aaatatatatTTTTTTAATTTTTTTTTTTATTTTGAAATTTTTAAAAAAATTATTTTTACTTTTTAAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                <  1:818765/121‑1 (MQ=255)
                                                                               aaaaaattttattttttTATATTTTTTTTT‑TTTTTAAATTTTTTAAAAATTAAATTTAAATTGTAAAAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                             <  2:2955613/117‑1 (MQ=255)
                                                                               aaaaaattatCTTTTTATATATTTTTTTTT‑TATTAAAATTTTTAAAAAAATAATTTTAAATTTTAAAAAAAA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                             <  1:4023336/123‑1 (MQ=255)
                                                                                 actaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAT‑‑TGTAAATATAAATTTTAGTTATTTttttgtaaaat                                           >  1:2532406/1‑123 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTA‑TAAGCAAATCTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGTAAATATAAAATTTATTTATTTTGTTTTATAATTa                                         >  1:1529141/1‑128 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTTTTTATTTTTTTTTATAATTa                                         >  2:2428127/1‑128 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTCTAAGTAta                                       >  2:2830190/1‑127 (MQ=255)
                                                                                    attatATTTTAATATAATTTTTTTA‑TATTTTAATTTTTAAAAAATTAATTTTAAATTTT‑‑TAAATAATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                        <  1:343888/128‑1 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTAGTTATTTTGTTGAATAATTATc                                       >  2:565633/1‑128 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATTAAAATTTTAGGTATTTTGTTGTATAAGTATATaa                                    >  2:3699729/1‑128 (MQ=255)
                                                                                          tttttATATATTTTTTTTA‑TATTCATATTTTTAAAAAATTAATTTGAAATTTTAAAAAATA‑‑‑TAATTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTaaaaa                                 <  2:2196493/128‑1 (MQ=255)
                                                                                          tttttAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTATAATTATCTaaaa                                  >  2:4515425/1‑128 (MQ=255)
                                                                                             tttttttatttttttt‑TATTTAAATTTTTTAAAAATTTATTTTTAATTTTTAAAAAT‑TTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaa                                <  2:1499448/120‑1 (MQ=255)
                                                                                             ttatttatTTTTTGTA‑TATTCAAATTTTTAAAAAAATAATTTGAAATTTTAAAAAAAAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAAt                              <  1:3615289/123‑1 (MQ=255)
                                                                                             ttAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑‑‑ATTGAAATATAAATTTTTTGTTTTTTTTTTTATAAATATATTAAAAAATa                             >  1:1673276/1‑128 (MQ=255)
                                                                                             tatattatTTTTTTTA‑‑ATTTAAATTTTTATAAAATTAATTTGTAATTTTTAAAAA‑A‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                             <  1:449546/123‑1 (MQ=255)
                                                                                              tAGATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTATTTATTTTTTTTTATAATTTTCaaaaaaaa                               >  1:2377308/1‑128 (MQ=255)
                                                                                               ttttatTTTTTTTA‑TATTAAAATTTTTAAAAAAATAATTTTAACTTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                            <  1:1000910/125‑1 (MQ=255)
                                                                                               atatatTTTTTTTT‑TTTTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                             <  2:3852405/126‑1 (MQ=255)
                                                                                                gATATTTTTTGTA‑TATGTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                            <  2:2523084/128‑1 (MQ=255)
                                                                                                gATATTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATGGAAAAATAAATTTAAGGTATTTTGTTTTTTAAGTATATAAAAAAATa                             >  2:817609/1‑128 (MQ=255)
                                                                                                 tttttttttttA‑TATTAAAATTTTTAAAAAATTAATTTGTACTTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                          <  2:100534/125‑1 (MQ=255)
                                                                                                 atatTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTGAATAAAAAATTTAAGCTATTTTTTTATGAAACTAAATaaaaaaaaat                            >  1:2458528/1‑125 (MQ=255)
                                                                                                   tttttttTGA‑TAATCAAATTTTTAAAAAAATAATTTTAAATTTTAAAAAATA‑‑‑‑‑TCGTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                      <  1:1750478/127‑1 (MQ=255)
                                                                                                   atTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTATAATTTTATAAAAAAAAATaaa                         >  2:712915/1‑128 (MQ=255)
                                                                                                   atTTTTTGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAG‑ATTTTTTTTTTTTTTTTTTATAATTTAAATATAAATATTATTTATTTTTTTTTATAATTATAaaaaaaaaaaaaa                          >  1:2697989/1‑123 (MQ=255)
                                                                                                       aattta‑taTTAAAATTTTATAAAAATATATTTTAAATTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                     <  1:2931790/124‑1 (MQ=255)
                                                                                                        ttGTA‑TATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTTGTTTTTTTTTTGTATAATTATATAAAAAAATAAAAATGaa                     >  2:3793462/1‑128 (MQ=255)
                                                                                                            a‑tatGCAAATTTTTAAAAAATTTATTTGAAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata               <  1:3039389/128‑1 (MQ=255)
                                                                                                               atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCATTTTTTTTTTTTTTTTT‑‑TAATTGAAATAAAAAATTTAATTTTTTTTTTTTATAAGTTTGAAAAAAAAAATATATGAAAAtataa              >  1:4309880/1‑127 (MQ=255)
                                                                                                                tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATTTAAATTTTAGGTTTTTTGTTGTATAAGTTTCTAAAAAAATATAAATGAAAAAatat              >  2:1659933/1‑128 (MQ=255)
                                                                                                                tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTTTTTTTTTTTATAATTTTATAAAAAAATATAAATTAAAAAATAtt             >  2:4191642/1‑128 (MQ=255)
                                                                                                                  cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGAAATTTTAAATTTTAGGTATTTTTTTTTCTAAGTTTAAAAAAAATTTTAAGGGAAAAAAtttttt           >  1:2298637/1‑126 (MQ=255)
                                                                                                                  cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATTTAAATTTTAGTTTTTTTTTTGTATAATTATCAAAAAAAATAAAAATGAAAAAATAttt            >  2:3126351/1‑127 (MQ=255)
                                                                                                                  cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTTTATTTTAATAAAAAAATTTAAATAAAAAAATAtttt           >  2:492493/1‑126 (MQ=255)
                                                                                                                  cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGAAAAAAAAAATTTTAGTTATTTTGTTATATATTTATAAAAAAAAATATAATTCAAAAAATAtttt           >  2:3043943/1‑126 (MQ=255)
                                                                                                                   aaaTTTTTTAAAAATTAATTTTTATTTTTAAAAAAT‑TTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGt           <  1:3539809/128‑1 (MQ=255)
                                                                                                                    aaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAATTTTATAAAAAAATATAAATTAAAAAAtttttta          >  2:2025693/1‑125 (MQ=255)
                                                                                                                     aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTTTATAATTATGTAAAAAAATTTAAAGGAAAAATTATTGTTaa        >  2:345147/1‑128 (MQ=255)
                                                                                                                      ttttAAAA‑AATTAATTTGAACTTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt    <  1:1821087/128‑1 (MQ=255)
                                                                                                                         ttaaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATGGAAATTAAAATTTTTTGTATTTTTTTGTAAAATTATATAAAAAAAAATAAAGGAAAAAATTTTTATaaaa      >  1:4151578/3‑128 (MQ=255)
                                                                                                                           aaaaaaTTAATTTGTACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTaa  <  1:4233159/128‑1 (MQ=255)
                                                                                                                              |                                                                                                                              
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAA  >  CP016816/165097‑165348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑