Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4880T.38.2% 702.1 / inf 629intergenic (+266/‑409)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (190/168);  new base . (40/181);  total (252/377)
Fisher's exact test for biased strand distribution p-value = 1.59e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-06

Only 100 of 689 total aligned reads displayed.

TAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTA  >  CP016816/202367‑202614
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tAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑Ttaaaaaataa                                                                                                                       >  2:5017227/1‑128 (MQ=255)
  aataatAGAGTTAAGTTTAATTAAAATACCATAAACTTAATAAATCTTATAATGTACATCCTCGTGATCTATTTTTATATATTTTTATCTTCACAAAAAAAAAAAAACAAAAACC‑‑Ttaaaaaataaaa                                                                                                                     >  2:3284851/1‑128 (MQ=255)
  aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑Ttaaaaattaaaa                                                                                                                     >  1:5767706/1‑128 (MQ=255)
     aataGTGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaa                                                                                                                  >  2:3715898/1‑128 (MQ=255)
       taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCAACTT‑‑‑AAAAATaaaaaaaaaa                                                                                                               >  1:3258140/1‑128 (MQ=255)
       taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑‑TAAAAAATtaaaaaaaaa                                                                                                               >  2:1996230/1‑128 (MQ=255)
          agTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTTATCTATTTTTATATTTTTTTATCTTCACAAATAAAAAAAAACAATCAAC‑‑TTAAAAATTaaaaaaaaaaaa                                                                                                             >  1:2038790/1‑128 (MQ=255)
           gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATaaaaaaaaaaaaaa                                                                                                           >  1:6012243/1‑128 (MQ=255)
           gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                            >  2:6348724/1‑128 (MQ=255)
            ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑ATAAAAATTaaaaaaaaaaaaaa                                                                                                           >  1:3495635/1‑128 (MQ=255)
            ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTAAAAAATTAAATCATCGTGATCTATTTTTAAATATTATTATCTTCAAAAATAAAAAAAAAAAATAAC‑‑AATAAAAAATaaaaaaaaaaaaaa                                                                                                           >  2:1198286/1‑128 (MQ=255)
             tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                          >  2:5090719/1‑128 (MQ=255)
              aattttaattaaAATAACAAAAACATAATAATTTTTATAATTTATTATCTCGTGATTTTTTTTTTTTTTTTTTTATTTTAACAAATAAAAAAAAAAAATACCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                         <  2:1606541/124‑1 (MQ=255)
              aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAAACACCAT‑‑‑AAAAATaaaaaaaaaaaaaaaaa                                                                                                        >  2:6254655/1‑128 (MQ=255)
              aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAAAAAAAAAAAACAATCCCCTTAaaaaaaaaaaaaaaaaaaaaaa                                                                                                           >  2:1683567/1‑128 (MQ=255)
               aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                        >  2:6598191/1‑128 (MQ=255)
               aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                        >  2:834459/1‑128 (MQ=255)
                ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTATTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                       >  1:45737/1‑128 (MQ=255)
                ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                       >  2:5411575/1‑128 (MQ=255)
                ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                       >  2:758814/1‑128 (MQ=255)
                ggTTAATTAAAATACAAAAAAATTAATAATTCTTATAATTTATATCCTCGTGTTCTTTTTTTTTTTTTTTTTATCATAACAAAAAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                                       <  1:5593111/128‑1 (MQ=255)
                  ttaattaaAATACCATAAACTTAATAATTCTTATAATTTAGATCAACTTTATATAATTATATATAAAAAAATATTAAAAAATAAAAAAAAAAAAT‑‑‑CAAAAAAAAAATaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  2:26926/1‑128 (MQ=255)
                   taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAACAATCAAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  2:6074647/1‑128 (MQ=255)
                   taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATACCC‑‑ATAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  2:5271334/1‑128 (MQ=255)
                   taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCATCGTGATCTAATTTTTTATTTATATATATTCACAAATAAAAAAAAACAAAAAACTTAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                                      >  2:4580865/1‑128 (MQ=255)
                     attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAAACCAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  2:2196970/1‑126 (MQ=255)
                      ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  2:5882116/1‑125 (MQ=255)
                      ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAAAATAAAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  1:2393185/1‑125 (MQ=255)
                      ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTATTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAAAATAAACTTAAAAATaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                   >  2:3846403/1‑127 (MQ=255)
                       taaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑‑TAAAAAATaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                               >  2:6216569/1‑123 (MQ=255)
                       taaAATACCATAAACTTAATAATTATTATAATGTAGATCATCTTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  1:3759719/1‑124 (MQ=255)
                       taaAATACCATAAACTTAAAAAATATTAAAATTTAGAACCTCGTGATCTTTTTTTTTATTTGTTTATATTAACAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTaaa                                                                                               <  1:6316605/128‑1 (MQ=255)
                        aaaataaaataaaatTAAATATTATTATAATTTATAACCTTGTTATCTATTTTTTTATTTTTTTATCTTAACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaactaaaaac                                                                                               <  1:1883182/128‑9 (MQ=255)
                        aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  2:6136106/1‑124 (MQ=255)
                        aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaataa                                                                                               >  2:5789904/1‑123 (MQ=255)
                        aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTAATTTATCTTCACATATAAAAAAAAACAAAACCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                               >  2:2946053/1‑123 (MQ=255)
                        aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTATTTATCTATTTTTTTATTTTTTTATATTCACAAATAAAAAAAAAAAATAACC‑‑TTAAAAAATaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                               >  1:1003267/1‑123 (MQ=255)
                        aaaaTACCAAAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTATTTTATTTGTTTATATTCAAAAATAAAAAAAAACAATACCCTTAAAAATaaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                 >  2:4104281/1‑125 (MQ=255)
                         aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTACACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  1:4957368/1‑128 (MQ=255)
                          aaTACCATAAAATTAATAAATTTTATAATGTATATACTTGTTATATTTTTTTTTATTTTTTTATATTAACAAATAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                           <  2:2341535/128‑1 (MQ=255)
                           attacATAAAATTAAAATATCTTATAATGTATATACTCTTTATCTATTATTTTATTTTTTTATCTTAAAAAATAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                           <  1:3025391/124‑1 (MQ=255)
                            tataaTAAAATTAATAATTATTATAATTTATATCCTTGTTTTTTATTTTTTTTTTTTTTTATATTAAAAAATAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                          <  2:1819405/124‑1 (MQ=255)
                            taacaaaaaaataataaTTCTTATAAATTATTAACTCGTTTTTTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                          <  1:3903601/117‑1 (MQ=255)
                            taaataaaaaTTAATTATTATTATAATTTATATAATTGTTATAATTTTTTTTTTTTTTTTATATTAAAAAAAAAAAAAAAAAAAAACCC‑‑TTTAAAATTAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                           <  1:6436430/122‑1 (MQ=255)
                                 ataaattAATAATTATTATAATGTTTAATCTTGTTATATATTTTTTTATTTTTTAATATTAAAAAATAAAAAAAAAAAAAACCC‑‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                    <  2:5451092/123‑1 (MQ=255)
                                  aaaaataataaTTATTAAAATTTATAACCTCGTGATCTATTTTTTTATTTTTTTAACTTAACAAATAAAAAAAAACAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                    <  1:6551888/123‑1 (MQ=255)
                                  aaaaTTTATAATTATTTTAATTTATTTCATCTTTTTTTATTTTTTTTTTTGTTTTTTTTAAAAAATAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                    <  2:5346603/128‑1 (MQ=255)
                                  aaaaTTAATAATTATTATAATTTAGTACATCGTTATCTATTTTTTTATTTGTTTATATTAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAt                                                                                   <  2:5325973/128‑1 (MQ=255)
                                     tttAAAAATTTTTATAATTTATATCCTAGGTATAATTTTTTTTATTTTTTTATATTCACAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTc                                                                                <  2:4302651/127‑1 (MQ=255)
                                      taaatattTTTTAAAATTTATTTCATCTTTATATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAttt                                                                                 <  1:61431/126‑1 (MQ=255)
                                       taataaTTCTTATAATTTAGATCCTAGGTATCTTTTTTTTTATTTGTTTAACTTCACAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCt                                                                               <  1:5681947/128‑1 (MQ=255)
                                       taaaaaTTTTTAAAATTTATATACTCGTTATTTATTTTTTTATTTTTTTATAATCAAAAAAAAAAAAAAAAAAAACCC‑‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTc                                                                                <  2:3571982/128‑1 (MQ=255)
                                           aatTATTATAATTTATAACATTTTTATATATTTTTTTATTTTTTTATATTAAAAAATAAAAAAAAAAAATACCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaaa                                                                           <  1:144541/128‑1 (MQ=255)
                                            attattATAATTTATATAATCTTTATATATTTTTTTATTTGTTTTTCTTAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAt                                                                         <  1:4485262/128‑1 (MQ=255)
                                            atTCTTATAAATTATATCATCTTTATCTATTTTTTTAATTTTTTATATTCAAAAATAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaaa                                                                           <  1:5429420/128‑1 (MQ=255)
                                              tCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAA‑‑TAAAAACAAACAATATTTATTAAAATTcc                                                                      >  1:833599/1‑128 (MQ=255)
                                              tCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACACAAATTAATAAAAtaac                                                                      >  1:5400825/1‑125 (MQ=255)
                                                 tataATGTATATACTCGTTATATATTTTTTTATTTGTTTATATTAACAAAAAAAAAAAAAAAAACCCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTccc                                                                     <  1:6245234/128‑1 (MQ=255)
                                                 tataAATTAGATACTATTTATATATTTTTTTATTTTTTTATCTTCAAAAATAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCt                                                                    <  2:4827969/128‑1 (MQ=255)
                                                        tataTCCTCTTGTTCTTTTTTTTTAATTTTTTATATTAAAAAATAAAAAAAAAAAATTCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtat                                                                  <  1:6086729/125‑1 (MQ=255)
                                                            accTAGTTATATATTTTTTTTATTTTTATAATTAAAAAAAAAAAAAAAAAAAACCC‑‑ATTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                           <  2:305987/127‑1 (MQ=255)
                                                            aatatattaaatatttatttattTTTTTTTTTTTAAAAATTAAAAAAAAAAAAT‑‑TAATTTAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                            <  1:2473989/117‑1 (MQ=255)
                                                              ctagttatatataTTTTTATTTTTTTATCTTCACAAAAAAAAAAAAACAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACat                                                        <  1:5848661/119‑1 (MQ=255)
                                                              atatttatatatttttttattttttTATATTAAAAAATAAAAAAAAAAAATCCCC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                       <  1:3124613/119‑1 (MQ=255)
                                                               tttttttttatttttttattttttTATATTAACAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                       <  1:5508246/120‑1 (MQ=255)
                                                               ttgttatttatttttttattttttTATCTTCACAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                      <  2:5492680/120‑1 (MQ=255)
                                                                 ggtatatatttttttattttttTATATTTAAAAAAAAAAAAAAAAAATCCCATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                                     <  2:5236276/122‑1 (MQ=255)
                                                                    atatttttttttATTTGTTTAATTAAAAAAAAAAAAAAAAAAAAAACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatt                                                  <  2:5662517/123‑1 (MQ=255)
                                                                      taaTTTTTTTATTTGTTTAAATTAAAAAAAAAAAAAAAACAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                               <  1:4422611/126‑1 (MQ=255)
                                                                        ttttttttATTTTTTTTTAATAAAAAAAAAAAAAAAAAAAAACCC‑‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                              <  1:5017227/127‑1 (MQ=255)
                                                                        atttttttatttttttATATTAAAAAATAAAAAAAAACAATCACCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                             <  1:4713289/128‑1 (MQ=255)
                                                                         tttttttatttttttaTATTAACAAATAAAAAAAAAAAATACCCTT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                             <  1:427011/128‑1 (MQ=255)
                                                                           tttttATTTTTTTATATTAAAAAAAAAAAAAAAAAAATACACTT‑‑‑AAAAATTTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaaa                                           <  2:2490181/128‑1 (MQ=255)
                                                                            ttttATTTTTTTATATAAAAAAATAAAAAAAAACAATCCC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaaa                                           <  1:1664023/128‑1 (MQ=255)
                                                                             tttATTTTTTTTTATTAAAAAAAAAAAAAAAAAAATAAAC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                                         <  1:3542851/128‑1 (MQ=255)
                                                                                aTTTTTTTATTTTAAAAAAAAAAAAAAAAAAATAACC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAg                                       <  1:128032/128‑1 (MQ=255)
                                                                                 ttttttTATATTTAAAAAAAAAAAAAAAAAAAACCC‑‑‑ATAAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                                      <  1:1595041/128‑1 (MQ=255)
                                                                                  ttttttATCTTAAAAAAAAAAAAAAAAAAAAACCC‑‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                                    <  1:529037/125‑1 (MQ=255)
                                                                                  ttttttATATTAAAAAATAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATATCACATTTTATTTTTAAAATAAGata                                    <  1:4113760/125‑1 (MQ=255)
                                                                                     ttttatattaaaaaaaaaaaaaaaaaaATACCC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                                  <  1:521521/117‑1 (MQ=255)
                                                                                       ttaataaaaaaaaaaaaaaaaaaaaATTCCC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                                <  1:2322229/119‑1 (MQ=255)
                                                                                       tatatttaaaaaaaaaaaaaaaaaaATCCCATT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGAc                               <  1:5085059/119‑1 (MQ=255)
                                                                                           ataaaaaaTAAAAAAAAAAAAATCCCTTTTAATTAATAAAAAAAAAAAAAAAATAAACTAAAAACACACTCTATTTCTAAAAATACCCTATTAAACCACATTTTATTTTTAAAATAAGATATTGACat                             <  1:3425972/123‑1 (MQ=255)
                                                                                           ataaaaaaTAAAAAAAAAAAATACAC‑‑TTATAAATTATAAAAAAAAAAAAAAAAAAACTAATTACAAAATATATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                            <  1:2627758/123‑1 (MQ=255)
                                                                                            tCAAAAATAAAAAAAAAAAATACC‑‑ATTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataat                          <  1:671262/128‑1 (MQ=255)
                                                                                               caAATAAAAAAAAAAAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata                         <  1:2164567/128‑1 (MQ=255)
                                                                                                  aataaaaaaaaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAATATTTATTAAAATTCCCTATTATACCACATTTTATTTTTAAAAAAATATATTGAAATAatatata                     >  1:6391796/4‑128 (MQ=255)
                                                                                                    taaaaaaaaaCAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACAAAAAAAAAACACAATTTCTTAAAAATACCAATTAAACCACAATTTATTTTAAAAATAAGATATTAAAATAatatatata                   >  1:5932381/2‑127 (MQ=255)
                                                                                                    aaaaaaaaaaaaATACC‑‑ATTAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatatat                    <  1:2824503/128‑1 (MQ=255)
                                                                                                     aaaaaaaaaaaaTTCCCATTAAAAATTAAAAAATAAAAAAAAAAAAAAATAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatatat                    <  2:3458387/128‑1 (MQ=255)
                                                                                                     aaaaaaaaaCAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAACACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATc                   <  1:764535/128‑1 (MQ=255)
                                                                                                         aaaaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAATATTTATTAAAATTCAAATTTATAACAAATTTTATTTTTAAAAAAATAAATTTAAaaaataattataaatta              >  2:6480724/1‑117 (MQ=255)
                                                                                                           aaaCAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAAAAATATTTCTAAAAATTCACTAAAAAACCAAATTTTAATTTTAAAATAAAATATTTACAAAaaatatataaaaaaaa            >  1:396758/1‑120 (MQ=255)
                                                                                                               aaaaacaccataaaaaataaaaaaAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatat           <  2:1326074/118‑1 (MQ=255)
                                                                                                                acaaacccctaaaaaattaaaaaAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatatat         <  2:5720784/119‑1 (MQ=255)
                                                                                                                aaatacccttaaaaattaaaaaAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatatat           <  1:5356098/120‑1 (MQ=255)
                                                                                                                  caccccttATAAATTAAAAAATAAAAAAAAAAAAAACTAAACACAAACTCTATTTCTTATAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAAtatatata        <  2:6022799/122‑1 (MQ=255)
                                                                                                                      ccttAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATAc    <  2:5553998/126‑1 (MQ=255)
                                                                                                                       cttaaaaattaaaaaAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATAc    <  1:3649180/127‑1 (MQ=255)
                                                                                                                       attaaaaattaaaaaAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACt   <  1:1757150/127‑1 (MQ=255)
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TAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAATACTA  >  CP016816/202367‑202614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: