BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.5%	7897.6 / NA	2496	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (6/6); new base T (1327/1156); total (1333/1163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.9%	7549.0 / NA	2496	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (2/26); new base T (1331/1137); total (1333/1163)
Fisher's exact test for biased strand distribution p-value = 2.61e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2608 total aligned reads displayed.

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285

||

      aattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATttttaatttt                                                                                                                             >  2:2287447/1‑126 (MQ=255)

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATttttaatttta                                                                                                                            >  1:5003150/1‑125 (MQ=255)

         tcattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATAATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATttttaattttaat                                                                                                                          <  2:5727451/128‑6 (MQ=255)

          cattcatACTTTTAGTCTCATAAATCTCAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATa                                                                                                                         <  1:3223313/128‑1 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAAc                                                                                                                       >  2:5440026/1‑128 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATATTTAATTTTAATAAc                                                                                                                       >  1:2081214/1‑128 (MQ=255)

             tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACt                                                                                                                      >  1:3449910/1‑128 (MQ=255)

                tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGaa                                                                                                                   <  1:5354503/128‑1 (MQ=255)

                tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGaa                                                                                                                   <  2:6345036/128‑1 (MQ=255)

                 aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAat                                                                                                                  <  1:356858/128‑1 (MQ=255)

                  cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAatt                                                                                                                 <  2:2531824/128‑1 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAatta                                                                                                                <  2:6090940/128‑1 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAatta                                                                                                                >  2:532970/1‑128 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAatta                                                                                                                <  1:5827089/128‑1 (MQ=255)

                    tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAattat                                                                                                               >  2:2990858/1‑128 (MQ=255)

                     ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTATTTTTAATAACTGAattatt                                                                                                              >  2:2423154/1‑128 (MQ=255)

                     ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAattatt                                                                                                              <  1:5936736/128‑1 (MQ=255)

                      tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTAttt                                                                                                             <  1:1970779/128‑1 (MQ=255)

                          ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                         <  2:2804995/128‑1 (MQ=255)

                           tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                        <  1:2397395/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                      >  2:346473/1‑128 (MQ=255)

                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                     <  1:553220/128‑1 (MQ=255)

                               aaaTCTAAGTTTCTGAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                    >  2:1025980/1‑128 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                   >  1:6415941/1‑128 (MQ=255)

                                    tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                               <  2:1548225/128‑1 (MQ=255)

                                    tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                               <  1:5257483/128‑1 (MQ=255)

                                     aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAAATACTAGAAATTTTTT‑AAAAA‑ATTTTTAATTTTAATAAATTAATTATTTTTAAAATTGTTatat                                                                                              >  2:1651215/1‑128 (MQ=255)

                                      aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAATACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                             <  2:4069667/128‑1 (MQ=255)

                                      aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                             <  2:5104217/128‑1 (MQ=255)

                                      aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                             <  2:3152084/128‑1 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAActta                                                                                            >  2:5937770/1‑128 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                            >  1:6317622/1‑128 (MQ=255)

                                        tttattaaaTTATTTTATTATTTTAAAAAATTATAATTTTTTTAGTTTTTTTTTTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                           <  2:522349/122‑1 (MQ=255)

                                        tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                           <  2:5003150/128‑1 (MQ=255)

                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATttttaatt                                                                                                                               >  1:3828289/1‑91 (MQ=255)

                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTATAATTTTAATAACTGAATTATTTTTCAAATTTTTAAattatt                                                                                          >  1:4108929/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                        <  1:4577537/128‑1 (MQ=255)

                                                 ttattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATc                                                                                  >  1:1876801/1‑128 (MQ=255)

                                                    ttttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                <  2:849375/128‑1 (MQ=255)

                                                     tttATAATTTAAAATAATAATACTTTTATTAGTTGATATATTAGAAAACTTCTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                              <  2:1237073/128‑1 (MQ=255)

                                                       tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                            <  1:2761547/128‑1 (MQ=255)

                                                         tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                          <  1:163109/128‑1 (MQ=255)

                                                          cATTTAAAAAAATAATTCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑TTTTTTAATTTTAATATCTGATTTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                         <  2:3896201/128‑1 (MQ=255)

                                                          cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                         >  1:2963246/1‑128 (MQ=255)

                                                             ttAATAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCTCTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTTTTaa                                                                      <  2:4298863/128‑1 (MQ=255)

                                                             ttAAAAAAATAAGACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                      >  2:5519851/1‑128 (MQ=255)

                                                              tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                     >  1:5734104/1‑128 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                               >  2:4882935/1‑128 (MQ=255)

                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                              <  2:4723817/128‑1 (MQ=255)

                                                                          aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                         >  1:3978099/1‑128 (MQ=255)

                                                                            ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATtctct                                                       >  1:1002581/1‑127 (MQ=255)

                                                                            ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑g                                                      >  1:6220701/1‑128 (MQ=255)

                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑TAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTATATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTctt                                                      >  2:750104/1‑126 (MQ=255)

                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                   <  2:4590589/99‑1 (MQ=255)

                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑t                                                    >  2:4459595/1‑128 (MQ=255)

                                                                              ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTTAATTATTTTTCAAATTCTTAAATAATTTAAAAATCATTATCTATTAATAAAATTTAttatcttt                                                     >  1:2637036/1‑121 (MQ=255)

                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑ttt                                                  >  1:5064885/1‑128 (MQ=255)

                                                                                   actttatatatTAGAAAACTAATATAACTTTTTT‑AAAAA‑ATTTTAAATTTTAATAATATAATTATTTTTAAAATTTTTAAATTATTTTCAAATCATTATCTATTAATAAATTTTATGCTC‑G‑ttttttt                                              <  1:4755767/123‑1 (MQ=255)

                                                                                      tGATAAATTACAAAACAACTAGCAATATTTT‑AAAAA‑ATTTTTAATTTTAAAAACTGAATTATTTTTCAAATTGTTAAAATATTTTCAAATCAATATCTATTAATCAATTTTATGCGC‑G‑tttttttttt                                           <  1:5613451/128‑1 (MQ=255)

                                                                                        atatatTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑tttttttttttt                                         >  1:6507168/1‑128 (MQ=255)

                                                                                        atatatTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑attttttttttt                                         >  1:3097325/1‑128 (MQ=255)

                                                                                          atatTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTAttt                                                                                                             >  1:6590714/1‑60 (MQ=255)

                                                                                          atatTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                        >  2:4189479/1‑81 (MQ=255)

                                                                                             ttATAAAAATAATATCAATTTTTT‑AAAAA‑ATTTTTAATTTTAATAAATTAATTATTTTTAAAATTGTTAAATTATTTTAAAATAATTATCTATTAATAAATTTTATGCTC‑G‑tttttttttttttttta                                    <  2:6475290/128‑2 (MQ=255)

                                                                                             ttAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑t‑tttttttttttttttaa                                    >  2:376942/1‑126 (MQ=255)

                                                                                              tataaaaATAATAGAAATTTTTT‑AAAAA‑ATTTTTAATTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTATTTTAAAATAATTTTATATTAATAAATTTTTTGTTT‑G‑tttttttttttttttaca                                   <  2:1739296/125‑4 (MQ=255)

                                                                                               aGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGa                                                                                                                    <  1:4237480/48‑1 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑ttttttttttttttttttaaaa                               >  1:5140501/1‑123 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAaaaa                               >  2:6564459/1‑128 (MQ=255)

                                                                                                   aactaatataaatttttt‑AAAAA‑ATTTTTAATTTTAATAAATTTATTTTTTTTTAAATTTTTAAATTATTTTAAAATAATTATATATTAATCAATTTTATTCTCTT‑TTTTTTTTTTTTTTTTTACaaa                               <  1:229811/116‑1 (MQ=255)

                                                                                                   aaaaaatatcaatttttt‑AAAAA‑ATTTTTAATTTTAATAAATTAATTATTTTTTAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTATGCTC‑GTTTTTTTTTTTTTTTTTTACaaa                               <  1:4882041/116‑1 (MQ=255)

                                                                                                   aaCTACTAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTC‑G‑TTATTTTATATTTTTTTaaaaaa                              >  1:2852835/1‑128 (MQ=255)

                                                                                                    actactAGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTTaaaaaa                             >  1:2199311/1‑128 (MQ=255)

                                                                                                    aattatataatattttt‑AAAAA‑ATTTTTAATTTTATAAATTTTATTATTTTTTAAATTTTTAAATTTTTTTTAAATAATTATTTTTTAATAAATTTTATTGTC‑G‑TTTTTTTTTTTTTTTTTACaaaaa                             <  2:2116060/116‑1 (MQ=255)

                                                                                                     attatataaatttttt‑AAAAA‑ATTTTTAATTTTAATAAATTTATTTTTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTTTTCTC‑GTTTTTTTTTTTTTTTTTTACaaaaa                             <  1:1407699/118‑1 (MQ=255)

                                                                                                        ctaGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑T‑TTTTTTTTTTTTTTTTTAAAAAAataaa                         >  1:433653/1‑126 (MQ=255)

                                                                                                         taGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTTTaaaaaattat                        >  1:1301820/1‑123 (MQ=255)

                                                                                                         taGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATATaa                        >  2:5574944/1‑128 (MQ=255)

                                                                                                         taGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑‑TTTTTTTTTTTTTTTTACAAAAATATAat                       >  2:5659966/1‑128 (MQ=255)

                                                                                                          ttaaatttttt‑AAAAATATTTATATTTTAAATAA‑TTTTTTTTTTTTCAAATATTTAATTTTTTTTAAAATCAATTTATATTAATTAATTTTATTCTCTT‑TTTTTTTTTTTTTTTTTACAAAAATATaa                        <  2:2027254/123‑1 (MQ=255)

                                                                                                          ttaaatttttt‑AAAAA‑ATTTTTAATTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTTTTTTAAAATTATTATTTATTAATAAATTTTTTTATC‑GTTTTTTTTTTTTTTTTTTACAAAAATATaa                        <  2:3598301/123‑1 (MQ=255)

                                                                                                          aGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑ttttttttttttttacaaa                                  >  1:3686877/1‑112 (MQ=255)

                                                                                                          aGCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTAaaaaaaaaaaattt                       >  1:2503308/1‑122 (MQ=255)

                                                                                                           gCACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAat                       >  2:1407699/1‑128 (MQ=255)

                                                                                                            cACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTACAAAAAAAAAattt                     >  2:1910159/1‑128 (MQ=255)

                                                                                                            cACTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATAAAattt                     >  2:2665120/1‑128 (MQ=255)

                                                                                                             attttttt‑AAAAA‑ATTTTTAATTTTAATAAATTTATTTTTTTTAAAATTTTTAAATTATTTTTAAATTATTATATTTTTATTAATTTTATTGTC‑T‑TTTTTTTTTTTTTTTTTACAAAAATATAattta                    <  1:4459595/126‑1 (MQ=255)

                                                                                                             aCTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAattt                     >  1:1136701/1‑128 (MQ=255)

                                                                                                              cTTTTTT‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCTT‑TTTTTTTTTTTTTTTTTAAAAAAATAT‑atttat                   >  2:5834810/1‑128 (MQ=255)

                                                                                                               tttttt‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAaaaaaaataaacgaaat                  >  1:155426/1‑117 (MQ=255)

                                                                                                               tttttt‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATATAatttatt                  >  1:3365674/1‑128 (MQ=255)

                                                                                                               tttttt‑AAAAA‑ATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑‑TTTTTTTTTTTTTTTTACAAAAATATAatttattt                 >  1:173098/1‑128 (MQ=255)

                                                                                                                  ttt‑AAAAA‑ATTTTTAATTTTAATAAATTAAATATTTTTTAAATTTTTAAATTATTTTTAAAAAATTTTATATTAATAAATTTTATGCTC‑GTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTg                <  2:683278/128‑1 (MQ=255)

                                                                                                                   tt‑AAAAA‑ATTTTTAATTTTAAAAACTGAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTC‑G‑‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt             <  2:6210367/128‑1 (MQ=255)

                                                                                                                     tataaa‑aTTTTTAATTTTAAAAAATTAATTATTTTTTAAAATTTTAAATTATTTTAAAATAAATATATATAAATAAATTTTATTCTC‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt             <  1:697174/125‑1 (MQ=255)

                                                                                                                       taaa‑aTTTTTAATTTTAATTACAGATTTATTTTTAAAATTTTTAAATTATTTTTAAAAAAATTTAAAATTATAAATTTTAATTGC‑G‑‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaaa          <  1:5435172/127‑1 (MQ=255)

                                                                                                                        aaa‑aTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑T‑TTTTTTTTTTTTTATTTAAAAAAAAATAATTAATTTTATTaaa          >  1:84262/1‑128 (MQ=255)

                                                                                                                        aaa‑aTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAAAAAAAAATATTTTATTTTATaaaa          >  1:6051926/1‑128 (MQ=255)

                                                                                                                        aaa‑aTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑‑TTTTTTTTTTTTTTTTACAAAAATAAAATTAATTTTATTAAAc         >  2:3370300/1‑128 (MQ=255)

                                                                                                                         aa‑aTTTTTAATTTTAATAACTGAATTATTTTTAAAATTGTTAAATTATTTTAAAATAATTATATATTAATAAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAAc         <  1:6143163/128‑1 (MQ=255)

||

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTT‑AAAAA‑ATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`