BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,163	2 bp→TT	intergenic (‑446/+396)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,163	0	A	T	99.5%	4030.5 / NA	1285	intergenic (‑446/+397)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (3/4); new base T (704/568); total (708/577)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,164	0	A	T	98.7%	3613.4 / NA	1276	intergenic (‑447/+396)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (4/13); new base T (697/562); total (701/575)
Fisher's exact test for biased strand distribution p-value = 1.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1287 total aligned reads displayed.

TCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420040‑420281

||

 cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATa                                                                                                                             <  2:2440177/128‑1 (MQ=255)

 cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATa                                                                                                                             <  2:2053037/128‑1 (MQ=255)

 cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATa                                                                                                                             <  1:1457796/128‑1 (MQ=255)

   ttcttACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAAc                                                                                                                           <  2:2241378/128‑1 (MQ=255)

   ttcatACTTTTAGTCTCATAAATATAAGTTTCTAAAATTATTTTATCATTTAAAAATATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAAc                                                                                                                           <  1:936759/128‑1 (MQ=255)

   ttcatACTTATAGGCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAAc                                                                                                                           <  1:523621/128‑1 (MQ=255)

    tcatACATTTAGTCTCATAAAACTAAGTTTCTAAAATTATATTATCATTAAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACt                                                                                                                          <  2:1154454/128‑1 (MQ=255)

      atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGa                                                                                                                        <  1:2804857/128‑1 (MQ=255)

       tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGaa                                                                                                                       <  2:1893317/128‑1 (MQ=255)

        aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAat                                                                                                                      >  2:1804326/1‑128 (MQ=255)

         cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAatt                                                                                                                     >  2:3601539/1‑128 (MQ=255)

          ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAatta                                                                                                                    >  1:2894717/1‑128 (MQ=255)

            ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAatta                                                                                                                    <  1:1064120/126‑1 (MQ=255)

             tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTAttt                                                                                                                 <  2:569977/128‑1 (MQ=255)

              aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTAtttt                                                                                                                <  2:3330779/128‑1 (MQ=255)

                tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                              >  2:3273825/1‑128 (MQ=255)

                  tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            <  1:2981295/128‑1 (MQ=255)

                  tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            >  1:3286244/1‑128 (MQ=255)

                    aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          >  1:3122257/1‑128 (MQ=255)

                    aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  2:288551/128‑1 (MQ=255)

                    aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  2:3062551/128‑1 (MQ=255)

                    aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  1:1358854/128‑1 (MQ=255)

                    aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  1:2601229/128‑1 (MQ=255)

                    aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  2:353515/128‑1 (MQ=255)

                      aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                        >  1:815235/1‑128 (MQ=255)

                      aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                        <  1:119278/128‑1 (MQ=255)

                        aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                      >  1:3344716/1‑128 (MQ=255)

                        aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                      <  2:2296642/128‑1 (MQ=255)

                         tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTATAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                     >  1:1268411/1‑128 (MQ=255)

                          atAATTTTCTAAAATTATTTTATCATTTATAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaa                                                                                                    <  2:1018357/127‑1 (MQ=255)

                           tAAGTTTCTAAAATTATTTTATCATATAAAAATATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                   <  1:217521/128‑1 (MQ=255)

                             aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                 <  2:3749250/128‑1 (MQ=255)

                             aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                 <  1:3618309/128‑1 (MQ=255)

                             aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                 >  1:1957944/1‑128 (MQ=255)

                               tttCTAAAATTATTTTATCATTTAAAAAAATTATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAATAAA‑TTTTTAATTTTAATAACTGAATTAATTTTCAAATTGTTAAattat                                                                                               <  1:3842444/128‑1 (MQ=255)

                                ttCTAATATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                              <  2:2641319/128‑1 (MQ=255)

                                ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                              >  1:3664002/1‑128 (MQ=255)

                                ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                              >  2:2778576/1‑128 (MQ=255)

                                 tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                             <  1:3445626/128‑1 (MQ=255)

                                 tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                             <  2:1322598/128‑1 (MQ=255)

                                  cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                            >  1:2411642/1‑128 (MQ=255)

                                   tAAAATTATTTTATCAATTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                           <  1:772679/128‑1 (MQ=255)

                                    aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                          <  1:3530428/128‑1 (MQ=255)

                                      aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                        >  1:3170065/1‑128 (MQ=255)

                                        ttattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATc                                                                                      <  1:3063307/128‑1 (MQ=255)

                                         tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                     >  1:881127/1‑128 (MQ=255)

                                               aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               <  1:2856770/128‑1 (MQ=255)

                                               aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               >  2:3826678/1‑128 (MQ=255)

                                               aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               >  1:3387392/1‑128 (MQ=255)

                                                tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                              <  1:2674542/128‑1 (MQ=255)

                                                 cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                             >  1:64501/1‑128 (MQ=255)

                                                 cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                             >  1:3734638/1‑128 (MQ=255)

                                                 cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                             >  2:1245491/1‑128 (MQ=255)

                                                  aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                            >  1:2470119/1‑128 (MQ=255)

                                                   tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                           >  2:788025/1‑128 (MQ=255)

                                                     tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                         >  2:3126292/1‑128 (MQ=255)

                                                       aataaaTAATACTTTTATTAGTTGATATATTAGTATACTACTAGCACTTTTTTAAAAAA‑TTTTTATTTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                       <  2:2943199/125‑1 (MQ=255)

                                                        aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa‑‑a                                                                    <  2:1072330/128‑1 (MQ=255)

                                                        aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCAATTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTTTTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCa‑‑a                                                                    <  2:867390/128‑1 (MQ=255)

                                                         aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTATATTATTTTCAAATCATTATCTATTAATCA‑‑At                                                                   <  1:2302239/128‑1 (MQ=255)

                                                           aataatACTTTTATTAGTTGATATATTAGTAAACTTCTAGCACTTTTTTTAAAAA‑ATTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑Attt                                                                 <  1:1410493/128‑1 (MQ=255)

                                                           aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑Attt                                                                 <  2:33909/128‑1 (MQ=255)

                                                           aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑Attt                                                                 <  2:474955/128‑1 (MQ=255)

                                                            ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑Atttt                                                                <  1:567579/128‑1 (MQ=255)

                                                              attaCTTTTATTAGTTTATATATTAGAAAAATAATAGCAATTTTTTAAAAAA‑TTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑t                                                            <  2:1497499/126‑1 (MQ=255)

                                                              aataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑t                                                            <  1:2067020/128‑1 (MQ=255)

                                                                taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑c                                                        <  1:1000360/128‑1 (MQ=255)

                                                                 aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TT‑‑Ct                                                       >  1:2137505/1‑128 (MQ=255)

                                                                   ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATAATTTTTCAAATAGTTAAATTATATTCAAATCATTATCTATAAATAA‑‑ATTTTA‑‑Tt‑‑ctct                                                     >  1:1809311/1‑127 (MQ=255)

                                                                    tttATTAGTTTATAAATTAGAAAACTACTACCACTTTTTTAAAAAA‑TTTTTAATTTTAATAAATGAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CGC‑G‑‑t                                                 <  1:1716848/128‑1 (MQ=255)

                                                                    tttATTAGTTGATATGTTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑t                                                 >  1:3581560/1‑128 (MQ=255)

                                                                    tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TT‑‑CTC‑G‑‑t                                                 >  1:3788094/1‑128 (MQ=255)

                                                                      tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTAtttt                                                                                                                <  2:3687773/72‑1 (MQ=255)

                                                                      tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑ttt                                               >  2:928455/1‑128 (MQ=255)

                                                                      tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑ATC‑t‑‑ttt                                               >  1:2729404/1‑128 (MQ=255)

                                                                        ttattttaaataataTAAAACTAAAATAACTTTTTTAAAAAA‑TTTTTAAATTTAATAAATTAATTATTTTTAAAATTTTTTAATTATTTTAAAATAATTATCTATTAATCA‑‑ATTTTT‑‑TT‑‑CTC‑t‑‑ttttt                                             <  2:3180271/119‑1 (MQ=255)

                                                                        ttaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                   >  2:2146192/1‑83 (MQ=255)

                                                                        ttaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATAATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑T‑‑ttatt                                             >  1:706498/1‑128 (MQ=255)

                                                                              tttatatTATAAAAATAATATTAATTTTTTAAAAAA‑TTTTTTATTTTAATAAATTAATTATTTTTCAATTTTTTAAATTATTTTCAAATCATAATCAATTAATCA‑‑ATTTTA‑‑TG‑‑CCC‑G‑‑ttttttttt‑tt                                      <  1:1637885/126‑1 (MQ=255)

                                                                                tatatTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATATTAATCTATTAATCA‑‑ATATTA‑‑TT‑‑ATC‑G‑‑atttttttt‑tttt                                    >  1:2022512/1‑128 (MQ=255)

                                                                                    ttAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAGTTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑ttttttttt‑tttttttt                                >  2:1581191/1‑128 (MQ=255)

                                                                                     tAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTTTTTT‑TTTTTTTTa                               >  1:3484908/1‑128 (MQ=255)

                                                                                           actactAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTTTTTT‑TTTTTTTTTTaaaaa                         >  2:1388914/1‑128 (MQ=255)

                                                                                             taaattgaattttttAAAAAATTTTTAATTTTAAATAA‑TTAATTATTTTTAAATTTTTTATATTTTTTTAAAATAATTATATATTAATAA‑‑ATTTTA‑‑TG‑‑GTC‑GT‑TTTTTTTTT‑TTTTTTTTACAAAAat                        <  2:1996510/119‑1 (MQ=255)

                                                                                                 aGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTTTTTT‑TTTTTTTTTaaaaaaattata                   >  1:2237457/1‑122 (MQ=255)

                                                                                                 aGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATAATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTTTTTT‑TTTTTTTTTTaaaaaaaaaaa                   >  1:1937102/1‑122 (MQ=255)

                                                                                                  ttaCTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATATATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑GT‑TTTTTTTTT‑TTTTTTTTACAAAAATATAat                   <  1:3230085/126‑1 (MQ=255)

                                                                                                   tatttttttTAAAAA‑TTTTTTATTTTATTAAATTTATTTTTTTTTAAATTTTTAAATTTTTTTAAAATTATTTTTTTTTAATAATTTTTTTT‑‑TT‑‑TTT‑T‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAat                   <  2:164502/125‑1 (MQ=255)

                                                                                                   atgatttttAAAAAT‑TTTTAAATT‑TAATAACTTAATTATTTTTGAAAATTTTAAAATATTTTTAAATAAATATTAATTAATAA‑‑AATTTA‑‑TT‑‑GGC‑G‑‑‑GTTTTTTT‑TTTTTTTTACAAAAATATAatttat               <  1:2533040/124‑1 (MQ=255)

                                                                                                    aCTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTTTTTT‑TTTTTTTTAAAAAAAAATAattta                >  1:403268/1‑128 (MQ=255)

                                                                                                      ttttttAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTTTTTT‑TTTTTTTTTAAAAAAAAAAaattaat              >  1:1113380/1‑126 (MQ=255)

                                                                                                      ttttttAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTTTTTT‑TTTTTTTTAAAAAAATATAatttatt              >  1:152281/1‑128 (MQ=255)

                                                                                                       ttttaAAATAA‑TTTTTAATTTTAATAAATTTATTATTTTTAAAATTGTTTAATTATTTTCAAATAATTATATATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑GTTTTTTTTTTT‑TTTTTTTTACAAAAATATAatttat               <  1:460245/128‑1 (MQ=255)

                                                                                                          tttaaaaa‑TTTTTAATTTTAATAAATTTATTATTTTTAAAATTTTTAAATTTTTTTTAAATAATTTTTTTTTAATAA‑‑ATTTTATTTT‑‑CTT‑T‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAATTTATTTg            <  2:3880110/125‑1 (MQ=255)

                                                                                                          ttAAAAAA‑TTTTTAATTTTAATATCTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TGATCTT‑T‑‑TTTTTTTTT‑TTTTTTTTAAAAAAATATAatttatttt            >  2:3485219/1‑127 (MQ=255)

                                                                                                           tAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑GT‑TTTTTTTTT‑TTTTTTTTAAAAAAAAATTAtttattttat          >  2:713394/1‑128 (MQ=255)

                                                                                                            aaaaaa‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTGAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTCAT‑‑TTTTTTTTT‑TTTTTTTTAAAAAAATATAATttattttatt         >  2:195673/1‑128 (MQ=255)

                                                                                                             aaaaa‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTATTTT‑TTTTTTTTAAAAAAATATAATTATATTGATTaa       >  1:1868390/1‑128 (MQ=255)

                                                                                                              aaaa‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑GT‑TTTTTTTTT‑TTTTTTTTAAAAAAATAAAATTTATTTTATTaa       >  1:1588387/1‑128 (MQ=255)

                                                                                                              aaaa‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCAATTAATCA‑‑AAATTA‑‑TA‑‑CTC‑T‑‑TTTTTTTTTATTTTTTTAACTAAATTATAatttatattaataa       >  2:2547018/1‑120 (MQ=255)

||

TCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTA‑‑TG‑‑CTC‑G‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420040‑420281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`