BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	165,222	G→A	intergenic (+186/‑356)	JCVSYN2_00665 → / → JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	165,222	0	G	A	98.8%	7460.2 / 55.5	2420	intergenic (+186/‑356)	JCVSYN2_00665/JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (1377/1006); minor base . (4/25); total (1382/1038)
Fisher's exact test for biased strand distribution p-value = 1.57e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2488 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATAC  >  CP016816/165097‑165351

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTATAAAAaat                                                                                                                                  >  1:4728421/1‑128 (MQ=255)

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTAATTACTTTATCGCACTTGTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaat                                                                                                                                  <  2:4886449/128‑1 (MQ=255)

   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaatta                                                                                                                                <  2:1857011/128‑1 (MQ=255)

   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaatta                                                                                                                                <  2:6865184/128‑1 (MQ=255)

   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATATTTTGTATATGCAAA‑TTTTTAAAAaatta                                                                                                                                >  1:2516684/1‑128 (MQ=255)

    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaataaa                                                                                                                               >  1:3902349/1‑128 (MQ=255)

       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAAttt                                                                                                                            >  2:5597946/1‑128 (MQ=255)

       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAAttt                                                                                                                            <  1:4057303/128‑1 (MQ=255)

        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTg                                                                                                                           >  1:922223/1‑128 (MQ=255)

        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TATTTAAAAAATTAATTTg                                                                                                                           >  2:2953881/1‑128 (MQ=255)

          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGaa                                                                                                                         >  2:5348281/1‑128 (MQ=255)

           ttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAAc                                                                                                                        >  1:1982260/1‑128 (MQ=255)

           ttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAAc                                                                                                                        >  1:5263241/1‑128 (MQ=255)

              ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTg                                                                                                                     <  1:1007831/128‑1 (MQ=255)

              ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTg                                                                                                                     <  2:4991043/128‑1 (MQ=255)

              ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTg                                                                                                                     <  1:7163138/128‑1 (MQ=255)

               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGt                                                                                                                    >  1:1327927/1‑128 (MQ=255)

                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTATATATTTTTATTATATTCAAA‑TTAATAAAAAAATAATTAGAACtatta                                                                                                                   >  2:5250492/1‑124 (MQ=255)

                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                   >  2:6252143/1‑128 (MQ=255)

                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACAATTATATTTTTAGATATATATTTTATATGCAAA‑TATTAAAAAAATTAAATTGAACTTGTa                                                                                                                   >  1:4628450/1‑128 (MQ=255)

                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                  <  2:4769938/128‑1 (MQ=255)

                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                  <  2:3818117/128‑1 (MQ=255)

                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                                 <  2:2068880/128‑1 (MQ=255)

                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                              <  2:7186003/128‑1 (MQ=255)

                      gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAAt                                                                                                             >  1:6325627/1‑128 (MQ=255)

                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                            >  1:3761037/1‑128 (MQ=255)

                               atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttt                                                                                                    >  2:5935748/1‑128 (MQ=255)

                                tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttt                                                                                                   >  1:1727871/1‑128 (MQ=255)

                                 ttATTTTTATTAAATTATTCCACTTTTATTAATTAGTAATAAACTAAAAAAATTATCATTTTATATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAAAAGCtttttttt                                                                                                  <  1:4464700/128‑1 (MQ=255)

                                  tAGTATCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                                 >  1:5405206/1‑128 (MQ=255)

                                    gtttattttaTTTATTGCAATTTTTTGAATTATTAATAAAATAAAACTATTATCTTTTTTTTTATTTTTTGTATATTCAAA‑TTTTTAAAAAATTTATTTTAACTTGTAAAAAAAAGCttttttttttt                                                                                               <  1:6651431/119‑1 (MQ=255)

                                      ttCATTAATTTATAGAACTTTTATGTATTATTAAGAAAATAAAAATATTATATTTTTAGATATTTTTTGTAAATGCAAA‑TTTTTAAAAAATTAAATTGAACTTGTAAAAAATAGCttttttttttttt                                                                                             <  2:5709206/128‑1 (MQ=255)

                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                            >  1:2350549/1‑128 (MQ=255)

                                        caatAAATTATCGAACTTTTAAGAATTTGTAAGAAAATAAAACTATTATCTTTTTATATATTTTTTTTATATGCAAA‑TTTTTAAAAAATTAAATTGAACTTGTAAAAAAAATCttttttttttttttt                                                                                           <  2:6651641/125‑1 (MQ=255)

                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttattt                                                                                           >  1:2077765/1‑128 (MQ=255)

                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttta                                                                                           >  1:7056904/1‑127 (MQ=255)

                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                          >  2:3957404/1‑128 (MQ=255)

                                               ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCatttttatttttttttaaaatt                                                                                    >  1:2198032/1‑122 (MQ=255)

                                                ttttgCACTTGTTTGTATTTATTATAAAAAAAAAAAATTATATTTTTTGATATTTTTTTTTTAATCAAA‑TTTTTAAAAAATTTAATTGAAAATTTAAAAAAAAGCttttttttttttttttataatgg                                                                                   <  2:5822400/124‑8 (MQ=255)

                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑TTATTTTTTTATTTTTTTATAATTTAAatat                                                                          >  2:1294969/1‑128 (MQ=255)

                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTaatattatattat                                                                           >  1:1475440/1‑119 (MQ=255)

                                                          gtatgtatTAGTATTAAAATAAAAATTTTATATATTTATATATTTTTTGTATATGCAAA‑TTTTTAATAAATTAATTTTTAATTGTAAAAAAATGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAt                                                                      <  2:2845639/128‑1 (MQ=255)

                                                            aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATATAAAtttt                                                                   >  2:2649905/1‑128 (MQ=255)

                                                            aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAAtttt                                                                   >  1:6789737/1‑128 (MQ=255)

                                                                aTTAGTAAGAAAAAAAAACTAATTTAGTTTTATTTATTTTTTGTTTATTCAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑gg                                                               <  2:4585017/128‑1 (MQ=255)

                                                                 tttttaataaAATAAAAATATTTATTTTTTTTTTATTTTTTGATTATGAAAA‑TTTTTAAAAAATAAATTTTAAATTTTAAAAAAT‑‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGt                                                              <  1:1874751/124‑1 (MQ=255)

                                                                 ttattaATAAAATAAAAATATTATTTTTTTATATATTTTTTGTTTTTTCAAA‑TTTTTTAAAAATTAATTTTTACTTGTAAAAAAAA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGt                                                              <  1:3198188/128‑1 (MQ=255)

                                                                 ttattaAGTAATTAATATTTTTTTCTTTTTTTTTATTTTTTGTATATTTAAA‑TTTTTAAAAAATTAATTTGTACTTGTAAAAAATA‑‑‑‑‑TGTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTAt                                                            <  2:35303/128‑1 (MQ=255)

                                                                  tattaataaAATAAAAATATTATTTTTTTATATTTTTTTTTTTTATTAAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTa                                                             <  2:6961542/125‑1 (MQ=255)

                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATATAAATTTTatttat                                                             >  2:4271569/1‑123 (MQ=255)

                                                                    ttaataaaaaaaaaataTTAATTTTTTATTTATTTTTTTTATATTCAAA‑TTTTTAAAAAAATAATTTTTAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATGAATTTTA‑GGTAtt                                                           <  1:5856646/118‑1 (MQ=255)

                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTA‑GTTAtt                                                           >  1:5582124/1‑128 (MQ=255)

                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATTAT‑‑T‑TAAATATAAATTTTattttattt                                                          >  2:7086799/1‑120 (MQ=255)

                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATATAAATTTTA‑TTTAttttt                                                        >  1:2673193/1‑127 (MQ=255)

                                                                        taaaataaaacttTTTTTTTTTTTTATATTTTTTGTTTATTCAAA‑ATTTTAAAAAATTAATTTGAAATTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTttgt                                                       <  2:6541618/127‑1 (MQ=255)

                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTA‑TTTATTttgttt                                                     >  1:4208803/1‑127 (MQ=255)

                                                                            ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATAAAATTTTTA‑GTTATTTTTTTTTc                                                   >  1:1572114/1‑128 (MQ=255)

                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTA‑GGTTTTTttttgtat                                                  >  1:5022075/1‑126 (MQ=255)

                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTA‑TGTATTTTTTTGtata                                                 >  2:2494248/1‑128 (MQ=255)

                                                                             aaaaactaTTATATTTTTATATTTTTTTTTTTTATGCAAA‑TTTTTAAAAAATTAAATTTAAATTGTTAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCt                                                  <  2:3188386/127‑1 (MQ=255)

                                                                             aaaaaataTTTTATTTTTTGTTATTTTTTTTTTTTGCAAA‑TTTTTAAAAAATTATTTTTTACTTGTAAAAAAT‑‑TTCGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTc                                                   <  1:3612157/127‑1 (MQ=255)

                                                                              aaaattaTTATCTTTTTATATAATTTTTGTATATTCAAA‑TTTTTAAAAAATTAATTTGTACTTTTAAAAAAT‑‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTa                                                 <  2:4683512/128‑1 (MQ=255)

                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GTTATTTTTTTGTcaa                                                 >  1:3072311/1‑126 (MQ=255)

                                                                              aaaactaATTTATTTTTATATATTTTTTTTATATTAAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAAT‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTa                                                 <  2:3682493/128‑1 (MQ=255)

                                                                               aaaattttatATTTTTATTTATTTTTTGTATATTCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTaa                                                <  1:2894805/122‑1 (MQ=255)

                                                                                   ttttatatttttttatatttttTTTTTATTTAAA‑TTTTTTAAAAATTATTTTTAAATTGTTAAAAAT‑‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAt                                            <  2:332494/126‑1 (MQ=255)

                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTA‑GGTATTTTGTTGTATaatttt                                            >  2:1576607/1‑124 (MQ=255)

                                                                                    attatATTTTTATATATTTTTTGTATATTCAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAt                                            <  2:3429559/128‑1 (MQ=255)

                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTAT‑‑‑TAATGTAAATAAAAATTTTA‑TTTATtttttttaaaaagtatat                                          >  1:3476595/1‑116 (MQ=255)

                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATAAT‑‑TGGAAATAAAAAATTTA‑TTTATTTTTTTTTATaattttat                                          >  2:785310/1‑122 (MQ=255)

                                                                                        tCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                   >  1:1147771/1‑56 (MQ=255)

                                                                                        tCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                   <  2:1147771/56‑1 (MQ=255)

                                                                                         ttttttATTTATTTTTTTTTTTTTTAAATTTTTTAAAAATTTAATTTTAA‑TTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTaaaa                                     <  2:2229992/127‑1 (MQ=255)

                                                                                           ttttATATTTTTTTTTTTTATGTAAA‑TTTTTAAAAATTTAATTTTAATTTTTAAAAAATA‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTATAAGTAT‑CTaaaaaaa                                  <  1:5131012/128‑1 (MQ=255)

                                                                                            tttATATTTTTTTTGTTTTTTAAAA‑TTTTATAAAAATTATTTTTAACTTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTaaaaaaa                                  <  2:2350549/128‑1 (MQ=255)

                                                                                            tttATATATTTTTTGTATATGGAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAAAAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTaaaaaaa                                  <  2:6758951/128‑1 (MQ=255)

                                                                                            tttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTTA‑GTTTTTTTTTTTTCAAATTAT‑ATaaaaaaa                                  >  2:5447813/1‑128 (MQ=255)

                                                                                              ttttatttttttttttttGCAAA‑TTTTTTAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAg                               <  2:6383329/122‑1 (MQ=255)

                                                                                                   tttttttttATTTGAAAA‑TTTTTAAAAAATTAATTTGTAATTTTAAAAAATTGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAg                           <  1:6085018/127‑1 (MQ=255)

                                                                                                     tttttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑‑‑ATGAAATTATAAATTTTA‑GGTATTTTGTTTTATAATTAT‑ATAAAAAAATAGAAAgta                         >  1:784038/1‑126 (MQ=255)

                                                                                                      ttttttATATTTAAA‑TTTTTTAAAAATTAATTTTAAATTTTAAAAAA‑‑‑‑TTTGTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGaaa                       <  2:3082520/128‑1 (MQ=255)

                                                                                                      ttatttctatttaaa‑TTTTTAAAAAATTTATTTTAAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGaa                        <  1:6501461/116‑1 (MQ=255)

                                                                                                       tttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGAAAAAATAAATTTTA‑GTTATTTTTTTGTCTAATTAA‑AAAAAAAAAAAAAAAGTaa                        >  1:3323418/1‑128 (MQ=255)

                                                                                                        ttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATAAAATTTTTA‑TTTATTTTTTTTTATAATTTTAATAAAAAAATaaattaaaa                        >  2:2762519/1‑120 (MQ=255)

                                                                                                        ttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATTGAATTATAAATTTTA‑GGTATTTTTTTTTCTAATTAT‑ATAAAAAAATAGAAAGGaaaaaa                    >  1:6213281/1‑128 (MQ=255)

                                                                                                         tGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGTAAATATAAATTTTA‑GGTATTTTGTTGTATAAGTAT‑ATAAAAAAATAGAAAGGaaaaaaa                   >  1:6920306/1‑127 (MQ=255)

                                                                                                           tttttttaaa‑TTTTTAAAAAATTAATTTGTAATTTTAAAAAAT‑‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAAat                   <  1:4345395/121‑1 (MQ=255)

                                                                                                             tatttata‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAAT‑‑‑TTATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAAatat                 <  2:4114550/121‑1 (MQ=255)

                                                                                                               tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTA‑GTTATTTTTTTTTATAAGTAT‑ATAAATAAATAAAAAGTAAAAAAtttt                >  2:5101363/1‑128 (MQ=255)

                                                                                                               tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTA‑GTTATTTTTTTGTATATTTAT‑ATAAAAAAATATAAATGAAAAAATAttt               >  2:4420377/1‑127 (MQ=255)

                                                                                                                 cAAA‑TTTTTATAAAATTAATTTGTACTTGTAAAAAATAGC‑‑‑‑‑‑‑TTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAAATATTGTTaaaa         <  1:4836759/128‑1 (MQ=255)

                                                                                                                  aaa‑TTTTTAAAAAATTAATTTTATCTTTTAAAATAAAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAAATATTGTTaa           <  2:6137007/128‑1 (MQ=255)

                                                                                                                  aaa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATTAAAATATAAATTTTA‑TTTTTTTTTTTTTTTATTTTT‑ATAAAAAAATTAAATGTAAAAAAtttttt              >  2:901657/1‑126 (MQ=255)

                                                                                                                  aaa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTT‑GGTATTTTTTTTTATAATTAT‑ATAAAAAATTTGAATGTATAATATATTTTTa            >  2:6069039/1‑128 (MQ=255)

                                                                                                                  aaa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTTTTGTATAAGTAT‑CTAAAAAAATATAAATGAAAAAATATTTTTaa           >  2:143242/1‑128 (MQ=255)

                                                                                                                    a‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTT‑‑‑TAATGTAAATATAAATTTTA‑GGTTTTTTGTTTTCTAAGTAT‑ATAAAAAAAAATAAATCAAAAAATATTGTTaaa          >  1:572341/1‑128 (MQ=255)

                                                                                                                      ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTT‑GGTATTTTTTTGTATATGTAT‑ATAAAAAATTAAAAAGTAAAAAATATTTTTaaa          >  2:1722915/1‑128 (MQ=255)

                                                                                                                         ttaaaaaaTTATTTTTAATTTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa      <  1:280579/126‑1 (MQ=255)

                                                                                                                         ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTA‑AATTGAAATATTAATTTTATGTTTTTTTTTTGTATATTTAA‑ATAAAAAAATAAAATGAAAAAAATATTTTTaaattt       >  2:1933489/3‑125 (MQ=255)

                                                                                                                           aaaaaaTTATATTTAAATTGTAAAAAATAGCA‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATa   <  1:5971161/128‑1 (MQ=255)

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTAT‑CTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATAC  >  CP016816/165097‑165351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`