BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,163	2 bp→TT	intergenic (‑446/+396)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,163	0	A	T	99.5%	8776.9 / NA	2826	intergenic (‑446/+397)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (8/6); new base T (1503/1298); total (1512/1314)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,164	0	A	T	99.0%	7780.6 / NA	2802	intergenic (‑447/+396)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (2/27); new base T (1496/1275); total (1499/1303)
Fisher's exact test for biased strand distribution p-value = 1.26e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2832 total aligned reads displayed.

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420038‑420287

||

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                            <  2:4639095/128‑1 (MQ=255)

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                            <  1:4937595/128‑1 (MQ=255)

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                            <  2:1672065/128‑1 (MQ=255)

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                            <  1:594513/128‑1 (MQ=255)

   aattcatACTTTTAGTATCATAAATCTAAGTTTCTAATATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                            <  1:2075902/127‑1 (MQ=255)

    attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                           <  2:1053424/128‑1 (MQ=255)

    attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                           >  1:5341714/1‑128 (MQ=255)

     ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                          <  2:3801925/128‑1 (MQ=255)

     ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                          >  2:6727282/1‑128 (MQ=255)

     ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                          >  2:2495042/1‑128 (MQ=255)

      tcatACTTTTTTTTTAATAAATCTAAGTTTATAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTTATATATTAGAAAAATACTATCAATTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                         <  1:4306113/128‑1 (MQ=255)

      tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                         <  1:2367559/128‑1 (MQ=255)

      tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                         >  1:4465817/1‑128 (MQ=255)

       catACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                        >  2:1234250/1‑128 (MQ=255)

         tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                      >  2:279693/1‑128 (MQ=255)

          aCTTTTATTCTCATAAATCTAAGTTTCTAATATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                     <  1:3618086/128‑1 (MQ=255)

          aCTTTTAGTCTCATAAACCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                     >  1:4702842/1‑128 (MQ=255)

             tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                  <  2:3443920/128‑1 (MQ=255)

             tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                  <  2:4534911/128‑1 (MQ=255)

             tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                  <  2:4936478/128‑1 (MQ=255)

             tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                  <  2:2669504/128‑1 (MQ=255)

               tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                                <  2:3956082/128‑1 (MQ=255)

               tAGTCTCATAAATCTAAGTATCTAAAATTATTTTATCAATTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                                <  2:5656267/128‑1 (MQ=255)

                    tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                           <  1:5569150/128‑1 (MQ=255)

                    tcATAAATCTAAGTTTCTAAAATTATTTAATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                           <  1:7348553/128‑1 (MQ=255)

                    taaTAAATCTAAGTTGCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                           <  2:5826781/126‑1 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                         <  1:2827505/128‑1 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                         <  1:5803627/128‑1 (MQ=255)

                         aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                      <  1:2222636/128‑1 (MQ=255)

                         aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                      >  1:769545/1‑128 (MQ=255)

                          aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTATAAAACTACTAGCAATATTTAAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaaatctt                                                                                                     >  1:2505171/1‑123 (MQ=255)

                          aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                     >  2:5151050/1‑128 (MQ=255)

                          aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                     <  2:5255341/128‑1 (MQ=255)

                           tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                    <  1:792444/128‑1 (MQ=255)

                               aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                <  1:6462308/128‑1 (MQ=255)

                                  ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                             >  2:2276336/1‑128 (MQ=255)

                                   tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                            <  1:7205256/128‑1 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                           >  2:4798816/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                           >  1:3013183/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                           >  2:7482847/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                           >  1:1010252/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATAATAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAATATTTAAATTAtttt                                                                                           >  2:2395723/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTATATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                           <  2:6492329/128‑1 (MQ=255)

                                     tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                          <  2:2143680/128‑1 (MQ=255)

                                       aaaTTATTTAATCATTTAAAAAAATAATTCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                        <  1:1336361/128‑1 (MQ=255)

                                            attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                   >  1:4157209/1‑128 (MQ=255)

                                             ttttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAtt                                                                                  >  2:1553508/1‑128 (MQ=255)

                                               ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCAATTTTTAAAAAAATTTATAATTTTAATAACTGAATTATATTTCAAATAGATAAATAATATTCAAATCAatat                                                                                >  2:1407248/1‑128 (MQ=255)

                                                 aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                              >  2:3561869/1‑128 (MQ=255)

                                                   cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                            <  1:6052934/128‑1 (MQ=255)

                                                      ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                         <  1:3777142/128‑1 (MQ=255)

                                                         aaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                      <  2:3164602/128‑1 (MQ=255)

                                                          aaaaaTAATACTTTTATTAGTTGATATTTTAGAATACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                     <  1:7791704/128‑1 (MQ=255)

                                                           aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑t                                                                   <  2:7596528/128‑1 (MQ=255)

                                                            aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑tt                                                                  <  1:3986749/128‑1 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTACCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTATTAATCAA‑ttt                                                                 >  2:5387029/1‑128 (MQ=255)

                                                              ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑tttt                                                                >  1:5105586/1‑128 (MQ=255)

                                                                   aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCt                                                           >  2:7712540/1‑128 (MQ=255)

                                                                    cTTTTATTATTTTATATATTATTAAACTTATAGCAATTTTTTAAAAAATTTTTAAATTTAATAAATGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAA‑TTTTATGCTc                                                          <  1:1676845/128‑1 (MQ=255)

                                                                    cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAATTTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTc                                                          >  1:7072896/1‑128 (MQ=255)

                                                                    cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTc                                                          >  1:7498622/1‑128 (MQ=255)

                                                                     ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑g                                                        >  2:4216709/1‑128 (MQ=255)

                                                                      tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑t                                                      >  2:6280772/1‑128 (MQ=255)

                                                                       ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTAAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑tt                                                     >  2:4084766/1‑128 (MQ=255)

                                                                                 atatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑tttttttttttt                                           >  2:6626427/1‑128 (MQ=255)

                                                                                 atatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑tttttttttttt                                           >  2:5520222/1‑128 (MQ=255)

                                                                                 atatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑tttttttttttt                                           >  1:6227816/1‑128 (MQ=255)

                                                                                  tatatTTTAAAAATAATATTATTTTTTTAAAAAATTTTTAATTTTAATAAATTATTTTTTTTTTAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAA‑TTTTATTCTC‑G‑ttttttttttttt                                          <  1:6365653/128‑1 (MQ=255)

                                                                                   atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATTATC‑G‑tttttttttttttt                                         >  1:1804283/1‑128 (MQ=255)

                                                                                   atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑tttttttttttttt                                         >  2:4372196/1‑128 (MQ=255)

                                                                                   atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑tttttttttttttt                                         >  1:5221599/1‑128 (MQ=255)

                                                                                       tAGAAAACTAATTTCAATATTTAAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTATTTTCAAATCAATATCTATAAATCAA‑ATTTATGCTC‑G‑TTTTTTTTTTTTTTTTTa                                     <  2:1904699/128‑1 (MQ=255)

                                                                                         gAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑T‑TTTTTTTTTTTTTTTTTACa                                   >  1:6943059/1‑128 (MQ=255)

                                                                                           aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑tttttttttttttttaaaaaat                                 >  2:6865449/1‑121 (MQ=255)

                                                                                           aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGATC‑G‑TTTTTTTTTTTTTTTTaaaaaa                                 >  1:906611/1‑128 (MQ=255)

                                                                                              ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑tttttttttttttttttttacaaaa                              >  1:6588896/1‑120 (MQ=255)

                                                                                              ataatattaattttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAATTTTTTAAATTTTTTTAAAATATTTTTTTTTTAATAAA‑TTTTTTGCTCTT‑TTTTTTTTTTTTTTTTTACaaaaa                               <  1:1553892/118‑1 (MQ=255)

                                                                                              aaactaGCACATTATTAAAAAAATTTTAATATTAAAAACTTAATTAATTTTTAAAATTTTAAATTATTTTAAAAAAATTATTTATTAATCAA‑TTTTTTGCTC‑GTTTTTTTTTTTTTTTTTTACaaaaa                               <  1:6836061/126‑1 (MQ=255)

                                                                                               ttataGAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTGTTAAATTTTTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTACAAAAata                             <  2:6131533/125‑1 (MQ=255)

                                                                                               tattaGCAATTTTTTAAATAATTTTTATTTTTTTTTACTGAATTTTTTTTTAAATTGTTTAATTATTTTCAAATCATTTTATTTTAATAAT‑TTTTTTTCTA‑G‑TTTTTTTTTTTTTTTTTACAAAAtta                             <  1:761869/125‑1 (MQ=255)

                                                                                                 ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTAAAAAAAataaa                           >  2:3052805/1‑126 (MQ=255)

                                                                                                 ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑‑TTTTTTTTTTTTTTTTA‑AAAAATATaaa                         >  2:6401358/1‑127 (MQ=255)

                                                                                                 ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑‑‑TTTTTTTTTTTTTTTTTAAAAAAATATaa                          >  2:17851/1‑128 (MQ=255)

                                                                                                  agaaatttttttAAAAAATTT‑TAATTTTAATAAATTTATTATTTTTTAAATTTTTAAATAATTTTAAAATAATTTTATATTAATAAA‑TTTTATTCTC‑GTTTTTTTTTTTTTTTTTTACAAAAATATaa                          <  2:6980997/122‑1 (MQ=255)

                                                                                                   ttcatttttttAAAAAATTTTTAATTTTAATAAATTTATTATTTTTAAATTTTTTAAATTATTTTAAAATCATTATATATTAATAAA‑TTTTATTCTT‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAat                         <  2:5049374/123‑1 (MQ=255)

                                                                                                   aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATATAat                         >  2:867066/1‑128 (MQ=255)

                                                                                                   aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑‑TTTTTTTTTTTTTTTTAAAAAAATATAatt                        >  2:2013288/1‑128 (MQ=255)

                                                                                                   aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑‑TTTTTTTTTTTTTTTTAAAAAAATATAatt                        >  2:7092685/1‑128 (MQ=255)

                                                                                                     taattttttAAAAAATTTTTAATTTTAATAAATTAAATATTTTTATAATTTTTTTATTTTTTTAAAATAAATATTTTTTAATAAA‑TTTTTTGCAG‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAattt                       <  2:3292214/125‑1 (MQ=255)

                                                                                                     cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAAAAAAatatttttt                       >  1:2700518/1‑123 (MQ=255)

                                                                                                     cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTCTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TATTATGCTC‑G‑TTTTTTTTTTTTTATTTAAAAAAATATAatta                       >  1:1953915/1‑127 (MQ=255)

                                                                                                      aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGATC‑G‑‑TTTTTTTTTTTTTTTTAAAAAAATATAttttat                     >  1:3357088/1‑128 (MQ=255)

                                                                                                      aCTTTTTTAAAAAATTTTTAATGTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTTTaaaaaaaataatat                      >  1:3172103/1‑119 (MQ=255)

                                                                                                        ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATATAatttatt                    >  1:6695255/1‑128 (MQ=255)

                                                                                                           tttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑GTTTTTTTTTTTTTTTTTTACAAAAAAATAatatatttt                  >  2:3667157/1‑127 (MQ=255)

                                                                                                            ttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTAAAAAAATAATAAtttattttat                >  2:7898600/1‑128 (MQ=255)

                                                                                                              aaaaaaTTTTTAATTTTAATAAATTAATTATTTTTTAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAA‑TTTTATTATA‑T‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTa              <  2:73319/128‑1 (MQ=255)

                                                                                                               aaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑GTTTTTTTTTTTTTTTTTTAAAAAAATAAAATTTATTttatta              >  2:7241305/1‑128 (MQ=255)

                                                                                                                  aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTAAACg          >  2:5984688/1‑128 (MQ=255)

                                                                                                                         aatttaaatAATTTAATATTTTTTTAAATT‑TTTAATTATTTTAAAATTAATATATTTTTATTAATTTTTTTGCTC‑‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTa  <  2:2936360/128‑1 (MQ=255)

||

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTC‑G‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420038‑420287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`