Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA98.6% 7076.8 / 66.2 2347intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1486/803);  minor base . (4/29);  total (1493/854)
Fisher's exact test for biased strand distribution p-value = 6.76e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2412 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349
                                                                                                                             |                                                                                                                                
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                >  1:6776054/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               >  2:4485050/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               <  1:201906/128‑1 (MQ=255)
  ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatt                                                                                                                              >  1:5223713/1‑128 (MQ=255)
   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                             >  1:4086658/1‑128 (MQ=255)
   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                             >  2:2424793/1‑128 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:967833/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                        >  2:4220190/1‑128 (MQ=255)
              ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                                  <  2:4355086/128‑1 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:4745214/1‑128 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCTCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                               >  2:1545395/1‑128 (MQ=255)
                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             <  1:2485891/128‑1 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                           >  1:2266569/1‑128 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                       >  2:884715/1‑128 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                       >  2:1150367/1‑128 (MQ=255)
                          ctaataTTTAGTATCATTAATTTATCGCCCTTGTATTAATTAGTAAGAAACTAAAACTTTTATCTTTTTAGATATTTTTTGTTTATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGcg                                                                                                      <  2:6446659/127‑2 (MQ=255)
                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt                                                                                                    >  1:3973715/1‑128 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAATAGA‑ttt                                                                                                   >  1:6205322/1‑128 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttt                                                                                                   >  2:171273/1‑128 (MQ=255)
                                 ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttttt                                                                                              >  2:5235872/1‑128 (MQ=255)
                                 ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttttt                                                                                              >  2:6814070/1‑128 (MQ=255)
                                  tAGTTTCATTTATTTATCGCACTTGTTTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttt                                                                                             >  1:6862249/1‑128 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑ttttttttt                                                                                             >  2:3524344/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttt                                                                                           >  2:2113447/1‑128 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttttttttt                                                                                          >  1:6432508/1‑128 (MQ=255)
                                       taatttatttATCGCAATTGTATGAATTAGTAAGAAAATAAAACTATTATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAAAAGC‑tttttttttttttt                                                                                        <  1:422561/126‑1 (MQ=255)
                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttattttttttttt                                                                                        >  2:3797270/1‑128 (MQ=255)
                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttt                                                                                       >  1:3634230/1‑128 (MQ=255)
                                        cATTAATTTATCGCACGTGTAGGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTAGATGCAAATTTTTAAAAAATTAAATAGAACTTGTAAACACTAGC‑ttttttttttttttt                                                                                       >  1:3843053/1‑128 (MQ=255)
                                         atttatttTTAGCAATTTTATTTATTATTTATAAAATAAAAATTTTTTATTTTTATATATTTTTTTTATATGCAAAATTTTTAAAAATTAATTTGAACTTGTAAAAAAAATC‑tttttttttttttttt                                                                                      <  2:594234/128‑1 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttttttttttttt                                                                                      >  1:2612143/1‑128 (MQ=255)
                                                 atagCACTTTTATTAATTTTTATTAAAATAAAAATTTTTTTTTTTTTTTTATTTTTTTTATATTAAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAA‑ATT‑TTTTTTTTTTTTTTTTTTTATAATg                                                                             <  2:2705312/125‑1 (MQ=255)
                                                 aTCGAAATTTTATTAATTATTTATAAAATAAAACTATTATATTTTTAGATATTTTTTGTATATTAAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATATC‑TTTTTTTTTTTTTTTTtataatgg                                                                              <  1:2912751/128‑5 (MQ=255)
                                                  tttcACTTTTTTTAATTATTAATAAAATAAAAAATTTATATTTTTTTTTTTTTTTTTTTTATTTAAATTTTTAAAAAATTTATTTTTAAATTTTAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATgg                                                                            <  2:2242778/125‑1 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttttttattatgta                                                                            >  2:292460/1‑120 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttttataatgtaaa                                                                            >  2:2539209/1‑118 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTtaatttaaattt                                                                        >  1:3727035/1‑118 (MQ=255)
                                                        ttttttGAATTTGTAAGAAACTAAAACTATTATATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                    <  1:3295706/123‑1 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGTAAata                                                                       >  2:5346907/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTATATAATGGAAttat                                                                      >  2:3952258/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAAtata                                                                     >  2:312747/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATaa                                                                    >  1:3166353/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAG‑‑ATTTTTTTTTTTTTTTTTTATAAtttaaaata                                                                      >  1:1876306/1‑120 (MQ=255)
                                                         tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTAATTATAATTTAAatat                                                                      >  1:1537653/1‑128 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑TTTTTTTTTTTTTTTTTTaatatgaaaaataaaa                                                                    >  1:4429832/1‑116 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAATAATGTAAATATaaa                                                                   >  1:4891835/1‑128 (MQ=255)
                                                            aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTATAAT‑ATTTAAATATaatt                                                                  >  2:41118/1‑126 (MQ=255)
                                                              taaTTAGTAATAAAAATAAAATAATTTATTTTTTTTTATTTTTTTTATATTAAAATTTTTAAAAAAATAATTTTTAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtttt                                                               <  1:4631450/127‑1 (MQ=255)
                                                                attttttataaaataaaaataTTTTTATTTTATATATTTTTTTTATTTTTAAATTTTTTAAAAATTTATTTTTAATTTTTAAAAATATC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtttt                                                               <  1:1143239/119‑1 (MQ=255)
                                                                 ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGTATATTTAAATTTTAgt                                                            >  2:2324709/1‑127 (MQ=255)
                                                                  tAGTAAGAAACTACAACTATTATGTTTTTAGATATTTTTTGGATATGCAAATTTTTAAAATAATAATTTGAACTTGTAAATAATAGC‑‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAATTTTTTTGTAt                                                         >  2:3667661/1‑128 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAATAATAGC‑‑‑TTTTTTTTTTTTTTTTTATATTTTAAATAAAAATTTTAgtt                                                           >  1:853534/1‑126 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGGAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TATTGGAAATATAAATTTTAgt                                                            >  1:5579625/1‑127 (MQ=255)
                                                                   attaataaACTAAAAATATAATCTTTTTAGATATTTTTTTTATATGGAAATTTTTAAAAAATTAATTTTTACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                           <  1:539458/126‑1 (MQ=255)
                                                                    ttattaaattaaaaataTTATTTTTTTTTTTAATTTTTTTTTATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAAGT‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                           <  1:5822045/123‑1 (MQ=255)
                                                                    ttaataaACTTAAATTATTATTATATTATATATTTTTTTTTTATTCATATTTTTAAAAAATTAATTTTTAATTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                         <  2:2007827/127‑1 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTTTAATTTAAAAATAAAattttttt                                                           >  2:4615774/1‑124 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAAtttaaatt                                                           >  1:6643427/1‑123 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATATTTTAAATAAAAATTTTAGTTa                                                          >  1:4532967/1‑128 (MQ=255)
                                                                        gAAAATTAAAATATTATATTTTTATAATATTTTTGTATATGCAAATTTTAAAAAAAATTATTTGAACATGTAAAAAA‑ATA‑CTTTTTTTTTTTTTTTTTTATATTGGAAATATAAATTTTAGGTAtttt                                                      <  2:5475505/128‑1 (MQ=255)
                                                                         taaaaaaaaataATATATTTTTATATTTTTTTTTTTTATTTTAATTTTTATAAAATTATTTTTTATTTTTAAAAAA‑ATT‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                     <  2:1877930/123‑1 (MQ=255)
                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTtttttg                                                  >  1:6525624/1‑128 (MQ=255)
                                                                            ctaaaactaTTAACTTATTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTAGAACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTATAAAATAAAAATAAAAATtatatttttagtatta                                                  >  2:673023/1‑116 (MQ=255)
                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTCTaa                                             >  1:51217/1‑128 (MQ=255)
                                                                                aattattatTTTTTTTTATATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  2:6181628/125‑1 (MQ=255)
                                                                                  ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTAATTTTTTTTTATaa                                             >  1:2384155/1‑128 (MQ=255)
                                                                                  atattttTTTTTTATTTATTTTTTTTTTTTTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑T‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                            <  1:3073187/127‑1 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTT‑‑TAATGAAAATATAAATTTTAGTTATTTTGTTGTATAATTa                                          >  2:3019929/1‑128 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTAT‑‑TGTAAATATAATTTTTTGTTATTTtgttgtgaatgtat                                         >  2:5129916/1‑120 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TAATGTAAATATAAATTTTATGTATTTTTTTGTTTattta                                          >  2:3426820/1‑124 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAAAATAAATTTTGTGTTTTTTGTTGTATAATTAt                                         >  2:6066784/1‑128 (MQ=255)
                                                                                         cTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTTTTTTTTTTTATAATTATATaa                                     >  2:3297456/1‑128 (MQ=255)
                                                                                         cTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                 <  1:5470583/115‑1 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTAATTTTTTTTTATAATTATATaaaa                                   >  1:1001558/1‑128 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAATTATAAATTTTAGTTTTTTTTTTGTGTATTTTTCTaaaaa                                  >  1:1345104/1‑128 (MQ=255)
                                                                                              tAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGCATTTTGTTGTCTAATTATCTaaaaaaa                                >  1:5976495/1‑128 (MQ=255)
                                                                                               ttatatTTTTTTTAATTTGAATTTTTTAAAAAATTAATTTGAAATTGTTAAAAA‑ATT‑GTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAAt                               <  2:753503/126‑1 (MQ=255)
                                                                                                ttttttttttttttATTCTAATTTTTAAAAAATTTTTTTTTAATTTTTAAAATTA‑T‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTACGTATCTAAAAAAATa                              <  1:6323900/124‑1 (MQ=255)
                                                                                                tttatTTTTTTTTTTTTAAAATTTTTTAAAAATTTATTTTTAATTTTAAAAAAT‑‑T‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  1:1763191/126‑1 (MQ=255)
                                                                                                 atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTTTAATGGAAATAAAAATTTTAGTTATTTTGTTGTATAAGTTTATAAAAAAATa                              >  2:5678559/1‑128 (MQ=255)
                                                                                                    ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATGTATTTTGTTTAATAATTTTCTAAAAAAATata                            >  1:1246101/1‑126 (MQ=255)
                                                                                                      ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAATTGTAAATAAAAATTTTAGTAATTTTTTTTTATAATTAAATAaaaaaaaaaaaata                        >  2:6619785/1‑126 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTGTATAATTATATAAAAAAATATaaataaaa                     >  1:5030760/1‑123 (MQ=255)
                                                                                                            ttatttaaaTTTTTAAAAAATTTATTTTTAATTTTTAAAAAT‑‑T‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  2:5033150/122‑1 (MQ=255)
                                                                                                            attttaaaaTTTTTAAAAAATTAATTTTAAATT‑TTAAAAATA‑T‑TTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:568866/122‑1 (MQ=255)
                                                                                                            atctaaataTTTTAAAA‑AATATATTTTTAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata                <  2:1310114/120‑1 (MQ=255)
                                                                                                             tttttaaaTTTTTAAAAAATTAATTTTAAATTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:6108030/123‑1 (MQ=255)
                                                                                                             tatTCAAATTTTTAAAAAATTAATTTTTACTTTTAAAAAAAATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:5321505/128‑1 (MQ=255)
                                                                                                             tatGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTAT‑ATTAAATTAAAAATTTTTGTTATTTTGTTGTATATTTATATAAAAAAATTTAAAGTAATAAat                 >  1:2128351/1‑128 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTAGGTATTTTTTTGTATAAGTTTATAAAAAAATATAAATTaaaaaaaa                >  1:3543678/1‑126 (MQ=255)
                                                                                                                 taaaTTTTTTAAAAAATATTTTGAACTTTTAAAAAATTTC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATAtt              <  1:3421078/127‑1 (MQ=255)
                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTAAAAAGTAAAAATAATTTTTATTTATTTTTTTGTAAAAATTTATAAAAAAAAAAAAAGTAAAAAAAAtt              >  2:5556998/1‑128 (MQ=255)
                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATTATGGAAATATAATTTTTATGTATTTTTTTTTCTATGTTTATAAAAAAATAAAAAGAAAAAAAttttt             >  1:4761738/1‑127 (MQ=255)
                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAATGTATTTTGTTGTCAAAGAATAAAAAAAAATATAAAGGAAAAAATAATGtt           >  2:1437180/1‑128 (MQ=255)
                                                                                                                   aaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTTTTTTTTTTTATAATTATATAAAAATATAGTAATGAAAAAGTAttaaat          >  1:1429520/1‑124 (MQ=255)
                                                                                                                    aTTTTTTAAAAATTAATTTTTAATTTTAAAAAA‑AAT‑TTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTTTTTTATAACTATAAAAAAAAATAGAAAGGAAAAAATATTGTTa          <  1:2750967/128‑1 (MQ=255)
                                                                                                                    aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAAAT‑AAAATAAAAATTTAATTTATTTTTTTTTAAAATAAAAAAAAAAAATAAAAATAAAAAAATAttaaaa          >  2:57990/1‑124 (MQ=255)
                                                                                                                        ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTGTGTAATTATATAAAAAATAAAAAAGCAAAAAAAATTTTTaaaa       >  2:6303707/3‑128 (MQ=255)
                                                                                                                         taaaaaaTTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTAAAAAAT‑TAAATAAAAATTTTATTTATTTTATTTAAAAATTAAATAAAAAATAAAAAAATAAAAAATATTTATaaaaat     >  2:4854657/2‑126 (MQ=255)
                                                                                                                         taaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAAATTAAATAATAAATATATTTTTTTTTGTTTTATAAGTATATAAAAAAAAAAAAAAGAAAATAAATTTTTaaattt     >  1:1658675/2‑125 (MQ=255)
                                                                                                                             |                                                                                                                                
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑