BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	20,023	2 bp→TA	intergenic (‑744/‑392)	JCVSYN2_00065 ← / → JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	20,023	0	A	T	99.8%	4968.0 / NA	1658	intergenic (‑744/‑393)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base A (2/1); new base T (887/766); total (891/767)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	20,024	0	T	A	99.8%	5027.7 / NA	1658	intergenic (‑745/‑392)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base T (3/0); new base A (888/767); total (891/767)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1687 total aligned reads displayed.

GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19901‑20145

||

gCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAAC‑AAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  2:3121621/128‑1 (MQ=255)

  aGGAAGAGCAAGATAAAAGGTAGTATTTGATGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTtata                                                                                                                     <  2:524165/128‑1 (MQ=255)

   ggAAGTGCAAGTTAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAatttatatatttatat                                                                                                                    <  2:2061661/128‑1 (MQ=255)

    gAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGATTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTATATACTTTCTATTTTTAATTTAtatatttatatt                                                                                                                   >  2:665908/1‑128 (MQ=255)

     aaGAGCAAGATAAAAGGTAGTATTAGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTa                                                                                                                  <  1:3229692/128‑1 (MQ=255)

             gATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTTACTTTCTATTTTTAatttatatatttatat                                                                                                                    <  1:1000178/119‑1 (MQ=255)

             gATAAAAGGTAGTAATTGTTGGCGATCCCCCTAGAGTCTTTTTCATCTTCGGAAAACAAAAACTATTATAACTTTAATATC‑TTTATATACTTTCTTTTTTTAATTTATATATTAATATTAAAAAAttt                                                                                                          >  2:2322310/1‑128 (MQ=255)

              aTAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTa                                                                                                         <  2:2466478/128‑1 (MQ=255)

               tAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                        <  2:770444/128‑1 (MQ=255)

               tAAAAGGTAGTATTTGTTGGCGATCAAACTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                        <  1:3892659/128‑1 (MQ=255)

                aaaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       >  2:2680705/1‑128 (MQ=255)

                 aaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                      <  2:1279193/128‑1 (MQ=255)

                  aaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                     >  1:1505233/1‑128 (MQ=255)

                  aaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                     <  1:1880863/128‑1 (MQ=255)

                   aGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatta                                                                                                    <  1:3795878/128‑1 (MQ=255)

                       agtagtTGTTTGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataat                                                                                                <  2:3411134/128‑1 (MQ=255)

                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               <  2:3048008/128‑1 (MQ=255)

                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               <  2:1389521/128‑1 (MQ=255)

                        gtaTATGATGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               <  2:32191/128‑1 (MQ=255)

                         taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                              >  2:1827810/1‑128 (MQ=255)

                         taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                              <  2:2805439/128‑1 (MQ=255)

                           tttGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                            <  1:291436/128‑1 (MQ=255)

                            ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttt                                                                                           >  1:259984/1‑128 (MQ=255)

                            ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttt                                                                                           >  1:679242/1‑128 (MQ=255)

                               ttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTta                                                                                        <  1:764901/128‑1 (MQ=255)

                                tgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTtat                                                                                       >  1:1064538/1‑128 (MQ=255)

                                    gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                   <  2:2733638/128‑1 (MQ=255)

                                      tCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACg                                                                                 >  1:3149684/1‑128 (MQ=255)

                                       cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                >  1:1275697/1‑128 (MQ=255)

                                       cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                <  2:1008867/128‑1 (MQ=255)

                                       cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTAGTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCActt                                                                                >  2:3914127/1‑126 (MQ=255)

                                        gcacTAGAGTCTTTTACATCTTCGTAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTAAATATATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  2:2550043/125‑1 (MQ=255)

                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  2:44430/128‑1 (MQ=255)

                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  2:1910674/128‑1 (MQ=255)

                                        ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  2:3571010/128‑1 (MQ=255)

                                         cccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGa                                                                              >  2:1971391/1‑128 (MQ=255)

                                          ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             <  2:144805/128‑1 (MQ=255)

                                           cTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATa                                                                            >  2:1131662/1‑128 (MQ=255)

                                            tAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                              >  2:2884100/1‑109 (MQ=255)

                                             agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                          <  1:1561406/128‑1 (MQ=255)

                                              gagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAtt                                                                         <  1:2497256/128‑1 (MQ=255)

                                               agTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTa                                                                        >  1:1361590/1‑128 (MQ=255)

                                               agTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCATTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTa                                                                        <  2:1630951/128‑1 (MQ=255)

                                                gTCTTTTACATCTTCGGAAAACAAATACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                       <  2:3459752/128‑1 (MQ=255)

                                                gTCTTTTACATCTTCGGAAAACAAAAACTTTTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTTTATTAAAAAATTTAATTTATAATTATTTTTATAGCCCGTGATAATTaa                                                                       >  1:2096820/1‑128 (MQ=255)

                                                gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                       >  1:2388478/1‑128 (MQ=255)

                                                gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                       >  2:1227175/1‑128 (MQ=255)

                                                  cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                     <  1:909687/128‑1 (MQ=255)

                                                  cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                     <  2:2196882/128‑1 (MQ=255)

                                                   ttttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATATCACGTGATAATTAAAAg                                                                    >  1:2709245/1‑128 (MQ=255)

                                                    tttACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGt                                                                   <  2:1572304/128‑1 (MQ=255)

                                                      tACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTa                                                                 >  2:3265581/1‑128 (MQ=255)

                                                       aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATATAACTTGATAATTAAAAGTTAg                                                                >  2:1074170/1‑128 (MQ=255)

                                                       aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                >  2:3159675/1‑128 (MQ=255)

                                                         aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTta                                                                                                                       >  1:161005/1‑71 (MQ=255)

                                                         aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATATATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTg                                                              >  1:1944877/1‑128 (MQ=255)

                                                         aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTATACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTg                                                              <  2:2399768/128‑1 (MQ=255)

                                                          tattaggAAAAAAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTTATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                             <  2:3775184/123‑1 (MQ=255)

                                                             tCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACa                                                          <  2:1328801/128‑1 (MQ=255)

                                                               ggAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAAc                                                           >  1:1992927/1‑125 (MQ=255)

                                                                 aaaaCAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                      <  2:2244926/128‑1 (MQ=255)

                                                                  aaaCAAAAACTATATTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaa                                                     <  1:2844484/128‑1 (MQ=255)

                                                                      aaaaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       <  1:810711/74‑1 (MQ=255)

                                                                       aaaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       >  1:2773692/1‑73 (MQ=255)

                                                                        aaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATATTTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAATCAGTg                                               >  1:41040/1‑128 (MQ=255)

                                                                        aaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               <  2:3976810/128‑1 (MQ=255)

                                                                          aCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGAAAATTAAAAGTTAGTGAACAAGAAAACAgataa                                             >  2:3719711/1‑124 (MQ=255)

                                                                          aCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGaa                                             >  1:3543230/1‑128 (MQ=255)

                                                                           cTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            >  1:1452860/1‑128 (MQ=255)

                                                                              ttttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:1769148/1‑128 (MQ=255)

                                                                              ttttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:587934/1‑128 (MQ=255)

                                                                              ttttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  1:1709540/1‑128 (MQ=255)

                                                                              ttttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:2930516/1‑128 (MQ=255)

                                                                              ttttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAAAAAGAAAACAGTGAAGCAc                                         >  2:894222/1‑128 (MQ=255)

                                                                              ttttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGAGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:2148060/1‑128 (MQ=255)

                                                                               tttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAcc                                        >  1:2168478/1‑128 (MQ=255)

                                                                                ttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCa                                       <  1:1782769/128‑1 (MQ=255)

                                                                                    cTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGa                                                       <  2:722515/108‑1 (MQ=255)

                                                                                    cTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   <  1:3637516/128‑1 (MQ=255)

                                                                                    cTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   >  2:3652875/1‑128 (MQ=255)

                                                                                       tAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTg                                >  1:330849/1‑128 (MQ=255)

                                                                                          tttC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAc                             >  1:3468856/1‑128 (MQ=255)

                                                                                          tttC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGAACCAGTTTCTGAAc                             >  1:532334/1‑128 (MQ=255)

                                                                                           ttC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAcc                            <  1:3981505/128‑1 (MQ=255)

                                                                                            tC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCa                           >  2:1503781/1‑128 (MQ=255)

                                                                                               tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTa                                                                        <  1:3410749/81‑1 (MQ=255)

                                                                                                    ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                         >  2:1689446/1‑123 (MQ=255)

                                                                                                     tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAagaaga                   >  2:3835400/1‑128 (MQ=255)

                                                                                                      aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGAc                  >  2:262050/1‑128 (MQ=255)

                                                                                                       cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                 <  1:3067141/128‑1 (MQ=255)

                                                                                                        tttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAACAGTTTCTGAACCAAAAGAAGACGa                >  1:1403640/1‑128 (MQ=255)

                                                                                                         ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa               >  1:60753/1‑128 (MQ=255)

                                                                                                          tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAATAAGACGaaa              >  2:841375/1‑128 (MQ=255)

                                                                                                          tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa              >  2:3350590/1‑128 (MQ=255)

                                                                                                          tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACAAAAAGAAGACGaaa              >  1:2258160/1‑128 (MQ=255)

                                                                                                            tATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaa            >  1:3687055/1‑128 (MQ=255)

                                                                                                                 ttAATTTATATATTTATAATAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAATTTAGTGAACAAGAAAACACTGAAGCACCAGTTTCTGACCCAAAAGAAGACGAAAAAACaaa       >  2:40121/1‑128 (MQ=255)

                                                                                                                  tAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAACAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaa      <  2:3495653/128‑1 (MQ=255)

                                                                                                                    atttatatatttatatTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAATCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaaa    >  1:2980192/1‑128 (MQ=255)

                                                                                                                      ttATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAg  <  1:1161735/128‑1 (MQ=255)

||

GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19901‑20145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`