Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,435,727 | 0 | G | A | 43.5% | 92.4 / 27.4 | 62 | intergenic (‑108/+33) | uspF/ompN | stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
Reads supporting (aligned to +/- strand): ref base G (31/4); new base A (0/27); total (31/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.25e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTG > NC_000913/1435579‑1435871 | gAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa < 2:643113/151‑1 (MQ=255) cGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAg < 1:482222/151‑1 (MQ=255) ggAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGc > 2:242050/1‑151 (MQ=255) gAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGcc > 2:337502/1‑151 (MQ=255) aaaTATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGccc < 1:450072/151‑1 (MQ=255) atCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGcc > 2:134502/1‑150 (MQ=255) atCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGcc > 1:510255/1‑150 (MQ=255) tAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAAc > 1:825160/1‑150 (MQ=255) ggACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCTATAACAGCg < 1:639122/151‑1 (MQ=255) aGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGc > 2:586923/1‑149 (MQ=255) ttCTGTTAAAAAAACCTCCTTTTTTAGTATCCGAAAAAAGTGTAACGCAAGATGACATTTTTTGTGTAAGGAGGGATTTCACATTTTTAATTTAGATAAAAGGAGGAAAAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCaggag < 2:392081/147‑1 (MQ=255) ttaaTAAACCCCCCTTTTTTATTATCCGAAAAAAGTGAAACGCAAGATGACTTTTTTTGTTAAATGAGGGAGTTCACTTTTTTAATTTAGATAAAAGGAGAAAGAATAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGAttt < 2:793359/144‑1 (MQ=255) aTAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTg > 2:743800/1‑151 (MQ=255) tAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTg > 2:338216/1‑150 (MQ=255) aaaaCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGAt > 2:49489/1‑151 (MQ=255) aaCCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATa > 1:55737/1‑150 (MQ=255) cctTTTTTAGTACCCGCATAAAGTGTACCGCCAGATGACATTTTTTGTGTAAGGACGGAGTTCACATTTTAAATTTAGATAAAGGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCa < 2:313306/150‑1 (MQ=255) ttttttAGTACCCGCAAAAAGTGTACCCCCAAATAACATTTTTTGTGTAATGACGGATTTCACATTTTTAATTTAGATAAAGGGAGAAAAAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGa < 2:190369/148‑1 (MQ=255) tGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAc > 1:306551/1‑151 (MQ=255) tGTTTTAGTATCCGAATAAAGTGTAACGCCAGATAACTCTTTTTGTGAAAAGACGGAGTTCACATTTTTAATTTAAATAAAAGGAGGAAAAAAAAGAAAAAAAGGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATaaa < 1:451562/145‑1 (MQ=255) atttcccGCATAAAGTGAAACGCAAGATGACATTTTTTGTGTAATGACGGAGTTCACTTTTTTAATTTAGATCAAGGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTaaa < 1:774321/145‑1 (MQ=255) gTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc > 2:639038/1‑151 (MQ=255) tATCCGAATAAAGTGTAACGCCAGAGGACTTTTTTTGTGTAATGAGGGAGTCCACATTTTTATTTTAGATAAAAGGAGGAAGAATAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc < 2:744023/150‑1 (MQ=255) tATCCGAAAAAGGTGTACCGCAAGATGACTTTTTTTGTGTAATGACGGATTTCACTTTTTTAATTTAGATAAAAGGAGAAAGAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc < 2:475333/150‑1 (MQ=255) aTCCGCATAAAGTGTAACGACAGATGACTTTTTTTGTGAAAGGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc < 1:140622/149‑1 (MQ=255) gaaaaaaGTGTAACGCCAGATGACTTTTTTTGTGTAATGACGGAGTTCACTTTTTTAATTAAGAAAAAAGGAGAAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCg < 1:55061/142‑1 (MQ=255) aTAAAGTGTACCCCCAGATGACTTTTTTTGTGTAATGCCGAAGTTCAAATTTTTAATTAAGAACAAAGGAGAAAAAATAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAatat < 2:679313/151‑1 (MQ=255) aaaGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatc > 1:653634/1‑150 (MQ=255) gtgtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCATAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCg > 1:514604/1‑151 (MQ=255) gtgtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCg > 2:501484/1‑151 (MQ=255) gtAACGCCAGATGACATTTTTTGTGTAATGACGGAGTTCACATTTTTAATTAAGATAAAGGGAGGAAGAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCgg < 2:926912/150‑1 (MQ=255) aaCGCGAGATGACTTTTTTTGTGAAATGACGGATTTCACATTTTAAATTAAGATCAAGGGAGAAAAAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTa < 1:683777/150‑1 (MQ=255) aaCGCCAGATGACTTTTTTTGTGTAATGACGGATTTCACTTTTTTAATTAAGATAAAGGGAGAAAAAAAAAAAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTag < 1:244407/151‑1 (MQ=255) aCGCCAGATGACATTTTTTGTGTAGTGACGGAGTTCACTTTTTTAATTTAGATCAAAGGGGAAAAAAAAGGCAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatcat < 1:686073/144‑1 (MQ=255) aCGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGt > 2:256070/1‑148 (MQ=255) cacTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTgg > 1:283069/1‑151 (MQ=255) cTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGc > 2:781937/1‑150 (MQ=255) ttttGTGTAATGACGGATTTCACATTTTTAATTTAGATAAAGGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAg < 2:896678/150‑1 (MQ=255) ttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGc > 1:291161/1‑149 (MQ=255) ttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGt > 2:695672/1‑151 (MQ=255) gtatAATGACGGAGTTAACTTTTTTAATTAAAATCAAAGGAGGAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGt < 1:927851/146‑1 (MQ=255) aaTGAGGGATTTCAATTTTTTAATTTAAACAAAGGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGc < 2:121835/151‑1 (MQ=255) aaTGACGGAGTTCATTTTTTTAATTAAGAACAAAGGAGAAAAAATAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGaa < 1:446583/149‑1 (MQ=255) aaTGACGGAGTTCACATTTTTAATTTAGATAAAAGGAGAAAGAATAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAg < 2:910172/150‑1 (MQ=255) aaTGACGGAGTTCACATTTTTAATTTAGACCAAAGGAGGAAGAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTa < 2:420047/146‑1 (MQ=255) aaTGACGGAGTTCACATTTTTAATTAAGAGAAAGGGAGAAAAAAAAAGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTa < 2:447665/146‑1 (MQ=255) atgaggggtttaaaaTTTTAAATTAAAAAAAAGGGAGGAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTa < 2:460852/131‑1 (MQ=255) gACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTg > 1:42572/1‑150 (MQ=255) gACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGt > 1:712267/1‑151 (MQ=255) cGGAGTTCACATTTTTAATTAAGAACAAAGGAGGAAAAAAAAAAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGaa < 1:615965/144‑1 (MQ=255) ggATTTCACATTTTTAATTAAGAAAAAGGGAGGAAAAATAAAAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCt < 1:557904/146‑1 (MQ=255) cacaTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCAt > 2:109432/1‑151 (MQ=255) aaTTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGt > 2:765079/1‑151 (MQ=255) ttaagaaaaaaGGAGAAAAAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGtt < 1:311344/142‑1 (MQ=255) ggaggaAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCTACAGGTTGATTTTATAGTc > 2:603633/1‑151 (MQ=255) ggagaaAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAg < 1:907964/149‑1 (MQ=255) gaggaAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTc > 1:471496/1‑150 (MQ=255) gaggaAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTc > 1:730370/1‑150 (MQ=255) gaagaaTAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACg > 2:800196/1‑151 (MQ=255) agaaTAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGt > 1:574362/1‑150 (MQ=255) tAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTg > 2:555558/1‑151 (MQ=255) tAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTg > 1:874477/1‑151 (MQ=255) | GAATCGGAAATATCGATAGGGACAAGAATCGTTCTGTTCATAAAACCTCCTGTTTTAGTATCCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCTTCATCCAACAGGTTGATTTTATAGTCAACGTAGGTG > NC_000913/1435579‑1435871 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |