Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,646	0	T	G	45.8%	71.0 / 10.7	49	V316G (GTA→GGA)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base T (6/20);  new base G (22/0);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAAT  >  NC_000913/2252496‑2252792                                                                                                                                                       |                                                                                                                                                      atCAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGt                                                                                                                                                       <  1:912860/151‑1 (MQ=255)     gCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGtgt                                                                                                                                                     >  1:863415/1‑147 (MQ=255)       aaGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGgat                                                                                                                                                     >  1:405260/1‑147 (MQ=255)               cAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCtt                                                                                                                                         <  2:844809/150‑1 (MQ=255)                aGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATTG‑CTTTCtt                                                                                                                                         >  2:671442/1‑149 (MQ=255)                  cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAg                                                                                                                                     <  1:788468/151‑1 (MQ=255)                  cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGG‑GCTGACGATGGTTTTATGGCGGGGCCGGGATATAG‑Gtttttttta                                                                                                                                      >  2:461679/1‑146 (MQ=255)                     cTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAATGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTCGCAGAAAG‑Ctttttt                                                                                                                                         >  1:219138/1‑144 (MQ=255)                       gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATa                                                                                                                                 <  2:175922/150‑1 (MQ=255)                       gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAG‑Tttttttttagg                                                                                                                                    >  1:435511/1‑142 (MQ=255)                           gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAG‑CTTTTTTTTAGGGTACAGtt                                                                                                                            >  2:153704/1‑151 (MQ=255)                            tttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGtt                                                                                                                            <  2:238093/150‑1 (MQ=255)                               aTTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGTGTATAG‑CTTTTTTTATGGAAACAGatt                                                                                                                           >  1:553491/1‑148 (MQ=255)                                   gCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGAc                                                                                                                    <  1:882942/151‑1 (MQ=255)                                     ccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAG‑CTTTTTTGAAGGATACAGTTTTCGCGAc                                                                                                                    >  1:100111/1‑149 (MQ=255)                                           aGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAG‑CTTTTTTGTAGGATAAAGTTTTCCCGGCAGCGcc                                                                                                              >  1:236460/1‑149 (MQ=255)                                               aGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGtttt                                                                                                        <  1:609041/151‑1 (MQ=255)                                                gTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATTA‑CTTTCTTTTAGGATAAAGTTTTCCCGGCAGCCCCCCtttt                                                                                                        >  2:118510/1‑150 (MQ=255)                                                   tATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCg                                                                                                     <  2:745601/150‑1 (MQ=255)                                                   tATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAG‑CTTTTTTTTAGGAGAAAGTTTCCGCGGCAGCGCCCGCTTTGTgg                                                                                                    >  2:127007/1‑151 (MQ=255)                                                               aaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑ATTTCTTGTAGGATAAAGTTTTCGCGACAGCGCCCGCTTTTCGGTAGCCTGCGC‑gg                                                                                       >  2:550688/1‑151 (MQ=255)                                                                 ggATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGTATATAG‑CTTTCTTTAAGGATACAGTTTCCCCGACAACGCCCCTTTTGCGGTAGCCTGCGC‑gg                                                                                       >  1:198631/1‑149 (MQ=255)                                                                   aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAG‑GTTTCTTTTAGGATAAAGTTTTCGCGACAGCGCCCGTTTTGCGGGAGCCTGCGC‑GGCaa                                                                                    >  2:173444/1‑150 (MQ=255)                                                                     ggAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑tt                                                                                 <  2:678257/150‑1 (MQ=255)                                                                         aaCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCa                                                                                     <  2:721204/143‑1 (MQ=255)                                                                         aaCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAA‑CTTTCTTTTAGGATACAGTTTTCGCGACAGCGCCCCTTTTTCGGTAGCCTGCGC‑GGCAA‑ttt                                                                                >  2:558488/1‑147 (MQ=255)                                                                           cAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAG‑CTTTCTTTTAGGATACAGTTTTCGCGGCCGCGCCCGTTTTTTGGGGGCCTGCGC‑GGGCA‑ttt                                                                                >  2:145895/1‑145 (MQ=255)                                                                               ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAT‑CTTTCTTTTAGGGTACAGTTTTCGCGGCCGCCCCCCGTTTTTGGGAGCCTGCGC‑GGCCA‑ttttccccccc                                                                        >  1:69964/1‑141 (MQ=255)                                                                                 ttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATTG‑GTTTTTTTAAGGATACAGTTTTCGCGACAGCGCCCGTTTTTCGGTAGCCTGCGC‑GGCCA‑TTTTGCGCGcc                                                                        >  2:329438/1‑147 (MQ=255)                                                                                 ttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAA‑CTTTTTTTTAGGATACAGTTTTCGCGACAGCGCCCCGTTTTCGGGAGGCTGCGCGGGCAATTTTcgcgcc                                                                          >  2:843625/1‑146 (MQ=255)                                                                                     tGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCCGGATATAGACTTTTTTG‑AGGGTAAAGGTTTCGCGGCAGCGCCCCCTTTTTTGGAGCCTGCGC‑GGCCA‑TTTTTCCCGCCcgcg                                                                    >  1:753637/1‑147 (MQ=255)                                                                                      gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCa                                                                 <  1:131836/149‑1 (MQ=255)                                                                                      gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAg                                                                <  1:857733/150‑1 (MQ=255)                                                                                        ccGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAG‑GTTTTTTTTAGGAGAAAAATTTCCCGCCAGCGCCCCGTTTTTGGTGGCCCGCGC‑GGCAA‑ATTCTCGCGCCCGc                                                                     >  2:662165/1‑143 (MQ=255)                                                                                         cgcgCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAG‑CTTTTTTTAAGGATACAGTTTTCGCGACAGCGCCCCCTTTTCGGTAGCCTGCGC‑GGCCA‑TTTTGCGCGCCCGCgg                                                                   >  2:608019/1‑144 (MQ=255)                                                                                            gcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAG‑GTTTTTTTTAGGATAAAGTTTTTGCGGAAAGGGCCGGTTTGGGGTAGGCTGGGC‑GGGAA‑ATTTGCGCGGCAGGGGGAgggt                                                             >  2:85808/1‑144 (MQ=255)                                                                                                          cAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCGGGGATATAG‑TTTTTTTTTAGGAAACAGTTTTCGCGGGAGCGCCCGTTTTTTGGTAGCCTGCGC‑GGCGA‑ATTTGCGCGCCACCGGCAGCTTAAAAAaccccaccccctc                                           >  1:97695/1‑147 (MQ=255)                                                                                                            gTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTg                                         <  1:380487/151‑1 (MQ=255)                                                                                                                aCAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAA‑CTTTTTTTGAGGATACAGTTTTCGCGGCAGCGCCCCCTTTTTGGGAGCCTGCGC‑GGCAA‑TTTTGCGCGCCCGCGGCCGCTTTAAAAAACCCACCCTGTCTGcc                                       >  1:86996/1‑149 (MQ=255)                                                                                                                 cAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGAt                                    <  2:405308/151‑1 (MQ=255)                                                                                                                  aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAA‑ATTTTTTTTAGGATAAAGTTTTCCCGACAGCGGCCCTTTTTTGGGAGCCTGCGC‑GGCCA‑ATTTGCGCGCCCGCGGCAGCTTTAAAAAACCCACCCCCTCTGCCggt                                    >  2:723831/1‑148 (MQ=255)                                                                                                                   gTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATaa                                  <  1:499603/151‑1 (MQ=255)                                                                                                                     tttGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAAc                                 <  2:321984/150‑1 (MQ=255)                                                                                                                     tttGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAAc                                 <  1:134891/150‑1 (MQ=255)                                                                                                                           gCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTGCCCTGTCTGCCGATAACGCGCtt                           <  1:890681/150‑1 (MQ=255)                                                                                                                             tGACGATGGTTTTATGGCGGGCCCGGGGATTAG‑CCTTCTTTGATGATATAGTTTTTGTGGCAGCGCCCGTCTTTCGGTTGGCTGCGC‑CGCAA‑TTTTACGCGCCCGCGGGCGCCTAAAAAAACCTCCCCCGCCTCCCggtgaaccgcc                            >  1:732644/1‑137 (MQ=255)                                                                                                                              gACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTc                         <  2:598574/149‑1 (MQ=255)                                                                                                                                          aTGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATgg           <  2:281620/151‑1 (MQ=255)                                                                                                                                          aTGGCGGGCCCGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGGAGCCTGCGC‑GGCAA‑TTTCGCGCGCCCGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATgg           >  1:705621/1‑151 (MQ=255)                                                                                                                                              cGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa       <  2:583215/151‑1 (MQ=255)                                                                                                                                                   cTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAAt  >  2:118576/1‑151 (MQ=255)                                                                                                                                                   cTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAAt  >  1:528055/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                      ATCAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAG‑CTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAA‑TTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAAT  >  NC_000913/2252496‑2252792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑