Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,265,639	0	A	C	42.6%	96.6 / 10.0	55	T64P (ACC→CCC)	yeiP	elongation factor P‑like protein
Reads supporting (aligned to +/- strand):  ref base A (26/5);  new base C (0/23);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 9.29e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCC  >  NC_000913/2265493‑2265772                                                                                                                                                    |                                                                                                                                       aCAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑g                                                                                                                                    <  1:929597/150‑1 (MQ=255) aCAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑g                                                                                                                                    <  1:650509/150‑1 (MQ=255) aCAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑Gt                                                                                                                                   >  2:491143/1‑151 (MQ=255)     cGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACg                                                                                                                               >  1:629798/1‑151 (MQ=255)              gctgctgGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGAccc                                                                                                                        <  2:491015/149‑1 (MQ=255)              gctgctgGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACccg                                                                                                                       <  1:56408/150‑1 (MQ=255)               ctgctgGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACccgcc                                                                                                                     >  2:914218/1‑151 (MQ=255)                  ctgGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGtt                                                                                                                  >  1:539421/1‑151 (MQ=255)                   tgtttaggaatattgatattCAGCCGCCCACGGCCCGCGGTGCCGCTACGCTGTAAAAAATGCTTTTTTCTGTGGTCCGTCCCGGGCTG‑AAAGTAGAAGAGCTTTCCAAAGGTGATGATATCGTTGACCCC‑GGGACGCTGACCCGCCGt                                                                                                                   <  1:55365/140‑1 (MQ=255)                           gatattgatattCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACt                                                                                                         >  2:430461/1‑151 (MQ=255)                                  atattCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCt                                                                                                   >  1:182961/1‑150 (MQ=255)                                        agcccccccCTCCCGGCGGGCCCGCTACGCTGTAAAAATTGGTTTTTTCTGATCTCCTTCCCGGGCTA‑AAAGTAAAAGCGCTTTAAAAGGGTGTTGTTATTGTTGCCCCG‑GTGACCCTACCCCGCCGTTACGTTGACTTCTCCTATGTCGa                                                                                            <  1:3343/146‑1 (MQ=255)                                           ccccccccccccGCGGTGCCGCTACGTTGAAAAAAATGCGTTTTTCTGATGTCCGTCCGGGGCTA‑AAAGTAGAAGGGTGTTTAAAGGGTGATGATTTCTTTGACCCC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGAt                                                                                           <  2:379171/140‑1 (MQ=255)                                           cGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATgg                                                                                         >  1:243261/1‑151 (MQ=255)                                           cGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATgg                                                                                         >  2:377236/1‑151 (MQ=255)                                             ccccccccccGCGGGGCCGATACGGTGTAAAAATGGTTTTTTTCTGATCTCCGTCCCGGGCAA‑AAAGAAGAAGGGCGTATAAAGGGTGGTAATACCGTTAACCCC‑GGGCCCCGGCCCCGCCGTTACGTTGACTTCTCCTATGTCGAt                                                                                           <  1:900572/140‑1 (MQ=255)                                                 cTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACg                                                                                    >  2:122064/1‑150 (MQ=255)                                                    cccGGGGTCCCGCTACGCTGTAAAAAATGTTTTTTTTTTATGCCCTTCCCGGTCTA‑AAAGTAGAAGAGCTTTTAAAAGTTGTTAATTTCTTTGACCCG‑GTGCCCCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAat                                                                                 <  1:805554/150‑1 (MQ=255)                                                    cccGCGGTGCCGCTAGGTTGTAAAAAATGCTTTTTTCTGATGCCCGT‑CCGGGGTGAAAAGTAGAAGAGGTTTTAAAAGGTGATGATTTCTTGGACACC‑GTGCCGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACg                                                                                    <  2:525197/147‑1 (MQ=255)                                                       gggggcccGCTACGCTGTAAAAAATGTTTTTTTCTGATGCCCGTCCCGGGCTA‑AAAGTAGAAGAGCTTTTAAAGGGTAATGATTTCTTTGACCCC‑GTGCCGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAatat                                                                               <  2:325377/143‑1 (MQ=255)                                                       gcgGGGCCGTTAGGCTGTAAAAAATGTTTTTTTCTGATGTCCGTCCGGGGTTG‑AAAGTAAAAGAGTTTTTAAAGGGTGATGTTTTCTTTGCCCCG‑GTGCCGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATg                                                                              <  1:75046/150‑1 (MQ=255)                                                        cgGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTc                                                                            >  1:640133/1‑151 (MQ=255)                                                         ggTGCCGCTACTCTGAAAAAATTGTTTTTTTCTGATGCCCGCCCGGGGCTA‑AAAGTAGAAGAGCTTTTAAAGGGGGATAATTTCG‑TGACCCCGGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATg                                                                              <  1:914109/148‑1 (MQ=255)                                                               gCTACGCTTTAAAAAATGCTTTTTTCTGATGTCCGTCCGGGGGAG‑AAAGTAAAAGAGCGTTTAAAGGGTGATTATAGCGTTGCCCCG‑GTGCCGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTAt                                                                       <  2:310643/149‑1 (MQ=255)                                                               gCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATgg                                                                     >  2:466560/1‑151 (MQ=255)                                                                 tACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGAt                                                                   >  1:442863/1‑151 (MQ=255)                                                                      tttaaaatagtcttttttGTGATGGCCGTCCCGGTCAG‑AAAGGAGGGTATCTTTCCAAAGGTAATGATACTGTTGCCCCC‑GAGGCCGTAACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGAt                                                                   <  1:844432/133‑1 (MQ=255)                                                                      tataaaaaaTGTTTTTTTTGGTTGTCCGTCCCGGGCTA‑AAAGAAGAAGAGCGTTTCAAAGGTGATAATATCGTTGACCCC‑GTTCCGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaag                                                               <  1:847255/145‑1 (MQ=255)                                                                        tACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaaga                                                              >  2:590356/1‑149 (MQ=255)                                                                        caCAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAACCTTCCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACACGCCGTTACGTTGACTTCTCCTATGCCGATGGCAACGGATATGTCTTTATGGATAaagacg                                                            >  1:518308/2‑149 (MQ=255)                                                                        aaaaaaaTGTTTTTTTGTGATGCCCGTCCGGGGCTA‑AAAGTAAAAGAGCTTTAAAAGGGTGATGATTTCTTTAACCCG‑GGGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaagaag                                                            <  1:935989/148‑1 (MQ=255)                                                                         aaaaagtgtttttttCTGATGGCCGTGCGGGGGAA‑AAAGGAGAAGAGCTTTAAAAAGGTTTTAATATCGTTGACGCG‑GTGCCGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAagaaga                                                           <  1:25770/141‑1 (MQ=255)                                                                         aaaaaaTGTGTTTTTCTGTGGCCCGTCCGGGGATA‑AAAGAAAAAGAGCGTTAAAAGGGTGATGATATTTTTGACCCG‑GTGCCGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaagaa                                                             <  1:756304/147‑1 (MQ=255)                                                                         aaaaaaTGCGTTTTTCTGGTGTCCGTCCCGGGCAA‑AAAGAAGAGGAGCGTTTAAAAGGTGATAATATCTTTGACCCG‑GTGACGCGGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaag                                                               <  2:857315/145‑1 (MQ=255)                                                                               tGCTTTTTTGTGATGTCCGTCCCGGGAAA‑AAAGAAGAAGGGTTTTAAAAAGGTAATAATTTCTTTAACCCG‑GTGCCGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACtat                                                       <  1:466735/149‑1 (MQ=255)                                                                                gtttttttCTGATCTCCGTCCCGGGCAG‑AAAGTAGAAGAGCGTTTAAAAGGGTATGTTATCGTTGACCCC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACtata                                                      <  1:673127/146‑1 (MQ=255)                                                                                                cGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCAcc                                     >  2:103658/1‑150 (MQ=255)                                                                                                    ccGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCaa                                   >  1:774110/1‑148 (MQ=255)                                                                                                     cGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCaa                                   >  1:674391/1‑147 (MQ=255)                                                                                                     cGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCaa                                   >  1:620512/1‑147 (MQ=255)                                                                                                     cGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCaa                                   >  2:759858/1‑147 (MQ=255)                                                                                                       ggCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATc                              >  1:270800/1‑150 (MQ=255)                                                                                                              aagaagaagGGTGTTTCAAGGGTAATGATATCTTGCCCCCC‑GGGCCGCGGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTga                       <  2:89452/151‑1 (MQ=255)                                                                                                               aGTAGAGGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTga                       >  2:534809/1‑150 (MQ=255)                                                                                                                  agaagaGCTTTTAAAAGGTGATGATATCGTTGCCCCC‑GTGACGCTGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTgaagaa                   <  2:144767/151‑1 (MQ=255)                                                                                                                    aagaGCGTTTAAAAGGTGATGATATCTTGGACCCC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGaagaag                  <  2:390952/150‑1 (MQ=255)                                                                                                                    aagaGCGTTAAAAAGGTTATGATATCGTTCACCCC‑GTGCCGCCGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGaagaag                  <  1:185613/150‑1 (MQ=255)                                                                                                                     agggcttttAAAAGGTGATAATTTCGTTGACCCC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAagaaga                 <  2:657061/144‑1 (MQ=255)                                                                                                                          gTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTAACGTTGACTTCACCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATAGCCCGTATACCTTCACCAAAGATCAGATTGAAGCAGAGTTGc            >  1:837214/1‑150 (MQ=255)                                                                                                                           tttCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTg          >  1:222572/1‑151 (MQ=255)                                                                                                                              cAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGttt       >  1:367501/1‑151 (MQ=255)                                                                                                                                  ggTGATGTTATCTTTGCCCCC‑GTGCCGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATg    <  2:757634/150‑1 (MQ=255)                                                                                                                                    taatattatCGTTCACCCC‑GTCACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTAt     <  2:130477/141‑1 (MQ=255)                                                                                                                                      atgatATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGcc  >  1:458548/1‑148 (MQ=255)                                                                                                                                      atgatATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGcc  >  1:791829/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                       ACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTG‑AAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACC‑GTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCC  >  NC_000913/2265493‑2265772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑