Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,526	0	T	G	42.1%	107.8 / 11.2	57	T329P (ACC→CCC)	lacI	lactose‑inducible lac operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (4/29);  new base G (24/0);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 2.72e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCT  >  NC_000913/366377‑366676                                                                                                                                                      |                                                                                                                                                       aaaGCCTGGGGTGCCTAATGAGTGAGCTAACTCTCATTAATTGCGTGTCGCTCGCAGCCCGATTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCAGTTTGCGTATTGGGCGCCAGggtg                                                                                                                                                       <  1:824208/151‑1 (MQ=255)     ccTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCAGGGGGGgtt                                                                                                                                                   >  1:281676/1‑151 (MQ=255)      cTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGCGCCCGGGGGGgttt                                                                                                                                                  >  1:839464/1‑151 (MQ=255)      cTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGCGTTGTGCGGCAGGGGGGgttt                                                                                                                                                  >  1:572369/1‑151 (MQ=255)       tGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTGGGTTTGGGGGCCAGGGGGGttttt                                                                                                                                                 >  1:123770/1‑151 (MQ=255)          ggTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctt                                                                                                                                              <  1:26991/151‑1 (MQ=255)             gCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctttt                                                                                                                                            <  1:593897/150‑1 (MQ=255)                    gagtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGATTTCGCATTTGGCGCCCGGGGGGGTTTTTTTTTCACCCGTg                                                                                                                                    >  1:804262/1‑151 (MQ=255)                        gagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGAc                                                                                                                                <  1:320215/151‑1 (MQ=255)                        gagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTGCACCAGTgaga                                                                                                                                 <  1:522991/150‑1 (MQ=255)                         agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCGGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACg                                                                                                                               <  2:196457/151‑1 (MQ=255)                         agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGGGCCCGGGGGGGTTTTTTTTTTACCCGTGAGACg                                                                                                                               >  1:542555/1‑151 (MQ=255)                           cTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCAGGGGGGTTTTTTTTTTTACCAGTGAGACggg                                                                                                                             >  2:839501/1‑151 (MQ=255)                           cTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGGGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACggg                                                                                                                             >  2:246071/1‑151 (MQ=255)                             aaCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCa                                                                                                                           <  2:361941/151‑1 (MQ=255)                                tCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGGGGGTTTTTTTTTTACCAGGGAGACGGGCaa                                                                                                                          >  2:267402/1‑149 (MQ=255)                                       aaTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAt                                                                                                                  <  2:597476/150‑1 (MQ=255)                                        aTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCCGGGGGGGTTTTTTTTTTACCAGGGAGACGGGCAACAGCTGAtt                                                                                                                 >  2:157204/1‑150 (MQ=255)                                         ttgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCTGAtt                                                                                                                 >  2:221644/1‑149 (MQ=255)                                          tgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGCGCCCGGGGGGTTTTTTTTTTCACCAGTGAGACGGGCCACAGCTGATTGcc                                                                                                              >  2:946005/1‑151 (MQ=255)                                              ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGCTTTCGTTATGGGGGCCCGGGGGGTTTTTCTTTTTCCCCGTGAGAATGGCAACAGGTgtgtggcc                                                                                                             >  1:643987/1‑141 (MQ=255)                                               tgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCAGGGGGGGTTTTTTTTTCACCAGTGAGAAGGGGAAAAGCTGATTGCCCtt                                                                                                           >  2:121112/1‑149 (MQ=255)                                                gcgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGGGGGTTTTCTTTTCAACCGGGAGACGGGGAACAGCTGATTGCCCCTCAc                                                                                                        >  1:108352/1‑151 (MQ=255)                                                  gcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCg                                                                                                      <  2:871368/151‑1 (MQ=255)                                                      aCTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTg                                                                                                  <  2:275026/151‑1 (MQ=255)                                                            cGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTg                                                                                            <  1:744043/151‑1 (MQ=255)                                                                  ccAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGCGCCAGGGGGGTTTTTCTTTTTACCAGTGAGAAGGGCAACAGCTGATTGCCCCTCACCGCCTGGGCCTgagag                                                                                        >  2:172047/1‑149 (MQ=255)                                                                   cAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGGGCCAGGGGGGGTTTTTTTTTTACCAGGGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTTGCCCTGcgagag                                                                                      >  1:942719/1‑150 (MQ=255)                                                                   cAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGt                                                                                     <  2:34856/151‑1 (MQ=255)                                                                    aGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGtt                                                                                    <  2:617951/151‑1 (MQ=255)                                                                          gAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGGGTATTTGGCGCCAGGGGGGGTTTTTTTTTCACCAGGGAGACGGGGAACAGCTGATTGCCCTTCACCCCCTGGCCCTGAGAGAGGTgcag                                                                                >  2:280056/1‑149 (MQ=255)                                                                          gAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgcagca                                                                              <  2:477669/151‑1 (MQ=255)                                                                              ccTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGCGCCCGGGGGGGTTTTTTTTTTACCACGGAGACGGGCAACAGCTGATTGCCCTTCACCCCCTGGCCCTCAGGGAGTTGCAGCaa                                                                             >  1:130609/1‑148 (MQ=255)                                                                                         aGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGCCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTgg                                                               <  1:615823/151‑1 (MQ=255)                                                                                          gCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGt                                                              <  1:742746/151‑1 (MQ=255)                                                                                             gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTg                                                           <  1:105952/151‑1 (MQ=255)                                                                                                       aTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAgg                                                 <  1:738294/151‑1 (MQ=255)                                                                                                         cGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCCGGCCCTGAGAGAGGTGCAGCAAGCGGTCCACGCTGGTTTTCCCCCGCAGGCg                                               >  1:942366/1‑151 (MQ=255)                                                                                                           gCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGaa                                             <  2:888966/151‑1 (MQ=255)                                                                                                             cAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGGGCCAGGGGGGGTTTTCTTTTTCCCAGTGAGAAGGGGAACAGCTGACTGCCCTTCACCCCCTGGCCCCGAGAGAGATGCAGCAAAGGGGCCACGCCGGTTTTCCCCCGCAGGCGaaa                                            >  1:723370/1‑150 (MQ=255)                                                                                                             cAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGCGCCAGGGGGGGTTTTCTTTTTACCAGGGAGAAGGGGCACAGCTGGTTGCCCCTCCCCGCCTGGCCCTTGGAGAGTTGCAGGAAACGGTGCCCGCCGGTTTGCCCCAGCAGGCGgaa                                            >  1:582818/1‑150 (MQ=255)                                                                                                                cgcgcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATcc                                        <  2:936814/151‑1 (MQ=255)                                                                                                                   gcgGGGAGAGGCGGTTTTCGTATTTGGCGCCCGGGGGGGTTTTTTTTTTACCCGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGGTGCAGCAAGCGGTCCCCGCTGGGTTTCCCCCGCAGGCGAAAATcccg                                      >  1:218734/1‑148 (MQ=255)                                                                                                                    cgGGGAGAGGCGGTTTGCGTATTTGGCGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCCGGCCCTGGGAGAGGTGCAGCAAGCGGGCCACGCCGGTTTGCCCCAGCAGGCGGAAAACCTGtt                                    >  1:599838/1‑151 (MQ=255)                                                                                                                    cgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGtt                                    <  1:189705/151‑1 (MQ=255)                                                                                                                          gagGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtgg                              <  1:859634/151‑1 (MQ=255)                                                                                                                                gTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTa                        <  2:930150/151‑1 (MQ=255)                                                                                                                                  ttGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAc                      >  2:29613/1‑151 (MQ=255)                                                                                                                                  ttGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAc                      >  2:430343/1‑151 (MQ=255)                                                                                                                                    gCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcg                     <  1:668599/150‑1 (MQ=255)                                                                                                                                    gCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcg                     <  1:146482/150‑1 (MQ=255)                                                                                                                                      gTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggcg                  <  2:452368/151‑1 (MQ=255)                                                                                                                                          tGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGa               <  1:484865/150‑1 (MQ=255)                                                                                                                                           gggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGata             <  2:410911/151‑1 (MQ=255)                                                                                                                                           gggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGata             <  2:804357/151‑1 (MQ=255)                                                                                                                                            ggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCTCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGatat            <  2:213652/151‑1 (MQ=255)                                                                                                                                                 cAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACa        >  1:798203/1‑150 (MQ=255)                                                                                                                                                   gggTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATg      >  2:939992/1‑150 (MQ=255)                                                                                                                                                     gtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATaa          >  1:452383/1‑144 (MQ=255)                                                                                                                                                      tggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCt  >  1:14833/1‑151 (MQ=255)                                                                                                                                                      tggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAAAATGAg    >  1:535603/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                       AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCT  >  NC_000913/366377‑366676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑