Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913941,7130TG50.0% 68.1 / 23.2 56V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (4/24);  new base G (28/0);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 9.40e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACTACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGG  >  NC_000913/941563‑941859
                                                                                                                                                      |                                                                                                                                                  
gCGACTACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGt                                                                                                                                                    <  1:649991/151‑1 (MQ=255)
       ctcctcCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCtt                                                                                                                                               >  2:370876/1‑149 (MQ=255)
         cctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCtt                                                                                                                                               >  2:833034/1‑147 (MQ=255)
                gcAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCtt                                                                                                                                               >  1:215194/1‑140 (MQ=255)
                  aaaTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTaa                                                                                                                                    >  2:928314/1‑149 (MQ=255)
                        cGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACg                                                                                                                            <  1:412662/151‑1 (MQ=255)
                            aGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAg                                                                                                                        <  1:574219/151‑1 (MQ=255)
                            aGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAAGCAc                                                                                                                             >  1:489707/1‑146 (MQ=255)
                               tttGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTTTCTTTAACAGGCACGCCCGaa                                                                                                                      >  2:114032/1‑150 (MQ=255)
                                 tGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAAt                                                                                                                    <  2:851771/150‑1 (MQ=255)
                                  gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCt                                                                                                                  <  1:62560/151‑1 (MQ=255)
                                  gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGaaa                                                                                                                     >  2:283/1‑148 (MQ=255)
                                     cTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGGCCGCCCGAAAACTaa                                                                                                                >  2:341722/1‑150 (MQ=255)
                                       ataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAAt                                                                                                               <  1:850973/149‑1 (MQ=255)
                                         aCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGccc                                                                                                           <  1:706491/151‑1 (MQ=255)
                                              cggcggCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATg                                                                                                      <  1:606946/151‑1 (MQ=255)
                                               ggcggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGa                                                                                                     <  1:535158/151‑1 (MQ=255)
                                                  ggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGc                                                                                                         >  2:521846/1‑144 (MQ=255)
                                                       ggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcatc                                                                                             <  1:83495/151‑1 (MQ=255)
                                                        gCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAAGCACGCCAGAAAACTAATGCCCGCATGatcatcatc                                                                                             >  1:524078/1‑150 (MQ=255)
                                                          aGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGAt                                                                                          <  1:15118/151‑1 (MQ=255)
                                                          aGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAAAAGGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGa                                                                                           >  1:447164/1‑150 (MQ=255)
                                                           gATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc                                                                                         <  1:296209/151‑1 (MQ=255)
                                                              ggCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCcgc                                                                                      <  2:562817/151‑1 (MQ=255)
                                                              ggCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCcg                                                                                       <  1:683306/150‑1 (MQ=255)
                                                                 aaCAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCt                                                                                   <  2:757671/151‑1 (MQ=255)
                                                                  aCAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAAAGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCta                                                                                  <  2:485169/151‑1 (MQ=255)
                                                                    aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCtata                                                                                <  1:924633/151‑1 (MQ=255)
                                                                        cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATATCTTGAAAAGGCACGCCAGAAAACTAATGCCCGCCTGATCATCATCGATCCGCGCcataac                                                                              >  1:836422/1‑147 (MQ=255)
                                                                         gTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATCTCTTGAACAGGCACGCCCGAAATATCATGACCGCCTGATCAACCTCGATCCCCGCCATAcc                                                                              >  1:277868/1‑148 (MQ=255)
                                                                           cGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGaca                                                                          <  1:99429/150‑1 (MQ=255)
                                                                           cGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGacac                                                                         >  2:378884/1‑151 (MQ=255)
                                                                            ggATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCATCGATCCGCGCCATACCGAAAcc                                                                        >  1:816092/1‑151 (MQ=255)
                                                                                atCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCgg                                                                      >  1:361773/1‑149 (MQ=255)
                                                                                  cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTAATATCTTTAAAAAGCACGCCAGAAAACTAAAGCCCGCATGATCATCATCGATCCGCGCTATACCGAAACCCGGGcc                                                                  >  2:848104/1‑151 (MQ=255)
                                                                                   gAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATATCTTGAACAGGCCCGCCAGAAAACTAATGCCCCCATGATCATCATCGATCCGCGCTATACCGACACCGGTGcc                                                                  >  1:520067/1‑150 (MQ=255)
                                                                                    aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCgg                                                                <  1:206670/151‑1 (MQ=255)
                                                                                       aCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcg                                                             <  2:464282/151‑1 (MQ=255)
                                                                                         aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGggcg                                                           >  2:432308/1‑151 (MQ=255)
                                                                                           tAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGAGGGGGGACTTACTATCTTGAAAAGGCACGCCAGAAAACTAATGCCCGCATGCAAATTATTGCTCCGCGCCATACCGACACCGGTGcc                                                                  >  2:419862/1‑142 (MQ=255)
                                                                                             aGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTTCTATCTTGAAAATGCACGCCAGGAAGCTAATGCCCCCATGATCATCATCGGTCCGCCCCATTTCGGCACCGGGGCCGGGcgcg                                                           >  2:516400/1‑147 (MQ=255)
                                                                                              gCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGAt                                                      <  2:424162/151‑1 (MQ=255)
                                                                                                        cTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTAATATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATGAGGGGGTcc                                            >  1:863504/1‑151 (MQ=255)
                                                                                                          gTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATccc                                           <  2:704477/150‑1 (MQ=255)
                                                                                                                taataaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAAAAGGCACGCCAGAAATCTAAAGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGGAGATGAGTGGATCCCTATTCg                                     >  2:866357/1‑150 (MQ=255)
                                                                                                                    aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAATATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATTATCGATCCGCGCCATACCGACACCGGGGCCGGGCGGGAAGATGAGGGGATCCCTATTCGTCCgg                                >  1:462358/1‑151 (MQ=255)
                                                                                                                       ccTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGaa                             <  2:549511/151‑1 (MQ=255)
                                                                                                                       ccTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGGGCGAAGATGAGTGGGTCCCTATTTGTCCggg                               >  2:432282/1‑149 (MQ=255)
                                                                                                                        cTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAAAAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGCCACCGGTGCCGGGCGCGAAGATGAGGGGATCCCTATTCGTCCGGGaa                             >  2:435517/1‑150 (MQ=255)
                                                                                                                          ggCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACa                           <  2:137239/150‑1 (MQ=255)
                                                                                                                           gCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGa                         <  2:111469/151‑1 (MQ=255)
                                                                                                                             gAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATGAGGGGATCCCTATTCGTCCGGGAACa                           >  2:250134/1‑147 (MQ=255)
                                                                                                                             gAAACGCGAATGAGTGGCGGTGGGGGGACTTAATATCTTTAAAAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCCCGCCATACCCACACCGGTGCCGGGCGCGGAGATGAGTGGGTCCCTATTCGTCCGGGGACAGAt                        >  1:639351/1‑150 (MQ=255)
                                                                                                                             gAAACGCGAATGAGTGGCGGGGGGGGGACTTACTATTTTGAAAAGGCACGCCAGAAATCTAATGGCCGCAAGATCATCATCGATCCGCGCCATACCCACACCCGGGCCGGGCGCGAAAATGGGTGGATCCCTATTCGTCCcggga                             >  1:688345/1‑143 (MQ=255)
                                                                                                                              aaaCGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGa                         >  2:256363/1‑148 (MQ=255)
                                                                                                                                  gcgAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCCACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGc                   >  2:778484/1‑150 (MQ=255)
                                                                                                                                                  ggggTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTgg  >  1:893343/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GCGACTACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGG  >  NC_000913/941563‑941859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: