Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,895,843	A→C	V256G (GTG→GGG)	adeP ←	adenine permease, high affinity; adenine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,895,843	0	A	C	100.0%	214.1 / NA	56	V256G (GTG→GGG)	adeP	adenine permease, high affinity; adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/32);  total (24/32)

CCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACC  >  NC_000913/3895696‑3895986                                                                                                                                                    |                                                                                                                                                ccGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCcccc                                                                                                                                              >  1:790488/1‑151 (MQ=255)  cGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATcccccc                                                                                                                                             >  2:577439/1‑151 (MQ=255)   ggTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCt                                                                                                                                            <  1:218930/151‑1 (MQ=255)      cACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGcc                                                                                                                                         >  2:559679/1‑151 (MQ=255)            agagATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCg                                                                                                                                   <  1:107871/151‑1 (MQ=255)            agagATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCg                                                                                                                                   <  2:841231/151‑1 (MQ=255)             gagaTACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGa                                                                                                                                  <  2:817423/151‑1 (MQ=255)                aTACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGt                                                                                                                               <  2:318784/151‑1 (MQ=255)                 tACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGtt                                                                                                                              >  1:664020/1‑151 (MQ=255)                     gTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAAc                                                                                                                          >  1:424046/1‑151 (MQ=255)                               aGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTa                                                                                                                <  1:726970/151‑1 (MQ=255)                                    cTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCt                                                                                                           >  2:873909/1‑151 (MQ=255)                                      gCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTAc                                                                                                         >  2:793649/1‑151 (MQ=255)                                          cATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATg                                                                                                      >  2:495835/1‑150 (MQ=255)                                               gcgGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCcac                                                                                                <  2:482527/151‑1 (MQ=255)                                                     aTTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGt                                                                                          >  2:618330/1‑151 (MQ=255)                                                      tttCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTc                                                                                         >  1:236890/1‑151 (MQ=255)                                                           ccTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTAc                                                                                    >  1:807389/1‑151 (MQ=255)                                                               ctcATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTc                                                                                >  2:113623/1‑151 (MQ=255)                                                                 cATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCt                                                                                 <  2:330455/148‑1 (MQ=255)                                                                 cATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCt                                                                                 <  2:939093/148‑1 (MQ=255)                                                                  aTCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTcggc                                                                             <  2:541705/151‑1 (MQ=255)                                                                  aTCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTcggc                                                                             <  1:530860/151‑1 (MQ=255)                                                                   tCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTcggcg                                                                            >  1:169632/1‑151 (MQ=255)                                                                     cGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCggcggc                                                                          >  1:127381/1‑151 (MQ=255)                                                                     cGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCggcggc                                                                          <  2:579713/151‑1 (MQ=255)                                                                       ccAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGgcgc                                                                        <  2:183838/151‑1 (MQ=255)                                                                               gCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACg                                                                 >  2:937671/1‑150 (MQ=255)                                                                                       ggTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTg                                                        >  1:208908/1‑151 (MQ=255)                                                                                       ggTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTg                                                        <  2:476907/151‑1 (MQ=255)                                                                                        gTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGt                                                       <  1:574525/151‑1 (MQ=255)                                                                                          cACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGt                                                       >  2:334210/1‑149 (MQ=255)                                                                                          cACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAg                                                     <  2:14047/151‑1 (MQ=255)                                                                                             gCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGc                                                  <  2:162324/151‑1 (MQ=255)                                                                                              ccAATCAGCGTACCCGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGca                                                 <  1:815108/151‑1 (MQ=255)                                                                                                   cAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGCCCCACAACTGTCATTGCGCTCGGGGGCGCAGAAACGATGCCATTGTAGTGCCCAt                                              <  1:252332/149‑1 (MQ=255)                                                                                                   cAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCa                                            <  2:139271/151‑1 (MQ=255)                                                                                                         aCCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGc                                      >  1:425995/1‑151 (MQ=255)                                                                                                             gaggagTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATcc                                  >  2:338938/1‑151 (MQ=255)                                                                                                             gaggagTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATcc                                  <  1:760930/151‑1 (MQ=255)                                                                                                             gaggagTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATcc                                  <  2:174785/151‑1 (MQ=255)                                                                                                             gaggagTCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATcc                                  >  2:80026/1‑151 (MQ=255)                                                                                                               ggagCCAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATccag                                <  2:872688/151‑1 (MQ=255)                                                                                                                    cAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATccagccca                            <  2:208415/150‑1 (MQ=255)                                                                                                                    cAAACAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATccagccca                            <  2:485572/150‑1 (MQ=255)                                                                                                                      aaCAAATTGGCCAACATGAAAGGGAAAAACCCCCCCGCCAGCCCGAGGTTAAACGGCCCGGCTAAAACTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCcagca                         <  1:941112/151‑1 (MQ=255)                                                                                                                       aCAAGTTGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCcagca                         <  1:565658/150‑1 (MQ=255)                                                                                                                            ttGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGcgtcg                   >  2:92898/1‑151 (MQ=255)                                                                                                                             tGACCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGcgtcg                   <  1:479701/150‑1 (MQ=255)                                                                                                                               aCCAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTca                <  1:494164/151‑1 (MQ=255)                                                                                                                                ccAACATGAAAGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTcac               >  1:446257/1‑151 (MQ=255)                                                                                                                                        aaaGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGAt         >  1:864132/1‑149 (MQ=255)                                                                                                                                        aaaGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAg       <  1:31016/151‑1 (MQ=255)                                                                                                                                        aaaGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAg       <  1:589950/151‑1 (MQ=255)                                                                                                                                         aaGAGAAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGAt         >  2:112841/1‑148 (MQ=255)                                                                                                                                            agaAAATCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAAc   <  1:242275/151‑1 (MQ=255)                                                                                                                                               aaaTCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAAcc  >  2:50025/1‑149 (MQ=255)                                                                                                                                               aaaTCCCCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAAcc  >  1:133295/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                CCGGTCACGGAAGAGATACTGTCGACATACAGCGCCTGCTTCATGCGCGGGAATTTCCCCTTCTCATCCGCCAGACCTGCTTTATCGGTCACGCCAATCAGCGTACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACC  >  NC_000913/3895696‑3895986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑