Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,592	0	G	T	45.0%	80.7 / 32.4	60	L136F (TTG→TTT)	yegJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (6/27);  new base T (27/0);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.47e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC  >  NC_000913/2151443‑2151735                                                                                                                                                        |                                                                                                                                                   aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTgg                                                                                                                                                   <  2:83056/151‑1 (MQ=255)     ttATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAA‑CAATTGAACTTTTTTTgtgcc                                                                                                                                                >  2:920178/1‑147 (MQ=255)      tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑A‑CTATTGATCCTTTTTTTGTGAcc                                                                                                                                              >  2:514193/1‑151 (MQ=255)       aTTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑T‑CCATTTTTCCTTTTTTTGTTAcc                                                                                                                                              >  1:279724/1‑150 (MQ=255)              tAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑T‑CCATTGATCCTTTTTTTGtt                                                                                                                                                 >  1:552457/1‑139 (MQ=255)               aaTCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCaaaa                                                                                                                                    <  2:649925/151‑1 (MQ=255)                  cAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAaaga                                                                                                                                  <  2:991532/150‑1 (MQ=255)                         gACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGcc                                                                                                                           <  1:443935/150‑1 (MQ=255)                           cccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCcgac                                                                                                                        <  2:654705/151‑1 (MQ=255)                                  aTTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTaa                                                                                                                 <  2:372781/151‑1 (MQ=255)                                  aTTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTa                                                                                                                  <  1:514587/150‑1 (MQ=255)                                     gTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAaga                                                                                                               <  2:448148/150‑1 (MQ=255)                                        aCAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑Ca                                                                                                           <  2:788162/150‑1 (MQ=255)                                         cAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑Caaa                                                                                                         <  1:311110/151‑1 (MQ=255)                                         cAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑Caaa                                                                                                         <  1:644562/151‑1 (MQ=255)                                         cAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑ACATTTATTCTTTTTTTGGTACCTA‑TCCCAAAAAAGAAACAGACGAGCCAAGAG‑aaa                                                                                                          >  2:631753/1‑150 (MQ=255)                                           aaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑A‑TTAATTTTCCCTTATTTGTTTCCTA‑TTCCAAAAAAAAAGCCGACGTGGTAAAAG‑Caaaa                                                                                                        >  2:70474/1‑150 (MQ=255)                                           aaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑A‑ACAATTTTTCTTTTTTTGTGACCCA‑TTCAAAAAAAAAAAACGACGAGCCAAAAG‑Caaaa                                                                                                        >  2:340639/1‑150 (MQ=255)                                            aCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑ACATTTATTCTTTTTTTGTTACCCA‑TCCCAAAAAAAAAACCGACGAGACAAAAG‑Gaaaa                                                                                                        >  2:643187/1‑149 (MQ=255)                                                aGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑ACATTTTTTCTTTTTTTGTCACCTA‑TCCAAAAAAAAAAAACGACGAGCGAAGAG‑Caaaa                                                                                                        >  2:192219/1‑145 (MQ=255)                                                 gAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTc                                                                                                 <  1:914570/151‑1 (MQ=255)                                                  aaTATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑T‑ACCTTTATTCTTTATTTGTCACCTC‑ACCCAAAAAAAAAACCGACGAGCCAAGAG‑Caaaaat                                                                                                      >  1:218304/1‑143 (MQ=255)                                                   atatGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑ACCTTTGTTCTTTTTTTGTGACCTATTCCAAAAAAAAAAACC‑ACGCGCTAAAAGACAAAAtaattt                                                                                                  >  2:345193/1‑144 (MQ=255)                                                    tatGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑A‑ACCATGTTCCTTTTTTTGTTACCTA‑TTCAAAAAAAAAAAACGACGAGCTaaaaaaaaaa                                                                                                         >  2:168116/1‑133 (MQ=255)                                                        gCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑A‑ACATTTATTCTTTTTTTGTTCCCCA‑TCCCAAAAAAGAAACCCACGAGCTAAGAG‑CAAAAATATTTCGCTaa                                                                                            >  1:302849/1‑149 (MQ=255)                                                        gCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATc                                                                                          <  1:847658/151‑1 (MQ=255)                                                          cAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑ACATTTATTCTTTTTTTGTTAACCA‑TCCCAAAAAAAAAAACGACGAGCTAAGAA‑GAAAAATAATTTGCTaa                                                                                            >  2:36899/1‑147 (MQ=255)                                                             gAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCg                                                                                     <  2:226815/151‑1 (MQ=255)                                                                 ggAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCCTTTTTTCTTTTTTTGTCACCCA‑TCCAAAAAAAAAAACCGACGAGCTAAAAG‑CAAAAATTGTTTACTaaatatgcggta                                                                                  >  1:534719/1‑140 (MQ=255)                                                                 ggAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTtat                                                                                 <  2:541859/151‑1 (MQ=255)                                                                 ggAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTtat                                                                                 <  2:442504/151‑1 (MQ=255)                                                                 ggAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTtat                                                                                 <  1:735625/151‑1 (MQ=255)                                                                 ggAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑ACAATTATTCTTTTTTTGGTTCCTA‑TTCAAAAAAAAAAAACGACGAAGTAAAAG‑CAAAAATAATTTTCTaaaaaacc                                                                                      >  2:376017/1‑140 (MQ=255)                                                                    aaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGATTT‑A‑TCCTTTTTACCTTTTTTGTTAACTT‑TCCCAAAAAAAAAACCGCAGCGCGAAAAG‑CAAAAATAGATTGCTAAATAGccccgtt                                                                                 >  1:423081/1‑143 (MQ=255)                                                                     aaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑ACAATTATTCTTTTTTGGTTACCTA‑TCCCAAAAAAGAAAACCACGAGCTAAGCG‑CAAAAATAGTTCGCTAAttaacccgtttattt                                                                             >  1:294664/1‑137 (MQ=255)                                                                       tCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGATAT‑ATCCATT‑ATCCTTTTTTTGTCTCCCA‑TCCAAAAAAAAGAGACGACCAGGTAAAAA‑CAAAAATAGTTTGCGAAACAACCAGTAtattt                                                                             >  1:296133/1‑149 (MQ=255)                                                                        cAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTAtatttatt                                                                          <  2:9832/151‑1 (MQ=255)                                                                          agaaagaaGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑A‑ACATTGATTCTTTTTTTGTGACCTA‑TTCAAAAAAAGAAAACGACGAGCTAAAAG‑CAAAAATAATTCGCTAAACAACCGGTTtatttttt                                                                          >  2:99036/1‑149 (MQ=255)                                                                          agaaagaaGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGt                                                                        <  1:970502/151‑1 (MQ=255)                                                                             aagaagATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTTATCCTTTATTTGTCACCCA‑TCCCAAAAAAGAAGACGACGAGCTAAAAG‑CAAAAATATTTCGCTAAACAGCCGGTTTATTTAtttttt                                                                      >  1:513695/1‑150 (MQ=255)                                                                                aagaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑C‑CCCTTTCTTCCTTATTTGTGACCAA‑ACCCAAAAAAAAAAACGAACAGCTAAGAG‑CAAAAATAACTCACTCATCAGCCGCTCTAtttttttttt                                                                      >  2:808149/1‑147 (MQ=255)                                                                                 agaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑ACAATTATTCTTTTTTTGTGACCTA‑TTCAAAAAAAAAAAACGACGAGCTAAAAG‑CAAAAATAATTTCCTTATAAAACGGTATATTTTTTTTTTtaa                                                                   >  2:946824/1‑149 (MQ=255)                                                                                   atatTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTgg                                                               <  2:841147/151‑1 (MQ=255)                                                                                         cAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑A‑CCATTGATCCTTTATTTGTCACCTA‑TCCAAAAGAAGAAAACGGCGAGCGAAAAG‑CAAAAATAATTTGCTAATCAACCGGTATATTTTTTTTTTTAAAAGGC‑ggg                                                          >  1:175317/1‑148 (MQ=255)                                                                                         cAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑A‑ACATTTTTTCTTTTTTTGTTACCTA‑TTCAAAAAAAAAAGCCGGCCAGCTTAAAG‑CAAAAATAATTCGCTTAACAACCGGTTTTTTTATTTTTTtaaaatgcggg                                                           >  2:53920/1‑141 (MQ=255)                                                                                          aGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTTATCCTTTTTTTGTGACCCA‑TCCAAAAAAAAAAAACGACGAGCCAAGAG‑CAAAAATAGTTTGCTAATAAACCGGTATATTTTTTTTTTTAAATGTC‑ggg                                                          >  2:526499/1‑147 (MQ=255)                                                                                          aGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGa                                                       <  2:571388/151‑1 (MQ=255)                                                                                            aCTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAAt                                                     <  1:720874/151‑1 (MQ=255)                                                                                               ggATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAAttg                                                   <  1:514251/150‑1 (MQ=255)                                                                                               ggATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAAttg                                                   <  2:81985/150‑1 (MQ=255)                                                                                                     ataCGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCtt                                            <  2:203729/151‑1 (MQ=255)                                                                                                     ataCGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑ACACTTATCCTTTTTTTGTTCCCTC‑TCCCAAAGAAAAAACCGACCAGGTCAGAA‑CAAAAATAATTTGCTAATCAGCCCCTATATTTTTTTTTTTAAATGGC‑gggtttaattttttttt                                            >  1:554510/1‑136 (MQ=255)                                                                                                        cgcgcgGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCaa                                         <  1:715383/151‑1 (MQ=255)                                                                                                             ggggATAAAATTTACGGCGGTTAT‑A‑ACATTTATTCTTTTTTTGTCACCTA‑TCCAAAAAAAAAAACCGACGAGCTAAGAG‑CAAAAATAGTTTTCTAATCAGCCGTTTTATTTTTTTTTTTAAATGGCGGGTTGATTTTTTTTTTTTAAcccc                                     >  1:981587/1‑149 (MQ=255)                                                                                                              gggATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCa                                   <  1:36904/151‑1 (MQ=255)                                                                                                                      aTTTACGGCGGTTAT‑A‑CCATTGATCCTTTTTTTGTTACCTA‑TTCAAAAAAAGAAAACGACGAGCTAAGAG‑CAAAATTAGTTTGCTAATAAGCCGTTTTATTTTTTTTTTTAAATAGC‑GGGTGTAttttttttttttaccccc                                    >  2:583641/1‑129 (MQ=255)                                                                                                                            ggcggTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTTTCTTTAACCCCCATGAGaaa                            >  2:57945/1‑144 (MQ=255)                                                                                                                             gcggTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCActct                    >  2:254998/1‑151 (MQ=255)                                                                                                                               ggTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTTTCTTCAACCGCCATGAGAAACTCActct                    >  1:600006/1‑149 (MQ=255)                                                                                                                                               tCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTg    >  1:88109/1‑151 (MQ=255)                                                                                                                                                ccTTTATTGGTCACTTA‑TCAAAAAGAAGAACCCGACGAGTTAAGAG‑AAAATTTAGTTCGCTAATCAGCCGTTTTTTTTATTGTTTAACCGGGC‑GGTTGAATTGTTGTCTTCAGCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGc   <  2:610371/151‑1 (MQ=255)                                                                                                                                                  tttATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc  >  1:978415/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                   ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑A‑CCATTGATCCTTTATTGGTCACCTA‑TCCAAAAGAAGAAGCCGACGAGCTAAGAG‑CAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC  >  NC_000913/2151443‑2151735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑