| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,578,143 | 0 | A | C | 46.6% | 90.5 / 17.9 | 58 | L79V (TTA→GTA) | maeB | malic enzyme: putative oxidoreductase/phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base A (26/5); new base C (0/27); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.16e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCG > NC_000913/2577994‑2578281 | tggttCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTaa < 2:299566/150‑1 (MQ=255) gttCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAAccc > 1:190456/1‑151 (MQ=255) cGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAAcccc > 1:430165/1‑149 (MQ=255) gcggcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCAccg < 1:842813/151‑1 (MQ=255) ggcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCAccgcc < 1:501182/151‑1 (MQ=255) ggcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCAccgcc < 1:462623/151‑1 (MQ=255) cgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGt > 1:390964/1‑151 (MQ=255) gACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGt > 2:393902/1‑150 (MQ=255) aCAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTAATTGTCAAATCCACCAACCCCGGCGATTTTCTTAAACAAAGCGCCCTGGCCTTCCATCCCCGATTTGCCTCCCAGCGCCCCAATGTTG‑CCAACCCCCACCACCGCCGt < 2:492083/149‑1 (MQ=255) aaTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTag > 1:4632/1‑149 (MQ=255) aaaTTTGTCCGGGTCGAGTTCGTCAACTTAAATGTCAAATACATCAACCCCGGCGATTTTCTTAAACAGACCGCCCTTGCCTCCCACCCCCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATc < 1:647896/151‑1 (MQ=255) gggTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGccacc > 1:457409/1‑150 (MQ=255) gAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAgg > 2:344002/1‑148 (MQ=255) gAGTTCCTCAACTTAAATGTAAAATACATCAACCCCGGCGATTTTCTTAAACAGACCCCCTTTGCCTTCCACCCCCGTTTTCCCTCCCGGCGCGCAAATGTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTa < 1:413138/151‑1 (MQ=255) atttCGTCAACTTAAATGTCAAATACACCACCCCCGGCAAATTTCTTAAACAGACCGCCCTTCCCTCCCATCACCGGTTTGCCTCCCAGCGCCCCAATGTCG‑CCAACCCCCACCACCGCCGTACCGTTAGAGATCACCGCCACCAGGt < 2:296197/146‑1 (MQ=255) ttCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCt > 2:360265/1‑151 (MQ=255) aaCTTCAATGTAAAATACACCACCCCCGGCGAATTTCTTAAACAGAACCCCCTTCCCTTCCATCGCCGTTTTCCCTGCCAGCGCGCAATTGTTC‑CCAACCCCCAGCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCgg < 1:612656/148‑1 (MQ=255) aaCTTAAATGTCAAATACATCACCCCCGGCAATTTTCTAAAACAGACCCCCCTTCCCTCCCACCCCCGTTTTGCCTGCCAGCGCGCAATTGTCC‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTc < 2:216270/146‑1 (MQ=255) aaCTCCAATGTAAAATACATCAACCCCGGCGATTTTCTAAAACAGACCCCCCTTCCCTCCCACCCCCGGTTGGCCTGCCAGCGCGCAAATTTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGc < 1:443110/150‑1 (MQ=255) aCTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCgg > 2:46520/1‑151 (MQ=255) aCTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCgg > 2:783868/1‑151 (MQ=255) ttCAATGTAAAATACATCAACCCCGGCGATTTTCTTAAACAGACCCCCCTTGCCTCCCATCCCCGGTTTGCCTGCCGGCGCGCCAATGTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCgg < 1:247102/149‑1 (MQ=255) tCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGTCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat > 2:435142/1‑151 (MQ=255) cAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat > 2:37314/1‑150 (MQ=255) aaTGTAAAATACATCAACCCCGGCAAATTTCTTAAACAGACCGCCCTTGCTTTCCATCCCCGGTTTGCCTGCCAGCGCGCAAATTTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTatat < 1:574817/151‑1 (MQ=255) aaTGTAAAATACACCAACCCCGGCGATTTTCTAAAACAGACCCCCCTTCCCTCCCACCCCCGGTTTCCCTGCCAGCGCCCAAATGTGC‑CCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTatat < 2:405477/151‑1 (MQ=255) gtaaaaTCCATCAACCCCGGCGATTTTCTAAAACAGACCGCCCTTGCCTTCCACCCCCGTTTTGCCTGCCAGCGCCCCATTGTTC‑CCTACCCCCAGCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAtt < 2:136339/146‑1 (MQ=255) aaaaccacaaaccccGGCAATTTTCTAAAACAGACCCCCCTTGCCTCCCACCCCCGGTTGGCCGGCCGGCCCCCAAATGTTC‑CCAACCCCAAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAg < 2:35073/139‑1 (MQ=255) aaaTCCATCATCCCGGGCAAATTCCTTAAACAGACCCCCCTTCCCTTCCACCCCCGGTTGGCCGGCCAG‑GCCCCCATGGTGCCCTACCCCCGGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg < 2:457474/148‑1 (MQ=255) tACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCt > 1:379739/1‑151 (MQ=255) accAACCCCGGCAAATTTCTAAAAAAAAACGCCTTTGCCTCCAACCACGGTTTGGCCGGCAAGCGCGCAAATGTGG‑CCAAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGc < 2:485573/145‑1 (MQ=255) aaTCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAAc > 1:953759/1‑151 (MQ=255) aaTCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAAc > 2:545778/1‑151 (MQ=255) aaTCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAAc > 2:798794/1‑151 (MQ=255) aaTCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAAc > 2:945261/1‑151 (MQ=255) ccccGGCGATTTTTTTAAACAGACCGCCCTTGCCTCCCACCCCGGGTTTGCCTGCCAGCGCCCAAATTTTC‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACgg < 1:736454/150‑1 (MQ=255) cccGGCGATTTTCTTAAACAGACCGCCCTTGCCTTCCATCCCCGTTTTGCCTGCCAGCGCGCAAATTTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACg < 1:853691/149‑1 (MQ=255) cGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTc > 2:156324/1‑151 (MQ=255) aaTTTCTTAAACAGACCGCCCTTGCCTTCCATCCCCGGTTTGCCTCCCGGCGCGCAAATTTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtt < 1:535918/148‑1 (MQ=255) aaaaAGACCGCCTTTCCTTCCCACCCCCGGTTTGCCTCCCAGCGCCCAATTTTTC‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTc < 2:918872/150‑1 (MQ=255) aaaCAGACCCCCCTTCCCTCCCACCCCCGTTTTGCCTGCCAGCGCGCCAATGTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAt < 1:838842/147‑1 (MQ=255) aaaCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTc > 1:802035/1‑150 (MQ=255) aaCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa > 2:560428/1‑150 (MQ=255) aCAGAACGCCCTTGCCTCCCACCCCCGGTTTGCCTGCCAGCGCGCAATTGTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAg < 2:404275/151‑1 (MQ=255) accccccttcccttCCACCCCCGTTTTGCCTGCCAGCGCCCCAATTTGC‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACa < 1:584060/146‑1 (MQ=255) aaCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACa > 2:923546/1‑150 (MQ=255) cccTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTg > 1:563099/1‑151 (MQ=255) ccTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCa > 1:794780/1‑150 (MQ=255) ccTCCCACCCCCGGTTGCCCTCCCGGCGCCCCAATTTTC‑CCAACCCCAAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCg < 1:203090/147‑1 (MQ=255) accccggttttCCCTGCCAGCGCCCCAATGTTC‑CCAACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAAc < 2:913083/138‑1 (MQ=255) ggTTTGCCTCCCAGCGCGCAAATTTTC‑CCTACCCCCGGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGa < 1:977829/149‑1 (MQ=255) ggTTTGCCGGCCGGCGCGCAAATGTTG‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGa < 1:928794/149‑1 (MQ=255) gcctgccAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGcc > 1:723974/1‑151 (MQ=255) ccAGCGCCCCAATGTTG‑CCTACCCCCGGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAgcgc < 1:300138/151‑1 (MQ=255) ccAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgc > 1:376211/1‑151 (MQ=255) ccAATGTTC‑CCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGa < 1:154469/147‑1 (MQ=255) cAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgcg > 1:114630/1‑151 (MQ=255) cAATGTCC‑CCTAACCCAAACGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgc < 2:953643/150‑1 (MQ=255) ttcccTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgcg < 2:821346/143‑1 (MQ=255) | TGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTG‑CCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCG > NC_000913/2577994‑2578281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |