Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,624,517 | 0 | G | T | 41.4% | 71.4 / 26.7 | 59 | E492* (GAA→TAA) | ppk | polyphosphate kinase, component of RNA degradosome |
Reads supporting (aligned to +/- strand): ref base G (12/22); new base T (24/0); total (37/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.44e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCG > NC_000913/2624371‑2624667 | gTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTAATTTATTTaaa > 2:792292/1‑150 (MQ=255) aaaaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATa < 1:159446/151‑1 (MQ=255) aaaaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTACTTTTTTTAAAAacc > 1:559817/1‑148 (MQ=255) aaaaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTAACTTTATTTAAAAacc > 1:290598/1‑148 (MQ=255) aaaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTTAAAACCCATa > 2:403259/1‑150 (MQ=255) aaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTAACTTTATTTAAAAACCACAcc > 1:300441/1‑151 (MQ=255) aaGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCgg < 2:983454/150‑1 (MQ=255) gCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACAtt < 1:304762/150‑1 (MQ=255) gTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGa < 1:237340/151‑1 (MQ=255) ttATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAAATTTTTTTAAAAACCCCACCGTCCCGGGACATTTGat > 2:850981/1‑151 (MQ=255) tATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTAACTTTATTGAAAACCCATACCGTCCGGGGGCATTTGatt > 1:770922/1‑151 (MQ=255) acctCGGGACCGGGAACTTTAACGAAAAACCCGCGCGTCTTTATCCTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTa < 2:280226/146‑1 (MQ=255) acaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAAt < 1:524324/151‑1 (MQ=255) acaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTAACTTTATTTAAAACCCATACCGTCCGGGGGCATTTGATTAttt > 1:114218/1‑148 (MQ=255) caTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTAATTTTTTTTAAAACCC‑TACCCTCCGGGGGCAATTTTTTAttt > 2:703849/1‑147 (MQ=255) caTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGAACGCCGATGCGCGCATCCCCAACGAAGTACGGCGGGAT‑CTTCACCTTCTTTTGAACACAAACCGGCAGGTGATGTTTGATTGttt > 2:536601/1‑147 (MQ=255) cGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTa < 1:120873/151‑1 (MQ=255) gggACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTAt < 2:644036/151‑1 (MQ=255) gggAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTTAAAACCCACACCGTCCGGGGACATTTTATTATTTAATGGGATtgcccc > 2:365842/1‑149 (MQ=255) tttAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTTAATTTATTTTAAAACCATAACGTCCGGGGGCAATTTATTATTTTATGGTATTGCCGCaaaa > 1:631474/1‑149 (MQ=255) ttAACGAAAAACCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACt < 1:67791/150‑1 (MQ=255) aaCGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTccc < 2:884139/151‑1 (MQ=255) aaaaaaCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTACTTTTTTTAAAACCCCTACCGCCCGGGGGCATTTTATTATTTTATTGTATTGCCCCCAAACTCccgcc > 2:382378/1‑150 (MQ=255) aaaCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTACTTTTTTTAAAAACCCTACCCTCCGGGGACATTTTATTTTTTTATGGTATCGCCGCAAAACTAcccccccc > 2:450618/1‑150 (MQ=255) aaCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTa < 2:519740/151‑1 (MQ=255) aCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTTAATTTTTTTAAAAACCATACCGGCCGGGGACCATTTATTATTTTATGGTATCGCCGCCAAAATCcccccccc > 1:508816/1‑148 (MQ=255) gcgcgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTAACTTTATTTAAAACCCAAACCGTCCGGGGGCAGTTTATTTTTTAATTGTATTGCCGCAAAAATCCCCCCGCCCATTGt > 1:816177/1‑151 (MQ=255) cgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGT‑TTTAAATTTTTTTAAAACCCAAACCCTCCGGGGACATTTGATTATTTTATTGTATCGCCGCAAAAATCCCGCCGCCTTTTGt > 1:204277/1‑148 (MQ=255) tCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGa < 2:49103/150‑1 (MQ=255) tCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTACTTTTTTGAAAAACCATACCGTCCGGGGACATTTGTTTATTTTATTGGATCGCCGCCAAAATCCCCCCGCCTCTTGTAt > 1:522180/1‑148 (MQ=255) cTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTAATTTTTTTAAAAACCATACCCCCCGGGGACATTTTATTATTTAATGGTATCGCCGCAAAAATCCCCCCGCCTATTTTATGaa > 1:742352/1‑150 (MQ=255) tttATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTAACTTTTTTTAAAAACCATACCGGCCGGTGACCTTTGATTTTTTAATGGTATCGCCGCCAAACTCCCGCCGCCCATTGTATTaaa > 1:463866/1‑150 (MQ=255) tGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTTACTTTTTTGTAAAACCCTACCCGCCGGGGGCATTTTATTTTTTTATGGGATCGCCGCAAAACTCCCCCCGCCCATTGTATGAAAaggggg > 2:776306/1‑149 (MQ=255) tATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTTACTTTTTTGTAAACCCCCACCCTCCGGGGGCATTTTATTTTTTTATGGTATCGCCGCAAAAATCCCGCCGCCCATTTTATGAAAAGGGGGACcgc > 2:794804/1‑150 (MQ=255) tATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTACTTTATTTAAAAACCATACCGTCCGGGGGCATTTTATTTTTTAATGGTATCGCCCCCAAACTCCCGCCGCCTATTGTATGAAAAGGTGGACcgc > 1:985697/1‑150 (MQ=255) tCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCAACTGTATGAAATGGTGGACCGCgag < 2:207879/150‑1 (MQ=255) gTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTACTTTTTTTAAAACCCCTACCGTCCGGGGACCTTTTATTATTTTATGGTATCGCCGCAAAAATCCCCCCGCCCATTGTATGAAAAGGTGGGCCGCGGGATc > 2:843944/1‑151 (MQ=255) tGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTAATTTATTTAAAAACCATACCCTCCGGGGAAATTTGATTTTTTAATGGTATCGCCCCAAAAATCCCCCCGCCCTTTGTATGAAATGGGGGACCGCGAGATCGc > 1:897791/1‑151 (MQ=255) gCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTTACTTTTTTTAAAACCCATAACGTCCGGGGACATTTTATTATTTTATGGGATTGCCGCAAAAATCCCGCCGCCTATTGTATGAAATGGTGGGCCGCGAGATCGcc > 1:822979/1‑151 (MQ=255) gCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAAATTTATTGTAAAACCACAACGTCCGGTGGCATTTGATTATTTAATGGGATTGCCGCAAAAATCCCCCCCCCTATTGTATGAAAAGGTGGGCCGCGAGAtgacc > 2:387682/1‑147 (MQ=255) aCCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTTAATTTATTGAAAACCCCTACCGTCCGGGGGCATTTGATTATTTAATGGTATCGCCGCAAAAATCCCCCCGCCCATTTTATGAAAAGGTGGACCGCGAGATCGCCAAc > 2:386239/1‑150 (MQ=255) aCCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAAcg < 1:591134/151‑1 (MQ=255) aCCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAAATTTTTTTAAAAACCATACCCTCCGGTGACATTTTATTATTTTATGGTATCGCCGCAAAAATCCCGCCGCCTATTGTTTGAAATGGGGGGCCGCGAGATCGCCAAc > 1:950149/1‑150 (MQ=255) ccgccgATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTTACTTTATTGTAAACCCCTACCCTCCGGGGGCCTTTTGTTTTTTTATGGTATTGCCGCCAAAACCCCCCCCCCCATTTTTTGAAAATGTGGGCCCCGCGATCGccc > 1:699394/1‑146 (MQ=255) gccgATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACgcgc < 2:970560/151‑1 (MQ=255) cgATGCGCGCATCACCAACGAAGTACGGCGGGGA‑TTTAAATTTTTTGAAAACCCCTACCGTCCGGGGACATTTGATTTTTTAATGGGATCGCCGCAAAACTCCCGCCGCCTATTTTTTGAAATGGTGGACCGCGAGATCGCCAAcac > 1:639251/1‑145 (MQ=255) aTGCGCGCATCACCAACGAAGTACGGCGGGTATTTTAATTTTTTTAAAAACCCA‑ACCGTCCGGGGGCATTTGATTTTTTTATTGTATCGCCGCCAAACTCCCGCCGCCCATTGTATGAAATGGTGGGCCGCGGGATCGCCCACgcgc > 1:711221/1‑147 (MQ=255) cgcgcATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAgg < 1:151062/151‑1 (MQ=255) cgcgcATCACCAACGAAGTACGGCGGGGA‑TTTAACTTTATTGAAAACCCAAAACGTCCGGGGACATTTGATTATTTAATGGTATCGCCGCCAAACTCCCGCCGCCCATTGTTTGAAATGGGGGACCGCGAGATCGCCAACCCCCCGCAAgg > 2:480572/1‑151 (MQ=255) gcgcATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTTTAAAACCATACCGACCGGGGACATTTTATTTTTTTATTGGATCGCCGCCAAACTCCCCCCCCCTTTTTTTTTAAATGGTGGGACGCGAGAGCCCCAACCCGCAGCAAagg > 1:629753/1‑151 (MQ=255) gcATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCt < 2:382877/151‑1 (MQ=255) gcATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCt < 2:629684/151‑1 (MQ=255) cACCAACGAAGTACGGCGGGTA‑TTTTACTTTTTTGAAAAACCCTACCCTCCGGGGACATTTTATTTTTTTATTGGATTGCCCCAAAACTCCCCCCCCCCATTGTTTGAAAATGTGGGCCGCGGGAACGCCAACCCGCAGCCAGGGGCGccc > 2:383088/1‑151 (MQ=255) aaGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTAt < 1:749032/151‑1 (MQ=255) gggTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGaa < 1:88230/151‑1 (MQ=255) a‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTa < 1:342449/151‑1 (MQ=255) ttAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTaa < 1:408636/150‑1 (MQ=255) ttAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTaa > 1:47316/1‑150 (MQ=255) tGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTc > 2:32057/1‑151 (MQ=255) gAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCg > 1:536542/1‑151 (MQ=255) | GTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTA‑TTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCG > NC_000913/2624371‑2624667 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |